LOCUS       pBbm42GW7-pOsU6-sgRNA(VYL)-pZmUBIL-Cas9PTA-G7T       15901 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...lt-eqt4mz6
VERSION     urn.local...lt-eqt4mz6
KEYWORDS    .
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct.
FEATURES             Location/Qualifiers
     T-DNA_border    1..333
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
     terminator      complement(352..621)
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="NOST"
                     /Transferred_Similarity="99.26%"
                     /Primary_Match="99.26%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1c-c4nnd54"">NOST</a>"
                     /label="NOST"
     Plant_Selection complement(352..2107)
                     /created_by="mamee"
                     /label="Basta"
     CDS             complement(628..1179)
                     /%_Identity=100
                     /Motif="ATGAGCCCAGAACGACGCCCGGCCGACATCCGCCGTGCCACCGAGGCGGA
                     CATGCCGGCGGTCTGCACCATCGTCAACCACTACATCGAGACAAGCACGGTCAACTTC
                     CGTACCGAGCCGCAGGAACCGCAGGAGTGGACGGACGACCTCGTCCGTCTGCGGGAGC
                     GCTATCCCTGGCTCGTCGCCGAGGTGGACGGCGAGGTCGCCGGCATCGCCTACGCGGG
                     TCCCTGGAAGGCACGCAACGCCTACGACTGGACGGCCGAGTCGACCGTGTACGTCTCC
                     CCCCGCCACCAGCGGACGGGACTGGGCTCCACGCTCTACACCCACCTGCTGAAGTCCC
                     TGGAGGCACAGGGCTTCAAGAGCGTGGTCGCTGTCATCGGGCTGCCCAACGACCCGAG
                     CGTGCGCATGCACGAGGCGCTCGGATATGCCCCCCGCGGCATGCTGCGGGCGGCCGGC
                     TTCAAGCACGGGAACTGGCATGACGTGGGTTTCTGGCAGCTGGACTTCAGCCTGCCGG
                     TGCCGCCCCGTCCGGTCCTGCCCGTCACCGAAATCTGA"
                     /annotation_group="PAT: 8,732 -> 9,283"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PAT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:3h-c4nnd56"">PAT</a>"
                     /label="PAT"
     promoter        complement(1244..2107)
                     /regulatory_class="promoter"
                     /gene="CaMV 35S"
                     /note="internal BsaI recognition site removed"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S"
                     /Transferred_Similarity="93.06%"
                     /Primary_Match="93.06%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1n-c4nnd54"">pCaMV 35S</a>"
                     /label="pCaMV 35S"
     promoter        2266..4252
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:thjac:1m-c4nnd54"">pZmUBIL</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/-1008;
                     ZmUBIL.2 (<a
                     href=""urn:local:lapau:v-c52za60"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     misc_feature    3072
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     intron          3244..4253
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a
                     href=""urn:local:lapau:v-c52za60"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     CDS             4287..8402
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Cas9PTA"
                     /Transferred_Similarity="100.00%"
                     /Adjusted_Transferred_Similarity="99.13%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/-36
                     (adjusted to 99.13%; Adjusted-Interval-0:0/0); CAS9 (<a
                     href=""urn:local:thjac:3c-c4nnd56"">Cas9PTA</a>)"
                     /Alternative_Match="99.98%; Adjusted-Interval-0:0/-36
                     (adjusted to 99.11%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:thjac:pe-ecpo7bv"">nCAS9(D10A)</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:3/-36
                     (adjusted to 99.06%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:warddecaestecker:2q-c8811q6"">SpCas9</a>"
                     /Alternative_Match="99.93%; Adjusted-Interval-0:3/-36
                     (adjusted to 98.99%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:warddecaestecker:2p-c8811q6"">SpCas9(D10A
                     )</a>"
                     /Alternative_Match="99.93%; Adjusted-Interval-0:3/-36
                     (adjusted to 98.99%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:warddecaestecker:2o-c8811q6"">SpCas9(H840
                     A)</a>"
                     /Alternative_Match="99.85%; Adjusted-Interval-0:3/-36
                     (adjusted to 98.91%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:warddecaestecker:2s-c8811q6"">SpCas9(D10A
                     ,H840A)</a>"
                     /label="CAS9"
     NLS             8403..8423
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     Linker          8431..8476
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1j-c4nnd54"">Linker</a>"
                     /label="Linker"
     terminator      8481..8752
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G7T"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1a-c4nnd53"">G7T</a>"
                     /label="G7T"
     promoter        8826..9071
                     /created_by="lapau"
                     /label="OsU6-2"
     Spacer          9072..9091
                     /created_by="lapau"
                     /label="VYL"
     Scaffold        9092..9174
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpCas9 Scaffold"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
                     (<a href=""urn:local:thjac:2f-c4nnd55"">SpCas9
                     Scaffold</a>)"
                     /label="Scaffold"
     terminator      9281..9520
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="35ST"
                     /Transferred_Similarity="95.00%"
                     /Primary_Match="95.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1d-c4nnd54"">35ST</a>"
                     /label="35ST"
     T-DNA_border    9544..9773
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="91.77%"
                     /Primary_Match="91.77%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     regulatory      10929..11558
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7d-dlucaql"">pVS1 staA</a>"
                     /label="pVS1 staA"
     regulatory      11987..13059
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7b-dlucaql"">pVS1 repA</a>"
                     /label="pVS1 repA"
     rep_origin      13125..13319
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      13693..13953
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150;
                     <a href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      complement(13989..14577)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    complement(14647..15896)
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="98.72%"
                     /Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2r-c4nnd56"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
ORIGIN      
        1 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
       61 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
      121 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
      181 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
      241 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
      301 cgtttttaat gtactgaatt aacgccgaat tgaattcagc ttgcatgccg gtcgatctag
      361 taacatatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
      421 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
      481 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
      541 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
      601 gaacgatctg cttgactcta ggggtcatca gatttcggtg acgggcagga ccggacgggg
      661 cggcaccggc aggctgaagt ccagctgcca gaaacccacg tcatgccagt tcccgtgctt
      721 gaagccggcc gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac
      781 gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc ttgaagccct gtgcctccag
      841 ggacttcagc aggtgggtgt agagcgtgga gcccagtccc gtccgctggt ggcgggggga
      901 gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc agggacccgc
      961 gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg agccagggat agcgctcccg
     1021 cagacggacg aggtcgtccg tccactcctg cggttcctgc ggctcggtac ggaagttgac
     1081 cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt
     1141 ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatc tgcaggtcga ctagaatagt
     1201 aaattgtaat gttgtttgtt gtttgttttg ttgtggtatt gttgtaaaaa taccggagtc
     1261 ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg atagtgggat
     1321 tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt tgaagacgtg
     1381 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
     1441 ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg gcatttgtag
     1501 gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg gcaatggaat
     1561 ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct ttggtcttct
     1621 gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac catgttgacg
     1681 aagattttct tcttgtcatt gagtcgtaaa agactctgta tgaactgttc gccagtcttc
     1741 acggcgagtt ctgttagatc ctcgatctga atttttgact ccatggcctt tgattcagta
     1801 ggaactactt tcttagagac tccaatctct attacttgcc ttggtttatg aagcaagcct
     1861 tgaatcgtcc atactggaat agtacttctg atcttgagaa atatatcttt ctctgtgttc
     1921 ttgatgcagt tagtcctgaa tcttttgact gcatctttaa ccttcttggg aaggtatttg
     1981 atctcctgga gattattact cgggtagatc gtcttgatga gacctgccgc gtaggcctct
     2041 ctaaccatct gtgggtcagc attctttctg aaattgaaga ggctaatctt ctcattatcg
     2101 gtggtgaaca tggtatcgtc accttctccg tcgaactttc ttcctagatc gtagagatag
     2161 agaaagtcgt ccatggtgat ctccggggca aaggagatca gcttggctct agtcgaccat
     2221 atgggagaat tcgagctcca actttgtata gaaaagttgc tacctgtgca gcgtgacccg
     2281 gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca
     2341 tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa
     2401 ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc
     2461 atataaatga acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc
     2521 tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct
     2581 atataatact tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt
     2641 atagactaat ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac
     2701 taaaactcta ttttagtttt tttatttaat aatttagata taaaatagaa taaaataaag
     2761 tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct
     2821 tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac
     2881 cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc
     2941 tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat
     3001 ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc
     3061 tctcacggca ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc
     3121 tcgcccgccg taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt
     3181 cggagcgcac acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc
     3241 aaggtacgcc gctcgtcctc cccccccccc cctctctacc ttctctagat cggcgttccg
     3301 gtccatggtt agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg
     3361 tgttagatcc gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct
     3421 gattgctaac ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc
     3481 agacgggatc gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt
     3541 cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt
     3601 gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattctg
     3661 tttcaaacta cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc
     3721 atagttacga attgaagatg atggatggaa atatcgatct aggataggta tacatgttga
     3781 tgcgggtttt actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg
     3841 gtgtggttgg gcggtcgttc attcgttcta gatcggagta gaatactgtt tcaaactacc
     3901 tggtgtattt attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt
     3961 ttaagatgga tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga
     4021 tgcatataca tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc
     4081 tattataata aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc
     4141 atatgcagca gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt
     4201 ggtactgttt cttttgtcga tgctcaccct gttgtttggt gttacttctg caaacagtat
     4261 tcagtcgact ggtaccaaca ggctccatgg ataagaagta ctctatcgga ctcgatatcg
     4321 gaactaactc tgtgggatgg gctgtgatca ccgatgagta caaggtgcca tctaagaagt
     4381 tcaaggttct cggaaacacc gataggcact ctatcaagaa aaaccttatc ggtgctctcc
     4441 tcttcgattc tggtgaaact gctgaggcta ccagactcaa gagaaccgct agaagaaggt
     4501 acaccagaag aaagaacagg atctgctacc tccaagagat cttctctaac gagatggcta
     4561 aagtggatga ttcattcttc cacaggctcg aagagtcatt cctcgtggaa gaagataaga
     4621 agcacgagag gcaccctatc ttcggaaaca tcgttgatga ggtggcatac cacgagaagt
     4681 accctactat ctaccacctc agaaagaagc tcgttgattc tactgataag gctgatctca
     4741 ggctcatcta cctcgctctc gctcacatga tcaagttcag aggacacttc ctcatcgagg
     4801 gtgatctcaa ccctgataac tctgatgtgg ataagttgtt catccagctc gtgcagacct
     4861 acaaccagct tttcgaagag aaccctatca acgcttcagg tgtggatgct aaggctatcc
     4921 tctctgctag gctctctaag tcaagaaggc ttgagaacct cattgctcag ctccctggtg
     4981 agaagaagaa cggacttttc ggaaacttga tcgctctctc tctcggactc acccctaact
     5041 tcaagtctaa cttcgatctc gctgaggatg caaagctcca gctctcaaag gatacctacg
     5101 atgatgatct cgataacctc ctcgctcaga tcggagatca gtacgctgat ttgttcctcg
     5161 ctgctaagaa cctctctgat gctatcctcc tcagtgatat cctcagagtg aacaccgaga
     5221 tcaccaaggc tccactctca gcttctatga tcaagagata cgatgagcac caccaggatc
     5281 tcacacttct caaggctctt gttagacagc agctcccaga gaagtacaaa gagattttct
     5341 tcgatcagtc taagaacgga tacgctggtt acatcgatgg tggtgcatct caagaagagt
     5401 tctacaagtt catcaagcct atcctcgaga agatggatgg aaccgaggaa ctcctcgtga
     5461 agctcaatag agaggatctt ctcagaaagc agaggacctt cgataacgga tctatccctc
     5521 atcagatcca cctcggagag ttgcacgcta tccttagaag gcaagaggat ttctacccat
     5581 tcctcaagga taacagggaa aagattgaga agattctcac cttcagaatc ccttactacg
     5641 tgggacctct cgctagagga aactcaagat tcgcttggat gaccagaaag tctgaggaaa
     5701 ccatcacccc ttggaacttc gaagaggtgg tggataaggg tgctagtgct cagtctttca
     5761 tcgagaggat gaccaacttc gataagaacc ttccaaacga gaaggtgctc cctaagcact
     5821 ctttgctcta cgagtacttc accgtgtaca acgagttgac caaggttaag tacgtgaccg
     5881 agggaatgag gaagcctgct tttttgtcag gtgagcaaaa gaaggctatc gttgatctct
     5941 tgttcaagac caacagaaag gtgaccgtga agcagctcaa agaggattac ttcaagaaaa
     6001 tcgagtgctt cgattcagtt gagatttctg gtgttgagga taggttcaac gcatctctcg
     6061 gaacctacca cgatctcctc aagatcatta aggataagga tttcttggat aacgaggaaa
     6121 acgaggatat cttggaggat atcgttctta ccctcaccct ctttgaagat agagagatga
     6181 ttgaagaaag gctcaagacc tacgctcatc tcttcgatga taaggtgatg aagcagttga
     6241 agagaagaag atacactggt tggggaaggc tctcaagaaa gctcattaac ggaatcaggg
     6301 ataagcagtc tggaaagaca atccttgatt tcctcaagtc tgatggattc gctaacagaa
     6361 acttcatgca gctcatccac gatgattctc tcacctttaa agaggatatc cagaaggctc
     6421 aggtttcagg acagggtgat agtctccatg agcatatcgc taacctcgct ggatctcctg
     6481 caatcaagaa gggaatcctc cagactgtga aggttgtgga tgagttggtg aaggtgatgg
     6541 gaaggcataa gcctgagaac atcgtgatcg aaatggctag agagaaccag accactcaga
     6601 agggacagaa gaactctagg gaaaggatga agaggatcga ggaaggtatc aaagagcttg
     6661 gatctcagat cctcaaagag caccctgttg agaacactca gctccagaat gagaagctct
     6721 acctctacta cctccagaac ggaagggata tgtatgtgga tcaagagttg gatatcaaca
     6781 ggctctctga ttacgatgtt gatcatatcg tgccacagtc attcttgaag gatgattcta
     6841 tcgataacaa ggtgctcacc aggtctgata agaacagggg taagagtgat aacgtgccaa
     6901 gtgaagaggt tgtgaagaaa atgaagaact attggaggca gctcctcaac gctaagctca
     6961 tcactcagag aaagttcgat aacttgacta aggctgagag gggaggactc tctgaattgg
     7021 ataaggcagg attcatcaag aggcagcttg tggaaaccag gcagatcact aagcacgttg
     7081 cacagatcct cgattctagg atgaacacca agtacgatga gaacgataag ttgatcaggg
     7141 aagtgaaggt tatcaccctc aagtcaaagc tcgtgtctga tttcagaaag gatttccaat
     7201 tctacaaggt gagggaaatc aacaactacc accacgctca cgatgcttac cttaacgctg
     7261 ttgttggaac cgctctcatc aagaagtatc ctaagctcga gtcagagttc gtgtacggtg
     7321 attacaaggt gtacgatgtg aggaagatga tcgctaagtc tgagcaagag atcggaaagg
     7381 ctaccgctaa gtatttcttc tactctaaca tcatgaattt cttcaagacc gagattaccc
     7441 tcgctaacgg tgagatcaga aagaggccac tcatcgagac aaacggtgaa acaggtgaga
     7501 tcgtgtggga taagggaagg gatttcgcta ccgttagaaa ggtgctctct atgccacagg
     7561 tgaacatcgt taagaaaacc gaggtgcaga ccggtggatt ctctaaagag tctatcctcc
     7621 ctaagaggaa ctctgataag ctcattgcta ggaagaagga ttgggaccct aagaaatacg
     7681 gtggtttcga ttctcctacc gtggcttact ctgttctcgt tgtggctaag gttgagaagg
     7741 gaaagagtaa gaagctcaag tctgttaagg aacttctcgg aatcactatc atggaaaggt
     7801 catctttcga gaagaaccca atcgatttcc tcgaggctaa gggatacaaa gaggttaaga
     7861 aggatctcat catcaagctc ccaaagtact cactcttcga actcgagaac ggtagaaaga
     7921 ggatgctcgc ttctgctggt gagcttcaaa agggaaacga gcttgctctc ccatctaagt
     7981 acgttaactt tctttacctc gcttctcact acgagaagtt gaagggatct ccagaagata
     8041 acgagcagaa gcaacttttc gttgagcagc acaagcacta cttggatgag atcatcgagc
     8101 agatctctga gttctctaaa agggtgatcc tcgctgatgc aaacctcgat aaggtgttgt
     8161 ctgcttacaa caagcacaga gataagccta tcagggaaca ggcagagaac atcatccatc
     8221 tcttcaccct taccaacctc ggtgctcctg ctgctttcaa gtacttcgat acaaccatcg
     8281 ataggaagag atacacctct accaaagaag tgctcgatgc taccctcatc catcagtcta
     8341 tcactggact ctacgagact aggatcgatc tctcacagct cggtggtgat tcaagggctg
     8401 atcctaagaa gaagaggaag gtttgatcag gtggatccta gataaccttt accttcattc
     8461 cctgtaaatt ggtaccctgc ccccgatgag ctaagctagc tatatcatca atttatgtat
     8521 tacacataat atcgcactca gtctttcatc tacggcaatg taccagctga tataatcagt
     8581 tattgaaata tttctgaatt taaacttgca tcaataaatt tatgtttttg cttggactat
     8641 aatacctgac ttgttatttt atcaataaat atttaaacta tatttctttc aagatgggaa
     8701 ttaacatcta caaattgcct tttcttatcg accatgtact cgacggccga gtactaatag
     8761 aattcgatat tatttagtat gcaagtttgt acaaaaaagc aggctccgcg gccgccccct
     8821 tcaccggatc atgaaccaac ggcctggctg tatttggtgg ttgtgtaggg agatggggag
     8881 aagaaaagcc cgattctctt cgctgtgatg ggctggatgc atgcggggga gcgggaggcc
     8941 caagtacgtg cacggtgagc ggcccacagg gcgagtgtga gcgcgagagg cgggaggaac
     9001 agtttagtac cacattgccc agctaactcg aacgcgacca acttataaac ccgcgcgctg
     9061 tcgcttgtgt ttcggtgcgg aggccctgtt ggttttagag ctagaaatag caagttaaaa
     9121 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgtccct
     9181 tcgaagggca attcccaagg gtgggcgcgc cgacccagct ttcttgtaca aagtggtgat
     9241 atcccgcggc catggcggcc gggagcatgc gacgtcgatc taactgacta gccgcggcca
     9301 tgctagagtc cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct
     9361 ccagaataat gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct
     9421 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
     9481 aataaaattt ctaattccta aaaccaaaat ccagtgacct gcaggcatgc gacgtcgggc
     9541 ccaagcttag cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag
     9601 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg
     9661 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     9721 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     9781 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     9841 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     9901 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     9961 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
    10021 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
    10081 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
    10141 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
    10201 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
    10261 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
    10321 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
    10381 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
    10441 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
    10501 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
    10561 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
    10621 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
    10681 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
    10741 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
    10801 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
    10861 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
    10921 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
    10981 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
    11041 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
    11101 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
    11161 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
    11221 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
    11281 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
    11341 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
    11401 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
    11461 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
    11521 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
    11581 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
    11641 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
    11701 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
    11761 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
    11821 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
    11881 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
    11941 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa
    12001 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
    12061 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
    12121 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
    12181 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
    12241 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
    12301 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
    12361 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
    12421 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
    12481 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
    12541 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
    12601 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
    12661 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
    12721 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
    12781 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
    12841 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
    12901 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
    12961 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
    13021 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
    13081 caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac
    13141 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
    13201 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
    13261 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
    13321 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
    13381 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
    13441 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
    13501 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
    13561 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    13621 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
    13681 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
    13741 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    13801 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    13861 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    13921 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    13981 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    14041 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    14101 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    14161 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    14221 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    14281 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    14341 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    14401 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
    14461 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    14521 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    14581 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    14641 attttggtca tgcatgatat atctcccaat ttgtgtaggg cttattatgc acgcttaaaa
    14701 ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc
    14761 taatcgcttg agttaacgcc ggcgaagcgg cgtcggcttg aacgaatttc tagctagaca
    14821 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
    14881 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
    14941 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
    15001 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
    15061 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
    15121 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
    15181 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
    15241 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
    15301 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
    15361 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
    15421 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
    15481 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
    15541 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
    15601 cgcttccccc atgatgttta actttgtttt agggcgactg ccctgctgcg taacatcgtt
    15661 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
    15721 aggcatagac tgtaccccaa aaaaacatgt cataacaaga agccatgaaa accgccactg
    15781 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgac ggcagttacg
    15841 ctacttgcat tacagcttac gaaccgaacg aggcttatgt ccactgggtt cgtgcccgaa
    15901 t
//
