LOCUS       pJCGLOX                17560 bp    DNA     circular     23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...7a-duy8qeo
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is best viewed with a free Vector NTI Viewer:            
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|342175875|                                                 
            VNTDBDATE|458479371|                                               
            LSOWNER|                                                           
            VNTNAME|pJCGLOX,0|                                                 
            VNTAUTHORNAME|Demo User|                                           
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FEATURES             Location/Qualifiers
     Promoter        8..282
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    31^32
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    64^65
                     /Original_Bases="AC"
                     /label="AC"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    68..69
                     /Original_Bases="CG"
                     /label="CG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    139^140
                     /Original_Bases="CTT"
                     /label="CTT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    147^148
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     primer_bind     234..253
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="CAACGATGTTACGCAGCAGG"
                     /Tm="59.9"
                     /%GC="55.0"
                     /Hairpin_Tm="36.7"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P159"
     misc_feature    283..942
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="99.55%"
                     /Primary_Match="99.55%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     misc_feature    434
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1009..1012
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    1016^1017
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    1028^1029
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Deletion"
     rep_origin      1215..1803
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     1285..1304
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="5.1"
                     /Tm="60.0"
                     /annotation_group="110464164753510053063e4c-f181-4097-920c
                     -25ddb2cf4ff8"
                     /created_by="primer3"
                     /Sequence="TGTTTGCCGGATCAAGAGCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /label="P43"
     primer_bind     complement(1724..1743)
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="GCGAAACCCGACAGGACTAT"
                     /Tm="59.8"
                     /%GC="55.0"
                     /Hairpin_Tm="41.8"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P160"
     regulatory      complement(1839..2099)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:30/150; <a
                     href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     primer_bind     1905..1924
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="1104641743405600ad61a7e0-d023-4554-a3e6
                     -032ce1d87dcf"
                     /created_by="primer3"
                     /Sequence="TACCGCCTTTGAGTGAGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /label="P39"
     rep_origin      2473..2667
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2733..3806)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     primer_bind     2756..2775
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="CCTCGATCAGGTTGCGGTAG"
                     /Tm="60.2"
                     /%GC="60.0"
                     /Hairpin_Tm="37.5"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P161"
     primer_bind     3570..3589
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="AAAATCTGGTTGCTCGTCGC"
                     /Tm="59.5"
                     /%GC="50.0"
                     /Hairpin_Tm="39.8"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P162"
     misc_feature    3802
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    3855
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     regulatory      complement(4235..4864)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     4367..4386
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="GGACTCAAGAATGGGCAGCT"
                     /Tm="60.0"
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P163"
     primer_bind     5154..5173
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="GGTGCGGTTTCATGCTTGTT"
                     /Tm="60.0"
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P164"
     primer_bind     5978..5997
                     /%GC="50.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="110464185395099913806424-db37-4dd5-8e04
                     -a19bccb66b5b"
                     /created_by="primer3"
                     /Sequence="TTTCAGAAGACGGCTGCACT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /label="P37"
     T-DNA_border    6020..6248
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="90.91%"
                     /Primary_Match="90.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     misc_feature    6135
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     6357..6376
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="TTGGGTAACGCCAGGGTTTT"
                     /Tm="60.1"
                     /%GC="50.0"
                     /Hairpin_Tm="39.7"
                     /Self_Dimer_Tm="5.4"
                     /modified_by="mamee"
                     /label="P147"
     primer          6392..6407
                     /invitrogen=1980000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 (-20) forward primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     (-20) forward primer (pDONRP4-P3 - M13 (-20) forward
                     primer)"
                     /label="M13 (-20) forward primer"
     promoter        6414..7264
                     /modified_by="mamee"
                     /label="pHSP18.2"
     primer_bind     7150..7172
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="ACTCGAAGAACATTCTCTCTTCA"
                     /Tm="57.3"
                     /%GC="39.1"
                     /Hairpin_Tm="45.2"
                     /Self_Dimer_Tm="11.3"
                     /modified_by="mamee"
                     /label="P148"
     CDS             join(7296..7727,7918..9354)
                     /modified_by="mamee"
                     /label="CRE-INTRON-GR"
     misc_feature    7332
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     intron          7728..7917
                     /note="castor bean catalase intron, modified"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Hyg(Intron)"
                     /Transferred_Similarity="98.42%"
                     /Primary_Match="98.42%; Adjusted-Interval-0:0/0; cat1
                     intron (<a
                     href=""urn:local:.:5f-dgycryv"">Hyg(Intron)</a>)"
                     /label="cat1 intron"
     primer_bind     7940..7959
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="GCGATCGCTGCCAGGATATA"
                     /Tm="59.8"
                     /%GC="55.0"
                     /Hairpin_Tm="38.3"
                     /Self_Dimer_Tm="31.9"
                     /modified_by="mamee"
                     /label="P149"
     misc_feature    8589
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     8737..8756
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="GCAGCAGTGAAATGGGCAAA"
                     /Tm="60.0"
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P150"
     misc_feature    8765
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8772
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8787
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8842
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    8871
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     terminator      9409..10116
                     /created_by="mamee"
                     /label="tOCS"
     misc_feature    9796
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     misc_feature    9845
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    10121
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     promoter        10215..11078
                     /created_by="mamee"
                     /label="p35S"
     primer_bind     10273..10293
                     /%GC="57.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="4.4"
                     /Tm="60.0"
                     /annotation_group="1104638730127099c295aaed-5b71-4012-9f5d
                     -07c02938c86d"
                     /created_by="primer3"
                     /Sequence="GATGGTTAGAGAGGCCTACGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /label="P10"
     primer_bind     complement(10525..10546)
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="TGACTCCATGGCCTTTGATTCA"
                     /Tm="60.0"
                     /%GC="45.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="7.0"
                     /modified_by="mamee"
                     /label="P151"
     misc_feature    11134
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Replacement"
     motif           11148..11181
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="loxP"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:5s-dgycryw"">loxP</a>"
                     /label="LoxP"
     CDS             11182..11901
                     /label="Egfp"
     terminator      11908..12615
                     /created_by="mamee"
                     /label="tOCS"
     motif           12616..12649
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="loxP"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:5s-dgycryw"">loxP</a>"
                     /label="LoxP"
     attR1           12661..12785
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c-f0o2y6y"">attR1</a>"
     promoter        12810..12840
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    12864
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    12894..13553
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     misc_feature    13478
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     13556..13575
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="GCGTGGATCCGGCTTACTAA"
                     /Tm="59.9"
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="17.8"
                     /modified_by="mamee"
                     /label="P154"
     UTR             13576..13894
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    13668
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(13690..13709)
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="TGTCGCTGTCAACTGTCACT"
                     /Tm="59.5"
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P155"
     misc_feature    13886
                     /Original_Bases="A"
                     /label="A"
                     /note="Geneious type: Editing History Replacement"
     CDS             13895..14200
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     attR2           complement(14241..14365)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     terminator      14395..15102
                     /created_by="mamee"
                     /label="tOCS"
     misc_feature    15107^15108
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     primer          complement(15148..15164)
                     /invitrogen=2010000
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pDONRP4-P3 - M13 reverse primer"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     reverse primer (pDONRP4-P3 - M13 reverse primer)"
                     /label="M13 reverse primer"
     misc_feature    15147^15148
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Deletion"
     primer_bind     15178..15197
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="11046380060348003f88630c-880b-443d-9723
                     -7ec5d34d10aa"
                     /created_by="primer3"
                     /Sequence="TCCGCTCACAATTCCACACA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /label="P19"
     promoter        complement(15196..15226)
                     /note="promoter for the E. coli lac operon"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pLac"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:5d-dgycryv"">pLac</a>"
                     /label="pLAc"
     primer_bind     complement(15319..15338)
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="CGCGTTGGCCGATTCATTAA"
                     /Tm="59.6"
                     /%GC="50.0"
                     /Hairpin_Tm="39.6"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P156"
     Plant_Selection 15355..17227
                     /created_by="mamee"
                     /label="Kana"
     promoter        15355..16144
                     /modified_by="mamee"
                     /label="p35S"
     misc_feature    15704^15705
                     /Original_Bases="TA"
                     /label="TA"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    16083
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     CDS             16192..16989
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NptII"
                     /Transferred_Similarity="99.25%"
                     /Adjusted_Transferred_Similarity="98.87%"
                     /Primary_Match="99.25%; Adjusted-Interval-0:3/0 (adjusted
                     to 98.87%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:.:94-dgycryy"">NptII</a>"
                     /label="NptII"
     misc_feature    16305
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     16324..16343
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="CGCCCGGTTCTTTTTGTCAA"
                     /Tm="59.6"
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P157"
     primer_bind     complement(16504..16523)
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /Sequence="GCAGGAGCAAGGTGAGATGA"
                     /Tm="59.7"
                     /%GC="55.0"
                     /Hairpin_Tm="41.6"
                     /Self_Dimer_Tm="None"
                     /modified_by="mamee"
                     /label="P158"
     terminator      17040..17227
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="35S polyA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:60-dgycryw"">35S polyA</a>"
                     /label="35S polyA"
     primer_bind     17060..17083
                     /%GC="45.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.5"
                     /annotation_group="1104642102624099f238bb4a-2d21-48dc-89ba
                     -218f4b68a249"
                     /created_by="primer3"
                     /Sequence="TGTGTGAGTAGTTCCCAGATAAGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pJCGLOX"
                     /label="P65R"
     T-DNA_border    17228..17560
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="99.70%"
                     /Primary_Match="99.70%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
ORIGIN      
        1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
       61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
      121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
      181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
      241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggagaa aaaaatcact
      301 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      361 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      421 accgtaaaga aaagtaagca caagttttat ccggccttta ttcacattct tgcccgcctg
      481 atgaatgctc atccggagtt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      541 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      601 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      661 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
      721 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
      781 ttcgcccccg ttttcacaat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
      841 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
      901 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
      961 tggtgcgatc ctctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     1021 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
     1081 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc
     1141 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1321 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     1381 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1441 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1501 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     1561 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     1621 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     1681 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1741 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     1801 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     1861 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     1921 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     1981 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     2041 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     2101 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     2161 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     2221 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
     2281 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
     2341 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
     2401 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
     2461 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
     2521 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
     2581 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
     2641 gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct
     2701 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
     2761 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
     2821 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
     2881 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
     2941 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
     3001 aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac
     3061 atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc
     3121 ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc
     3181 ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg
     3241 tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt
     3301 ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg
     3361 gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc
     3421 ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct
     3481 cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg
     3541 gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc
     3601 ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca
     3661 gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg
     3721 gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc
     3781 gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat
     3841 tgcagggccg gcaggcaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
     3901 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
     3961 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
     4021 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
     4081 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
     4141 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
     4201 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
     4261 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
     4321 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
     4381 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
     4441 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
     4501 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
     4561 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
     4621 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
     4681 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
     4741 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
     4801 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
     4861 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
     4921 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
     4981 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
     5041 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
     5101 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
     5161 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
     5221 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
     5281 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
     5341 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
     5401 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
     5461 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
     5521 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
     5581 gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct
     5641 agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc
     5701 gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg
     5761 tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc
     5821 agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta
     5881 ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca
     5941 gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa
     6001 cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga
     6061 ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc
     6121 cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac ccgccaatat
     6181 atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg atccaagctc
     6241 aagctgctct agcattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     6301 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     6361 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagcttttc
     6421 tcttcatttc tctttctttc tttctttcat gcaagttcta ttgattagaa accaaaacca
     6481 tgacatatca cattttcaat ttttaacccc aagtttccca aaaaggtaat cacaattact
     6541 ttccatggtc atttcttctg gttcaagcat gacatgaaca ggcaataaat aagttgagat
     6601 tttgatcaca gtaactgata cttgaatcga atcatttaga tttttttttt ttttagttta
     6661 cttgtttagt aaatatgttg tctatgtttg tcacaaaaac gtggctcagt tcttgtatat
     6721 atggagacaa aaaaatccat taaaagattg ttgacattct cggaaattta gtgccaactg
     6781 ttattgcgag aacttactat agttttcctt tggcgaaaag ctaataatct taaatcttga
     6841 ttttgtcctc ttttctctga gttagatttt cttaaattcc acttccgacc tattaagaaa
     6901 tgggcttttg caaagaagat ccgcttcact gagcccgtat ctcgaagagg ataatacaac
     6961 aacaaagcaa aacggcacgt agttttaatt gtaaccaagg attgcatttc ggtcttgttt
     7021 caacaaacga aacttcctga aatgccaaga aaaatctggt catttcacca cagtgatcat
     7081 tgtgtatgtg ttctaaagac tccaagcgaa ggttttagaa aaaggagcat tttctattct
     7141 attcaagaaa ctcgaagaac attctctctt catcctctaa cttccctata aatatgtcct
     7201 ttgctaatca gatcaaatca gcaggaaaat caagaaccaa aagtctcccg aaaagcaacg
     7261 aacagatatc acaagtttgt acaaaaaagc aggctatgtc caatttactg accgtacacc
     7321 aaaatttgcc tacattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg
     7381 acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt
     7441 gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg
     7501 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc
     7561 agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg
     7621 acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg
     7681 aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggta aatttctagt
     7741 ttttctcctt cattttcttg gttaggaccc ttttctcttt ttattttttt gagctttgat
     7801 ctttctttaa actgatctat tttttaattg attggttatg gtgtaaatat tacatagctt
     7861 taactgataa tctgattact ttatttcgtg tgtctatgat gatgatgata gttacaggtt
     7921 cgttcactca tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg
     7981 attgcttata acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc
     8041 tcacgtactg acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc
     8101 accacaggtg tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt
     8161 tccgtctctg gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat
     8221 ggtgttgccg cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt
     8281 gaagcaactc atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc
     8341 tggtctggac acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca
     8401 ataccggaga tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc
     8461 cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgatgagctc
     8521 attcagcaag ccactgcagg agtctcacaa gacacttcgg aaaatcctaa caaaacaata
     8581 gttcctgctg cattaccaca gctcacccct accttggtgt cactgctgga ggtgattgaa
     8641 cccgaggtgt tgtatgcagg atatgatagc tctgttccag attcagcatg gagaattatg
     8701 accacactca acatgttagg tgggcgtcaa gtgattgcag cagtgaaatg ggcaaaggcg
     8761 ataccaggct tcagaaactt acacctggat gaccaaatga ccctgctaca gtactcatgg
     8821 atgtttctca tggcatttgc cctgggttgg agatcataca gacaatcaag tggaaacctg
     8881 ctctgctttg ctcctgatct gattattaat gagcagagaa tgtctctacc ctgcatgtat
     8941 gaccaatgta aacacatgct gtttgtctcc tctgaattac aaagattgca ggtatcctat
     9001 gaagagtatc tctgtatgaa aaccttactg cttctctcct cagttcctaa ggaaggtctg
     9061 aagagccaag agttatttga tgagattcga atgacttata tcaaagagct aggaaaagcc
     9121 atcgtcaaaa gggaagggaa ctccagtcag aactggcaac ggttttacca actgacaaag
     9181 cttctggact ccatgcatga ggtggttgag aatctcctta cctactgctt ccagacattt
     9241 ttggataaga ccatgagtat tgaattccca gagatgttag ctgaaatcat cactaatcag
     9301 ataccaaaat attcaaatgg aaatatcaaa aagcttctgt ttcatcaaaa atgaacccag
     9361 ctttcttgta caaagtggtg atatccatca cactggcggc cgctcgagct gctttaatga
     9421 gatatgcgag acgcctatga tcgcatgata tttgctttca attctgttgt gcacgttgta
     9481 aaaaacctga gcatgtgtag ctcagatcct taccgccggt ttcggttcat tctaatgaat
     9541 atatcacccg ttactatcgt atttttatga ataatattct ccgttcaatt tactgattgt
     9601 accctactac ttatatgtac aatattaaaa tgaaaacaat atattgtgct gaataggttt
     9661 atagcgacat ctatgataga gcgccacaat aacaaacaat tgcgttttat tattacaaat
     9721 ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa gactgattac ataaatctta
     9781 ttcaaatttc aaaagtgccc caggggctag tatctacgac acaccgagcg gcgaactaat
     9841 aacgctcact gaagggaact ccggttcccc gccggcgcgc atgggtgaga ttccttgaag
     9901 ttgagtattg gccgtccgct ctaccgaaag ttacgggcac cattcaaccc ggtccagcac
     9961 ggcggccggg taaccgactt gctgccccga gaattatgca gcattttttt ggtgtatgtg
    10021 ggccccaaat gaagtgcagg tcaaaccttg acagtgacga caaatcgttg ggcgggtcca
    10081 gggcgaattt tgcgacaaca tgtcgaggct cagcagtcta gagctttcct ttgccccgga
    10141 gatcaccatg gacgactttc tctatctcta cgatctagga agaaagttcg acggagaagg
    10201 tgacgatacc atgttcacca ccgataatga gaagattagc ctcttcaatt tcagaaagaa
    10261 tgctgaccca cagatggtta gagaggccta cgcggcaggt ctcatcaaga cgatctaccc
    10321 gagtaataat ctccaggaga tcaaatacct tcccaagaag gttaaagatg cagtcaaaag
    10381 attcaggact aactgcatca agaacacaga gaaagatata tttctcaaga tcagaagtac
    10441 tattccagta tggacgattc aaggcttgct tcataaacca aggcaagtaa tagagattgg
    10501 agtctctaag aaagtagttc ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga
    10561 ggatctaaca gaactcgccg tgaagactgg cgaacagttc atacagagtc ttttacgact
    10621 caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacactctcg tctactccaa
    10681 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
    10741 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
    10801 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
    10861 tcaagatgcc cctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
    10921 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
    10981 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
    11041 aagttcattt catttggaga ggactccggt atttttacaa caattaccac aacaaaacaa
    11101 acaacaaaca acattacaat ttactattct agtcggagct cggatccata acttcgtata
    11161 gcatacatta tacgaagtta tatggtgagc aagggcgagg agctgttcac cggggtggtg
    11221 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
    11281 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
    11341 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
    11401 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
    11461 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
    11521 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
    11581 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
    11641 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
    11701 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
    11761 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
    11821 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
    11881 atggacgagc tgtacaagta aactagtctg ctttaatgag atatgcgaga cgcctatgat
    11941 cgcatgatat ttgctttcaa ttctgttgtg cacgttgtaa aaaacctgag catgtgtagc
    12001 tcagatcctt accgccggtt tcggttcatt ctaatgaata tatcacccgt tactatcgta
    12061 tttttatgaa taatattctc cgttcaattt actgattgta ccctactact tatatgtaca
    12121 atattaaaat gaaaacaata tattgtgctg aataggttta tagcgacatc tatgatagag
    12181 cgccacaata acaaacaatt gcgttttatt attacaaatc caattttaaa aaaagcggca
    12241 gaaccggtca aacctaaaag actgattaca taaatcttat tcaaatttca aaagtgcccc
    12301 aggggctagt atctacgaca caccgagcgg cgaactaata acgctcactg aagggaactc
    12361 cggttccccg ccggcgcgca tgggtgagat tccttgaagt tgagtattgg ccgtccgctc
    12421 taccgaaagt tacgggcacc attcaacccg gtccagcacg gcggccgggt aaccgacttg
    12481 ctgccccgag aattatgcag catttttttg gtgtatgtgg gccccaaatg aagtgcaggt
    12541 caaaccttga cagtgacgac aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat
    12601 gtcgaggctc agcagataac ttcgtatagc atacattata cgaagttatc tgcagatatc
    12661 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
    12721 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
    12781 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
    12841 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
    12901 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
    12961 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
    13021 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
    13081 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
    13141 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
    13201 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
    13261 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
    13321 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
    13381 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
    13441 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
    13501 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
    13561 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
    13621 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
    13681 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
    13741 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
    13801 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
    13861 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
    13921 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
    13981 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
    14041 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
    14101 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
    14161 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
    14221 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
    14281 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
    14341 gttcagcttt cttgtacaaa gtggtgatat ccatcacact ggcggccgct cgagctgctt
    14401 taatgagata tgcgagacgc ctatgatcgc atgatatttg ctttcaattc tgttgtgcac
    14461 gttgtaaaaa acctgagcat gtgtagctca gatccttacc gccggtttcg gttcattcta
    14521 atgaatatat cacccgttac tatcgtattt ttatgaataa tattctccgt tcaatttact
    14581 gattgtaccc tactacttat atgtacaata ttaaaatgaa aacaatatat tgtgctgaat
    14641 aggtttatag cgacatctat gatagagcgc cacaataaca aacaattgcg ttttattatt
    14701 acaaatccaa ttttaaaaaa agcggcagaa ccggtcaaac ctaaaagact gattacataa
    14761 atcttattca aatttcaaaa gtgccccagg ggctagtatc tacgacacac cgagcggcga
    14821 actaataacg ctcactgaag ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc
    14881 ttgaagttga gtattggccg tccgctctac cgaaagttac gggcaccatt caacccggtc
    14941 cagcacggcg gccgggtaac cgacttgctg ccccgagaat tatgcagcat ttttttggtg
    15001 tatgtgggcc ccaaatgaag tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg
    15061 ggtccagggc gaattttgcg acaacatgtc gaggctcagc agtctagcta gaggatcccc
    15121 gggtaccgag ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
    15181 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    15241 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    15301 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    15361 tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc caagaatatc
    15421 aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg
    15481 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa
    15541 aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
    15601 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
    15661 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga
    15721 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
    15781 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
    15841 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
    15901 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
    15961 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
    16021 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
    16081 cccttcctct atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc
    16141 tctctctaca aatctatctc tctcgagctt tcgcagatct gtcgatcgac catggggatt
    16201 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
    16261 gactgggcac aacagacaat cggctgctct gatgccgccg tgtttcggct gtcagcgcag
    16321 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actccaggac
    16381 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
    16441 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
    16501 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
    16561 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
    16621 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
    16681 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
    16741 gatctcgtcg tgacacatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
    16801 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
    16861 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
    16921 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
    16981 ttcttctgag cgggactctg gggttcggat cgatcctcta gctagagtcg atcgacaagc
    17041 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
    17101 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
    17161 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
    17221 ccccgaatta attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
    17281 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
    17341 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
    17401 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
    17461 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
    17521 gttgattgta acgatgacag agcgttgctg cctgtgatca
//
