LOCUS       pGG-C-GUS-D(-AarI)        4497 bp    DNA     circular SYN 23-FEB-2023
DEFINITION  .
ACCESSION   KF718997
VERSION     KF718997.1
KEYWORDS    .
SOURCE      Cloning vector pGGC051
  ORGANISM  Cloning vector pGGC051
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     CDS             14..1822
                     /gene="gus"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="AHE38521.1"
                     /modified_by="mamee"
                     /Original_Translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWW
                     ESALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTH
                     YGKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITD
                     ENGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVAN
                     GDVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPL
                     RVGIRSVAVKGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANS
                     YRTSHYPYAEEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGE
                     TQQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPI
                     TCVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQP
                     IIITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATS
                     QGILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
                     /label="GUS"
     misc_feature    628
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     modified_base   628
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label
     misc_feature    1339
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     modified_base   1339
                     /created_by="mamee"
                     /label
     GG              complement(1823..1833)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:81-dgycryx"">D</a>"
                     /label="D"
     primer          complement(1853..1873)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2353..2457
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2458..3318
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3489..4077
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4441..4462
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggtcc gtcctgtaga aaccccaacc cgtgaaatca aaaaactcga
       61 cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc gttggtggga
      121 aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg atcagttcgc
      181 cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag tctttatacc
      241 gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc attacggcaa
      301 agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc catttgaagc
      361 cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt gtgtgaacaa
      421 cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa acggcaagaa
      481 aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca gcgtaatgct
      541 ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg tcgcgcaaga
      601 ctgtaaccac gcgtctgttg actggcaagt ggtggccaat ggtgatgtca gcgttgaact
      661 gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga ctttgcaagt
      721 ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt gcgtcacagc
      781 caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt cagtggcagt
      841 gaagggcgaa cagttcctga ttaaccacaa accgttctac tttactggct ttggtcgtca
      901 tgaagatgcg gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc acgaccacgc
      961 attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt acgctgaaga
     1021 gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg ctgctgtcgg
     1081 ctttaacctc tctttaggca ttggtttcga agcgggcaac aagccgaaag aactgtacag
     1141 cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta aagagctgat
     1201 agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg aaccggatac
     1261 ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg gaagcaacgc gtaaactcga
     1321 cccgacgcgt ccgatcacgt gcgtcaatgt aatgttctgc gacgctcaca ccgataccat
     1381 cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg tccaaagcgg
     1441 cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc aggagaaact
     1501 gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc tgcactcaat
     1561 gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt
     1621 ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg attttgcgac
     1681 ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc gcgaccgcaa
     1741 accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact tcggtgaaaa
     1801 accgcagcag ggaggcaaac aatcagtgag accgaattct cgccctatag tgagtcgtat
     1861 tacctggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1921 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1981 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     2041 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     2101 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2161 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2221 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     2281 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2341 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2401 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2461 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2521 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2581 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2641 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2701 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2761 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2821 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2881 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2941 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3001 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3061 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3121 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggctctcga
     3181 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3241 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3301 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3361 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3421 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3481 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3541 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3601 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3661 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3721 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3781 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3841 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3901 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3961 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4021 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4081 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4141 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4201 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4261 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     4321 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     4381 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     4441 gtgagcggat aacaatttca cacgagctat ttaggtgaca ctatagaagt gaagctt
//
