LOCUS       pBGW                    9697 bp    DNA     circular     12-JUL-2022
DEFINITION  .
ACCESSION   urn.local...1wq-dspbruq
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     http://www.informaxinc.com/                                        
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|342047972|                                                 
            VNTDBDATE|457829993|                                               
            LSOWNER|                                                           
            VNTNAME|pBGW,0|                                                    
            VNTAUTHORNAME|Demo User|                                           
            VNTAUTHOREML|manka@psb.ugent.be|                                   
            VNTOAUTHORNAME|Mansour Karimi|                                     
            VNTOAUTHORTEL|+3193313850|                                         
            VNTOAUTHOREML|mansour.karimi@psb.ugent.be|                         
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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                          (CWidget 1 (1 #1# 1) 1 2 0 0 #161# 0 100)            
                               (LOGPEN 0 10 0) 8 1 6.27319 1.9 0.123113 1)     
                                #243=(CLabel                                   
                                      (CWidget 0 (0 0) 1 2 0 0 #190# 28915308
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 47 17 0 0 400 255 1 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0 "{\\i Apa}I (1)"                                "@N
            (@S)" 5 2.17669 15.0549 0 -10 2.15833                              
             0.694805 #242#)) (CObjectList))                       
            #244=(CGroupWidget                                                 
                  (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -351 100)                  
                  (CObjectList                                                 
                   #245=(CScratch                                              
                         (CWidget 1 (1 #5# 1) 1 2 0 0 #161# 1195460419         
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                                   (CWidget 0 (0 0) 1 2 0 0 #190# 28900116 100)
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                                           (LOGFONT 47 17 0 0 400 255 1 0 0 3 2
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                                                "{\\i Cla}I (5048)" "@N (@S)" 5
            -4.66831                                -12.7388 0 -10 2.9714
            0.694805 #245#))                         (CObjectList))            
                                               #247=(CGroupWidget              
                                                     (CWidget 1 (10 #6# 0) 1 2
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                                                             (CWidget 1 (1 #6#
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                  (CWidget 0 (0 0) 1 2 0 0 #190# 18023056 100)                 
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                               "{\\i Eco}RI (488)" "@N (@S)" 5 11.2378         
                                  9.26456 0 -10 3.17836 0.694805 #248#))       
                             (CObjectList))                                    
                       #250=(CGroupWidget                                      
                             (CWidget 1 (10 #8# 0) 1 2 0 0 Nil -1291 100)      
                              (CObjectList                                     
                               #251=(CScratch                                  
                                     (CWidget 1 (1 #8# 1) 1 2 0 0 #161# 0 100) 
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                                                       (CWidget 0 (0 0) 1 2 0 0
            #190# 29079644 100)                                (LOGPEN 0 0
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            "{\\i Hin}dIII (1794)" "@N (@S)" 5 14.48                           
                -3.47421 0 -10 3.6662 0.694805 #251#))                        
            (CObjectList))                                               
            #253=(CGroupWidget                                                 
                  (CWidget 1 (10 #14# 0) 1 2 0 0 Nil -1996 100)                
                    (CObjectList                                               
                     #254=(CScratch                                            
                           (CWidget 1 (1 #14# 1) 1 2 0 0 #161# 0 100)          
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                                       (CWidget 0 (0 0) 1 2 0 0 #190# 18023016
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            0.611111 0                                        "{\\i Sac}I
            (1792)" "@N (@S)" 5 14.4282                                -2.31614
            0 -10 3.07488 0.694805 #254#))                        
            (CObjectList))                                               
            #256=(CGroupWidget                                                 
                  (CWidget 1 (10 #18# 0) 1 2 0 0 Nil -194 100)                 
                   (CObjectList                                                
                    #257=(CScratch                                             
                          (CWidget 1 (1 #18# 1) 1 2 0 0 #161# 41617208         
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                                           (CWidget 0 (0 0) 1 2 0 0 #190#
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            0.611111 0                                        "{\\i Spe}I
            (1753)" "@N (@S)" 5 14.5992 0 0                                -10
            3.11923 0.694805 #257#)) (CObjectList))                  
            #259=(CGroupWidget                                                 
                  (CWidget 1 (10 #20# 0) 1 2 0 0 Nil -588 100)                 
                   (CObjectList                                                
                    #260=(CScratch                                             
                          (CWidget 1 (1 #20# 1) 1 2 0 0 #161# 41617048         
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                                #261=(CLabel                                   
                                      (CWidget 0 (0 0) 1 2 0 0 #190# 41097892
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            0.611111 0                                        "{\\i Xba}I
            (9660)" "@N (@S)" 5 -2.54883                                13.8968
            0 -10 3.16358 0.694805 #260#))                        
            (CObjectList))                                               
            #262=(CGroupWidget                                                 
                  (CWidget 2 (10 #7# 0) 1 2 0 0 Nil -1134 100)                 
                   (CObjectList                                                
                    #263=(CScratch                                             
                          (CWidget 1 (1 #7# 1) 1 2 0 0 #161# 0 100)            
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                                #264=(CLabel                                   
                                      (CWidget 0 (0 0) 1 2 0 0 #189# 28734756
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            (38)" "@N (@S)" 5 8.11097                                11.5807 0
            -10 3.03053 0.694805 #263#)                          #265=(CScratch
                                                                       (CWidget
            2 (1 #7# 2) 1 2 0 0 #161# 41617288                                
            100) (LOGPEN 0 10 0) 8 1 5.13909 1.9                               
            0.123113 1)                                                  
            #266=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #189# 18023016 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Eco}RV (1749)" "@N (@S)" 5 14.8869        
                                   1.15807 0 -10 3.68099 0.694805 #265#))      
                              (CObjectList))                                   
                        #267=(CGroupWidget                                     
                              (CWidget 2 (10 #9# 0) 1 2 0 0 Nil -1369 100)     
                               (CObjectList                                    
                                #268=(CScratch                                 
                                      (CWidget 1 (1 #9# 1) 1 2 0 0 #161# 0 100)
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            1 2 0 0 #189# 0 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 47
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            "Arial") 1.15807 0.611111 0                                       
            "{\\i Nco}I (24)" "@N (@S)" 5 4.4367 12.7388                       
                    0 -10 2.48356 0.694805 #268#)                              
              #270=(CScratch                                                   
                    (CWidget 2 (1 #9# 2) 1 2 0 0 #161# 0 100)                  
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                                (CWidget 0 (0 0) 1 2 0 0 #189# 41014444 100)   
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                                              "{\\i Nco}I (789)" "@N (@S)" 5
            11.9934                                8.10649 0 -10 2.80879
            0.694805 #270#))                         (CObjectList))            
                                               #272=(CGroupWidget              
                                                     (CWidget 2 (10 #13# 0) 1 2
            0 0 Nil -507 100)                         (CObjectList             
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                  (CWidget 0 (0 0) 1 2 0 0 #189# 40755492 100)                 
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                               "{\\i Pst}I (1614)" "@N (@S)" 5 14.1326         
                                  3.47421 0 -10 2.8827 0.694805 #273#)         
                            #275=(CScratch                                     
                                  (CWidget 2 (1 #13# 2) 1 2 0 0 #161# 0 100)   
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                                                       (CWidget 0 (0 0) 1 2 0 0
            #189# 40958412 100)                                (LOGPEN 0 0
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            "{\\i Pst}I (1771)" "@N (@S)" 5 14.4816                            
               -1.15807 0 -10 2.8827 0.694805 #275#))                        
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            #277=(CGroupWidget                                                 
                  (CWidget 2 (10 #17# 0) 1 2 0 0 Nil -116 100)                 
                   (CObjectList                                                
                    #278=(CScratch                                             
                          (CWidget 1 (1 #17# 1) 1 2 0 0 #161# 0 100)           
                                (LOGPEN 0 10 0) 8 1 5.39212 1.9 0.123113 1)    
                                 #279=(CLabel (CWidget 0 (0 0) 1 2 0 0 #189# 16
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 47 17 0 0 400 255 0 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Sma}I
            (1359)" "@N (@S)" 5 13.9735                                4.63228
            0 -10 3.29663 0.694805 #278#)                         
            #280=(CScratch                                                     
                  (CWidget 2 (1 #17# 2) 1 2 0 0 #161# 41671240                 
                           100) (LOGPEN 0 10 0) 8 1 0.0123271 1.9              
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                             (CWidget 0 (0 0) 1 2 0 0 #189# 268634488 100)     
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                                            "Arial") 1.15807 0.611111 0        
                                           "{\\i Sma}I (9651)" "@N (@S)" 5
            -4.83044                                12.7388 0 -10 3.29663
            0.694805 #280#))                         (CObjectList))            
                                               #282=(CGroupWidget              
                                                     (CWidget 2 (10 #22# 0) 1 2
            0 0 Nil -1449 100)                         (CObjectList            
                                                        #283=(CScratch         
                                                              (CWidget 1 (1
            #22# 1) 1 2 0 0 #161#                                  1094796097
            100) (LOGPEN 0 10 0) 8 1 5.39342                                1.9
            0.123113 1)                                              
            #284=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #189# 41729380 100)                 
                          (LOGPEN 0 0 8437824) 1                               
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                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Xma}I (1357)" "@N (@S)" 5 13.5372         
                                  5.79035 0 -10 3.29663 0.694805 #283#)        
                             #285=(CScratch                                    
                                   (CWidget 2 (1 #22# 2) 1 2 0 0 #161# 0 100)  
                                         (LOGPEN 0 10 0) 8 1 0.0136247 1.9
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            1 2 0 0 #189# 1 100)                                (LOGPEN 0 0
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            "{\\i Xma}I (9649)" "@N (@S)" 5 -8.19782                           
                11.5807 0 -10 3.29663 0.694805 #285#))                        
            (CObjectList))) (CObjectList))                        
            #287=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -979 100)        
                     (CObjectList) (CObjectList))                              
               #288=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -431 100)      
                       (CObjectList) (CObjectList))) (CObjectList)))        
            (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList)) (CObList)
            84410469    (CStringList) 1096810496 327685 (CObList))).
FEATURES             Location/Qualifiers
     T-DNA_border    1..333
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     70..89
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="5985363896116000ea926926-3939-40be-a57a
                     -be0fb3ec6679"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P8"
     terminator      complement(354..621)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     Plant_Selection complement(354..1472)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1k-e500sax"">Basta</a>"
                     /label="Basta"
     misc_feature    370^371
                     /Original_Bases="TAG"
                     /label="TAG"
                     /note="Geneious type: Editing History Deletion"
     CDS             complement(628..1179)
                     /%_Identity=100
                     /Motif="ATGAGCCCAGAACGACGCCCGGCCGACATCCGCCGTGCCACCGAGGCGGA
                     CATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAGACAAGCACGGTCAACTTC
                     CGTACCGAGCCGCAGGAACCGCAGGAGTGGACGGACGACCTCGTCCGTCTGCGGGAGC
                     GCTATCCCTGGCTCGTCGCCGAGGTGGACGGCGAGGTCGCCGGCATCGCCTACGCGGG
                     TCCCTGGAAGGCACGCAACGCCTACGACTGGACGGCCGAGTCGACCGTGTACGTCTCC
                     CCCCGCCACCAGCGGACGGGACTGGGCTCCACGCTCTACACCCACCTGCTGAAGTCCC
                     TGGAGGCACAGGGCTTCAAGAGCGTGGTCGCTGTCATCGGGCTGCCCAACGACCCGAG
                     CGTGCGCATGCACGAGGCGCTCGGATATGCCCCCCGCGGCATGCTGCGGGCGGCCGGC
                     TTCAAGCACGGGAACTGGCATGACGTGGGTTTCTGGCAGCTGGACTTCAGCCTGCCGG
                     TGCCGCCCCGTCCGGTCCTGCCCGTCACCGAAATCTGA"
                     /annotation_group="PAT: 8,732 -> 9,283"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PAT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:98-dgycryy"">PAT</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; PAT
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /label="PAT"
     primer_bind     845..864
                     /%GC="55.0"
                     /Hairpin_Tm="45.2"
                     /Self_Dimer_Tm="None"
                     /Tm="59.0"
                     /annotation_group="59853639527738007c251b32-544c-4b53-bb26
                     -4ab5f7bb68b6"
                     /created_by="primer3"
                     /Sequence="TTCAGCAGGTGGGTGTAGAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P9"
     promoter        complement(1200..1472)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     misc_feature    1473..1477
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1481..1483
                     /Original_Bases="TAT"
                     /label="TAT"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1493
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1495^1496
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     attR1           1560..1684
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c-f0o2y6y"">attR1</a>"
     promoter        1709..1739
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    1763
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1793..2452
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     1948..1967
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.8"
                     /annotation_group="598536401002780074893a5a-0196-4b5a-abcb
                     -5fa6f7883d63"
                     /created_by="primer3"
                     /Sequence="GCACAAGTTTTATCCGGCCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P12"
     primer_bind     complement(2023..2042)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5985364348234700f4ad6d53-4ca1-44a6-b22c
                     -02c69f39267f"
                     /created_by="primer3"
                     /Sequence="TCACCAGCTCACCGTCTTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P16"
     misc_feature    2377
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     UTR             2475..2793
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    2567
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     2731..2750
                     /%GC="55.0"
                     /Hairpin_Tm="35.1"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="5985364066988600d89d8263-0160-4cc9-b319
                     -c011635a2697"
                     /created_by="primer3"
                     /Sequence="CTGAGGTCGCCCGGTTTATT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P13"
     misc_feature    2785
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     CDS             2794..3099
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     attR2           complement(3140..3264)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     T-DNA_border    3313..3513
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="99.50%"
                     /Primary_Match="99.50%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:3c-djznfva"">RB</a>"
                     /label="RB"
     primer_bind     3334..3353
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5985364232752700a5cbb224-a275-431d-9527
                     -865414fabf40"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P14"
     misc_feature    3428
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    3521..3550
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     4162..4180
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="598536347632250007f43825-43f1-4016-bc61
                     -a5374f9c8c41"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P1"
     regulatory      4729..5358
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     4954..4973
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="598536353767840032e101ae-e8d0-40b4-a7bf
                     -b2068634d22d"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P2"
     primer_bind     5751..5769
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="598536359392170014427cd0-6cf1-4d53-aeb8
                     -266eccdfe27d"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P3"
     regulatory      5787..6860
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     misc_feature    5791
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     6555..6572
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="5985363659896500e890b34e-5055-474a-bf84
                     -fe7566bd42bd"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P4"
     rep_origin      6926..7120
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     primer_bind     7355..7374
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="59853637233719001cb17e2f-c82b-472b-94e1
                     -b8b3de402ebb"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P5"
     regulatory      7494..7754
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /label="pBR322 bom"
     rep_origin      complement(7790..8378)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     8179..8198
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="5985363780582900ea73640e-ef1f-4227-b1fe
                     -14975588bedd"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P6"
     misc_feature    8564^8565
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    8576^8577
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    8581..8584
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    complement(8624..9415)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     8951..8970
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="5985363839328900447781c6-eb67-4055-bc33
                     -132a7f350fa0"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pBGW"
                     /label="P7"
     Promoter        complement(9416..9690)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    9550^9551
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    9561^9562
                     /Original_Bases="AAG"
                     /label="AAG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    9629..9630
                     /Original_Bases="CG"
                     /label="CG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    9633^9634
                     /Original_Bases="GT"
                     /label="GT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    9666^9667
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
ORIGIN      
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta ggggtcatca gatttcggtg acgggcagga ccggacgggg
      661 cggcaccggc aggctgaagt ccagctgcca gaaacccacg tcatgccagt tcccgtgctt
      721 gaagccggcc gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac
      781 gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc ttgaagccct gtgcctccag
      841 ggacttcagc aggtgggtgt agagcgtgga gcccagtccc gtccgctggt ggcgggggga
      901 gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc agggacccgc
      961 gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg agccagggat agcgctcccg
     1021 cagacggacg aggtcgtccg tccactcctg cggttcctgc ggctcggtac ggaagttgac
     1081 cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt
     1141 ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatg gtagatcccc tcgatcgagt
     1201 tgagagtgaa tatgagactc taattggata ccgaggggaa tttatggaac gtcagtggag
     1261 catttttgac aagaaatatt tgctagctga tagtgacctt aggcgacttt tgaacgcgca
     1321 ataatggttt ctgacgtatg tgcttagctc attaaactcc agaaacccgc ggctcagtgg
     1381 ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg
     1441 gcgggggtca taacgtgact cccttaattc tccgctcatg atcgataatt cgcggtaccc
     1501 ggggatcctc tagagggccc gacgtcgcat gctcccggcc gccatggccg cgggatatca
     1561 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     1621 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     1681 tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
     1741 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
     1801 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     1861 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     1921 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     1981 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
     2041 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
     2101 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
     2161 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
     2221 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
     2281 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
     2341 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
     2401 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaacgcgtgg
     2461 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     2521 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     2581 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     2641 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     2701 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     2761 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
     2821 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     2881 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
     2941 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     3001 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     3061 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     3121 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
     3181 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     3241 ttcagctttc ttgtacaaag tggtgatatc actagtgcgg ccgcctgcag gtcgaccata
     3301 tgggagagct caagcttagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta
     3361 aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt
     3421 agaataatcg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
     3481 catgccaacc acagggttcc cctcgggatc aaagtacttt aaagtacttt aaagtacttt
     3541 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     3601 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     3661 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     3721 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     3781 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     3841 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     3901 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     3961 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     4021 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     4081 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     4141 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     4201 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     4261 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     4321 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     4381 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     4441 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     4501 cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc
     4561 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     4621 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     4681 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     4741 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     4801 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     4861 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     4921 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     4981 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     5041 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     5101 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     5161 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     5221 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     5281 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     5341 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     5401 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     5461 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     5521 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     5581 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     5641 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     5701 ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact
     5761 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     5821 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     5881 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     5941 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     6001 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     6061 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     6121 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
     6181 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
     6241 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
     6301 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
     6361 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
     6421 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
     6481 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
     6541 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
     6601 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
     6661 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
     6721 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
     6781 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
     6841 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
     6901 aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     6961 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     7021 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     7081 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     7141 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     7201 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     7261 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     7321 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     7381 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     7441 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     7501 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     7561 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     7621 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7681 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7741 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7801 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7861 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7921 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7981 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8041 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8101 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8161 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8221 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8281 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8341 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8401 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcatgata
     8461 tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa gcagacttga
     8521 cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcttg agttaagccg
     8581 cgccgcgaag cggcgtcggc ttgaacgaat ttctagctag acattatttg ccgactacct
     8641 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
     8701 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
     8761 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     8821 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
     8881 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
     8941 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
     9001 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
     9061 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
     9121 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
     9181 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
     9241 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
     9301 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
     9361 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc cccatgatgt
     9421 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
     9481 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
     9541 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
     9601 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa
     9661 ccgaacaggc ttatgtccac tgggttcgtg cccgaat
//
