LOCUS       pHGWFS7                13124 bp    DNA     circular     23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...1q-dnt3ece
KEYWORDS    Promoter analysis.
SOURCE      
  ORGANISM  .
COMMENT     http://www.invitrogen.com/                                         
            This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|342179951|                                                 
            VNTDBDATE|458418444|                                               
            LSOWNER|                                                           
            VNTNAME|pHGWFS7,0|                                                 
            VNTAUTHORNAME|Demo User|                                           
            VNTREPLTYPE|Plasmid                                                
            VNTEXTCHREPL|Bacteria                                              
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
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FEATURES             Location/Qualifiers
     T-DNA_border    1..333
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     primer_bind     70..89
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="56486560700881009d47768b-eb27-4da6-9d51
                     -3c86c134c659"
                     /created_by="primer3"
                     /Sequence="TCAAACCCGGCAGCTTAGTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P8"
     terminator      complement(354..621)
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="NOST"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6c-dgycryw"">NOST</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="tNOS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:38-f1t9off"">tNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; tNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="tNOS"
     Plant_Selection complement(354..2118)
                     /modified_by="mamee"
                     /X-Transferred_From="Hygromycin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro
                     (<a href=""urn:local:.:l3-f0p6xgl"">Hygromycin</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Hygromycin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Hygro
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /label="Hygro"
     CDS             complement(806..1831)
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Hyg"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:90-dgycryy"">Hyg</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; Hyg
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /label="Hyg"
     primer_bind     807..826
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.8"
                     /annotation_group="564865649353530033c75d80-8cde-4595-a426
                     -6a4b25485aa1"
                     /created_by="primer3"
                     /Sequence="TATTCCTTTGCCCTCGGACG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P22"
     primer_bind     1595..1614
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="19.2"
                     /Tm="59.3"
                     /annotation_group="564865655281790093d2ee3f-07d5-4ede-830e
                     -ab5f8f2f05db"
                     /created_by="primer3"
                     /Sequence="GTCAAGCACTTCCGGAATCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P23"
     promoter        complement(1846..2118)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pNOS"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:77-dgycryx"">pNOS</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Basta"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pNOS (<a
                     href=""urn:local:.:1k-e500sax"">Basta</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ip-f0oyhrv"">pNOS</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:lm-f0p7ifx"">Hygromycin</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; pNOS
                     (<a href=""urn:local:.:2j-e501a1r"">Kanamycin</a>)"
                     /label="pNOS"
     attR1           2230..2354
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1c-f0o2y6y"">attR1</a>"
     promoter        2379..2409
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     misc_feature    2463..3122
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     2618..2637
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.8"
                     /annotation_group="56486561302578001519c3aa-f178-420c-93c7
                     -06a004bd3ff4"
                     /created_by="primer3"
                     /Sequence="GCACAAGTTTTATCCGGCCT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P12"
     primer_bind     complement(2693..2712)
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5648656369691700b8e9a8a9-7bab-4605-bd60
                     -5ba1e3284654"
                     /created_by="primer3"
                     /Sequence="TCACCAGCTCACCGTCTTTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P16"
     UTR             3145..3463
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:.:8r-dgycryy"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     primer_bind     3401..3420
                     /%GC="55.0"
                     /Hairpin_Tm="35.1"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="56486561912857001f8ecb2a-c889-4add-b501
                     -fda4bd304092"
                     /created_by="primer3"
                     /Sequence="CTGAGGTCGCCCGGTTTATT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P13"
     CDS             3464..3769
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.67%"
                     /Primary_Match="99.67%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8r-dgycryy"">ccdB</a>"
                     /label="ccdB"
     attR2           complement(3810..3934)
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1j-f0o30z1"">attR2</a>"
     CDS             3947..4663
                     /label="Egfp"
     primer_bind     4127..4146
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.6"
                     /annotation_group="56486566096584007bf050f0-f055-450e-a2b6
                     -dfc96a0206fb"
                     /created_by="primer3"
                     /Sequence="CTCGTGACCACCCTGACCTA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P27"
     CDS             4670..6481
                     /label="gus"
     primer_bind     4759..4778
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="5648656667307100422dd59d-a59c-4312-aa5d
                     -97ef2c4a2ac8"
                     /created_by="primer3"
                     /Sequence="TGATCAGCGTTGGTGGGAAA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P30"
     primer_bind     5368..5387
                     /%GC="50.0"
                     /Hairpin_Tm="46.3"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="56486567251678004739b831-a0f4-4c16-9bc8
                     -2cea2bf79bed"
                     /created_by="primer3"
                     /Sequence="TTTGCAAGTGGTGAATCCGC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P31"
     primer_bind     5956..5975
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="14.5"
                     /Tm="60.2"
                     /annotation_group="56486567856767005588ca79-3652-4589-b23e
                     -7a6e5884ac89"
                     /created_by="primer3"
                     /Sequence="GGAAGCAACGCGTAAACTCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P32"
     terminator      6497..6722
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="t35S"
                     /Transferred_Similarity="99.56%"
                     /Primary_Match="99.56%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lk-f0ukkf4"">t35S</a>"
                     /label="t35S"
     T-DNA_border    6740..6970
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     primer_bind     6761..6780
                     /%GC="50.0"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="5648656248120100e122253b-4e92-4ac8-950b
                     -35f27253c178"
                     /created_by="primer3"
                     /Sequence="AGATTGTCGTTTCCCGCCTT"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P14"
     primer_bind     7589..7607
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.3"
                     /annotation_group="5648655657750500e77f19c3-c159-4951-84dc
                     -9d75debcb1bf"
                     /created_by="primer3"
                     /Sequence="CCCGAGGCGTGAAGTTTGG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P1"
     regulatory      8156..8785
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     primer_bind     8381..8400
                     /%GC="55.0"
                     /Hairpin_Tm="42.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="564865571976510029348498-4929-4ad6-b515
                     -e0df4af742a0"
                     /created_by="primer3"
                     /Sequence="CGCGACTTCGTAGTGATCGA"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P2"
     primer_bind     9178..9196
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="61.0"
                     /annotation_group="5648655776322700d7011551-16d2-4fed-babf
                     -078e656306e0"
                     /created_by="primer3"
                     /Sequence="CAATGGCACTGGAACCCCC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P3"
     regulatory      9214..10287
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     primer_bind     9982..9999
                     /%GC="61.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.7"
                     /Tm="60.0"
                     /annotation_group="56486558337025001ee0523f-7eda-45f3-bf53
                     -58bdda157839"
                     /created_by="primer3"
                     /Sequence="AAGAACCCGGACGTGCTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P4"
     rep_origin      10353..10547
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     primer_bind     10782..10801
                     /%GC="55.0"
                     /Hairpin_Tm="33.5"
                     /Self_Dimer_Tm="None"
                     /Tm="60.0"
                     /annotation_group="564865589324420072c7fa38-1aea-451b-a027
                     -843b80f44592"
                     /created_by="primer3"
                     /Sequence="ACCTCTGACACATGCAGCTC"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P5"
     regulatory      10921..11181
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /label="pBR322 bom"
     rep_origin      complement(11217..11805)
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer_bind     11606..11625
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.9"
                     /annotation_group="5648655949799600d35795db-1930-41cb-8575
                     -ad619bb9c146"
                     /created_by="primer3"
                     /Sequence="GCGGTGCTACAGAGTTCTTG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P6"
     misc_feature    complement(12051..12842)
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     primer_bind     12378..12397
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="5648656007627400a2e83e18-37fe-495f-b100
                     -a45a0cb6ea95"
                     /created_by="primer3"
                     /Sequence="ATCAAAGAGTTCCTCCGCCG"
                     /Mismatches=0
                     /#_Off-target_Sites=0
                     /Off-target_Template="pHGWFS7"
                     /label="P7"
     Promoter        complement(12843..13117)
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
ORIGIN      
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattatca gcttgcatgc cggtcgatct
      361 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta gggaattaat tcctgaatca ctgcgaccgg ccctcccgcg
      661 acccagccga gcgagcttag cgaactgtgg acgagaactg tgccaccaag cgtaaggccg
      721 ttctctcgca tttgccttgc taggctcgcg cgagttgctg gctgaggcgt tctcgaaatc
      781 agctcttgtt cggtcggcat ctactctatt cctttgccct cggacgagtg ctggggcgtc
      841 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
      901 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
      961 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     1021 gaccaatgcg gagcatatac gcccggagcc ttggcgatcc tgcaagctcc ggatgcctcc
     1081 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
     1141 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     1201 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
     1261 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
     1321 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
     1381 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
     1441 tcgtctggct aagatcggcc gcagcgatag catccatagc ctccgcgacc ggctgaagaa
     1501 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
     1561 tgcaataggt caggctctcg ctgaactccc caatgtcaag cacttccgga atcgggagcg
     1621 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
     1681 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
     1741 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
     1801 cagacgtcgc ggtgagttca ggctttttca taggggggat tcgagttgag agtgaatatg
     1861 agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga
     1921 aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga
     1981 cgtatgtgct tagctcatta aactccagaa acccgcggct cagtggctcc ttcaacgttg
     2041 cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac
     2101 gtgactccct taattctccg ctcatgatcg ataattcgag ctcggtaccc ggggatcctc
     2161 tagagtcgac ctgcaggcat gcaagctctc ccatatggtc gacctgcagg cggccgcact
     2221 agtgatatca caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     2281 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     2341 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
     2401 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     2461 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     2521 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     2581 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     2641 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     2701 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     2761 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     2821 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     2881 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     2941 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     3001 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     3061 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
     3121 aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     3181 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     3241 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     3301 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     3361 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     3421 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     3481 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     3541 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     3601 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     3661 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     3721 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     3781 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
     3841 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     3901 acgtttctcg ttcagctttc ttgtacaaag tggtgatatc ccgcggatgg tgagcaaggg
     3961 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     4021 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     4081 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     4141 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     4201 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     4261 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     4321 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     4381 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     4441 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     4501 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     4561 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     4621 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagcccggca tgttacgtcc
     4681 tgtagaaacc ccaacccgtg aaatcaaaaa actcgacggc ctgtgggcat tcagtctgga
     4741 tcgcgaaaac tgtggaattg atcagcgttg gtgggaaagc gcgttacaag aaagccgggc
     4801 aattgctgtg ccaggcagtt ttaacgatca gttcgccgat gcagattttc gtaattatgc
     4861 gggcaacgtc tggtatcagc gcgaagtctt tataccgaaa ggttgggcag gccagcgtat
     4921 cgtgctgcgt ttcgatgcgg tcactcatta cggcaaagtg tgggtcaata atcaggaagt
     4981 gatggagcat cagggcggct atacgccatt tgaagccgat gtcacgccgt atgttattgc
     5041 cgggaaaagt gtacgtatca ccgtttgcgt gaacaacgaa ctgaactggc agactatccc
     5101 gccgggaatg gtgattaccg acgaaaacgg caagaaaaag cagtcttact tccatgattt
     5161 ctttaactat gccggaatcc atcgcagcgt aatgctctac accacgccga acacctgggt
     5221 ggacgatatc accgtggtga cgcatgtcgc gcaagactgt aaccacgcgt ctgttgactg
     5281 gcaggtggtg gccaatggtg atgtcagcgt tgaactgcgt gatgcggatc aacaggtggt
     5341 tgcaactgga caaggcacta gcgggacttt gcaagtggtg aatccgcacc tctggcaacc
     5401 gggtgaaggt tatctctatg aactgtgcgt cacagccaaa agccagacag agtgtgatat
     5461 ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag ggcgaacagt tcctgattaa
     5521 ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa gatgcggact tgcgtggcaa
     5581 aggattcgat aacgtgctga tggtgcacga ccacgcatta atggactgga ttggggccaa
     5641 ctcctaccgt acctcgcatt acccttacgc tgaagagatg ctcgactggg cagatgaaca
     5701 tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt aacctctctt taggcattgg
     5761 tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa gaggcagtca acggggaaac
     5821 tcagcaagcg cacttacagg cgattaaaga gctgatagcg cgtgacaaaa accacccaag
     5881 cgtggtgatg tggagtattg ccaacgaacc ggatacccgt ccgcaaggtg cacgggaata
     5941 tttcgcgcca ctggcggaag caacgcgtaa actcgacccg acgcgtccga tcacctgcgt
     6001 caatgtaatg ttctgcgacg ctcacaccga taccatcagc gatctctttg atgtgctgtg
     6061 cctgaaccgt tattacggat ggtatgtcca aagcggcgat ttggaaacgg cagagaaggt
     6121 actggaaaaa gaacttctgg cctggcagga gaaactgcat cagccgatta tcatcaccga
     6181 atacggcgtg gatacgttag ccgggctgca ctcaatgtac accgacatgt ggagtgaaga
     6241 gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca gcgccgtcgt
     6301 cggtgaacag gtatggaatt tcgccgattt tgcgacctcg caaggcatat tgcgcgttgg
     6361 cggtaacaag aaagggatct tcactcgcga ccgcaaaccg aagtcggcgg cttttctgct
     6421 gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg cagcagggag gcaaacaatg
     6481 accatggcgg ccgggagggc catgctagag tccgcaaaaa tcaccagtct ctctctacaa
     6541 atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt
     6601 agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     6661 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac
     6721 ctgcaggcat gcgacgtcga gcttagcttg agcttggatc agattgtcgt ttcccgcctt
     6781 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     6841 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     6901 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttaaa gtactttaaa
     6961 gtactttaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
     7021 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct
     7081 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat
     7141 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg
     7201 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac
     7261 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg
     7321 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta
     7381 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
     7441 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
     7501 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
     7561 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
     7621 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
     7681 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
     7741 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
     7801 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
     7861 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
     7921 tcgcggccgg gtacgtgttc gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa
     7981 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
     8041 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
     8101 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
     8161 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
     8221 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
     8281 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
     8341 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
     8401 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
     8461 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
     8521 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
     8581 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
     8641 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
     8701 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
     8761 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
     8821 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
     8881 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
     8941 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
     9001 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
     9061 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
     9121 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa
     9181 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg
     9241 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
     9301 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
     9361 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag
     9421 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
     9481 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
     9541 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
     9601 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
     9661 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
     9721 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
     9781 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
     9841 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
     9901 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
     9961 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
    10021 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
    10081 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
    10141 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
    10201 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
    10261 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
    10321 gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca
    10381 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
    10441 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
    10501 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
    10561 cgcacagccg aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc
    10621 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca
    10681 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat
    10741 caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    10801 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    10861 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
    10921 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    10981 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
    11041 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    11101 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    11161 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    11221 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    11281 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    11341 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    11401 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    11461 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    11521 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    11581 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    11641 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11701 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11761 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11821 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11881 catgatatat ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca
    11941 gacttgacct gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta acgcttgagt
    12001 taagccgcgc cgcgaagcgg cgtcggcttg aacgaatttc tagctagaca ttatttgccg
    12061 actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc
    12121 gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc
    12181 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
    12241 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
    12301 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
    12361 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
    12421 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
    12481 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
    12541 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
    12601 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
    12661 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
    12721 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
    12781 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccccc
    12841 atgatgttta actttgtttt agggcgactg ccctgctgcg taacatcgtt gctgctccat
    12901 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
    12961 tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
    13021 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagc
    13081 ttacgaaccg aacaggctta tgtccactgg gttcgtgccc gaat
//
