LOCUS       pGG-D-mCherry-HOTag3-E        3594 bp    DNA     circular UNA 06-APR-2023
DEFINITION  .
ACCESSION   urn.local.wosma.21-fdze5tq
VERSION     urn.local.wosma.21-fdze5tq
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:89-dgycryy"">D</a>"
                     /label="D"
     misc_feature    <8..11
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        8..11
                     /label="Ligation"
     CDS             14..721
                     /gene="mCherry"
                     /codon_start=1
                     /transl_table=11
                     /product="mCherry"
                     /protein_id="AHE38513.1"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK"
                     /modified_by="mamee"
                     /label="mCherry"
     Linker          728..826
                     /created_by="wosma"
                     /modified_by="mamee"
                     /label="LinkerII"
     CDS             827..916
                     /region_type="Alpha-helix"
                     /modified_by="mamee"
                     /label="HOTag3"
     Stop            917..919
                     /created_by="mamee"
                     /label="STOP"
     GG              complement(920..930)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:82-dgycryx"">E</a>"
                     /label="E"
     ligation        920..923
                     /label="Ligation"
     primer          complement(950..970)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1450..1554
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1555..2415
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2586..3174
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3538..3559
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcatca gccatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
       61 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      121 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      181 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
      241 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
      301 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      361 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
      421 cgacggcccc gtaatgcaga agaagacgat gggctgggag gcctcctccg agcggatgta
      481 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
      541 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      601 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      661 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
      721 gtcagctgga tccggcagtg ctggtggttc tgcaggaggt tcagctggtg gaagtgccgg
      781 tggatctgct gggggtagtg ccggtggaag tgctggcggt tctagaggtg aaatcgccaa
      841 gagcttgaag gagattgcaa agagtcttaa agagatagct tggtctttga aagaaattgc
      901 taagtcatta aaaggatgac tgctgagacc gaattctcgc cctatagtga gtcgtattac
      961 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     1021 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     1081 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     1141 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     1201 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     1261 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     1321 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     1381 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1441 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1501 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1561 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1621 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1681 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1741 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     1801 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1861 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1921 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1981 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2041 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2101 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2161 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2221 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg ctctcgaggt
     2281 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2341 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2401 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2461 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2521 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2581 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2641 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2701 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2761 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2821 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2881 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2941 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3001 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3061 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3121 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3181 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3241 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3301 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3361 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3421 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     3481 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     3541 agcggataac aatttcacac gagctattta ggtgacacta tagaagtgaa gctt
//
