LOCUS       pH7WGC2                12291 bp    DNA     circular     15-FEB-2023
DEFINITION  .
ACCESSION   urn.local...19t-dsovnx5
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            VNTDATE|342173703|                                                 
            VNTDBDATE|458479493|                                               
            LSOWNER|                                                           
            VNTNAME|pH7WGC2,0|                                                 
            VNTAUTHORNAME|Demo User|                                           
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
            (SXF                                                               
             (CGexDoc "pH7WGC2,0" 0 12263                                      
              (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList)
            (CObList)     (CObList) -1 "")                                     
                         (CDocSetData 1 1 0 0 0 2 "MAIN" 1 1 1 1 0 0 1 1 0 1 10
            5 40 50 0 1 0     (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray)   
                              (CStringList "AatII" "ApaI" "AseI" "AvaI" "BamHI"
            "ClaI" "EcoRI" "EcoRV"      "HindIII" "NcoI" "NdeI" "NotI" "NruI"
            "PstI" "SacI" "SacII" "SalI"      "SmaI" "SpeI" "SphI" "XbaI"
            "XhoI" "XmaI" "BglII")                 (CStringList "atg" "gtg")
            (CStringList "taa" "tga" "tag") (CObList) 1     "{(0,1),2}" 0 0 ""
            0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0      
            (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0))       
            (CMolPar 0 0 0 0 0 1 12263 0 0 0 0 0 0 0 0) (CStringList)
            (CStringList)    (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25
            250 50 0 6 4 3 7)    (COAPar 25 250 50 0 6 4 3 7)                  
                                 (CObList                                      
                                  #0=(CRSite (CStringList) "AatII" "gacgtc" 6 0
            2 7 0 11911 0 0 "")     #1=(CRSite (CStringList) "ApaI" "gggccc" 6
            0 1 1 0 0 "")            #2=(CRSite (CStringList) "AseI" "attaat" 3
            0 1 10733 0 0 "")        #3=(CRSite (CStringList) "AvaI" "cycgrg" 2
            0 8 638 0 3515 0 3938 0 4568         0 5699 0 6179 0 9923 0 12243 0
            0 "")                            #4=(CRSite (CStringList) "BamHI"
            "ggatcc" 2 0 3 1054 0 1757 0 12248 0 0         "") #5=(CRSite
            (CStringList) "ClaI" "atcgat" 3 0 1 7003 0 0 "")     #6=(CRSite
            (CStringList) "EcoRI" "gaattc" 2 0 1 1507 0 0 "")        #7=(CRSite
            (CStringList) "EcoRV" "gatatc" 4 0 3 250 0 1961 0 2865 0 0 "")    
            #8=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 3749 0 0 "")     
            #9=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 1206 0 3297 0 3687 0
            0 "")     #10=(CRSite (CStringList) "NdeI" "catatg" 3 0 4 3735 0
            5468 0 7957 0          11482 0 0 "")                               
                              #11=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 5
            1827 0 2689 0 4799 0          6331 0 7621 0 0 "")                  
                                     #12=(CRSite (CStringList) "NruI" "tcgcga"
            4 0 0 0 "")               #13=(CRSite (CStringList) "PstI" "ctgcag"
            6 0 3 19 0 389 0 2700 0 0 "")     #14=(CRSite (CStringList) "SacI"
            "gagctc" 6 0 1 3747 0 0 "")        #15=(CRSite (CStringList)
            "SacII" "ccgcgg" 5 0 2 245 0 12120 0 0 "")     #16=(CRSite
            (CStringList) "SalI" "gtcgac" 2 0 3 379 0 2702 0 3728 0 0 "")    
            #17=(CRSite (CStringList) "SmaI" "cccggg" 4 0 2 640 0 12245 0 0 "")
                #18=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 2682 0 0 "")   
                #19=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 13 0 3920 0
            10456 0 0 "")     #20=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1
            12254 0 0 "")       #21=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0
            0 "")               #22=(CRSite (CStringList) "XmaI" "cccggg" 2 0 2
            638 0 12243 0 0 "")     #23=(CRSite (CStringList) "BglII" "agatct"
            2 0 0 0 ""))            (CObList                                   
                                     #24=(CFSignal (CObList) "ECFP" 4 0 1 1964
            2680 0 (CStringList)           (CStringList) 1 1 1 1 "")           
                                      #25=(CFSignal (CObList) "attR1" 86 0 1
            1833 1957 0 (CStringList)          (CStringList) 1 1 1 1 "")       
                                          #26=(CFSignal (CObList) "ccdB" 21 0 1
            378 1832 0 (CStringList)           (CStringList) 1 1 1 1 "")       
                                          #27=(CFSignal (CObList) "attR2" 86 0
            0 253 377 0 (CStringList)           (CStringList) 1 1 1 1 "")      
                                           #28=(CFSignal (CObList) "T35S" 43 0
            0 18 243 0 (CStringList)             (CStringList) 1 1 1 1 "")     
                                            #29=(CFSignal (CObList) "p35S" 29 0
            1 2702 3729 0 (CStringList)          (CStringList) 1 1 1 1 "")     
                                            #30=(CFSignal (CObList) "RB" 87 0 0
            3749 3948 0 (CStringList)            (CStringList) 1 1 1 1 "")     
                                            #31=(CFSignal (CObList) "Sm/SpR" 22
            0 1 8852 10101 0 (CStringList)          (CStringList) 1 1 1 1 "")  
                                               #32=(CFSignal (CObList) "LB" 87
            0 0 10107 10439 0 (CStringList)          (CStringList) 1 1 1 1 "") 
                                                #33=(CFSignal (CObList) "Hyg"
            22 0 1 10443 12229 0 (CStringList)          (CStringList) 1 1 1 1
            "")) (CObList) (CObList) (CObList) (CObList)    (CObList) (CObList)
                                                            (CTextView 0       
                                                             #34=(CGroupPar
            (CParagraph 0 (0 0) 1 2 0 0 180)                         
            (CObjectList                                                       
             #35=(CRefLinePar                                                  
                  (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pH7WGC2,0" 2) 5  
                         "" 0 4)                                               
                    #36=(CFolderPar                                            
                         (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178)           
                          (CObjectList                                         
                           #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)      
                                "DNA 'pH7WGC2,0'" 1)                           
                           #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180)      
                                "Currently local object. Original author: Demo
            User" 1)                  #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
            180)                           "Created: 06/24/04 08:15" 1)        
                                      #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
            180)                           "Last Modified: 09/26/07 11:24" 1)  
                                      #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
            180)                           "length: 12263 bp" 1)               
                                      #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
            180)                           "storage type: Basic" 1)            
                                      #43=(CLinePar (CParagraph 0 (0 0) 1 2 1 0
            180)                           "form: Circular" 1))) "General
            Description")            #44=(CFolderPar                           
                                          (CGroupPar (CParagraph 2 (0 0) 1 1 0
            0 178) (CObjectList))                "Standard Fields")            
                                            #45=(CFolderPar                    
                                                 (CGroupPar (CParagraph 5 (0 0)
            1 1 0 0 178)                          (CObjectList                 
                                                   #46=(CLinePar (CParagraph 0
            (0 0) 1 2 1 0 180) "Demo User"                       1))) "Original
            Author")                                 #47=(CRefLinePar          
                                                          (CLinePar (CParagraph
            0 (0 0) 0 2 0 0 233) "Comments" 2) 1 ""                0 0)        
                                                              #48=(CFolderPar  
                                                                   (CGroupPar
            (CParagraph 8 (0 0) 1 2 0 0 178) (CObjectList))               
            "Annotations")                                                
            #49=(CFolderPar                                                    
                 (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178)                  
                  (CObjectList                                                 
                   #50=(CFolderPar                                             
                        (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178)          
                         (CObjectList                                          
                          #51=(CFolderPar                                      
                               (CGroupPar                                      
                                (CParagraph 1964 (3 #24# 0) 1 2 2 0 327)       
                                   (CObjectList                                
                                    #52=(CLinePar                              
                                         (CParagraph 0 (0 0) 1 2 3 0 180)      
                                          "Start: 1964  End: 2680
            (Complementary)"                                     1))) "ECFP")))
            "CDS (1 total)")                  #53=(CFolderPar                  
                                                   (CGroupPar (CParagraph 21 (7
            21 0) 1 1 1 0 178)                        (CObjectList             
                                                       #54=(CFolderPar         
                                                            (CGroupPar         
                                                             (CParagraph 378 (3
            #26# 0) 1 2 2 0 194)                               (CObjectList    
                                                                #55=(CLinePar  
                                                                    
            (CParagraph 0 (0 0) 1 2 3 0 180)                                   
             "Start: 378   End: 1832 (Complementary)"                          
                      1))) "ccdB"))) "Misc. Feature (1 total)")                
             #56=(CFolderPar                                                   
                  (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178)              
                     (CObjectList                                              
                      #57=(CFolderPar                                          
                           (CGroupPar                                          
                            (CParagraph 8852 (3 #31# 0) 1 2 2 0 194)           
                               (CObjectList                                    
                                #58=(CLinePar                                  
                                     (CParagraph 0 (0 0) 1 2 3 0 180)          
                                      "Start: 8852  End: 10101 (Complementary)"
                                                1))) "Sm/SpR")                 
                                    #59=(CFolderPar                            
                                         (CGroupPar                            
                                          (CParagraph 10443 (3 #33# 0) 1 2 2 0
            194)                               (CObjectList                    
                                                #60=(CLinePar                  
                                                     (CParagraph 0 (0 0) 1 2 3
            0 180)                                     "Start: 10443  End:
            12229 (Complementary)"                                     1)))
            "Hyg"))) "Misc. Marker (2 total)")                  #61=(CFolderPar
                                                                     (CGroupPar
            (CParagraph 29 (7 29 0) 1 1 1 0 178)                       
            (CObjectList                                                       
             #62=(CFolderPar                                                   
                  (CGroupPar                                                   
                   (CParagraph 2702 (3 #29# 0) 1 2 2 0 194)                    
                      (CObjectList                                             
                       #63=(CLinePar                                           
                            (CParagraph 0 (0 0) 1 2 3 0 180)                   
                             "Start: 2702  End: 3729 (Complementary)"          
                                      1)                                       
                                 #64=(CLinePar                                 
                                      (CParagraph 0 (0 0) 1 2 3 0 180)         
                                       "35S promoter plus a part of omega
            leader"                                     1))) "p35S")))         
                                          "Promoter Eukaryotic (1 total)")     
                                     #65=(CFolderPar                           
                                          (CGroupPar (CParagraph 43 (7 43 0) 1
            1 1 0 178)                        (CObjectList                     
                                               #66=(CFolderPar                 
                                                    (CGroupPar                 
                                                     (CParagraph 18 (3 #28# 0)
            1 2 2 0 194)                               (CObjectList            
                                                        #67=(CLinePar          
                                                             (CParagraph 0 (0
            0) 1 2 3 0 180)                                     "Start: 18   
            End: 243 " 1))) "T35S")))                       "Terminator (1
            total)")                                        #68=(CFolderPar    
                                                                 (CGroupPar
            (CParagraph 86 (7 86 0) 1 1 1 0 178)                       
            (CObjectList                                                       
             #69=(CFolderPar                                                   
                  (CGroupPar                                                   
                   (CParagraph 253 (3 #27# 0) 1 2 2 0 194)                     
                     (CObjectList                                              
                      #70=(CLinePar                                            
                           (CParagraph 0 (0 0) 1 2 3 0 180)                    
                            "Start: 253   End: 377 " 1))) "attR2")             
                       #71=(CFolderPar                                         
                            (CGroupPar                                         
                             (CParagraph 1833 (3 #25# 0) 1 2 2 0 194)          
                                (CObjectList                                   
                                 #72=(CLinePar                                 
                                      (CParagraph 0 (0 0) 1 2 3 0 180)         
                                       "Start: 1833  End: 1957 (Complementary)"
                                                1))) "attR1")))                
                                  "Misc. Recombination (2 total)")             
                             #73=(CFolderPar                                   
                                  (CGroupPar (CParagraph 87 (7 87 0) 1 1 1 0
            178)                        (CObjectList                           
                                         #74=(CFolderPar                       
                                              (CGroupPar                       
                                               (CParagraph 3749 (3 #30# 0) 1 2
            2 0 194)                               (CObjectList                
                                                    #75=(CLinePar              
                                                         (CParagraph 0 (0 0) 1
            2 3 0 180)                                     "Start: 3749  End:
            3948" 1))) "RB")                         #76=(CFolderPar           
                                                          (CGroupPar           
                                                           (CParagraph 10107 (3
            #32# 0) 1 2 2 0 194)                               (CObjectList    
                                                                #77=(CLinePar  
                                                                    
            (CParagraph 0 (0 0) 1 2 3 0 180)                                   
             "Start: 10107  End: 10439" 1))) "LB")))                      
            "Misc. Signal (2 total)"))) "Feature Map")             
            #78=(CFolderPar                                                    
                 (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178)                  
                  (CObjectList                                                 
                   #79=(CRSFolderPar                                           
                        (CFolderPar                                            
                         (CGroupPar (CParagraph 50525264 (8 0) 1 1 1 0 178)    
                                (CObjectList                                   
                                 #80=(CGroupPar                                
                                      (CParagraph 0 (10 #0# 0) 1 2 2 0 180)    
                                      (CObjectList                             
                                       #81=(CLinePar                           
                                            (CParagraph 0 (1 #0# 1) 1 2 2 0
            191)                                     " N1: 7 " 1)              
                                                #82=(CLinePar                  
                                                     (CParagraph 0 (1 #0# 2) 1
            2 2 0 191)                                     " N2: 11911 " 1)))))
            "AatII: 2 sites") 5                       1 "GACGTC" "CTGCAG")     
                                                 #83=(CRSFolderPar             
                                                      (CFolderPar              
                                                       (CGroupPar (CParagraph
            50526224 (8 0) 1 1 1 0 178)                         (CObjectList   
                                                                 #84=(CGroupPar
                                                                     
            (CParagraph 0 (10 #1# 0) 1 2 2 0 180)                              
            (CObjectList                                                       
             #85=(CLinePar                                                     
                  (CParagraph 0 (1 #1# 1) 1 2 2 0 191)                         
                       " N1: 1 " 1))))) "ApaI: 1 site") 5 1                    
              "GGGCCC" "CCCGGG")                                            
            #86=(CRSFolderPar                                                  
                 (CFolderPar                                                   
                  (CGroupPar (CParagraph 50524544 (8 0) 1 1 1 0 178)           
                         (CObjectList                                          
                          #87=(CGroupPar                                       
                               (CParagraph 0 (10 #2# 0) 1 2 2 0 180)           
                               (CObjectList                                    
                                #88=(CLinePar                                  
                                     (CParagraph 0 (1 #2# 1) 1 2 2 0 191)      
                                          " N1: 10733 " 1))))) "AseI: 1 site")
            2 4                       "ATTAAT" "TAATTA")                       
                                 #89=(CRSFolderPar                             
                                      (CFolderPar                              
                                       (CGroupPar (CParagraph 50524784 (8 0) 1
            1 1 0 178)                         (CObjectList)) "AvaI: 8 sites")
            1 5 "CYCGRG"                       "GRGCYC")                       
                                          #90=(CRSFolderPar                    
                                               (CFolderPar                     
                                                (CGroupPar (CParagraph 50525104
            (8 0) 1 1 1 0 178)                         (CObjectList)) "BamHI: 3
            sites") 1 5 "GGATCC"                       "CCTAGG")               
                                                  #91=(CRSFolderPar            
                                                       (CFolderPar             
                                                        (CGroupPar (CParagraph
            51186288 (8 0) 1 1 1 0 178)                         (CObjectList))
            "BglII: 0 sites") 1 5 "AGATCT"                       "TCTAGA")     
                                                            #92=(CRSFolderPar  
                                                                 (CFolderPar   
                                                                  (CGroupPar
            (CParagraph 50526064 (8 0) 1 1 1 0 178)                        
            (CObjectList                                                       
             #93=(CGroupPar                                                    
                  (CParagraph 0 (10 #5# 0) 1 2 2 0 180)                        
                  (CObjectList                                                 
                   #94=(CLinePar                                               
                        (CParagraph 0 (1 #5# 1) 1 2 2 0 191)                   
                             " N1: 7003 " 1))))) "ClaI: 1 site") 2 4           
                       "ATCGAT" "TAGCTA")                                      
                  #95=(CRSFolderPar                                            
                       (CFolderPar                                             
                        (CGroupPar (CParagraph 50525424 (8 0) 1 1 1 0 178)     
                               (CObjectList                                    
                                #96=(CGroupPar                                 
                                     (CParagraph 0 (10 #6# 0) 1 2 2 0 180)     
                                     (CObjectList                              
                                      #97=(CLinePar                            
                                           (CParagraph 0 (1 #6# 1) 1 2 2 0 191)
                                                " N1: 1507 " 1))))) "EcoRI: 1
            site") 1 5                       "GAATTC" "CTTAAG")                
                                        #98=(CRSFolderPar                      
                                             (CFolderPar                       
                                              (CGroupPar (CParagraph 50524864
            (8 0) 1 1 1 0 178)                         (CObjectList)) "EcoRV: 3
            sites") 3 3 "GATATC"                       "CTATAG")               
                                                  #99=(CRSFolderPar            
                                                       (CFolderPar             
                                                        (CGroupPar (CParagraph
            50525824 (8 0) 1 1 1 0 178)                         (CObjectList   
                                                                
            #100=(CGroupPar                                                    
                  (CParagraph 0 (10 #8# 0) 1 2 2 0 180)                        
                   (CObjectList                                                
                    #101=(CLinePar                                             
                          (CParagraph 0 (1 #8# 1) 1 2 2 0 191)                 
                                 " N1: 3749 " 1))))) "HindIII: 1 site")        
                          1 5 "AAGCTT" "TTCGAA")                               
                     #102=(CRSFolderPar                                        
                           (CFolderPar                                         
                            (CGroupPar (CParagraph 50525504 (8 0) 1 1 1 0 178) 
                                    (CObjectList)) "NcoI: 3 sites") 1 5
            "CCATGG"                        "GGTACC")                          
                                      #103=(CRSFolderPar                       
                                            (CFolderPar                        
                                             (CGroupPar (CParagraph 50509168 (8
            0) 1 1 1 0 178)                          (CObjectList)) "NdeI: 4
            sites") 2 4 "CATATG"                        "GTATAC")              
                                                  #104=(CRSFolderPar           
                                                        (CFolderPar            
                                                         (CGroupPar (CParagraph
            50509088 (8 0) 1 1 1 0 178)                          (CObjectList))
            "NotI: 5 sites") 2 6 "GCGGCCGC"                        "CGCCGGCG") 
                                                             #105=(CRSFolderPar
                                                                   (CFolderPar 
                                                                    (CGroupPar
            (CParagraph 50524624 (8 0) 1 1 1 0 178)                         
            (CObjectList)) "NruI: 0 sites") 3 3 "TCGCGA"                       
            "AGCGCT")                                                    
            #106=(CRSFolderPar                                                 
                  (CFolderPar                                                  
                   (CGroupPar (CParagraph 50524944 (8 0) 1 1 1 0 178)          
                           (CObjectList)) "PstI: 3 sites") 5 1 "CTGCAG"        
                           "GACGTC")                                           
                     #107=(CRSFolderPar                                        
                           (CFolderPar                                         
                            (CGroupPar (CParagraph 51183088 (8 0) 1 1 1 0 178) 
                                    (CObjectList                               
                                     #108=(CGroupPar                           
                                           (CParagraph 0 (10 #14# 0) 1 2 2 0
            180)                                 (CObjectList                  
                                                  #109=(CLinePar               
                                                        (CParagraph 0 (1 #14#
            1) 1 2 2 0 191)                                        " N1: 3747 "
            1))))) "SacI: 1 site") 5                        1 "GAGCTC"
            "CTCGAG")                                         
            #110=(CRSFolderPar                                                 
                  (CFolderPar                                                  
                   (CGroupPar (CParagraph 51182128 (8 0) 1 1 1 0 178)          
                           (CObjectList                                        
                            #111=(CGroupPar                                    
                                  (CParagraph 0 (10 #15# 0) 1 2 2 0 180)       
                                     (CObjectList                              
                                      #112=(CLinePar                           
                                            (CParagraph 0 (1 #15# 1) 1 2 2 0
            191)                                        " N1: 245 " 1)         
                                                  #113=(CLinePar               
                                                        (CParagraph 0 (1 #15#
            2) 1 2 2 0 191)                                        " N2: 12120
            " 1))))) "SacII: 2 sites")                        4 2 "CCGCGG"
            "GGCGCC")                                        #114=(CRSFolderPar
                                                                   (CFolderPar 
                                                                    (CGroupPar
            (CParagraph 51193672 (8 0) 1 1 1 0 178)                         
            (CObjectList)) "SalI: 3 sites") 1 5 "GTCGAC"                       
            "CAGCTG")                                                    
            #115=(CRSFolderPar                                                 
                  (CFolderPar                                                  
                   (CGroupPar (CParagraph 51193752 (8 0) 1 1 1 0 178)          
                           (CObjectList                                        
                            #116=(CGroupPar                                    
                                  (CParagraph 0 (10 #17# 0) 1 2 2 0 180)       
                                     (CObjectList                              
                                      #117=(CLinePar                           
                                            (CParagraph 0 (1 #17# 1) 1 2 2 0
            191)                                        " N1: 640 " 1)         
                                                  #118=(CLinePar               
                                                        (CParagraph 0 (1 #17#
            2) 1 2 2 0 191)                                        " N2: 12245
            " 1))))) "SmaI: 2 sites")                        3 3 "CCCGGG"
            "GGGCCC")                                        #119=(CRSFolderPar
                                                                   (CFolderPar 
                                                                    (CGroupPar
            (CParagraph 51193832 (8 0) 1 1 1 0 178)                         
            (CObjectList                                                       
             #120=(CGroupPar                                                   
                   (CParagraph 0 (10 #18# 0) 1 2 2 0 180)                      
                      (CObjectList                                             
                       #121=(CLinePar                                          
                             (CParagraph 0 (1 #18# 1) 1 2 2 0 191)             
                                      " N1: 2682 " 1))))) "SpeI: 1 site") 1    
                               5 "ACTAGT" "TGATCA")                            
                         #122=(CRSFolderPar                                    
                               (CFolderPar                                     
                                (CGroupPar (CParagraph 51195512 (8 0) 1 1 1 0
            178)                          (CObjectList)) "SphI: 3 sites") 5 1
            "GCATGC"                        "CGTACG")                          
                                      #123=(CRSFolderPar                       
                                            (CFolderPar                        
                                             (CGroupPar (CParagraph 51182928 (8
            0) 1 1 1 0 178)                          (CObjectList              
                                                      #124=(CGroupPar          
                                                            (CParagraph 0 (10
            #20# 0) 1 2 2 0 180)                                 (CObjectList  
                                                                 
            #125=(CLinePar                                                     
                  (CParagraph 0 (1 #20# 1) 1 2 2 0 191)                        
                           " N1: 12254 " 1))))) "XbaI: 1 site")                
                   1 5 "TCTAGA" "AGATCT")                                      
             #126=(CRSFolderPar                                                
                   (CFolderPar                                                 
                    (CGroupPar (CParagraph 51626672 (8 0) 1 1 1 0 178)         
                            (CObjectList)) "XhoI: 0 sites") 1 5 "CTCGAG"       
                            "GAGCTC")                                          
                      #127=(CRSFolderPar                                       
                            (CFolderPar                                        
                             (CGroupPar (CParagraph 50490312 (8 0) 1 1 1 0 178)
                                     (CObjectList                              
                                      #128=(CGroupPar                          
                                            (CParagraph 0 (10 #22# 0) 1 2 2 0
            180)                                 (CObjectList                  
                                                  #129=(CLinePar               
                                                        (CParagraph 0 (1 #22#
            1) 1 2 2 0 191)                                        " N1: 638 "
            1)                                                #130=(CLinePar   
                                                                    (CParagraph
            0 (1 #22# 2) 1 2 2 0 191)                                        "
            N2: 12243 " 1))))) "XmaI: 2 sites")                        1 5
            "CCCGGG" "GGGCCC")))                                   
            "Restriction/Methylation Map:  (no more than 2 sites)"))))   
            (CGraphView                                                        
             (CWStyleSheet                                                     
              (CObjectList                                                     
               #131=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1               
                     (LOGBRUSH 0 16777215 0) 1                                 
                     (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman")
            0.8 0             1 2 "?" 0)                                       
                        #132=(CWidgetStyle "Mark 1" 0 0 1                      
                              (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings")
            0.7 0 1 2 "?"             0)                                       
                                #133=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0
            16744512) 1 0 1             (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34
            "Arial") 0.611111 8388608             1 65535 "@N (@H)" 0)         
                                            #134=(CWidgetStyle "RSite Label" 1
            (LOGPEN 0 0 8437824) 1 0 1             (LOGFONT 0 0 0 0 400 0 0 0 0
            3 2 1 18 "Times New Roman")             0.611111 0 1 5 "@N (@S)" 0)
                                              #135=(CWidgetStyle "Signal Label"
            1 (LOGPEN 0 0 4227264) 1 0 1             (LOGFONT 0 0 0 0 400 0 0 0
            0 3 2 1 34 "Arial") 0.611111 0 1 1             "@N" 0)             
                                                     #136=(CWidgetStyle
            "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1              (LOGFONT 0 0
            0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48             "@F
            bp (molecule @L bp)" 0)                                  
            #137=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1       
                  (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1    
                    "Fragment of @N" 0)                                        
              #138=(CWidgetStyle "Molecule Label 2" 0 0 1                      
                    (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New")
            0.888889 0             1 16 "@L bp" 0)                             
                             #139=(CWidgetStyle "Molecule Label 1" 0 0 1       
                                   (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34
            "Arial") 0.833333 0 1 1             "@N" 0)                        
                                          #140=(CWidgetStyle "Shape 4" 1
            (LOGPEN 0 0 0) 1 1                         (LOGBRUSH 2 8388608 5) 0
            0 0)                                 #141=(CWidgetStyle "Shape 3" 1
            (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 26367 0)             0 0 1 (LOGSHAPE
            9 1 0.8 1.8 0))                               #142=(CWidgetStyle
            "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0             0
            0)                                                         
            #143=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1                  
                  (LOGBRUSH 0 16728256 0) 0 0 1 (LOGSHAPE 8 1 0 0 0))          
            #144=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0)
            0 0             0)                                                 
                      #145=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH
            0 12632256 0)             0 0 0)                                   
                                #146=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 16384) 8
            0 0 0 1                   (LOGSHAPE 7 0.2 3.41182 2.86186
            0.609808))                    #147=(CWidgetStyle "Line 4" 1 (LOGPEN
            0 0 32768) 8 0 0 0 0)         #148=(CWidgetStyle "Line 3" 1 (LOGPEN
            0 0 16711680) 8 0 0 0 0)       #149=(CWidgetStyle "Line 2" 1
            (LOGPEN 0 0 16711680) 8 0 0 0 0)       #150=(CWidgetStyle "Line 1"
            1 (LOGPEN 0 0 16711680) 1 0 0 0 0)       #151=(CWidgetStyle "Short
            Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0)       #152=(CWidgetStyle
            "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0)              #153=(CWidgetStyle
            "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0)              #154=(CWidgetStyle
            "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0)      
            #155=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0)            
            #156=(CWidgetStyle "Void" 0 0 0 0 0)                               
            #157=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
                       (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman")
                        0.611111 128 1 5 "@N (@S)" 0)                          
                  #158=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1    
                        (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New
            Roman") 0.91 0             1 3 "@T @N " 0)                         
                                 #159=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0)
            1 0 0 0 0)           #160=(CWidgetStyle "Annotation" 0 0 1         
                                       (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18
            "Times New Roman") 0.91 0             0 0)                         
                                            #161=(CWidgetStyle "Position Label"
            1 (LOGPEN 0 0 8388608) 1 0 1             (LOGFONT 0 0 0 0 400 0 1 0
            0 3 2 1 34 "Arial") 0.63 8388608 1 1             "@N" 0)           
                                                       #162=(CWidgetStyle
            "Range" 1 (LOGPEN 0 0 0) 1 1                           (LOGBRUSH 0
            16777215 0) 0 0 0)                               
            #163=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1      
                  (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1
            1             "@N" 0)                                              
                    #164=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1  
                          (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New
            Roman")             0.611111 0 1 65535 "@N" 0)                     
                          #165=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 0) 1 1    
                                (LOGBRUSH 0 16777215 0) 1                      
                                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial")
            0.9 0 0 1             (LOGSHAPE 8 1 0 0 0))                        
                            #166=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 3342591)
            1 0 1                 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34
            "Arial") 0.666667 128 1 1             "@N" 0)                      
                                            #167=(CWidgetStyle "Shape 6" 1
            (LOGPEN 0 0 0) 1 1                         (LOGBRUSH 0 6697983 0) 1
                                                       (LOGFONT 0 0 0 0 400 0 0
            0 0 7 48 2 50 "Arial") 0.9 0 0 1             (LOGSHAPE 8 1 0 0 0)) 
                                                   #168=(CWidgetStyle "Shape 7"
            1 (LOGPEN 0 0 0) 1 1                         (LOGBRUSH 0 13421823
            0) 1                                           (LOGFONT 0 0 0 0 400
            0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)       #169=(CWidgetStyle
            "Shape 8" 1 (LOGPEN 0 0 0) 1 1                         (LOGBRUSH 0
            13434675 0) 1                                           (LOGFONT 0
            0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1            
            (LOGSHAPE 9 1 0.8 1.8 0))                                    
            #170=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 8388608) 1 1            
                  (LOGBRUSH 0 3368703 0) 1                                     
                  (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)   
               #171=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 1 1              
                     (LOGBRUSH 0 13421772 0) 1                                 
                     (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1 
                       (LOGSHAPE 8 1 0 0 0))                                   
                 #172=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 0) 1 1            
                       (LOGBRUSH 0 65535 0) 1                                  
                       (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0
            1             (LOGSHAPE 8 1 0 0 0))                                
                    #173=(CWidgetStyle "Shape 12" 1 (LOGPEN 0 0 0) 1 1         
                          (LOGBRUSH 0 3394815 0) 1                             
                          (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0
            0 1             (LOGSHAPE 8 1 0 0 0))                              
                      #174=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 3342591) 1 0 1
                            (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial")
            0.666667 128 1 1             "@N" 0)                               
                                   #175=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0
            0) 1 1                        (LOGBRUSH 0 16777164 0) 1            
                                          (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2
            50 "Arial") 0.9 0 0 0)       #176=(CWidgetStyle "Label 3" 1 (LOGPEN
            0 0 6697983) 1 0 1                 (LOGFONT 0 0 0 0 700 0 0 0 0 3 2
            1 34 "Arial") 0.666667 128 1 1             "@N" 0)                 
                                                 #177=(CWidgetStyle "Label 2" 1
            (LOGPEN 0 0 8437824) 1 0 1                 (LOGFONT 0 0 0 0 400 0 0
            0 0 3 2 1 34 "Arial") 0.611111 0 1 5             "@N (@S)" 0)      
                                                       #178=(CWidgetStyle
            "Label 6" 1 (LOGPEN 0 0 8421376) 1 0 1                 (LOGFONT 0 0
            0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 5             "@N
            (@S)" 0)                                                 
            #179=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 4227264) 1 0 1          
                  (LOGFONT 0 0 0 0 700 0 0 0 0 3 2 1 34 "Arial") 0.666667 128 1
            1             "@N" 0)                                              
                    #180=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0 8437824) 1 0 1  
                          (LOGFONT 0 0 0 0 400 255 0 0 0 3 2 1 34 "Arial")
            0.611111 0 1 5             "@N (@S)" 0)                            
                                 #181=(CWidgetStyle "Label 7" 1 (LOGPEN 0 0
            8437824) 1 0 1                 (LOGFONT 0 0 0 0 400 255 1 0 0 3 2 1
            34 "Arial") 0.611111 0 1 5             "@N (@S)" 0)                
                                             #182=(CWidgetStyle "Shape 15" 1
            (LOGPEN 0 0 6711039) 1 1                  (LOGBRUSH 0 13421823 0) 1
                                                      (LOGFONT 0 0 0 0 400 0 0
            0 0 7 48 2 50 "Arial") 0.9 0 0 0)       #183=(CWidgetStyle "Shape
            16" 1 (LOGPEN 0 0 26367) 1 1                    (LOGBRUSH 0 3394815
            0) 1                                            (LOGFONT 0 0 0 0
            400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1             (LOGSHAPE 8 1
            0 0 0))                                         #184=(CWidgetStyle
            "Shape 17" 1 (LOGPEN 0 0 102) 1 1                      (LOGBRUSH 0
            6697983 0) 1                                            (LOGFONT 0
            0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1            
            (LOGSHAPE 8 1 0 0 0))                                        
            #185=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 6710886) 1 1           
                  (LOGBRUSH 0 13421772 0) 1                                    
                  (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1    
                    (LOGSHAPE 8 1 0 0 0))                                      
              #186=(CWidgetStyle "Shape 19" 1 (LOGPEN 0 0 10066329) 1 1        
                    (LOGBRUSH 0 16777215 0) 1                                  
                    (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 1  
                      (LOGSHAPE 8 1 0 0 0))                                    
                #187=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 13260) 1 1         
                      (LOGBRUSH 0 3368703 0) 1                                 
                      (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0
            0)       #188=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 10079283) 1 1 
                           (LOGBRUSH 0 16777164 0) 1                           
                           (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9
            0 0 0)       #189=(CWidgetStyle "Shape 32" 1 (LOGPEN 0 0 6724095) 5
            1                  (LOGBRUSH 0 16777215 0) 1                       
                               (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial")
            0.9 0 0 0)       #190=(CWidgetStyle "Shape 33" 1 (LOGPEN 0 0
            10079232) 1 1                 (LOGBRUSH 0 13434777 0) 1            
                                          (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2
            50 "Arial") 0.9 0 0 1             (LOGSHAPE 9 1 0.8 1.8 0))        
                                        #191=(CWidgetStyle "Label 10" 1 (LOGPEN
            0 0 4227264) 1 0 1                (LOGFONT 0 0 0 0 700 0 0 0 0 3 2
            1 34 "Arial") 0.611111 128 1 1             "@N" 0)                 
                                                 #192=(CWidgetStyle "Shape 23"
            1 (LOGPEN 0 0 39423) 3 1                    (LOGBRUSH 0 10092543 0)
            1                                           (LOGFONT 0 0 0 0 400 0
            0 0 0 7 48 2 50 "Arial") 0.9 0 0 1             (LOGSHAPE 8 1 0 0
            0))                                         #193=(CWidgetStyle
            "Terminator" 1 (LOGPEN 0 0 0) 1 1                      (LOGBRUSH 0
            52428 0) 1                                              (LOGFONT 0
            0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0))      0.246225
            1.15807 0.164644 2.53336                                   (15
            (CShapeMapEntry 8 "Shape 22" 1 "Label 3") 4                     
            (CShapeMapEntry 0 "Shape 33" 1 "Label 0") 29                       
            (CShapeMapEntry 0 "Shape 16" 1 "Label 1") 22                       
            (CShapeMapEntry 0 "Shape 15" 1 "Label 0") 21                       
            (CShapeMapEntry 8 "Shape 17" 1 "Label 0") 43                       
            (CShapeMapEntry 8 "Shape 20" 1 "Label 0") 50                       
            (CShapeMapEntry 0 "Shape 32" 1 "Signal Label") 70                  
            (CShapeMapEntry 0 "Unique RSite" 1 "Label 7") 68                   
            (CShapeMapEntry 0 "RSite" 1 "Label 5") 67                          
            (CShapeMapEntry 0 "ORF" 0 "ORF Label") 94                          
            (CShapeMapEntry 8 "Shape 2" 1 "Label 4") 88                        
            (CShapeMapEntry 9 "Shape 11" 1 "Label 0") 87                       
            (CShapeMapEntry 8 "Shape 18" 1 "Label 0") 86                       
            (CShapeMapEntry 8 "Shape 19" 1 "Label 0") 85                       
            (CShapeMapEntry 8 "Shape 23" 1 "Label 10")) 67.6449 67.6449 39 39
            0.1      -13986) 1 1 0 1 1                                         
                    (mapper: 16.091 -17.6006 67.6449 67.6449 0.01 10 -1 12263
            12263 1 0 0)     #194=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil
            -431 100)                 (CObjectList                             
                                       #195=(CGroupWidget (CWidget 1 (0 0) 1 2
            0 0 Nil -273 100)                  (CObjectList                    
                                                #196=(CAxis                    
                                                      (CWideLine               
                                                       (CWidget 0 (0 0) 1 2 0 0
            #145# 39410724 0)                          (LOGPEN 0 2 0) 2
            (LOGBRUSH 0 12632256 0) 1 6.2747                          6.2727 1
            0.0320093) 0.0511554)                               #197=(CLabel   
                                                                      (CWidget
            1001 (0 0) 1 2 0 0 #139# 268742920 100)                        
            (LOGPEN 0 0 8437824) 1                                             
            (LOGFONT 64 24 0 0 700 0 0 0 0 3 2 1 34 "Arial")                   
                 1.15807 0.833333 0 "pH7WGC2,0" "@N" 1 0 0.579035 0            
                        -10 4.61232 0.931334 Nil)                              
                  #198=(CLabel                                                 
                        (CWidget 1002 (0 0) 1 2 0 0 #138# 31561896 100)        
                            (LOGPEN 0 0 8437824) 1                             
                            (LOGFONT 68 26 0 0 400 0 0 0 0 3 2 1 49            
                             "Courier New") 1.15807 0.888889 0 "12263 bp"      
                              "@L bp" 16 0 -0.579035 0 -10.8 2.83835 0.990466
            Nil))                  (CObjectList))                              
                             #199=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil
            -508 100)                  (CObjectList                            
                                        #200=(CGroupWidget                     
                                              (CWidget 4 (7 4 0) 1 2 0 0 Nil
            -195 100)                            (CObjectList                  
                                                  #201=(CWideArrow             
                                                        (CWideLine             
                                                         (CWidget 0 (3 #24# 0)
            1 2 0 0 #190# 0 100)                                 (LOGPEN 0 0
            10079232) 1                                             (LOGBRUSH 0
            13434777 0) 1 4.90222 5.269 1                                
            0.123113) 0.8 1.8 1)                                        
            #202=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #166# 44103204 100)                 
                          (LOGPEN 0 0 3342591) 1                               
                          (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34              
                              "Arial") 1.15807 0.666667 128 "ECFP" "@N" 1      
                                     13.0471 4.63228 0 -10 1.49309 0.783503
            #201#))                         (CObjectList))                     
                                      #203=(CGroupWidget                       
                                            (CWidget 21 (7 21 0) 1 2 0 0 Nil
            -351 100)                          (CObjectList                    
                                                #204=(CWideLine                
                                                      (CWidget 0 (3 #26# 0) 1 2
            0 0 #184# 0 100)                                (LOGPEN 0 0 102) 1
            (LOGBRUSH 0 6697983 0) 1                                5.33602
            6.08033 1 0.123113)                                   #205=(CLabel 
                                                                       
            (CWidget 0 (0 0) 1 2 0 0 #166# 568 100)                            
               (LOGPEN 0 0 3342591) 1                                          
               (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34                         
                   "Arial") 1.15807 0.666667 128 "ccdB" "@N" 1                 
                          11.2233 8.10649 0 -10 1.38961 0.783503 #204#))       
                             (CObjectList))                                    
                       #206=(CGroupWidget                                      
                             (CWidget 22 (7 22 0) 1 2 0 0 Nil -116 100)        
                             (CObjectList                                      
                              #207=(CWideArrow                                 
                                    (CWideLine                                 
                                     (CWidget 0 (3 #31# 0) 1 2 0 0 #182# 0 100)
                                            (LOGPEN 0 0 6711039) 1             
                                            (LOGBRUSH 0 13421823 0) 1 1.10598
            1.74542 1                                 0.123113) 0.8 1.8 1)     
                                               #208=(CLabel                    
                                                     (CWidget 0 (0 0) 1 2 0 0
            #166# 18088448 100)                                (LOGPEN 0 0
            3342591) 1                                              (LOGFONT 52
            19 0 0 700 0 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.666667 128 "Sm/SpR" "@N"                        
                   1 -13.9912 2.31614 0 -10 2.12876 0.783503                   
                        #207#)                                                 
                  #209=(CWideArrow                                             
                        (CWideLine                                             
                         (CWidget 0 (3 #33# 0) 1 2 0 0 #182# 0 100)            
                                (LOGPEN 0 0 6711039) 1                         
                                (LOGBRUSH 0 13421823 0) 1 0.0173928 0.93154    
                                        1 0.123113) 0.8 1.8 1)                 
                                 #210=(CLabel                                  
                                       (CWidget 0 (0 0) 1 2 0 0 #166# 4194349
            100)                                (LOGPEN 0 0 3342591) 1         
                                                (LOGFONT 52 19 0 0 700 0 0 0 0
            3 2 1 34                                 "Arial") 1.15807 0.666667
            128 "Hyg" "@N" 1                                -8.83879 10.4226 0
            -10 1.09395 0.783503 #209#))                         (CObjectList))
                                                           #211=(CGroupWidget  
                                                                 (CWidget 29 (7
            29 0) 1 2 0 0 Nil -430 100)                          (CObjectList  
                                                                 
            #212=(CWideArrow                                                   
                  (CWideLine                                                   
                   (CWidget 0 (3 #29# 0) 1 2 0 0 #183# 0 100)                  
                          (LOGPEN 0 0 26367) 1 (LOGBRUSH 0 3394815 0)          
                                  1 4.3656 4.89148 1 0.123113) 0.8 1.8 1)      
                               #213=(CLabel                                    
                                     (CWidget 0 (0 0) 1 2 0 0 #174# 268742920
            100)                                (LOGPEN 0 0 3342591) 1         
                                                (LOGFONT 52 19 0 0 700 0 0 0 0
            3 2 1 34                                 "Arial") 1.15807 0.666667
            128 "p35S" "@N" 1                                13.631 -1.15807 0
            -10 1.34526 0.783503 #212#))                         (CObjectList))
                                                           #214=(CGroupWidget  
                                                                 (CWidget 43 (7
            43 0) 1 2 0 0 Nil -351 100)                          (CObjectList  
                                                                 
            #215=(CWideLine                                                    
                  (CWidget 0 (3 #28# 0) 1 2 0 0 #187# 0 100)                   
                        (LOGPEN 0 0 13260) 1 (LOGBRUSH 0 3368703 0) 1          
                                 6.14888 6.26449 1 0.123113)                   
                           #216=(CLabel (CWidget 0 (0 0) 1 2 0 0 #166# 0 100)  
                                         (LOGPEN 0 0 3342591) 1                
                                         (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.666667 128
            "T35S" "@N" 1                                2.52263 13.8968 0 -10
            1.34526 0.783503 #215#))                         (CObjectList))    
                                                       #217=(CGroupWidget      
                                                             (CWidget 86 (7 86
            0) 1 2 0 0 Nil -586 100)                          (CObjectList     
                                                               #218=(CWideLine 
                                                                     (CWidget 0
            (3 #25# 0) 1 2 0 0 #186# 0 100)                               
            (LOGPEN 0 0 10066329) 1                                            
            (LOGBRUSH 0 16777215 0) 1 5.27207 5.33602 1                        
                   0.123113)                                                   
             #219=(CLabel                                                      
                   (CWidget 0 (0 0) 1 2 0 0 #166# 29549596 100)                
                           (LOGPEN 0 0 3342591) 1                              
                           (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34             
                               "Arial") 1.15807 0.666667 128 "attR1" "@N" 1    
                                       12.5529 5.79035 0 -10 1.43396 0.783503
            #218#)                          #220=(CWideLine                    
                                                  (CWidget 0 (3 #27# 0) 1 2 0 0
            #186# 0 100)                                (LOGPEN 0 0 10066329) 1
                                                        (LOGBRUSH 0 16777215 0)
            1 6.08033 6.14427 1                                0.123113)       
                                                         #221=(CLabel          
                                                               (CWidget 0 (0 0)
            1 2 0 0 #166# 29309008 100)                                (LOGPEN
            0 0 3342591) 1                                             
            (LOGFONT 52 19 0 0 700 0 0 0 0 3 2 1 34                            
                "Arial") 1.15807 0.666667 128 "attR2" "@N" 1                   
                        7.21346 11.5807 0 -10 1.43396 0.783503 #220#))         
                           (CObjectList))                                      
                     #222=(CGroupWidget                                        
                           (CWidget 87 (7 87 0) 1 2 0 0 Nil -195 100)          
                           (CObjectList                                        
                            #223=(CWideLine                                    
                                  (CWidget 0 (3 #30# 0) 1 2 0 0 #185# 0 100)   
                                        (LOGPEN 0 0 6710886) 1                 
                                        (LOGBRUSH 0 13421772 0) 1 4.25357
            4.35588 1                                0.123113)                 
                                               #224=(CLabel                    
                                                     (CWidget 0 (0 0) 1 2 0 0
            #166# 43302056 100)                                (LOGPEN 0 0
            3342591) 1                                              (LOGFONT 52
            19 0 0 700 0 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.666667 128 "RB" "@N" 1                          
                 12.5678 -5.79035 0 -10 0.842636 0.783503                      
                     #223#)                                                    
               #225=(CWideLine                                                 
                     (CWidget 0 (3 #32# 0) 1 2 0 0 #185# 0 100)                
                           (LOGPEN 0 0 6710886) 1                              
                           (LOGBRUSH 0 13421772 0) 1 0.933074 1.10342 1        
                                   0.123113)                                   
                             #226=(CLabel                                      
                                   (CWidget 0 (0 0) 1 2 0 0 #166# 268742920
            100)                                (LOGPEN 0 0 3342591) 1         
                                                (LOGFONT 52 19 0 0 700 0 0 0 0
            3 2 1 34                                 "Arial") 1.15807 0.666667
            128 "LB" "@N" 1                                -11.7496 6.94842 0
            -10 0.798286 0.783503                                #225#))
            (CObjectList))) (CObjectList))            #227=(CGroupWidget
            (CWidget 11 (8 0) 1 2 0 0 Nil -1056 100)                 
            (CObjectList                                                       
             #228=(CGroupWidget                                                
                   (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -743 100)                 
                   (CObjectList                                                
                    #229=(CScratch                                             
                          (CWidget 1 (1 #1# 1) 1 2 0 0 #152# 0 100)            
                               (LOGPEN 0 10 0) 8 1 6.27319 1.9 0.123113 1)     
                                #230=(CLabel                                   
                                      (CWidget 0 (0 0) 1 2 0 0 #181# 627 100)  
                                         (LOGPEN 0 0 8437824) 1                
                                         (LOGFONT 47 17 0 0 400 255 1 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0
            "{\\i Apa}I (1)"                                "@N (@S)" 5 2.15597
            16.213 0 -10 2.04007                                0.694805
            #229#)) (CObjectList))                        #231=(CGroupWidget   
                                                                (CWidget 1 (10
            #2# 0) 1 2 0 0 Nil -1996 100)                         (CObjectList 
                                                                  
            #232=(CScratch                                                     
                  (CWidget 1 (1 #2# 1) 1 2 0 0 #152# 0 100)                    
                       (LOGPEN 0 10 0) 8 1 0.783189 1.9 0.123113 1)            
                         #233=(CLabel                                          
                               (CWidget 0 (0 0) 1 2 0 0 #181# 31034776 100)    
                                       (LOGPEN 0 0 8437824) 1                  
                                       (LOGFONT 47 17 0 0 400 255 1 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Ase}I (10733)" "@N (@S)" 5
            -11.3113                                9.26456 0 -10 3.22271
            0.694805 #232#))                         (CObjectList))            
                                               #234=(CGroupWidget              
                                                     (CWidget 1 (10 #5# 0) 1 2
            0 0 Nil -586 100)                         (CObjectList             
                                                       #235=(CScratch          
                                                             (CWidget 1 (1 #5#
            1) 1 2 0 0 #152# 18088448                                 100)
            (LOGPEN 0 10 0) 8 1 2.69128 1.9                               
            0.123113 1)                                                  
            #236=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #181# 327767 100)                   
                        (LOGPEN 0 0 8437824) 1                                 
                        (LOGFONT 47 17 0 0 400 255 1 0 0 3 2 1 34              
                              "Arial") 1.15807 0.611111 0                      
                             "{\\i Cla}I (7003)" "@N (@S)" 5 -7.92567          
                                 -11.5807 0 -10 2.83835 0.694805 #235#))       
                             (CObjectList))                                    
                       #237=(CGroupWidget                                      
                             (CWidget 1 (10 #6# 0) 1 2 0 0 Nil -116 100)       
                             (CObjectList                                      
                              #238=(CScratch                                   
                                    (CWidget 1 (1 #6# 1) 1 2 0 0 #152# 1189
            100)                                (LOGPEN 0 10 0) 8 1 5.50279 1.9
            0.123113 1)                          #239=(CLabel (CWidget 0 (0 0)
            1 2 0 0 #181# 0 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 47
            17 0 0 400 255 1 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Eco}RI (1507)" "@N (@S)" 5 12.9823                           
                6.94842 0 -10 3.31141 0.694805 #238#))                        
            (CObjectList))                                               
            #240=(CGroupWidget                                                 
                  (CWidget 1 (10 #8# 0) 1 2 0 0 Nil -746 100)                  
                  (CObjectList                                                 
                   #241=(CScratch                                              
                         (CWidget 1 (1 #8# 1) 1 2 0 0 #152# 268742920          
                                  100) (LOGPEN 0 10 0) 8 1 4.35588 1.9         
                                  0.123113 1)                                  
                            #242=(CLabel                                       
                                  (CWidget 0 (0 0) 1 2 0 0 #181# 32071948 100) 
                                          (LOGPEN 0 0 8437824) 1               
                                          (LOGFONT 47 17 0 0 400 255 1 0 0 3 2
            1 34                                 "Arial") 1.15807 0.611111 0   
                                                "{\\i Hin}dIII (3749)" "@N
            (@S)" 5 14.0502                                -4.63228 0 -10
            3.42967 0.694805 #241#))                         (CObjectList))    
                                                       #243=(CGroupWidget      
                                                             (CWidget 1 (10
            #14# 0) 1 2 0 0 Nil -1608 100)                         (CObjectList
                                                                   
            #244=(CScratch                                                     
                  (CWidget 1 (1 #14# 1) 1 2 0 0 #152# 1189 100)                
                           (LOGPEN 0 10 0) 8 1 4.3569 1.9 0.123113 1)          
                           #245=(CLabel                                        
                                 (CWidget 0 (0 0) 1 2 0 0 #181# 18088584 100)  
                                         (LOGPEN 0 0 8437824) 1                
                                         (LOGFONT 47 17 0 0 400 255 1 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Sac}I (3747)" "@N (@S)" 5
            14.1037                                -3.47421 0 -10 2.92705
            0.694805 #244#))                         (CObjectList))            
                                               #246=(CGroupWidget              
                                                     (CWidget 1 (10 #18# 0) 1 2
            0 0 Nil -276 100)                         (CObjectList             
                                                       #247=(CScratch          
                                                             (CWidget 1 (1 #18#
            1) 1 2 0 0 #152# 0 100)                                (LOGPEN 0 10
            0) 8 1 4.90171 1.9 0.123113 1)                         
            #248=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #181# 32071244 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 47 17 0 0 400 255 1 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Spe}I (2682)" "@N (@S)" 5 14.324          
                                 2.31614 0 -10 2.92705 0.694805 #247#))        
                            (CObjectList))                                     
                      #249=(CGroupWidget                                       
                            (CWidget 1 (10 #20# 0) 1 2 0 0 Nil -1294 100)      
                              (CObjectList                                     
                               #250=(CScratch                                  
                                     (CWidget 1 (1 #20# 1) 1 2 0 0 #152# 1040
            100)                                (LOGPEN 0 10 0) 8 1 0.00511554
            1.9 0.123113 1)                          #251=(CLabel              
                                                           (CWidget 0 (0 0) 1 2
            0 0 #181# 327 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 47
            17 0 0 400 255 1 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Xba}I (12254)" "@N (@S)" 5 -2.70647                          
                 16.213 0 -10 3.28184 0.694805 #250#))                        
            (CObjectList))                                               
            #252=(CGroupWidget                                                 
                  (CWidget 2 (10 #0# 0) 1 2 0 0 Nil -1448 100)                 
                   (CObjectList                                                
                    #253=(CScratch                                             
                          (CWidget 1 (1 #0# 1) 1 2 0 0 #152# 762 100)          
                                 (LOGPEN 0 10 0) 8 1 6.27012 1.9 0.123113 1)   
                                  #254=(CLabel                                 
                                        (CWidget 0 (0 0) 1 2 0 0 #180# 712 100)
                                           (LOGPEN 0 0 8437824) 1              
                                           (LOGFONT 47 17 0 0 400 255 0 0 0 3 2
            1 34                                 "Arial") 1.15807 0.611111 0   
                                                "{\\i Aat}II (7)" "@N (@S)" 5
            2.12959 15.0549                                0 -10 2.04007
            0.694805 #253#)                                 #255=(CScratch     
                                                                  (CWidget 2 (1
            #0# 2) 1 2 0 0 #152# 43518548                                 100)
            (LOGPEN 0 10 0) 8 1 0.180579 1.9                               
            0.123113 1)                                                  
            #256=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #180# 268742920 100)                
                           (LOGPEN 0 0 8437824) 1                              
                           (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34           
                                 "Arial") 1.15807 0.611111 0                   
                                "{\\i Aat}II (11911)" "@N (@S)" 5 -8.1515      
                                     11.5807 0 -10 3.22271 0.694805 #255#))    
                                (CObjectList))                                 
                          #257=(CGroupWidget                                   
                                (CWidget 2 (10 #15# 0) 1 2 0 0 Nil -433 100)   
                                 (CObjectList                                  
                                  #258=(CScratch                               
                                        (CWidget 1 (1 #15# 1) 1 2 0 0 #152#
            268742952                                 100) (LOGPEN 0 10 0) 8 1
            6.14837 1.9                                0.123113 1)             
                                                 #259=(CLabel (CWidget 0 (0 0)
            1 2 0 0 #180# 0 100)                                (LOGPEN 0 0
            8437824) 1                                              (LOGFONT 47
            17 0 0 400 255 0 0 0 3 2 1 34                                
            "Arial") 1.15807 0.611111 0                                       
            "{\\i Sac}II (245)" "@N (@S)" 5 4.55829                            
               12.7388 0 -10 2.77922 0.694805 #258#)                         
            #260=(CScratch                                                     
                  (CWidget 2 (1 #15# 2) 1 2 0 0 #152# 0 100)                   
                        (LOGPEN 0 10 0) 8 1 0.0736638 1.9 0.123113 1)          
                           #261=(CLabel (CWidget 0 (0 0) 1 2 0 0 #180# 0 100)  
                                         (LOGPEN 0 0 8437824) 1                
                                         (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1
            34                                 "Arial") 1.15807 0.611111 0     
                                              "{\\i Sac}II (12120)" "@N (@S)" 5
            -4.86518                                12.7388 0 -10 3.37054
            0.694805 #260#))                         (CObjectList))            
                                               #262=(CGroupWidget              
                                                     (CWidget 2 (10 #17# 0) 1 2
            0 0 Nil -824 100)                         (CObjectList             
                                                       #263=(CScratch          
                                                             (CWidget 1 (1 #17#
            1) 1 2 0 0 #152# 44104256                                 100)
            (LOGPEN 0 10 0) 8 1 5.9463 1.9 0.123113                            
               1)                                                           
            #264=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #180# 31034108 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Sma}I (640)" "@N (@S)" 5 11.0119          
                                 9.26456 0 -10 2.77922 0.694805 #263#)         
                            #265=(CScratch                                     
                                  (CWidget 2 (1 #17# 2) 1 2 0 0 #152# 1189 100)
                                           (LOGPEN 0 10 0) 8 1 0.00971952 1.9
            0.123113 1)                          #266=(CLabel                  
                                                       (CWidget 0 (0 0) 1 2 0 0
            #180# 994 100)                                (LOGPEN 0 0 8437824)
            1                                              (LOGFONT 47 17 0 0
            400 255 0 0 0 3 2 1 34                                 "Arial")
            1.15807 0.611111 0                                        "{\\i
            Sma}I (12245)" "@N (@S)" 5 -2.79841                               
            15.0549 0 -10 3.37054 0.694805 #265#))                        
            (CObjectList))                                               
            #267=(CGroupWidget                                                 
                  (CWidget 2 (10 #22# 0) 1 2 0 0 Nil -511 100)                 
                   (CObjectList                                                
                    #268=(CScratch                                             
                          (CWidget 1 (1 #22# 1) 1 2 0 0 #152# 0 100)           
                                (LOGPEN 0 10 0) 8 1 5.94733 1.9 0.123113 1)    
                                 #269=(CLabel                                  
                                       (CWidget 0 (0 0) 1 2 0 0 #180# 29697128
            100)                                (LOGPEN 0 0 8437824) 1         
                                                (LOGFONT 47 17 0 0 400 255 0 0
            0 3 2 1 34                                 "Arial") 1.15807
            0.611111 0                                        "{\\i Xma}I
            (638)" "@N (@S)" 5 9.74209                                10.4226 0
            -10 2.77922 0.694805 #268#)                          #270=(CScratch
                                                                       (CWidget
            2 (1 #22# 2) 1 2 0 0 #152# 32071800                                
            100) (LOGPEN 0 10 0) 8 1 0.0107426 1.9                             
              0.123113 1)                                                  
            #271=(CLabel                                                       
                  (CWidget 0 (0 0) 1 2 0 0 #180# 30665756 100)                 
                          (LOGPEN 0 0 8437824) 1                               
                          (LOGFONT 47 17 0 0 400 255 0 0 0 3 2 1 34            
                                "Arial") 1.15807 0.611111 0                    
                               "{\\i Xma}I (12243)" "@N (@S)" 5 -2.74291       
                                    13.8968 0 -10 3.37054 0.694805 #270#))     
                               (CObjectList))) (CObjectList))                  
                  #272=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1059 100) 
                            (CObjectList) (CObjectList))                       
                      #273=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -668
            100)                  (CObjectList) (CObjectList))) (CObjectList)))
                    (CSeqView 10 5 (CObjectList) (CObList) 1 (CObList))
            (CObList) 0     (CStringList) 0 0 (CObList))).
FEATURES             Location/Qualifiers
     Promoter        8..282
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    283..1074
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     complement(728..747)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="598322520325420022642f94-edf1-4daf-9d42
                     -d6a0c0961135"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P7"
     rep_origin      1320..1908
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     complement(1500..1519)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="59832251447767000db70f2a-4534-4193-a589
                     -f801b3d3ae24"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P6"
     regulatory      complement(1944..2204)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /label="pBR322 bom"
     primer_bind     complement(2324..2343)
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5983225087184300f991f0bd-4d45-4ae3-ab9b
                     -e040e8fd3f94"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P5"
     rep_origin      2578..2772
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2838..3911)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     primer_bind     complement(3126..3143)
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="59832250287469009bb74542-d1bc-4535-8efb
                     -c5302a668634"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P4"
     primer_bind     complement(3929..3947)
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="5983224971182800af78c17e-6979-4e08-986d
                     -2a807688e84c"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P3"
     regulatory      complement(4340..4969)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     complement(4725..4744)
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="598322491283780060f0aadd-9fd3-44a4-8994
                     -9fb4d8cbf160"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P2"
     primer_bind     complement(5518..5536)
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="59832248543848002182b71b-f051-49ed-ab0c
                     -55cb9fc1f701"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P1"
     T-DNA_border    6155..6385
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     primer_bind     complement(6345..6364)
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5983225553607400b398d994-15dd-491f-9748
                     -3d263cd37e4e"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P14"
     promoter        6387..7433
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S - Gateway"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:3a-dlt03ty"">pCaMV 35S - Gateway</a>)"
                     /label="p35S"
     primer_bind     complement(7117..7138)
                     /%GC="45.5"
                     /Hairpin_Tm="41.9"
                     /Self_Dimer_Tm="12.2"
                     /Tm="59.9"
                     /annotation_group="5983225787179800278307c6-d034-4858-8545
                     -c7d2c79bb428"
                     /created_by="primer3"
                     /Sequence="CGCAATGATGGCATTTGTAGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P18"
     CDS             7455..8171
                     /label="ECFP"
     primer_bind     complement(7954..7973)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="5983225961020700bedeef47-6ef9-43ab-9bc4
                     -12368c49328c"
                     /created_by="primer3"
                     /Sequence="CTCGATGTTGTGGCGGATCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P33"
     attR1           8178..8302
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c-f0o2y6y"">attR1</a>"
     promoter        8327..8357
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    8411..9070
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     complement(8641..8660)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5983225671147900c5453066-0f19-4567-a1bc
                     -f5513b4a54c9"
                     /created_by="primer3"
                     /Sequence="TCACCAGCTCACCGTCTTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P16"
     UTR             9093..9411
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     CDS             9412..9717
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     primer_bind     complement(9437..9457)
                     /%GC="47.6"
                     /Hairpin_Tm="45.0"
                     /Self_Dimer_Tm="None"
                     /Tm="57.6"
                     /annotation_group="59832256120939002fa3cefe-df85-4bc2-9391
                     -7a256efb19ed"
                     /created_by="primer3"
                     /Sequence="GACGATAACGGCTCTCTCTTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P15"
     attR2           complement(9758..9882)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     terminator      9892..10117
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     primer_bind     complement(10104..10123)
                     /%GC="55.0"
                     /Hairpin_Tm="44.4"
                     /Self_Dimer_Tm="32.8"
                     /Tm="60.6"
                     /annotation_group="5983225729323500b11c41d8-a602-4616-a180
                     -346b737f8ab5"
                     /created_by="primer3"
                     /Sequence="GCCTGCAGGTCACTGGATTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P17"
     promoter        10178..10449
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="99.63%"
                     /Primary_Match="99.63%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="99.63%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     Plant_Selection 10178..11938
                     /modified_by="mamee"
                     /X-Transferred_From="Hygromycin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro
                     (<a href=""urn:local:.:l3-f0p6xgl"">Hygromycin</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Hygromycin"
                     /Transferred_Similarity="99.77%"
                     /Primary_Match="99.77%; Adjusted-Interval-0:0/0; Hygro (<a
                     href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /label="Hygro"
     CDS             10464..11486
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Hyg"
                     /Transferred_Similarity="99.71%"
                     /Primary_Match="99.71%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:90-dgycryy"">Hyg</a>"
                     /Alternative_Match="99.71%; Adjusted-Interval-0:0/0; Hyg
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /label="Hyg"
     primer_bind     complement(10681..10700)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="19.2"
                     /Tm="59.3"
                     /annotation_group="59832259036928004a0a3d36-0cf8-477a-a62d
                     -23cbbd55cfaf"
                     /created_by="primer3"
                     /Sequence="GTCAAGCACTTCCGGAATCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P23"
     primer_bind     complement(11466..11485)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.8"
                     /annotation_group="5983225845529600ea6e3a3a-0c87-43a1-b12c
                     -c2bf78344301"
                     /created_by="primer3"
                     /Sequence="TATTCCTTTGCCCTCGGACG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P22"
     terminator      11671..11938
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     T-DNA_border    11959..12291
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     complement(12203..12222)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="5983225261436200073e6862-9764-4441-8554
                     -9811b28abf4c"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pH7WGC2"
                     /label="P8"
ORIGIN      
        1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
       61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
      121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
      181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
      241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
      301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
      361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
      421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
      481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
      541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
      601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
      661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
      721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
      781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
      841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
      901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
      961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
     1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
     1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct
     1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
     3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
     3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
     3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
     3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
     3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
     3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
     3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
     3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
     3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
     3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
     3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
     4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
     4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
     4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
     4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
     4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
     4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
     4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
     4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
     4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
     4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
     4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
     4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
     4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
     4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
     4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
     4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
     4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
     5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
     5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
     5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
     5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
     5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
     5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
     5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
     5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
     5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
     5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
     5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
     5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
     5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
     5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
     5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
     5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
     6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
     6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
     6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa
     6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
     6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
     6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
     6361 atctgatcca agctcaagct aagcttgagc tctcccatat ggtcgactag agccaagctg
     6421 atctcctttg ccccggagat caccatggac gactttctct atctctacga tctaggaaga
     6481 aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa gattagcctc
     6541 ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc ggcaggtctc
     6601 atcaagacga tctacccgag taataatctc caggagatca aataccttcc caagaaggtt
     6661 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt
     6721 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg
     6781 caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa ggccatggag
     6841 tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga acagttcata
     6901 cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac
     6961 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
     7021 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
     7081 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
     7141 aaaggaaagg ctatcgttca agatgcccct gccgacagtg gtcccaaaga tggaccccca
     7201 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     7261 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     7321 ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa
     7381 ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt cgacctgcag
     7441 gcggccgcac tagtatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     7501 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     7561 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     7621 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc agccgctacc
     7681 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     7741 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     7801 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     7861 acatcctggg gcacaagctg gagtacaact acatcagcca caacgtctat atcaccgccg
     7921 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca
     7981 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     8041 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     8101 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     8161 agctgtacaa ggatatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
     8221 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     8281 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
     8341 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
     8401 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     8461 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     8521 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     8581 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     8641 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     8701 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     8761 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     8821 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     8881 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     8941 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     9001 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     9061 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     9121 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     9181 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     9241 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     9301 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     9361 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     9421 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     9481 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     9541 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     9601 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     9661 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     9721 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     9781 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     9841 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatatccc gcggccatgc
     9901 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca
     9961 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat
    10021 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
    10081 aaaatttcta attcctaaaa ccaaaatcca gtgacctgca ggcatgcgac gtcgggccct
    10141 ctagaggatc cccgggtacc ctcgaattat catacatgag aattaaggga gtcacgttat
    10201 gaccccgccg atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt
    10261 tgaaggagcc actgagccgc gggtttctgg agtttaatga gctaagcaca tacgtcagaa
    10321 accattattg cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt
    10381 caaaaatgct ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt
    10441 cactctcaac tcgaatcccc cctatgaaaa agcctgaact caccgcgacg tctgtcgaga
    10501 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
    10561 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
    10621 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
    10681 cgattccgga agtgcttgac attggggagt tcagcgagag cctgacctat tgcatctccc
    10741 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttcttc
    10801 agccggtcgc ggaggctatg gatgctatcg ctgcggccga tcttagccag acgagcgggt
    10861 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
    10921 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
    10981 ccgtcgcgca ggctctcgat gagctgatgc tttgggcgag gactgccccg aagtccggca
    11041 ctcgtgcacg cggattcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
    11101 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
    11161 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
    11221 cggagcttgc aggatcgcca aggctccggg cgtatatgct ccgcattggt cttgaccaac
    11281 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
    11341 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
    11401 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
    11461 gcactcgtcc gagggcaaag gaatagagta gatgccgacc gaacaagagc tgatttcgag
    11521 aacgcctcag ccagcaactc gcgcgagcct agcaaggcaa atgcgagaga acggccttac
    11581 gcttggtggc acagttctcg tccacagttc gctaagctcg ctcggctggg tcgcgggagg
    11641 gccggtcgca gtgattcagg aattaattcc ctagagtcaa gcagatcgtt caaacatttg
    11701 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
    11761 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
    11821 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
    11881 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacc
    11941 ggcatgcaag ctgataattc aattcggcgt taattcagta cattaaaaac gtccgcaatg
    12001 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
    12061 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
    12121 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
    12181 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
    12241 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc a
//
