LOCUS       pGG-B-3xHA-TR-TUBE-Linker-C        3595 bp    DNA     circular SYN 13-MAR-2023
DEFINITION  .
ACCESSION   KF718965
VERSION     KF718965.1
KEYWORDS    .
SOURCE      Cloning vector pGGB000 (unknown)
  ORGANISM  Cloning vector pGGB000
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Lampropoulos,A., Sutikovic,Z., Wenzl,C., Maegele,I., Lohmann,J.U.
            and Forner,J.
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for
            Plant Transgenesis
  JOURNAL   PLoS ONE 8 (12), E83043 (2013)
  PUBMED    24376629
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Forner,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2013) Centre for Organismal Studies,
            Ruprecht-Karls-Universitaet Heidelberg, Im Neuenheimer Feld 230,
            Heidelberg D-69221, Germany
COMMENT     ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy    
            sequencing ##Assembly-Data-END##.
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:87-dgycryy"">B</a>"
                     /label="B"
     tag             12..104
                     /modified_by="mamee"
                     /label="3xHA"
     misc_feature    16..104
                     /Original_Bases="ACTACAAGGACGACGATGACAAAGTCAAGCTCTACCCATAC
                     GATGTTCCAGATTACGCTGGATCCGAATTC"
                     /label="ACTACAAGGACGACGATGACAAAGTCAAGCTCTACCCATACGATGTTCCA
                     GATTACGCTGGATCCGAATTC"
                     /note="Geneious type: Editing History Replacement"
     CDS             105..872
                     /modified_by="mamee"
                     /label="TR-TUBE"
     CDS             120..305
                     /created_by="mamee"
                     /modified_by="mamee"
                     /label="Repeat 1"
     CDS             306..491
                     /created_by="mamee"
                     /label="Repeat 2"
     CDS             492..677
                     /created_by="mamee"
                     /label="Repeat 3"
     CDS             678..863
                     /created_by="mamee"
                     /label="Repeat 4"
     misc_feature    873..920
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     Linker          873..920
                     /created_by="wacae"
                     /modified_by="mamee"
     GG              complement(921..931)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:80-dgycryx"">C</a>"
                     /label="C"
     primer          complement(951..971)
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     primer_bind     complement(1076..1095)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="1046038988791000af76b82-b126-4043-b1c1-
                     a55a04d13139"
                     /created_by="primer3"
                     /Sequence="AACTGTTGGGAAGGGCGATC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pGG-B-3xHA-TR-TUBE-Linker-C"
                     /label="MAMEE242"
     promoter        1451..1555
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1556..2416
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2587..3175
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     3387..3406
                     /%GC="50.0"
                     /Hairpin_Tm="39.6"
                     /Self_Dimer_Tm="None"
                     /Tm="59.6"
                     /annotation_group="104603353447500c688c0c1-068e-408f-8b56-
                     68a46e22a73a"
                     /created_by="primer3"
                     /Sequence="CGCGTTGGCCGATTCATTAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pGG-B-3xHA-TR-TUBE-Linker-C"
                     /label="MAMEE156"
     primer          3539..3560
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatggcatac ccttacgatg ttcctgacta tgcgggctat ccctatgacg
       61 tcccggacta tgccggctac ccttacgacg ttccagatta cgctatgggg atcctcagat
      121 ctggaggtgg agtaaatcct cagctacaga atccagaagt cgcgtttcag caacaactgg
      181 aacaactcag tgcaatggga tttttgaacg cggaagcaaa cttgcaagct ctaatagcaa
      241 caggaggtga tattaatgca gctattgaag cgttactggg ctcccagcca tcaggaggtg
      301 gaggatctgg aggtggagta aatcctcagc tacagaatcc agaagtcgcg tttcagcaac
      361 aactggaaca actcagtgca atgggatttt tgaacgcgga agcaaacttg caagctctaa
      421 tagcaacagg aggtgatatt aatgcagcta ttgaagcgtt actgggctcc cagccatcag
      481 gaggtggagg atctggaggt ggagtaaatc ctcagctaca gaatccagaa gtcgcgtttc
      541 agcaacaact ggaacaactc agtgcaatgg gatttttgaa cgcggaagca aacttgcaag
      601 ctctaatagc aacaggaggt gatattaatg cagctattga agcgttactg ggctcccagc
      661 catcaggagg tggaggatct ggaggtggag taaatcctca gctacagaat ccagaagtcg
      721 cgtttcagca acaactggaa caactcagtg caatgggatt tttgaacgcg gaagcaaact
      781 tgcaagctct aatagcaaca ggaggtgata ttaatgcagc tattgaagcg ttactgggct
      841 cccagccatc aggaggtgga ggatcgatcc ccggttctgg ttcaggttca ggttctggat
      901 ctggaagtgg atctggatca ggcttgagac cgaattctcg ccctatagtg agtcgtatta
      961 cctggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     1021 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     1081 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     1141 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     1201 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     1261 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     1321 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     1381 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     1441 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1501 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1561 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     1621 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     1681 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     1741 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     1801 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     1861 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     1921 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     1981 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2041 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     2101 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2161 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2221 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gctctcgagg
     2281 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2341 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2401 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2461 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2521 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     2581 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2641 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2701 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2761 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     2821 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2881 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2941 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3001 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3061 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3121 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3181 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3241 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3301 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     3361 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     3421 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     3481 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     3541 gagcggataa caatttcaca cgagctattt aggtgacact atagaagtga agctt
//
