LOCUS       pDONRP2R.P3-mScarlet-HOTag3        3617 bp ds-DNA     circular SYN 28-SEP-2023
DEFINITION  .
ACCESSION   urn.local...7d-gszzt46
KEYWORDS    .
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   alsaf
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2019 from SnapGene 4.2.0 to Vector NTI(R) format
            http://www.snapgene.com
COMMENT     VNTDATE|876700800|.
FEATURES             Location/Qualifiers
     attR2           complement(1..126)
     misc_feature    128..155
                     /note="/%_Identity=100"
                     /note="/Motif=GAGCACATCTCGTTCGCTATTCAGGGATTG"
                     /note="/annotation_group=flank: 2,715 -> 2,744"
                     /note="/modified_by=thjac"
                     /label="5' flank"
     GG              160..170
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     Linker          173..220
                     /note="/vntifkey=21"
                     /modified_by="mamee"
                     /label="Linker"
     CDS             221..916
                     /vntifkey=4
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mScarlet"
                     /Transferred_Similarity="100.00%"
                     /Adjusted_Transferred_Similarity="96.67%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/-24
                     (adjusted to 96.67%; Adjusted-Interval-0:0/0); mScarlet-L
                     (<a href=""urn:local:lapau:r-e4ew2m2"">mScarlet</a>)"
                     /label="mScarlet-L"
     Linker          917..1006
                     /modified_by="mamee"
                     /label="Linker2"
     CDS             1007..1096
                     /note="/vntifkey=21"
                     /modified_by="mamee"
                     /label="HO-Tag3"
     attL3           complement(1104..1199)
     primer          complement(1257..1277)
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        1338..1388
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    1389..2198
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2345..2933
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     primer          3532..3552
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
       61 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt tcttgtacaa
      121 agtggatgca catctcgttc gctattcagg gattgttttg gtctcaggct ccggcggact
      181 cgagtccgga ctcagatctg gcagcggtgg aggcgatatc atggtgagca agggcgaggc
      241 agtgatcaag gagttcatgc ggttcaaggt gcacatggag ggctccatga acggccacga
      301 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      361 gaaggtgacc aagggtggcc ccctgccctt ctcctgggac atcctgtccc ctcagttcat
      421 gtacggctcc agggccttca ccaagcaccc cgccgacatc cccgactact ataagcagtc
      481 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgccgtgac
      541 cgtgacccag gacacctccc tggaggacgg caccctgatc tacaaggtga agctccgcgg
      601 caccaacttc cctcctgacg gccccgtaat gcagaagaag acaatgggct gggaagcgtc
      661 caccgagcgg ttgtaccccg aggacggcgt gctgaagggc gacattaaga tggccctgcg
      721 cctgaaggac ggcggccgct acctggcgga cttcaagacc acctacaagg ccaagaagcc
      781 cgtgcagatg cccggcgcct acaacgtcga ccgcaagttg gacatcacct cccacaacga
      841 ggactacacc gtggtggaac agtacgaacg ctccgagggc cgccactcca ccggcggcat
      901 ggacgagctg tacaagggcg gactcgagtc cggactcaga tctggcagcg gtggaggcga
      961 tatcggcgga ctcgagtccg gactcagatc tggcagcggt ggaggcggag aaatcgcaaa
     1021 gagtctgaaa gaaattgcaa aatccttgaa ggaaatagcg tggtcactga aagaaattgc
     1081 caagtccctc aaagggtaat aatcaacttt attatacaaa gttggcatta taaaaaagca
     1141 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     1201 gagctccatg gtagcgttaa cgcggccgcg atatccccta tagtgagtcg tattacatgg
     1261 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1321 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     1381 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     1441 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1501 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     1561 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     1621 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     1681 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     1741 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     1801 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     1861 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     1921 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     1981 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2041 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2101 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2161 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     2221 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     2281 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2341 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2401 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2461 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     2521 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2581 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2641 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2701 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     2761 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2821 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2881 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2941 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3001 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3061 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3121 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3181 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc
     3241 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg
     3301 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac
     3361 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     3421 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt
     3481 tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt tgtaaaacga
     3541 cggccagtct taagctcggg ccctgcagct ctagagctcg aattctacag gtcactaata
     3601 ccatctaagt agttgat
//
