LOCUS       pGG-E-STOP-RBS-mCherry-F        3425 bp    DNA     circular SYN 21-APR-2023
DEFINITION  .
ACCESSION   KF718993
VERSION     KF718993.1
KEYWORDS    .
SOURCE      Cloning vector pGGC015 (unknown)
  ORGANISM  Cloning vector pGGC015
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     <ahref=""urn:local:.:88-dgycryy"">C</a>"
                     /modified_by="mamee"
                     /label="E"
     misc_feature    7^8
                     /Original_Bases="GG"
                     /label="GG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    10..11
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    13
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     Stop            14..16
                     /cds_type="ORF"
                     /modified_by="mamee"
                     /label="Stop codon"
     CDS             17..25
                     /cds_type="ORF"
                     /label="Transcription enhancer"
     CDS             28..33
                     /cds_type="ORF"
                     /label="RBS"
     CDS             40..747
                     /created_by="User"
                     /modified_by="User"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     <ahref=""urn:local:.:93-dgycryy"">mCherry</a>"
                     /label="mCherry"
     Stop            748..750
                     /created_by="mamee"
                     /label="STOP"
     misc_feature    749..754
                     /Original_Bases="CAG"
                     /label="CAG"
                     /note="Geneious type: Editing History Replacement"
     GG              complement(751..761)
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     <ahref=""urn:local:.:81-dgycryx"">D</a>"
                     /modified_by="mamee"
                     /label="F"
     primer          complement(781..801)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%;
                     Adjusted-Interval-0:0/0;GG_SEQ_rev
                     (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1281..1385
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp
                     (<ahref=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1386..2246
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     <ahref=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2417..3005
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number
                     ColE1/pMB1/pBR322/pUCorigin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%;
                     Adjusted-Interval-0:0/0;ColE1/pMB1/pBR322/pUC Ori
                     (<ahref=""urn:local:.:8d-dk4mzop"">high-copy-numberColE1/p
                     MB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3369..3390
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;GG_SEQ_fw
                     (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcactg ccttagttaa ctttaagaag gagtacggca tggtgagcaa gggcgaggag
       61 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
      121 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
      181 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
      241 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
      301 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
      361 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
      421 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gacgatgggc
      481 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
      541 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
      601 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
      661 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
      721 accggcggca tggacgagct gtacaagtaa actatgagac cgaattctcg ccctatagtg
      781 agtcgtatta cctggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      841 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      901 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
      961 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     1021 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     1081 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     1141 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     1201 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     1261 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1321 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1381 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     1441 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1501 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1561 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1621 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1681 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1741 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1801 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1861 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1921 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1981 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2041 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2101 gctctcgagg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2161 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2221 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     2281 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     2341 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2401 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2461 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2521 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2581 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2641 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2701 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2761 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2821 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2881 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2941 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3001 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3061 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3121 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3181 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3241 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     3301 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     3361 gtggaattgt gagcggataa caatttcaca cgagctattt aggtgacact atagaagtga
     3421 agctt
//
