LOCUS       pGG-D-eGFP-HOTag3-E        3651 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local.wosma.23-fdze5tq
VERSION     urn.local.wosma.23-fdze5tq
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:89-dgycryy"">D</a>"
                     /label="D"
     misc_feature    <8..11
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     ligation        8..11
                     /label="Ligation"
     Linker          14..61
                     /region_type="Turn"
                     /modified_by="mamee"
                     /label="LinkerI"
     CDS             62..778
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="EGFP"
                     /Transferred_Similarity="99.72%"
                     /Primary_Match="99.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:hy-e4twm0o"">EGFP</a>"
                     /Alternative_Match="99.72%; Adjusted-Interval-0:0/0; Egfp
                     (<a href=""urn:local:.:fg-eaul7he"">8 Egfp</a>)"
                     /Alternative_Match="99.30%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:hh-e4tw8wz"">ECFP</a>"
                     /Alternative_Match="99.30%; Adjusted-Interval-0:0/0; Ecfp
                     (<a href=""urn:local:.:ff-eaul7he"">9 ECFP</a>)"
                     /Alternative_Match="98.88%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8y-dgycryy"">GFP</a>"
                     /Alternative_Match="98.33%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ie-e4twtil"">EYFP</a>"
                     /Alternative_Match="98.33%; Adjusted-Interval-0:0/0; Eyfp
                     (<a href=""urn:local:.:fh-eaul7he"">10 EYFP</a>)"
                     /label="EGFP"
     ligation        779..782
                     /label="Ligation"
     Linker          785..883
                     /created_by="wosma"
                     /modified_by="mamee"
                     /label="LinkerII"
     misc_feature    785..805
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     CDS             884..973
                     /region_type="Alpha-helix"
                     /modified_by="mamee"
                     /label="HOTag3"
     Stop            974..976
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="STOP"
     misc_feature    974..>980
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     GG              complement(977..987)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:82-dgycryx"">E</a>"
                     /label="E"
     ligation        977..980
                     /label="Ligation"
     primer          complement(1007..1027)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1507..1611
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1612..2472
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2643..3231
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3595..3616
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcatca gccggcggac tcgagtccgg actcagatct ggcagcggtg gaggcgatat
       61 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      121 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      181 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      241 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      301 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      361 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      421 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      481 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      541 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      601 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      661 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      721 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
      781 agctggatcc ggcagtgctg gtggttctgc aggaggttca gctggtggaa gtgccggtgg
      841 atctgctggg ggtagtgccg gtggaagtgc tggcggttct agaggtgaaa tcgccaagag
      901 cttgaaggag attgcaaaga gtcttaaaga gatagcttgg tctttgaaag aaattgctaa
      961 gtcattaaaa ggatgactgc tgagaccgaa ttctcgccct atagtgagtc gtattacctg
     1021 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     1081 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     1141 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     1201 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
     1261 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     1321 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1381 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     1441 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     1501 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1561 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1621 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1681 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1741 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1801 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1861 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1921 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1981 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2041 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2101 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2161 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2221 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2281 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggctc tcgaggtatc
     2341 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2401 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2461 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2521 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2581 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2641 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2701 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2761 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2821 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2881 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2941 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3001 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3061 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     3541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     3601 ggataacaat ttcacacgag ctatttaggt gacactatag aagtgaagct t
//
