LOCUS       pBGWD,0                11334 bp    DNA     circular     23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...5d-dntgqzh
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|434996218|                                                 
            VNTDBDATE|458479736|                                               
            LSOWNER|                                                           
            VNTNAME|pBGWD,0|                                                   
            VNTAUTHORNAME|Demo User|                                           
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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                          (LOGFONT 47 17 0 0 400 255 1 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Cla}I (3255)" "@N (@S)" 5 14.1102         
                                  -3.47421 0 -10 2.83835 0.694805 #234#))      
                              (CObjectList))                                   
                        #236=(CGroupWidget                                     
                              (CWidget 2 (10 #1# 0) 1 2 0 0 Nil -116 100)      
                              (CObjectList                                     
                               #237=(CScratch                                  
                                     (CWidget 1 (1 #1# 1) 1 2 0 0 #151#
            47254152                                 100) (LOGPEN 0 10 0) 8 1
            1.90238 1.9                                0.123113 1)             
                                                 #238=(CLabel                  
                                                       (CWidget 0 (0 0) 1 2 0 0
            #179# 30113308 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 47
            17 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Apa}I (7877)" "@N (@S)" 5 -13.9333                           
                -4.63228 0 -10 2.92705 0.694805 #237#)                         
            #239=(CScratch                                                     
                  (CWidget 2 (1 #1# 2) 1 2 0 0 #151# 30185736                  
                          100) (LOGPEN 0 10 0) 8 1 0.0643745 1.9               
                            0.123113 1)                                        
                      #240=(CLabel                                             
                            (CWidget 0 (0 0) 1 2 0 0 #179# 1004 100)           
                                (LOGPEN 0 0 8437824) 1                         
                                (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34      
                                      "Arial") 1.15807 0.611111 0              
                                     "{\\i Apa}I (11189)" "@N (@S)" 5 -3.60498 
                                          19.6872 0 -10 3.22271 0.694805
            #239#))                         (CObjectList))                     
                                      #241=(CGroupWidget                       
                                            (CWidget 2 (10 #6# 0) 1 2 0 0 Nil
            -273 100)                         (CObjectList                     
                                               #242=(CScratch                  
                                                     (CWidget 1 (1 #6# 1) 1 2 0
            0 #151# 28797928                                 100) (LOGPEN 0 10
            0) 8 1 1.63212 1.9                                0.123113 1)      
                                                        #243=(CLabel           
                                                              (CWidget 0 (0 0)
            1 2 0 0 #179# 268697602 100)                                (LOGPEN
            0 0 8437824) 1                                             
            (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34                          
                  "Arial") 1.15807 0.611111 0                                  
                 "{\\i Eco}RI (8364)" "@N (@S)" 5 -14.7034 0 0                 
                          -10 3.31141 0.694805 #242#)                          
                    #244=(CScratch                                             
                          (CWidget 2 (1 #6# 2) 1 2 0 0 #151# 47252072          
                                  100) (LOGPEN 0 10 0) 8 1 0.241959 1.9        
                                   0.123113 1)                                 
                             #245=(CLabel                                      
                                   (CWidget 0 (0 0) 1 2 0 0 #179# 268697602
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 47 17 0 0 400 255 0 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Eco}RI
            (10869)" "@N (@S)" 5 -5.89811                               
            15.0549 0 -10 3.60707 0.694805 #244#))                        
            (CObjectList))                                               
            #246=(CGroupWidget                                                 
                  (CWidget 2 (10 #7# 0) 1 2 0 0 Nil -1683 100)                 
                   (CObjectList                                                
                    #247=(CScratch                                             
                          (CWidget 1 (1 #7# 1) 1 2 0 0 #151# 30887464          
                                  100) (LOGPEN 0 10 0) 8 1 1.88184 1.9         
                                  0.123113 1)                                  
                            #248=(CLabel                                       
                                  (CWidget 0 (0 0) 1 2 0 0 #179# 30272460 100) 
                                          (LOGPEN 0 0 8437824) 1               
                                          (LOGFONT 47 17 0 0 400 255 0 0 0 3 2
            1 34                                 "Arial") 1.15807 0.611111 0   
                                                "{\\i Eco}RV (7914)" "@N (@S)"
            5 -14.6482                                -2.31614 0 -10 3.51837
            0.694805 #247#)                          #249=(CScratch            
                                                           (CWidget 2 (1 #7# 2)
            1 2 0 0 #151# 47247992                                 100) (LOGPEN
            0 10 0) 8 1 0.932321 1.9                                0.123113 1)
                                                              #250=(CLabel
            (CWidget 0 (0 0) 1 2 0 0 #179# 0 100)                              
             (LOGPEN 0 0 8437824) 1                                            
             (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34                         
                   "Arial") 1.15807 0.611111 0                                 
                  "{\\i Eco}RV (9625)" "@N (@S)" 5 -13.6704                    
                       5.79035 0 -10 3.51837 0.694805 #249#))                  
                  (CObjectList))                                               
            #251=(CGroupWidget                                                 
                  (CWidget 2 (10 #8# 0) 1 2 0 0 Nil -2234 100)                 
                   (CObjectList                                                
                    #252=(CScratch                                             
                          (CWidget 1 (1 #8# 1) 1 2 0 0 #151# 47253112          
                                  100) (LOGPEN 0 10 0) 8 1 6.27319 1.9         
                                  0.123113 1)                                  
                            #253=(CLabel                                       
                                  (CWidget 0 (0 0) 1 2 0 0 #179# 31905308 100) 
                                          (LOGPEN 0 0 8437824) 1               
                                          (LOGFONT 47 17 0 0 400 255 0 0 0 3 2
            1 34                                 "Arial") 1.15807 0.611111 0   
                                                "{\\i Hin}dIII (1)" "@N (@S)" 5
            4.40195                                12.7388 0 -10 2.54269
            0.694805 #252#)                          #254=(CScratch            
                                                           (CWidget 2 (1 #8# 2)
            1 2 0 0 #151# 31105560                                 100) (LOGPEN
            0 10 0) 8 1 0.907348 1.9                                0.123113 1)
                                                              #255=(CLabel     
                                                                    (CWidget 0
            (0 0) 1 2 0 0 #179# 31911332 100)                               
            (LOGPEN 0 0 8437824) 1                                             
            (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34                          
                  "Arial") 1.15807 0.611111 0                                  
                 "{\\i Hin}dIII (9670)" "@N (@S)" 5 -12.4532                   
                        9.26456 0 -10 3.42967 0.694805 #254#))                 
                   (CObjectList))                                              
             #256=(CGroupWidget                                                
                   (CWidget 2 (10 #14# 0) 1 2 0 0 Nil -668 100)                
                    (CObjectList                                               
                     #257=(CScratch                                            
                           (CWidget 1 (1 #14# 1) 1 2 0 0 #151# 30399984        
                                    100) (LOGPEN 0 10 0) 8 1 0.908458 1.9      
                                     0.123113 1)                               
                               #258=(CLabel                                    
                                     (CWidget 0 (0 0) 1 2 0 0 #179# 31770772
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 47 17 0 0 400 255 0 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Sac}I
            (9668)" "@N (@S)" 5 -12.1445                                8.10649
            0 -10 2.92705 0.694805 #257#)                         
            #259=(CScratch                                                     
                  (CWidget 2 (1 #14# 2) 1 2 0 0 #151# 47671152                 
                           100) (LOGPEN 0 10 0) 8 1 0.23641 1.9                
                           0.123113 1)                                         
                     #260=(CLabel                                              
                           (CWidget 0 (0 0) 1 2 0 0 #179# 268697602 100)       
                                    (LOGPEN 0 0 8437824) 1                     
                                    (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34  
                                          "Arial") 1.15807 0.611111 0          
                                         "{\\i Sac}I (10879)" "@N (@S)" 5
            -5.67437                                16.213 0 -10 3.22271
            0.694805 #259#))                         (CObjectList))            
                                               #261=(CGroupWidget              
                                                     (CWidget 2 (10 #18# 0) 1 2
            0 0 Nil -1138 100)                         (CObjectList            
                                                        #262=(CScratch         
                                                              (CWidget 1 (1
            #18# 1) 1 2 0 0 #151# 30230392                                 100)
            (LOGPEN 0 10 0) 8 1 0.930101 1.9                               
            0.123113 1)                                                  
            #263=(CLabel (CWidget 0 (0 0) 1 2 0 0 #179# 0 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Spe}I (9629)" "@N (@S)" 5 -12.7738        
                                   6.94842 0 -10 2.92705 0.694805 #262#)       
                              #264=(CScratch                                   
                                    (CWidget 2 (1 #18# 2) 1 2 0 0 #151#
            47258056                                 100) (LOGPEN 0 10 0) 8 1
            0.776934 1.9                                0.123113 1)            
                                                  #265=(CLabel (CWidget 0 (0 0)
            1 2 0 0 #179# 0 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 47
            17 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Spe}I (9905)" "@N (@S)" 5 -11.3664                           
                12.7388 0 -10 2.92705 0.694805 #264#))                        
            (CObjectList))                                               
            #266=(CGroupWidget                                                 
                  (CWidget 2 (10 #20# 0) 1 2 0 0 Nil -1529 100)                
                    (CObjectList                                               
                     #267=(CScratch                                            
                           (CWidget 1 (1 #20# 1) 1 2 0 0 #151# 47247512        
                                    100) (LOGPEN 0 10 0) 8 1 1.90793 1.9       
                                    0.123113 1)                                
                              #268=(CLabel (CWidget 0 (0 0) 1 2 0 0 #179# 0
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 47 17 0 0 400 255 0 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Xba}I
            (7867)" "@N (@S)" 5 -13.983                                -5.79035
            0 -10 2.98618 0.694805 #267#)                         
            #269=(CScratch                                                     
                  (CWidget 2 (1 #20# 2) 1 2 0 0 #151#                          
                   1195459412 100) (LOGPEN 0 10 0) 8 1 0.780263                
                           1.9 0.123113 1)                                     
                     #270=(CLabel (CWidget 0 (0 0) 1 2 0 0 #179# 0 100)        
                                   (LOGPEN 0 0 8437824) 1                      
                                   (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34   
                                         "Arial") 1.15807 0.611111 0           
                                        "{\\i Xba}I (9899)" "@N (@S)" 5
            -11.3138                                11.5807 0 -10 2.98618
            0.694805 #269#))                         (CObjectList)))
            (CObjectList))                         #271=(CGroupWidget (CWidget
            14 (16 0) 1 2 0 0 Nil -746 100)                  (CObjectList)
            (CObjectList))                                  #272=(CGroupWidget
            (CWidget 12 (0 0) 1 2 0 0 Nil -198 100)                 
            (CObjectList) (CObjectList))) (CObjectList)))         (CSeqView 10
            5 (CObjectList) (CObList) 1 (CObList)) (CObList) 0    
            (CStringList) 0 0 (CObList))).
FEATURES             Location/Qualifiers
     T-DNA_border    1..333
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     70..89
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="59861613671771008163aa81-09fc-492f-b066
                     -d7bc3fcc2978"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P8"
     terminator      complement(354..621)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     Plant_Selection complement(354..1472)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1k-e500sax"">Basta</a>"
                     /label="Basta"
     misc_feature    370^371
                     /Original_Bases="TAG"
                     /label="TAG"
                     /note="Geneious type: Editing History Deletion"
     CDS             complement(628..1179)
                     /%_Identity=100
                     /Motif="ATGAGCCCAGAACGACGCCCGGCCGACATCCGCCGTGCCACCGAGGCGGA
                     CATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAGACAAGCACGGTCAACTTC
                     CGTACCGAGCCGCAGGAACCGCAGGAGTGGACGGACGACCTCGTCCGTCTGCGGGAGC
                     GCTATCCCTGGCTCGTCGCCGAGGTGGACGGCGAGGTCGCCGGCATCGCCTACGCGGG
                     TCCCTGGAAGGCACGCAACGCCTACGACTGGACGGCCGAGTCGACCGTGTACGTCTCC
                     CCCCGCCACCAGCGGACGGGACTGGGCTCCACGCTCTACACCCACCTGCTGAAGTCCC
                     TGGAGGCACAGGGCTTCAAGAGCGTGGTCGCTGTCATCGGGCTGCCCAACGACCCGAG
                     CGTGCGCATGCACGAGGCGCTCGGATATGCCCCCCGCGGCATGCTGCGGGCGGCCGGC
                     TTCAAGCACGGGAACTGGCATGACGTGGGTTTCTGGCAGCTGGACTTCAGCCTGCCGG
                     TGCCGCCCCGTCCGGTCCTGCCCGTCACCGAAATCTGA"
                     /annotation_group="PAT: 8,732 -> 9,283"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PAT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:98-dgycryy"">PAT</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; PAT
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /label="PAT"
     primer_bind     845..864
                     /%GC="55.0"
                     /Hairpin_Tm="45.2"
                     /Self_Dimer_Tm="None"
                     /Tm="59.0"
                     /annotation_group="598616142574790023226f06-a495-473e-bb6b
                     -03debe14a509"
                     /created_by="primer3"
                     /Sequence="TTCAGCAGGTGGGTGTAGAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P9"
     promoter        complement(1200..1472)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     misc_feature    1473..1477
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1481..1483
                     /Original_Bases="TAT"
                     /label="TAT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1493
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1495^1496
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     attR1           1560..1684
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c-f0o2y6y"">attR1</a>"
     promoter        1709..1739
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    1763
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1793..2452
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     1948..1967
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.8"
                     /annotation_group="5986161483315900ca67af52-1421-4431-aa88
                     -f5d70791a9ae"
                     /created_by="primer3"
                     /Sequence="GCACAAGTTTTATCCGGCCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P12"
     primer_bind     complement(2023..2042)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5986161689647000254890df-1c02-4a4b-9e85
                     -17cbd741822d"
                     /created_by="primer3"
                     /Sequence="TCACCAGCTCACCGTCTTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P16"
     misc_feature    2377
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     UTR             2475..2793
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    2567
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     2731..2750
                     /%GC="55.0"
                     /Hairpin_Tm="35.1"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="59861615418796005f83bd98-990e-40da-a006
                     -7424c727a424"
                     /created_by="primer3"
                     /Sequence="CTGAGGTCGCCCGGTTTATT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P13"
     misc_feature    2785
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     CDS             2794..3099
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     attR2           complement(3140..3264)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     primer_bind     3322..3341
                     /%GC="55.0"
                     /Hairpin_Tm="44.4"
                     /Self_Dimer_Tm="32.8"
                     /Tm="60.6"
                     /annotation_group="5986161746626100eb102a03-6847-4165-88d8
                     -3f2300373fbb"
                     /created_by="primer3"
                     /Sequence="GCCTGCAGGTCACTGGATTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P17"
     terminator      complement(3328..3553)
                     /created_by="mamee"
                     /label="t35S"
     misc_feature    3461
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     CDS             complement(3582..4379)
                     /label="EgfpER"
     primer_bind     3999..4018
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="59861619822163000e221a63-662c-4f07-bf67
                     -b52445898f97"
                     /created_by="primer3"
                     /Sequence="GCCGTCGTCCTTGAAGAAGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P35"
     misc_feature    4352
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4417
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     promoter        complement(4533..4910)
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pROLD - Gateway"
                     /Transferred_Similarity="97.88%"
                     /Primary_Match="97.88%; Adjusted-Interval-0:0/0; pROLD (<a
                     href=""urn:local:.:uf-f190xjm"">pROLD - Gateway</a>)"
                     /label="pROLD"
     misc_feature    4542..4549
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    4911
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4913
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4915
                     /Original_Bases="AGAGTCG"
                     /label="AGAGTCG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4917..4919
                     /Original_Bases="CCT"
                     /label="CCT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4921..4922
                     /Original_Bases="CA"
                     /label="CA"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    4934
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     T-DNA_border    4950..5180
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     primer_bind     4971..4990
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="59861615741317005ef147d4-54f9-47c2-ad80
                     -194d544a4153"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P14"
     misc_feature    5065
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    5158..5187
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     complement(5233..5252)
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /Sequence="TTTCAGAAGACGGCTGCACT"
                     /Tm="59.9"
                     /%GC="50.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P37"
     primer_bind     5799..5817
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="5986160964932900aceeac81-9192-4c81-928e
                     -6520862ca693"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P1"
     regulatory      6366..6995
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     6591..6610
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5986161022635600ddf12b34-b6fe-4109-949f
                     -929c732ffd3a"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P2"
     primer_bind     7388..7406
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="5986161079399700fbba90a6-b619-4ffb-9ef5
                     -cb20824779f2"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P3"
     regulatory      7424..8497
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     misc_feature    7428
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     8192..8209
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="5986161138006400d7da41ce-a690-47c0-ad2b
                     -6e5d7f857e11"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P4"
     rep_origin      8563..8757
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     primer_bind     8992..9011
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="598616119554890093325b55-68da-49f6-b746
                     -7f5bfbaa4196"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P5"
     regulatory      9131..9391
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /label="pBR322 bom"
     rep_origin      complement(9427..10015)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     9816..9835
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="59861612523315000c1ad778-936d-4bf9-ba26
                     -9df8d12ba336"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P6"
     misc_feature    10201^10202
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    10213^10214
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    10218..10221
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(10261..11052)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     10588..10607
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="59861613093424000679ba29-14e1-43b8-9130
                     -beab3d764641"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGWD,0"
                     /label="P7"
     Promoter        complement(11053..11327)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    11187^11188
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    11198^11199
                     /Original_Bases="AAG"
                     /label="AAG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    11266..11267
                     /Original_Bases="CG"
                     /label="CG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    11270^11271
                     /Original_Bases="GT"
                     /label="GT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    11303^11304
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
ORIGIN      
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta ggggtcatca gatttcggtg acgggcagga ccggacgggg
      661 cggcaccggc aggctgaagt ccagctgcca gaaacccacg tcatgccagt tcccgtgctt
      721 gaagccggcc gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac
      781 gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc ttgaagccct gtgcctccag
      841 ggacttcagc aggtgggtgt agagcgtgga gcccagtccc gtccgctggt ggcgggggga
      901 gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc agggacccgc
      961 gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg agccagggat agcgctcccg
     1021 cagacggacg aggtcgtccg tccactcctg cggttcctgc ggctcggtac ggaagttgac
     1081 cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt
     1141 ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatg gtagatcccc tcgatcgagt
     1201 tgagagtgaa tatgagactc taattggata ccgaggggaa tttatggaac gtcagtggag
     1261 catttttgac aagaaatatt tgctagctga tagtgacctt aggcgacttt tgaacgcgca
     1321 ataatggttt ctgacgtatg tgcttagctc attaaactcc agaaacccgc ggctcagtgg
     1381 ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg
     1441 gcgggggtca taacgtgact cccttaattc tccgctcatg atcgataatt cgcggtaccc
     1501 ggggatcctc tagagggccc gacgtcgcat gctcccggcc gccatggccg cgggatatca
     1561 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     1621 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     1681 tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
     1741 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
     1801 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     1861 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     1921 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     1981 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
     2041 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     2101 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     2161 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     2221 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     2281 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     2341 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     2401 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg
     2461 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     2521 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     2581 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     2641 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     2701 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     2761 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
     2821 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     2881 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     2941 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     3001 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     3061 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     3121 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
     3181 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     3241 ttcagctttc ttgtacaaag tggtgatatc actagtgcgg ccgcctgcag gtcgaccata
     3301 tgggagagct caagcttgca tgcctgcagg tcactggatt ttggttttag gaattagaaa
     3361 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
     3421 acacatgagc gaaaccctat aagaacccta attcccttat ctgggaacta ctcacacatt
     3481 attctggaga aaaatagaga gagatagatt tgtagagaga gactggtgat ttttgcggac
     3541 tctagaacta gtggatcccc cgggctgcag ccgggcggcg cttacagctc gtccttcttg
     3601 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
     3661 tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg
     3721 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
     3781 tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc
     3841 tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc
     3901 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
     3961 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
     4021 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
     4081 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc
     4141 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
     4201 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
     4261 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catgtcggcc
     4321 gaggataatg ataggagaag tgaaaagatg agaaagagaa aaagattagt cttcaccatg
     4381 gctatcgttc gtaaatggtg aaaattttca gaaaattgct tttgctttaa aagaaatgat
     4441 ttaaattgct gcaatagaag tagaatgctt gattgcttga gattcgtttg ttttgtatat
     4501 gttgtgttga gaattcgagc tcggtacccg gggatcctct attgtaggtg cgaattttct
     4561 ctgctcaaat tgttgaggtt agcggatttg taaacgcgtt tatatgggct gcttggaggg
     4621 tacttttgga ttaatttttt tctgccagcg cattctgacg cggcaccgct ttggaaagtg
     4681 cgctgtgggt ccgcgttttc tacaataatg tgccgatccg gtcagaaagt atatggatga
     4741 gttgtgccag cctcaccaac gtgctgcagg cccatcatga ctacttcaat gttaatgggg
     4801 gtaatgaata aataggcgaa attgggttca cggtgggccc agggaatata atattgccgc
     4861 agaggtagtc ggatgccaag gcccgcaact aatagttcac gaacaaattc attgtagtgg
     4921 gggcatgcaa gctgacctgc aggcatgcaa gcttagcttg agcttggatc agattgtcgt
     4981 ttcccgcctt cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag
     5041 agaaaagagc gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt
     5101 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttaaa
     5161 gtactttaaa gtactttaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg
     5221 cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac
     5281 gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat
     5341 aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc
     5401 tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc
     5461 cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg
     5521 cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt
     5581 gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat
     5641 ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc
     5701 ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat
     5761 catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc
     5821 ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg
     5881 ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc
     5941 acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga
     6001 ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca
     6061 agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag
     6121 gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg
     6181 ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg
     6241 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc
     6301 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg
     6361 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg
     6421 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg
     6481 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg
     6541 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg
     6601 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg
     6661 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg
     6721 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg
     6781 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt
     6841 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg
     6901 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg
     6961 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag
     7021 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac
     7081 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga
     7141 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct
     7201 atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa
     7261 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg
     7321 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc
     7381 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc
     7441 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc
     7501 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca
     7561 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc
     7621 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga
     7681 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg
     7741 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc
     7801 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc
     7861 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt
     7921 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca
     7981 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg
     8041 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag
     8101 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc
     8161 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc
     8221 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc
     8281 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc
     8341 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc
     8401 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg
     8461 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca
     8521 aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata gcacgtacat
     8581 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta
     8641 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc
     8701 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact
     8761 gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc
     8821 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg
     8881 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg
     8941 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     9001 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     9061 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     9121 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     9181 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     9241 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     9301 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9361 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     9421 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     9481 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     9541 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     9601 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     9661 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     9721 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     9781 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     9841 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     9901 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9961 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
    10021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
    10081 tttggtcatg catgatatat ctcccaattt gtgtagggct tattatgcac gcttaaaaat
    10141 aataaaagca gacttgacct gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta
    10201 acgcttgagt taagccgcgc cgcgaagcgg cgtcggcttg aacgaatttc tagctagaca
    10261 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
    10321 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
    10381 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
    10441 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
    10501 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
    10561 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
    10621 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
    10681 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
    10741 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
    10801 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
    10861 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
    10921 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
    10981 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
    11041 cgcttccccc atgatgttta actttgtttt agggcgactg ccctgctgcg taacatcgtt
    11101 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
    11161 aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
    11221 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
    11281 gcattacagc ttacgaaccg aacaggctta tgtccactgg gttcgtgccc gaat
//
