LOCUS       pGGIB-U4-pZmPLTP-ZmBBM-tnos-U5        4941 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...8e-aruiqnr
VERSION     urn.local...8e-aruiqnr
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_difference 1..18
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     misc_feature    19..58
                     /created_by="lapau"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:67-cctbnub"">U3</a>"
                     /label="U3"
                     /note="Geneious type: Golden Gibson Cloning site"
     promoter        71..1381
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmAXIG"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:75-c53yydv"">pZmAXIG</a>"
                     /label="pZmAXIG"
     CDS             1386..2294
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ZmWUS2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:6u-c53xntm"">ZmWUS2</a>"
                     /label="ZmWUS2"
     terminator      2299..2538
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="35ST"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1d-c4nnd54"">35ST</a>"
                     /label="35ST"
     misc_feature    2549..2588
                     /created_by="lapau"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:69-cctbnuc"">U4</a>"
                     /label="U4"
                     /note="Geneious type: Golden Gibson Cloning site"
     misc_difference complement(2589..2606)
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:laurenspauwels:2b-cg8gzu6"">I-SceI
                     site</a>"
                     /label="I-SceI site"
     primer          complement(2634..2654)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2715..2765
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2766..3575
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2o-c4nnd56"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3722..4310
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4910..4930
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 attaccctgt tatccctagc actgaaggtc ctcaatcgca ctggaaacat caaggtcgct
       61 cgagatacct aggcgaccca tcgctgcttt gtctacatca tgttcttcat catcctcccc
      121 aggcgacgcg tgctgctgtt cttattcaga ctaccgttcg agtgactgca tggcgtacat
      181 ctttctgcat cgactttgta cggctacatc gaacatatac acgagatgtc tcgtgtgaat
      241 agagtcacta atgccttaag catcggttac tccgtagggt acattctgtt cttcttattt
      301 gtgcatattt ttattgttgt ttactgatta tacgagtagt tatacataca tgcacataca
      361 tatcatcaca tatatcacaa tatttttcta aattaaatta aaactaaaaa tgactaaatt
      421 tctaacacca acgacattgt aatgttttct ccaacaactt tacctattct acattgttct
      481 atttcgaatt tcactctata aacaacatag tctacaatgg aaaacagtgc tttgtacgac
      541 tatatacgcg atgtgtggct acaacataag acaatatagt cgtttgaaga ttgaacctat
      601 atatcggtac ggttaatccg tctatgtacg tgggcatgac gaacacccgt gataacgaag
      661 gattaacgtg cacaatcata aatccaaagt aggagcggtg catgatgaga atcgctctca
      721 gtactcgaca taatgaacct tacgaggtac aacaggcagg caggcaggga ccaggggccg
      781 cctttatttc aggctcgctg gccccacggg cgtgctgcgt gcacgaaggg cactacccca
      841 acctctcacc gaaaaccgcg ctggatcggc aaatcaaacg aggtggtgcc ccgtgcccac
      901 tctccacgtc cacggcacca tccctctgca gccgctcacc agccatgccg tgtcgcggaa
      961 cggcacaacc acccccaacc cactcacgaa accccgtccc ggccgtgccc gtgtcggtcc
     1021 gcgctcggca acgaggcggc ccgcgctgct gagtcccctg gacacccgac accctgtcgg
     1081 ccctttgttt attcatcccg aaatctcatc tgcccccacg gccgactgcg ctgcgccgcc
     1141 cggatatata tacccatcgt tatcgatcga tcgatcgcgt cactcacggg tagctcatgg
     1201 tcgagcgtag catgcaggaa cttatttgcc gtgcgctccc agctctccgc tcgcgtgcct
     1261 tccagtctgt ctcacactag ctgctgtggg acgatcgaag tgggtgtgtc agctagctag
     1321 ctgcgccgtg accacgcaca tgaccgcagt gcgcgcgggg ctgatcaagg gaaagtgatc
     1381 cggctatggc ggccaatgcg ggcggcggtg gagcgggagg aggcagcggc agcggcagcg
     1441 tggctgcgcc ggcggtgtgc cgccccagcg gctcgcggtg gacgccgacg ccggagcaga
     1501 tcaggatgct gaaggagctc tactacggct gcggcatccg gtcgcccagc tcggagcaga
     1561 tccagcgcat caccgccatg ctgcggcagc acggcaagat cgagggcaag aacgtcttct
     1621 actggttcca gaaccacaag gcccgcgagc gccagaagcg ccgcctcacc agcctcgacg
     1681 tcaacgtgcc cgccgccggc gcggccgacg ccaccaccag ccaactcggc gtcctctcgc
     1741 tgtcgtcgcc gccgccttca ggcgcggcgc ctccctcgcc caccctcggc ttctacgccg
     1801 ccggcaatgg cggcggatcg gctgtgctgc tggacacgag ttccgactgg ggcagcagcg
     1861 gcgctgccat ggccaccgag acatgcttcc tgcaggacta catgggcgtg acggacacgg
     1921 gcagctcgtc gcagtggcca cgcttctcgt cgtcggacac gataatggcg gcggccgcgg
     1981 cgcgggcggc gacgacgcgg gcgcccgaga cgctccctct cttcccgacc tgcggcgacg
     2041 acggcggcag cggtagcagc agctacttgc cgttctgggg tgccgcgtcc acaactgccg
     2101 gcgccacttc ttccgttgcg atccagcagc aacaccagct gcaggagcag tacagctttt
     2161 acagcaacag caacagcacc cagctggccg gcaccggcaa ccaagacgta tcggcaacag
     2221 cagcagcagc cgccgccctg gagctgagcc tcagctcatg gtgctcccct taccctgctg
     2281 cagggagtat gtgatcagga taactagctg agcggccatg ctagagtccg caaaaatcac
     2341 cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt gtgagtagtt
     2401 cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc atataagaaa
     2461 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     2521 accaaaatcc agtgacctgt attctagact gacctcctgc cagcaatagt aagacaacac
     2581 gcaaagtcta gggataacag ggtaatgata tcccctatag tgagtcgtat tacatggtca
     2641 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     2701 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     2761 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
     2821 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     2881 atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     2941 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     3001 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     3061 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     3121 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     3181 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     3241 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     3301 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     3361 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     3421 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     3481 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     3541 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
     3601 actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta
     3661 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     3721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     3781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     3841 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     3901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     3961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     4201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     4261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     4321 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     4381 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     4441 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     4501 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     4561 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg
     4621 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
     4681 cctggcagtt tatggcgggc gtcctgcccg ccaccccttc gggccgttgc ttcacaacgt
     4741 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     4801 taaaacgaaa ggcccagtca tccgactgag cctttcgttt tatttgatgc ctggcagttc
     4861 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     4921 gccagtctta agctcgggcc c
//
