LOCUS       pP7WGF2                10500 bp    DNA     circular     12-JUL-2022
DEFINITION  .
ACCESSION   urn.local...y2-dsohm67
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     http://www.informaxinc.com/                                        
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|342084632|                                                 
            VNTDBDATE|457777119|                                               
            LSOWNER|                                                           
            VNTNAME|pP7WGF2,0|                                                 
            VNTAUTHORNAME|Demo User|                                           
            VNTAUTHOREML|manka@psb.ugent.be|                                   
            VNTOAUTHORNAME|Mansour Karimi|                                     
            VNTOAUTHORTEL|+3193313850|                                         
            VNTOAUTHOREML|mansour.karimi@psb.ugent.be|                         
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FEATURES             Location/Qualifiers
     Promoter        8..283
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="99.64%"
                     /Primary_Match="99.64%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    284..1075
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     complement(729..748)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="57076328248398008d16543a-c6aa-4dce-ba90
                     -f266dce49d47"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P7"
     rep_origin      1321..1909
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     complement(1501..1520)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="5707632765647600de8c00c3-0d29-4a5b-8f60
                     -80e2f08f9f2c"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P6"
     regulatory      complement(1945..2205)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /label="pBR322 bom"
     primer_bind     complement(2325..2344)
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5707632708004000080ada08-da20-4410-9534
                     -ca019b6824aa"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P5"
     rep_origin      2579..2773
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2839..3912)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     primer_bind     complement(3127..3144)
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="57076326491387001471137e-d2fb-4011-9be9
                     -a7d024a5e963"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P4"
     primer_bind     complement(3930..3948)
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="5707632616128500784dd274-6f4f-4a4f-9e19
                     -581230e2a57f"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P3"
     regulatory      complement(4341..4970)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     complement(4726..4745)
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5707632558425200c12a099f-cbd2-4ebb-91ea
                     -d47c4c5e86e6"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P2"
     primer_bind     complement(5519..5537)
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="5707632493632000a070c759-ee5d-4926-9991
                     -f683ddbe4bae"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P1"
     T-DNA_border    6156..6386
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     primer_bind     complement(6346..6365)
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5707633174710100a85932a0-7e66-4a41-a150
                     -22428ec6d598"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P14"
     promoter        6388..7434
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S - Gateway"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:3a-dlt03ty"">pCaMV 35S - Gateway</a>)"
                     /label="p35S"
     primer_bind     complement(7118..7139)
                     /%GC="45.5"
                     /Hairpin_Tm="41.9"
                     /Self_Dimer_Tm="12.2"
                     /Tm="59.9"
                     /annotation_group="570763340541560028ec5d7e-b5a7-46bc-aa2d
                     -cb168ac9d377"
                     /created_by="primer3"
                     /Sequence="CGCAATGATGGCATTTGTAGGT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P18"
     CDS             7456..8172
                     /label="Egfp"
     primer_bind     complement(7955..7974)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="570763347019520076f300cd-bf27-4180-9d27
                     -53694a3071af"
                     /created_by="primer3"
                     /Sequence="CTCGATGTTGTGGCGGATCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P33"
     attR1           8179..8303
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c-f0o2y6y"">attR1</a>"
     promoter        8328..8358
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    8412..9071
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     complement(8642..8661)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5707633288604700f7c24e13-e431-4f60-8599
                     -bb032c489a93"
                     /created_by="primer3"
                     /Sequence="TCACCAGCTCACCGTCTTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P16"
     UTR             9094..9412
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     primer_bind     9350..9369
                     /%GC="55.0"
                     /Hairpin_Tm="35.1"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="570763311706170025e1f47e-90be-409b-ad66
                     -8cf8f5db9472"
                     /created_by="primer3"
                     /Sequence="CTGAGGTCGCCCGGTTTATT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P13"
     CDS             9413..9718
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     primer_bind     complement(9438..9458)
                     /%GC="47.6"
                     /Hairpin_Tm="45.0"
                     /Self_Dimer_Tm="None"
                     /Tm="57.6"
                     /annotation_group="5707633231731300fbc18e13-b684-4c92-bf9d
                     -e3dafa784724"
                     /created_by="primer3"
                     /Sequence="GACGATAACGGCTCTCTCTTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P15"
     attR2           complement(9759..9883)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     terminator      9893..10118
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     T-DNA_border    10168..10500
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     complement(10412..10431)
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="5707632882424200c7ead751-63a6-4524-a11f
                     -134f4a6983b2"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pP7WGF2"
                     /label="P8"
ORIGIN      
        1 attcgggcac gaacccagtg gacataagcc tcgttcggtt cgtaagctgt aatgcaagta
       61 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
      121 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
      181 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
      241 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat
      301 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
      361 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
      421 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
      481 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
      541 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
      601 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
      661 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
      721 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
      781 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
      841 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
      901 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
      961 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     1021 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     1081 agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
     1141 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
     1201 taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc
     1261 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     1321 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     1381 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     1441 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     1501 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     1561 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1621 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1681 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1741 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1801 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     1861 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     1921 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     1981 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     2041 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     2101 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     2161 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     2221 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     2281 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     2341 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     2401 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     2461 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     2521 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     2581 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     2641 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     2701 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     2761 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     2821 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     2881 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     2941 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     3001 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     3061 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     3121 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     3181 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     3241 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     3301 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     3361 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     3421 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     3481 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     3541 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     3601 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     3661 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     3721 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     3781 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     3841 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     3901 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     3961 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     4021 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     4081 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     4141 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     4201 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     4261 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     4321 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     4381 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     4441 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     4501 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     4561 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     4621 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     4681 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     4741 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     4801 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     4861 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     4921 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     4981 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     5041 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     5101 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     5161 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     5221 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     5281 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     5341 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     5401 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     5461 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     5521 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     5581 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     5641 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     5701 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     5761 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     5821 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     5881 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     5941 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     6001 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     6061 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     6121 tgcactatag cagcggaggg gttggatcaa agtactttaa agtactttaa agtactttaa
     6181 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
     6241 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
     6301 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
     6361 aatctgatcc aagctcaagc taagcttgag ctctcccata tggtcgacta gagccaagct
     6421 gatctccttt gccccggaga tcaccatgga cgactttctc tatctctacg atctaggaag
     6481 aaagttcgac ggagaaggtg acgataccat gttcaccacc gataatgaga agattagcct
     6541 cttcaatttc agaaagaatg ctgacccaca gatggttaga gaggcctacg cggcaggtct
     6601 catcaagacg atctacccga gtaataatct ccaggagatc aaataccttc ccaagaaggt
     6661 taaagatgca gtcaaaagat tcaggactaa ctgcatcaag aacacagaga aagatatatt
     6721 tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc ataaaccaag
     6781 gcaagtaata gagattggag tctctaagaa agtagttcct actgaatcaa aggccatgga
     6841 gtcaaaaatt cagatcgagg atctaacaga actcgccgtg aagactggcg aacagttcat
     6901 acagagtctt ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga
     6961 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
     7021 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
     7081 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
     7141 taaaggaaag gctatcgttc aagatgcccc tgccgacagt ggtcccaaag atggaccccc
     7201 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     7261 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     7321 cccttcctct atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca
     7381 attaccacaa caaaacaaac aacaaacaac attacaattt actattctag tcgacctgca
     7441 ggcggccgca ctagtatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     7501 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     7561 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     7621 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     7681 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     7741 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     7801 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     7861 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     7921 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     7981 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     8041 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     8101 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     8161 gagctgtaca aggatatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat
     8221 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
     8281 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg
     8341 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
     8401 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
     8461 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
     8521 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
     8581 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
     8641 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
     8701 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
     8761 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
     8821 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
     8881 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     8941 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     9001 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     9061 gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc
     9121 gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa
     9181 gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct
     9241 caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc
     9301 cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc
     9361 cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt
     9421 taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga
     9481 tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc
     9541 agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat
     9601 gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct
     9661 cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat
     9721 gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg
     9781 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
     9841 tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgatatcc cgcggccatg
     9901 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
     9961 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
    10021 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
    10081 taaaatttct aattcctaaa accaaaatcc agtgacctgc aggcatgcga cgtcgggccc
    10141 tctagaggat ccccgggtac cgcgaattca attcggcgtt aattcagtac attaaaaacg
    10201 tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc
    10261 caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag
    10321 gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc
    10381 tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat
    10441 gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca
//
