LOCUS       pB7WGF2                11653 bp    DNA     circular     12-JUL-2022
DEFINITION  .
ACCESSION   urn.local...16e-dsoobj6
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     http://www.informaxinc.com/                                        
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|342085221|                                                 
            VNTDBDATE|457854157|                                               
            LSOWNER|                                                           
            VNTNAME|pB7WGF2,0|                                                 
            VNTAUTHORNAME|Demo User|                                           
            VNTAUTHOREML|manka@psb.ugent.be|                                   
            VNTOAUTHORNAME|Mansour Karimi|                                     
            VNTOAUTHORTEL|+3193313850|                                         
            VNTOAUTHOREML|mansour.karimi@psb.ugent.be|                         
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FEATURES             Location/Qualifiers
     Promoter        8..282
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    283..1074
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     complement(728..747)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="5983041083618300b63c62b3-b021-462c-81c2
                     -8ab3f21ec3da"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P7"
     rep_origin      1320..1908
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     complement(1500..1519)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="59830410261340007a1de116-cd5c-49bf-b4c4
                     -32715dc5f59b"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P6"
     regulatory      complement(1944..2204)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /label="pBR322 bom"
     primer_bind     complement(2324..2343)
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5983040968161300a05a5b9a-0623-4d57-98e2
                     -4161717698f4"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P5"
     rep_origin      2578..2772
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2838..3911)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     primer_bind     complement(3126..3143)
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="598304091016520032041314-c93e-4598-ac27
                     -ede78cba97ad"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P4"
     primer_bind     complement(3929..3947)
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="598304085091870004080e02-e02a-47e5-82f9
                     -83029d80608d"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P3"
     regulatory      complement(4340..4969)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     complement(4725..4744)
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="59830407932280009482c226-87cf-4185-8161
                     -98f5b1805ca6"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P2"
     primer_bind     complement(5518..5536)
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="598304073721190097dde938-a74c-49de-89d1
                     -aaf73e5cffc0"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P1"
     T-DNA_border    6155..6385
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     primer_bind     complement(6345..6364)
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5983041488967200ac127488-882f-420c-9cfa
                     -52cec70981ac"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P14"
     promoter        6387..7433
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S - Gateway"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:3a-dlt03ty"">pCaMV 35S - Gateway</a>)"
                     /label="p35S"
     primer_bind     complement(7117..7138)
                     /%GC="45.5"
                     /Hairpin_Tm="41.9"
                     /Self_Dimer_Tm="12.2"
                     /Tm="59.9"
                     /annotation_group="5983041719073500a02dff8d-9a51-4f2e-8005
                     -36306d4758e4"
                     /created_by="primer3"
                     /Sequence="CGCAATGATGGCATTTGTAGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P18"
     CDS             7455..8171
                     /label="Egfp"
     primer_bind     complement(7954..7973)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="5983041835498500f252dbe6-4684-4bfe-94f9
                     -500343a08512"
                     /created_by="primer3"
                     /Sequence="CTCGATGTTGTGGCGGATCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P33"
     attR1           8178..8302
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c-f0o2y6y"">attR1</a>"
     promoter        8327..8357
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    8411..9070
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     complement(8641..8660)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5983041604422900916903d8-8641-4dc8-9a6c
                     -09ae69fcaf49"
                     /created_by="primer3"
                     /Sequence="TCACCAGCTCACCGTCTTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P16"
     UTR             9093..9411
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     CDS             9412..9717
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     primer_bind     complement(9437..9457)
                     /%GC="47.6"
                     /Hairpin_Tm="45.0"
                     /Self_Dimer_Tm="None"
                     /Tm="57.6"
                     /annotation_group="59830415459466001e12bd07-01de-4308-8b84
                     -062245170509"
                     /created_by="primer3"
                     /Sequence="GACGATAACGGCTCTCTCTTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P15"
     attR2           complement(9758..9882)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     terminator      9892..10117
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     primer_bind     complement(10104..10123)
                     /%GC="55.0"
                     /Hairpin_Tm="44.4"
                     /Self_Dimer_Tm="32.8"
                     /Tm="60.6"
                     /annotation_group="598304166214050036487f9b-26ae-4e75-9015
                     -6600b921d043"
                     /created_by="primer3"
                     /Sequence="GCCTGCAGGTCACTGGATTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P17"
     promoter        10182..10454
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     Plant_Selection 10182..11300
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1k-e500sax"">Basta</a>"
                     /label="Basta"
     CDS             10475..11026
                     /%_Identity=100
                     /Motif="ATGAGCCCAGAACGACGCCCGGCCGACATCCGCCGTGCCACCGAGGCGGA
                     CATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAGACAAGCACGGTCAACTTC
                     CGTACCGAGCCGCAGGAACCGCAGGAGTGGACGGACGACCTCGTCCGTCTGCGGGAGC
                     GCTATCCCTGGCTCGTCGCCGAGGTGGACGGCGAGGTCGCCGGCATCGCCTACGCGGG
                     TCCCTGGAAGGCACGCAACGCCTACGACTGGACGGCCGAGTCGACCGTGTACGTCTCC
                     CCCCGCCACCAGCGGACGGGACTGGGCTCCACGCTCTACACCCACCTGCTGAAGTCCC
                     TGGAGGCACAGGGCTTCAAGAGCGTGGTCGCTGTCATCGGGCTGCCCAACGACCCGAG
                     CGTGCGCATGCACGAGGCGCTCGGATATGCCCCCCGCGGCATGCTGCGGGCGGCCGGC
                     TTCAAGCACGGGAACTGGCATGACGTGGGTTTCTGGCAGCTGGACTTCAGCCTGCCGG
                     TGCCGCCCCGTCCGGTCCTGCCCGTCACCGAAATCTGA"
                     /annotation_group="PAT: 8,732 -> 9,283"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PAT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:98-dgycryy"">PAT</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; PAT
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /label="PAT"
     primer_bind     complement(10790..10809)
                     /%GC="55.0"
                     /Hairpin_Tm="45.2"
                     /Self_Dimer_Tm="None"
                     /Tm="59.0"
                     /annotation_group="59830411990102007af7c3e6-cf7b-41a2-8e4d
                     -36ac472f508c"
                     /created_by="primer3"
                     /Sequence="TTCAGCAGGTGGGTGTAGAG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P9"
     terminator      11033..11300
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     T-DNA_border    11321..11653
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     complement(11565..11584)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="59830411403713001f2b223e-63cc-4057-b472
                     -8356072fe5a6"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pB7WGF2"
                     /label="P8"
ORIGIN      
        1 attcgggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
       61 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
      121 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
      181 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
      241 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatggggga agcggtgatc
      301 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
      361 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
      421 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
      481 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
      541 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
      601 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
      661 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
      721 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
      781 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
      841 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
      901 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
      961 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     1021 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataatgtcta
     1081 gctagaaatt cgttcaagcc gacgccgctt cgcggcgcgg cttaactcaa gcgttagatg
     1141 cactaagcac ataattgctc acagccaaac tatcaggtca agtctgcttt tattattttt
     1201 aagcgtgcat aataagccct acacaaattg ggagatatat catgcatgac caaaatccct
     1261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     2161 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
     2221 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     2281 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     2341 ggttttcacc gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg
     2401 tcgagtggcg acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc
     2461 atttttgagc ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc
     2521 agcgaccgaa gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat
     2581 gcacaggcca ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa
     2641 tcgccttttt tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc
     2701 tttgggttcc caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac
     2761 gtgctatcca caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct
     2821 agcatctgct ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag
     2881 cgcatgacta ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg
     2941 tcatttgacc cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg
     3001 ccactgcgtt cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg
     3061 cgtgccaggc ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga
     3121 accgtcagca cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc
     3181 tcgatctcga tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc
     3241 aaggcttcac cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc
     3301 atggcaacgt gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt
     3361 ccgccatcgg ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg
     3421 ccgggcttgt ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc
     3481 gccatcagta ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc
     3541 tctacgtgcc cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc
     3601 gacagacgga aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag
     3661 agcatcggaa cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc
     3721 gcaccggctg ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac
     3781 gattcaccgg ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc
     3841 aacttctcca ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg
     3901 cgaccgctca cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcaga
     3961 caacccagcc gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg
     4021 cgtcggtgcc tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc
     4081 tactcattta ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc
     4141 tcggtaatgg tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc
     4201 ggcaactgaa agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca
     4261 gccttgctgc tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt
     4321 ttctctttac ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga
     4381 cctcgcgggc agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag
     4441 tgcctgggta gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg
     4501 ccagcgcctc ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa
     4561 aggccgcttg tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt
     4621 cggcggtggc ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg
     4681 ccttgatcgc ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt
     4741 cgcgccggcc gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg
     4801 ttagcggttg atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat
     4861 cgactaacag aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg
     4921 tcgtcttgcc tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt
     4981 gcgtatttgt ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt
     5041 ttactcaaat acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct
     5101 ggccggccag gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac
     5161 ggttgagacg tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat
     5221 ctcttcggta atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc
     5281 tcttggcgtt cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg
     5341 aaggcaccgc gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg
     5401 tacagggtcg agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc
     5461 tggtcgatca gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca
     5521 aacttcacgc ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg
     5581 gaacgctcga actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg
     5641 gtggtgtcgg cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc
     5701 tcggcaatgt ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc
     5761 acaacgtcgc cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg
     5821 gtgccggtga tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt
     5881 tggttggtca agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg
     5941 gcgctcttgt tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga
     6001 ctaaaacacg cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact
     6061 taggacttgt gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact
     6121 gcactatagc agcggagggg ttggatcaaa gtactttaaa gtactttaaa gtactttaaa
     6181 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
     6241 aaaccttttc acgccctttt aaatatccga ttattctaat aaacgctctt ttctcttagg
     6301 tttacccgcc aatatatcct gtcaaacact gatagtttaa actgaaggcg ggaaacgaca
     6361 atctgatcca agctcaagct aagcttgagc tctcccatat ggtcgactag agccaagctg
     6421 atctcctttg ccccggagat caccatggac gactttctct atctctacga tctaggaaga
     6481 aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa gattagcctc
     6541 ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc ggcaggtctc
     6601 atcaagacga tctacccgag taataatctc caggagatca aataccttcc caagaaggtt
     6661 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt
     6721 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca taaaccaagg
     6781 caagtaatag agattggagt ctctaagaaa gtagttccta ctgaatcaaa ggccatggag
     6841 tcaaaaattc agatcgagga tctaacagaa ctcgccgtga agactggcga acagttcata
     6901 cagagtcttt tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac
     6961 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag
     7021 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
     7081 cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat
     7141 aaaggaaagg ctatcgttca agatgcccct gccgacagtg gtcccaaaga tggaccccca
     7201 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     7261 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     7321 ccttcctcta tataaggaag ttcatttcat ttggagagga ctccggtatt tttacaacaa
     7381 ttaccacaac aaaacaaaca acaaacaaca ttacaattta ctattctagt cgacctgcag
     7441 gcggccgcac tagtatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     7501 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     7561 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     7621 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     7681 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     7741 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     7801 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     7861 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     7921 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     7981 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     8041 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     8101 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     8161 agctgtacaa ggatatcaca agtttgtaca aaaaagctga acgagaaacg taaaatgata
     8221 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa
     8281 cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta cactttatgc
     8341 ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt caggagctaa
     8401 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     8461 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     8521 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     8581 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat
     8641 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     8701 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     8761 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     8821 gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga
     8881 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     8941 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     9001 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     9061 cggggcgtaa acgcgtggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
     9121 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
     9181 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
     9241 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
     9301 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
     9361 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
     9421 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
     9481 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
     9541 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
     9601 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
     9661 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
     9721 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
     9781 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     9841 atcattttac gtttctcgtt cagctttctt gtacaaagtg gtgatatccc gcggccatgc
     9901 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca
     9961 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat
    10021 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
    10081 aaaatttcta attcctaaaa ccaaaatcca gtgacctgca ggcatgcgac gtcgggccct
    10141 ctagaggatc cccgggtacc gcgaattatc gatcatgagc ggagaattaa gggagtcacg
    10201 ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc
    10261 aacgttgaag gagccactga gccgcgggtt tctggagttt aatgagctaa gcacatacgt
    10321 cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt
    10381 cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc
    10441 atattcactc tcaactcgat cgaggggatc taccatgagc ccagaacgac gcccggccga
    10501 catccgccgt gccaccgagg cggacatgcc ggcggtctgc accatcgtca accactacat
    10561 cgagacaagc acggtcaact tccgtaccga gccgcaggaa ccgcaggagt ggacggacga
    10621 cctcgtccgt ctgcgggagc gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc
    10681 cggcatcgcc tacgcgggtc cctggaaggc acgcaacgcc tacgactgga cggccgagtc
    10741 gaccgtgtac gtctcccccc gccaccagcg gacgggactg ggctccacgc tctacaccca
    10801 cctgctgaag tccctggagg cacagggctt caagagcgtg gtcgctgtca tcgggctgcc
    10861 caacgacccg agcgtgcgca tgcacgaggc gctcggatat gccccccgcg gcatgctgcg
    10921 ggcggccggc ttcaagcacg ggaactggca tgacgtgggt ttctggcagc tggacttcag
    10981 cctgccggtg ccgccccgtc cggtcctgcc cgtcaccgaa atctgatgac ccctagagtc
    11041 aagcagatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
    11101 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
    11161 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
    11221 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
    11281 catctatgtt actagatcga ccggcatgca agctgataat tcaattcggc gttaattcag
    11341 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
    11401 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
    11461 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
    11521 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
    11581 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
    11641 ctgcctgtga tca
//
