LOCUS       pGG-C-ZmWUS2-D          3597 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...4s2-95xpsjt
VERSION     urn.local...4s2-95xpsjt
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             14..922
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ZmWUS2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:6u-c53xntm"">ZmWUS2</a>"
                     /modified_by="mamee"
                     /label="ZmWUS2*"
     GG              complement(923..933)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:298-ciz70tq"">D</a>"
                     /label="D"
     primer          complement(953..973)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1453..1557
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1558..2418
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2589..3177
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3541..3562
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggcgg ccaatgcggg cggcggtgga gcgggaggag gcagcggcag
       61 cggcagcgtg gctgcgccgg cggtgtgccg ccccagcggc tcgcggtgga cgccgacgcc
      121 ggagcagatc aggatgctga aggagctcta ctacggctgc ggcatccggt cgcccagctc
      181 ggagcagatc cagcgcatca ccgccatgct gcggcagcac ggcaagatcg agggcaagaa
      241 cgtcttctac tggttccaga accacaaggc ccgcgagcgc cagaagcgcc gcctcaccag
      301 cctcgacgtc aacgtgcccg ccgccggcgc ggccgacgcc accaccagcc aactcggcgt
      361 cctctcgctg tcgtcgccgc cgccttcagg cgcggcgcct ccctcgccca ccctcggctt
      421 ctacgccgcc ggcaatggcg gcggatcggc tgtgctgctg gacacgagtt ccgactgggg
      481 cagcagcggc gctgccatgg ccaccgagac atgcttcctg caggactaca tgggcgtgac
      541 ggacacgggc agctcgtcgc agtggccacg cttctcgtcg tcggacacga taatggcggc
      601 ggccgcggcg cgggcggcga cgacgcgggc gcccgagacg ctccctctct tcccgacctg
      661 cggcgacgac ggcggcagcg gtagcagcag ctacttgccg ttctggggtg ccgcgtccac
      721 aactgccggc gccacttctt ccgttgcgat ccagcagcaa caccagctgc aggagcagta
      781 cagcttttac agcaacagca acagcaccca gctggccggc accggcaacc aagacgtatc
      841 ggcaacagca gcagcagccg ccgccctgga gctgagcctc agctcatggt gctcccctta
      901 ccctgctgca gggagtatgt gatcagtgag accgaattct cgccctatag tgagtcgtat
      961 tacctggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1021 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1081 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     1141 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     1201 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     1261 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     1321 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     1381 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     1441 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1501 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1561 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1621 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1681 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1741 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1801 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1861 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1921 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1981 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2041 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2101 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2161 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2221 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tggctctcga
     2281 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2341 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2401 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2461 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2521 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2581 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2641 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2701 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     2761 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2821 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2881 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     2941 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3001 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3061 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3121 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3181 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3241 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3301 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3361 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     3421 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     3481 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     3541 gtgagcggat aacaatttca cacgagctat ttaggtgaca ctatagaagt gaagctt
//
