LOCUS       pGGIB-U5-LoxP-mRUBY3-tocs        4016 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...93-aruixik
VERSION     urn.local...93-aruixik
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_difference 1..18
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     misc_feature    19..58
                     /created_by="lapau"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U5"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:6b-cctbnue"">U5</a>"
                     /label="U5"
                     /note="Geneious type: Golden Gibson Cloning site"
     motif           95..128
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="loxP"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:8a-c541szf"">loxP</a>"
                     /label="LoxP"
     CDS             132..842
                     /note="added by Rabuo"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mRuby3"
                     /Transferred_Similarity="99.58%"
                     /Primary_Match="99.58%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:3g-c4nnd56"">mRuby3</a>"
                     /label="mRuby3"
     terminator      847..1613
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="OCST"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:8f-c542jqg"">OCST</a>"
                     /label="OCST"
     misc_feature    1624..1663
                     /created_by="lapau"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U6"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:6d-cctbnug"">U6</a>"
                     /label="U6"
                     /note="Geneious type: Golden Gibson Cloning site"
     misc_difference complement(1664..1681)
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     primer          complement(1709..1729)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        1790..1840
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    1841..2650
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2o-c4nnd56"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2797..3385
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3985..4005
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 attaccctgt tatccctacc aggatacata gattaccaca actccgagcc cttccaccct
       61 cgagatacct tcagcctgat cctgagcttc tagcataact tcgtatagca tacattatac
      121 gaagttatgg ctccgtgtct aagggcgaag agctgatcaa ggaaaatatg cgtatgaagg
      181 tggtcatgga aggttcggtc aacggccacc aattcaaatg cacaggtgaa ggagaaggca
      241 gaccgtacga gggaactcaa accatgagga tcaaagtcat cgagggagga cccctgccat
      301 ttgcctttga cattcttgcc acgtcgttca tgtatggcag ccgtactttt atcaagtacc
      361 cggccgacat ccctgatttc tttaaacagt cctttcctga gggttttact tgggaaagag
      421 ttacgagata cgaagatggt ggagtcgtca ccgtcacgca ggacaccagc cttgaggatg
      481 gcgagctcgt ctacaacgtc aaggtcagag gggtaaactt tccctccaat ggtcccgtga
      541 tgcagaagaa gaccaagggt tgggagccta atacagagat gatgtatcca gcagatggtg
      601 gtctgagagg atacactgac atcgcactga aagttgatgg tggtggccat ctgcactgca
      661 acttcgtgac aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg cccggtgtcc
      721 atgccgttga tcaccgcctg gaaaggatcg aggagagtga caatgaaacc tacgtagtgc
      781 aacgcgaagt ggcagttgcc aaatacagca accttggtgg tggcatggac gagctgtaca
      841 agtcagctgc tttaatgaga tatgcgagac gcctatgatc gcatgatatt tgctttcaat
      901 tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta ccgccggttt
      961 cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat aatattctcc
     1021 gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg aaaacaatat
     1081 attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa caaacaattg
     1141 cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa acctaaaaga
     1201 ctgattacat aaatcttatt caaatttcaa aagtgcccca ggggctagta tctacgacac
     1261 accgagcggc gaactaataa cgctcactga agggaactcc ggttccccgc cggcgcgcat
     1321 gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt acgggcacca
     1381 ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga attatgcagc
     1441 atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac agtgacgaca
     1501 aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca gcaggacctg
     1561 caggcatgca agctagctta ctagtgatgc atattctata gtgtcaccta aatgtattct
     1621 agactcgttc gctgccacct aagaatactc tacggtcaca tactagggat aacagggtaa
     1681 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     1741 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     1801 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     1861 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     1921 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     1981 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     2041 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2101 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     2161 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2221 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     2281 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     2341 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     2401 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     2461 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     2521 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     2581 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     2641 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     2701 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     2761 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2821 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2881 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2941 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3001 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3061 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3121 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3181 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3241 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3301 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3361 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3421 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3481 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3541 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     3601 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3661 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
     3721 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
     3781 gcccgccacc ccttcgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     3841 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcatccga
     3901 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     3961 ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccc
//
