LOCUS       35SP-mCherry-N7-NOST-ZmUBIP-AtCas9-Bpstar-G7T       11093 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local.jonas.35-fkpmr80
VERSION     urn.local.jonas.35-fkpmr80
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..864
                     /regulatory_class="promoter"
                     /gene="CaMV 35S"
                     /note="internal BsaI recognition site removed"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S - Golden Gate"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:6v-dgycryx"">pCaMV 35S - Golden
                     Gate</a>)"
                     /label="p35S"
     Linker          869..892
                     /created_by="wacae"
     CDS             899..1606
                     /label="mCherry"
     NLS             1613..1909
                     /codon_start=1
                     /transl_table=11
                     /product="linker-NLS"
                     /protein_id="AHE38530.1"
                     /translation="SGSGSGSGSGSGSGSAAASEFKREEQARKAKVNNEKKTEIVKPE
                     SCSNEGDVKDLKRKDSEDGNEGEEEEASSKPKKPKVALSHLQDIDDTEADQEEE"
                     /modified_by="mamee"
                     /label="linker-NLS"
     terminator      1914..2181
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /label="NOST"
     promoter        2210..4195
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/1;
                     ZmUBIL.2 (<a
                     href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     intron          3187..4196
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     Linker          4200..4223
                     /created_by="wacae"
     CDS             4230..8333
                     /Transferred_From="Cas9PTA"
                     /Transferred_Similarity="100.00%"
                     /label="CAS9"
     NLS             8340..8390
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="BP*"
     terminator      8398..8669
                     /label="G7T"
     Linker          8674..8722
                     /created_by="wacae"
                     /Color="#ff9911"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6k-dgycryx"">Linker II</a>"
                     /label="Linker II"
     primer          complement(8783..8803)
                     /vntifkey=27
                     /label="GWM13R"
     promoter        8864..8914
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    8915..9724
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      9871..10459
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          11058..11078
                     /vntifkey=27
                     /label="GWM13F"
ORIGIN      
        1 ccgatccatt cggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
       61 tggttagaga ggcttacgca gcacgtctca tcaagacgat ctacccgagc aataatctcc
      121 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
      181 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
      241 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
      301 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
      361 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
      421 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
      481 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
      541 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
      601 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
      661 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      721 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      781 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      841 tggagagaac acgggggacg agctaacagt attcagtcga ctggtaccaa caggctccat
      901 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      961 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1021 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1081 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1141 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1201 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     1261 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1321 gcagaagaag acgatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1381 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1441 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1501 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     1561 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtcag gttctggttc
     1621 aggttcaggt tctggatctg gaagtggatc tggatcagcg gccgcatctg aattcaagcg
     1681 tgaagagcaa gcaaggaaag ctaaggtgaa caatgagaaa aagacggaaa tagtgaaacc
     1741 agagagttgt agcaatgaag gagatgtcaa ggatctgaaa agaaaggact ctgaggatgg
     1801 aaacgagggt gaggaagaag aagcttcttc gaaaccgaaa aagccaaaag ttgctctttc
     1861 tcatcttcag gacattgacg acacagaagc tgatcaagaa gaagagtaac tgcctagagt
     1921 caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     1981 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     2041 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     2101 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     2161 tcatctatgt tactagatcg aactataagc taggtagtaa ctagtacctg tgcagcgtga
     2221 cccggtcgtg cccctctcta gagataatga gcattgcatg tctaagttat aaaaaattac
     2281 cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta tacatatatt
     2341 taaactttac tctacgaata atataatcta tagtactaca ataatatcag tgttttagag
     2401 aatcatataa atgaacagtt agacatggtc taaaggacaa ttgagtattt tgacaacagg
     2461 actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc aaatagcttc
     2521 acctatataa tacttcatcc attttattag tacatccatt tagggtttag ggttaatggt
     2581 ttttatagac taattttttt agtacatcta ttttattcta ttttagcctc taaattaaga
     2641 aaactaaaac tctattttag tttttttatt taataattta gatataaaat agaataaaat
     2701 aaagtgacta aaaattaaac aaataccctt taagaaatta aaaaaactaa ggaaacattt
     2761 ttcttgtttc gagtagataa tgccagcctg ttaaacgccg tcgacgagtc taacggacac
     2821 caaccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg
     2881 tcgctgcctc tggacccctc tcgagagttc cgctccaccg ttggacttgc tccgctgtcg
     2941 gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg cggcctcctc
     3001 ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct
     3061 tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt
     3121 gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc
     3181 ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt
     3241 ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt
     3301 ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt
     3361 tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc
     3421 cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct
     3481 tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt
     3541 tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt
     3601 ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata
     3661 ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt
     3721 tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat
     3781 gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact
     3841 acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg
     3901 agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac
     3961 tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct
     4021 atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat
     4081 ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg
     4141 cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt ctgcaaacag
     4201 tattcagtcg actggtacca acaggctcga tggataagaa gtactctatc ggactcgata
     4261 tcggaactaa ctctgtggga tgggctgtga tcaccgatga gtacaaggtg ccatctaaga
     4321 agttcaaggt tctcggaaac accgataggc actctatcaa gaaaaacctt atcggtgctc
     4381 tcctcttcga ttctggtgaa actgctgagg ctaccagact caagagaacc gctagaagaa
     4441 ggtacaccag aagaaagaac aggatctgct acctccaaga gatcttctct aacgagatgg
     4501 ctaaagtgga tgattcattc ttccacaggc tcgaagagtc attcctcgtg gaagaagata
     4561 agaagcacga gaggcaccct atcttcggaa acatcgttga tgaggtggca taccacgaga
     4621 agtaccctac tatctaccac ctcagaaaga agctcgttga ttctactgat aaggctgatc
     4681 tcaggctcat ctacctcgct ctcgctcaca tgatcaagtt cagaggacac ttcctcatcg
     4741 agggtgatct caaccctgat aactctgatg tggataagtt gttcatccag ctcgtgcaga
     4801 cctacaacca gcttttcgaa gagaacccta tcaacgcttc aggtgtggat gctaaggcta
     4861 tcctctctgc taggctctct aagtcaagaa ggcttgagaa cctcattgct cagctccctg
     4921 gtgagaagaa gaacggactt ttcggaaact tgatcgctct ctctctcgga ctcaccccta
     4981 acttcaagtc taacttcgat ctcgctgagg atgcaaagct ccagctctca aaggatacct
     5041 acgatgatga tctcgataac ctcctcgctc agatcggaga tcagtacgct gatttgttcc
     5101 tcgctgctaa gaacctctct gatgctatcc tcctcagtga tatcctcaga gtgaacaccg
     5161 agatcaccaa ggctccactc tcagcttcta tgatcaagag atacgatgag caccaccagg
     5221 atctcacact tctcaaggct cttgttagac agcagctccc agagaagtac aaagagattt
     5281 tcttcgatca gtctaagaac ggatacgctg gttacatcga tggtggtgca tctcaagaag
     5341 agttctacaa gttcatcaag cctatcctcg agaagatgga tggaaccgag gaactcctcg
     5401 tgaagctcaa tagagaggat cttctcagaa agcagaggac cttcgataac ggatctatcc
     5461 ctcatcagat ccacctcgga gagttgcacg ctatccttag aaggcaagag gatttctacc
     5521 cattcctcaa ggataacagg gaaaagattg agaagattct caccttcaga atcccttact
     5581 acgtgggacc tctcgctaga ggaaactcaa gattcgcttg gatgaccaga aagtctgagg
     5641 aaaccatcac cccttggaac ttcgaagagg tggtggataa gggtgctagt gctcagtctt
     5701 tcatcgagag gatgaccaac ttcgataaga accttccaaa cgagaaggtg ctccctaagc
     5761 actctttgct ctacgagtac ttcaccgtgt acaacgagtt gaccaaggtt aagtacgtga
     5821 ccgagggaat gaggaagcct gcttttttgt caggtgagca aaagaaggct atcgttgatc
     5881 tcttgttcaa gaccaacaga aaggtgaccg tgaagcagct caaagaggat tacttcaaga
     5941 aaatcgagtg cttcgattca gttgagattt ctggtgttga ggataggttc aacgcatctc
     6001 tcggaaccta ccacgatctc ctcaagatca ttaaggataa ggatttcttg gataacgagg
     6061 aaaacgagga tatcttggag gatatcgttc ttaccctcac cctctttgaa gatagagaga
     6121 tgattgaaga aaggctcaag acctacgctc atctcttcga tgataaggtg atgaagcagt
     6181 tgaagagaag aagatacact ggttggggaa ggctctcaag aaagctcatt aacggaatca
     6241 gggataagca gtctggaaag acaatccttg atttcctcaa gtctgatgga ttcgctaaca
     6301 gaaacttcat gcagctcatc cacgatgatt ctctcacctt taaagaggat atccagaagg
     6361 ctcaggtttc aggacagggt gatagtctcc atgagcatat cgctaacctc gctggatctc
     6421 ctgcaatcaa gaagggaatc ctccagactg tgaaggttgt ggatgagttg gtgaaggtga
     6481 tgggaaggca taagcctgag aacatcgtga tcgaaatggc tagagagaac cagaccactc
     6541 agaagggaca gaagaactct agggaaagga tgaagaggat cgaggaaggt atcaaagagc
     6601 ttggatctca gatcctcaaa gagcaccctg ttgagaacac tcagctccag aatgagaagc
     6661 tctacctcta ctacctccag aacggaaggg atatgtatgt ggatcaagag ttggatatca
     6721 acaggctctc tgattacgat gttgatcata tcgtgccaca gtcattcttg aaggatgatt
     6781 ctatcgataa caaggtgctc accaggtctg ataagaacag gggtaagagt gataacgtgc
     6841 caagtgaaga ggttgtgaag aaaatgaaga actattggag gcagctcctc aacgctaagc
     6901 tcatcactca gagaaagttc gataacttga ctaaggctga gaggggagga ctctctgaat
     6961 tggataaggc aggattcatc aagaggcagc ttgtggaaac caggcagatc actaagcacg
     7021 ttgcacagat cctcgattct aggatgaaca ccaagtacga tgagaacgat aagttgatca
     7081 gggaagtgaa ggttatcacc ctcaagtcaa agctcgtgtc tgatttcaga aaggatttcc
     7141 aattctacaa ggtgagggaa atcaacaact accaccacgc tcacgatgct taccttaacg
     7201 ctgttgttgg aaccgctctc atcaagaagt atcctaagct cgagtcagag ttcgtgtacg
     7261 gtgattacaa ggtgtacgat gtgaggaaga tgatcgctaa gtctgagcaa gagatcggaa
     7321 aggctaccgc taagtatttc ttctactcta acatcatgaa tttcttcaag accgagatta
     7381 ccctcgctaa cggtgagatc agaaagaggc cactcatcga gacaaacggt gaaacaggtg
     7441 agatcgtgtg ggataaggga agggatttcg ctaccgttag aaaggtgctc tctatgccac
     7501 aggtgaacat cgttaagaaa accgaggtgc agaccggtgg attctctaaa gagtctatcc
     7561 tccctaagag gaactctgat aagctcattg ctaggaagaa ggattgggac cctaagaaat
     7621 acggtggttt cgattctcct accgtggctt actctgttct cgttgtggct aaggttgaga
     7681 agggaaagag taagaagctc aagtctgtta aggaacttct cggaatcact atcatggaaa
     7741 ggtcatcttt cgagaagaac ccaatcgatt tcctcgaggc taagggatac aaagaggtta
     7801 agaaggatct catcatcaag ctcccaaagt actcactctt cgaactcgag aacggtagaa
     7861 agaggatgct cgcttctgct ggtgagcttc aaaagggaaa cgagcttgct ctcccatcta
     7921 agtacgttaa ctttctttac ctcgcttctc actacgagaa gttgaaggga tctccagaag
     7981 ataacgagca gaagcaactt ttcgttgagc agcacaagca ctacttggat gagatcatcg
     8041 agcagatctc tgagttctct aaaagggtga tcctcgctga tgcaaacctc gataaggtgt
     8101 tgtctgctta caacaagcac agagataagc ctatcaggga acaggcagag aacatcatcc
     8161 atctcttcac ccttaccaac ctcggtgctc ctgctgcttt caagtacttc gatacaacca
     8221 tcgataggaa gagatacacc tctaccaaag aagtgctcga tgctaccctc atccatcagt
     8281 ctatcactgg actctacgag actaggatcg atctctcaca gctcggtggt gattcaggta
     8341 aaagaaccgc cgacggcagc gaattcgagc ctaagaagaa gaggaaggtt tgactgcccc
     8401 cgatgagcta agctagctat atcatcaatt tatgtattac acataatatc gcactcagtc
     8461 tttcatctac ggcaatgtac cagctgatat aatcagttat tgaaatattt ctgaatttaa
     8521 acttgcatca ataaatttat gtttttgctt ggactataat acctgacttg ttattttatc
     8581 aataaatatt taaactatat ttctttcaag atgggaatta acatctacaa attgcctttt
     8641 cttatcgacc atgtactcga cggccgagta ctataagcta ggtagtaact agtgcgactg
     8701 tctagaatcg cgtatgcgag tgtatctaga atcgcgtatg cgagtgtatg agctcgatat
     8761 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     8821 tcaaaatctc tgatgttaca ttgcacaaga taaaataata tcatcatgaa caataaaact
     8881 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     8941 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     9001 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     9061 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     9121 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     9181 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     9241 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     9301 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     9361 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     9421 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     9481 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     9541 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     9601 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     9661 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     9721 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     9781 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     9841 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9901 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9961 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    10021 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10081 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
    10141 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
    10201 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
    10261 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
    10321 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
    10381 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
    10441 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
    10501 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
    10561 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
    10621 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
    10681 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    10741 cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc
    10801 gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc
    10861 caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc
    10921 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagacttc cgactgagcc
    10981 tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt
    11041 ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggccca cct
//
