LOCUS       pGG-C-LoxP-ZmNLS-mScarlet-D        3206 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     Start           14..16
                     /created_by="lapau"
                     /modified_by="mamee"
                     /label="START"
     motif           17..50
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="loxP"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:8a-c541szf"">loxP</a>"
                     /label="LoxP"
     NLS             53..142
                     /created_by="lapau"
                     /modified_by="mamee"
                     /label="O2 NLS"
     CDS             143..841
                     /vntifkey=4
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mScarlet"
                     /Transferred_Similarity="99.86%"
                     /Adjusted_Transferred_Similarity="99.43%"
                     /Primary_Match="99.86%; Adjusted-Interval-0:0/-3
                     (adjustedto 99.43%; Adjusted-Interval-0:0/0); mScarlet-L
                     (<ahref=""urn:local:lapau:r-e4ew2m2"">mScarlet</a>)"
                     /Alternative_Match="89.97%; Adjusted-Interval-0:0/-3
                     (adjusted to 89.59%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:thjac:3a-c4nnd56"">mCherry</a>"
                     /Alternative_Match="67.24%; Adjusted-Interval-0:3/-3
                     (adjusted to 66.67%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:warddecaestecker:1k-c4o0i72"">TagRFP</a>"
                     /Alternative_Match="63.43%; Adjusted-Interval-0:0/-3
                     (adjusted to 63.17%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:thjac:3g-c4nnd56"">mRuby3</a>"
                     /modified_by="mamee"
                     /label="mScarlet-L*"
     GG              complement(842..852)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /Alternative_Match="90.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2l-c4nnd55"">E</a>"
                     /label="D"
     rep_origin      complement(1083..1671)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     misc_feature    complement(1842..2702)
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="99.42%"
                     /Primary_Match="99.42%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(2703..2807)
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
ORIGIN      
        1 ggtctcaggc tccatgataa cttcgtatag catacattat acgaagttat cccctaccga
       61 ggaaagagtg aggaaaagaa aggaatccaa tagagaatca gccagacgct cgagatacag
      121 gaaagccgct cacctgaaag aactggtgag caagggcgag gcagtgatca aggagttcat
      181 gcggttcaag gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga
      241 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
      301 ccccctgccc ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt
      361 caccaagcac cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa
      421 gtgggagcgc gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc
      481 cctggaggac ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga
      541 cggccccgta atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc
      601 cgaggacggc gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg
      661 ctacctggcg gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc
      721 ctacaacgtc gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga
      781 acagtacgaa cgctccgagg gccgccactc caccggcggc atggacgagc tgtacaagtg
      841 atcagtgaga ccgatatcct gggcctcatg ggccttccgc tcactgcccg ctttccagtc
      901 gggaaacctg tcgtgccagc tgcattaaca tggtcatagc tgtttccttg cgtattgggc
      961 gctctccgct tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa agcctggggt
     1021 gcctaatgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1081 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     1141 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1201 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1261 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1321 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     1381 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1441 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1501 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1561 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1621 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1681 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     1741 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     1801 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     1861 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1921 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1981 cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2041 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2101 gaagctagag taagtagttc gccagttaat agtttgcgca atgttgttgc cattgctacg
     2161 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2221 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2281 ccgatcgttg tcagaagtaa gttggccgca gtgttatcgc tcatggttat ggcagcactg
     2341 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2401 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2461 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     2521 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     2581 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     2641 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     2701 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     2761 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     2821 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     2881 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaaa
     2941 gaatagaccg agatagggtt gagtggccgc tacagggcgc tcccattcgc cattcaggct
     3001 gcgcaactgt tgggaagggc gtttcggtgc gggcctcttc gctattacgc cagctggcga
     3061 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     3121 gttgtaaaac gacggccagt gagcgcgacg taatacgact cactataggg cgaattggcg
     3181 gaaggccgtc aaggccgcat gatatc
//
