LOCUS       pGG-C-ZmBBM-D           4821 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...4p2-95xpshg
VERSION     urn.local...4p2-95xpshg
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:88-dgycryy"">C</a>"
                     /label="C"
     CDS             14..2146
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ZmODP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:6p-c53xikd"">ZmODP2</a>"
                     /modified_by="mamee"
                     /label="ZmODP2*"
     GG              complement(2147..2157)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:81-dgycryx"">D</a>"
                     /Alternative_Match="90.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8b-dgycryy"">E</a>"
                     /label="D"
     misc_feature    2150^2151
                     /Original_Bases="TCAG"
                     /label="TCAG"
                     /note="Geneious type: Editing History Deletion"
     primer          complement(2177..2197)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        2677..2781
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2782..3642
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3813..4401
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4765..4786
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggcca ctgtgaacaa ctggctcgct ttctccctct ccccgcagga
       61 gctgccgccc tcccagacga cggactccac actcatctcg gccgccaccg ccgaccatgt
      121 ctccggcgat gtctgcttca acatccccca agattggagc atgaggggat cagagctttc
      181 ggcgctcgtc gcggagccga agctggagga cttcctcggc ggcatctcct tctccgagca
      241 gcatcacaag gccaactgca acatgatacc cagcactagc agcacagttt gctacgcgag
      301 ctcaggtgct agcaccggct accatcacca gctgtaccac cagcccacca gctcagcgct
      361 ccacttcgcg gactccgtaa tggtggcctc ctcggccggt gtccacgacg gcggtgccat
      421 gctcagcgcg gccgccgcta acggtgtcgc tggcgctgcc agtgccaacg gcggcggcat
      481 cgggctgtcc atgattaaga actggctgcg gagccaaccg gcgcccatgc agccgagggt
      541 ggcggcggct gagggcgcgc aggggctctc tttgtccatg aacatggcgg ggacgaccca
      601 aggcgctgct ggcatgccac ttctcgctgg agagcgcgca cgggcgcccg agagtgtatc
      661 gacgtcagca cagggtggag ccgtcgtcgt cacggcgccg aaggaggata gcggtggcag
      721 cggtgttgcc ggcgctctag tagccgtgag cacggacacg ggtggcagcg gcggcgcgtc
      781 ggctgacaac acggcaagga agacggtgga cacgttcggg cagcgcacgt cgatttaccg
      841 tggcgtgaca aggcatagat ggactgggag atatgaggca catctttggg ataacagttg
      901 cagaagggaa gggcaaactc gtaagggtcg tcaagtctat ttaggtggct atgataaaga
      961 ggagaaagct gctagggctt atgatcttgc tgctctgaag tactggggtg ccacaacaac
     1021 aacaaatttt ccagtgagta actacgaaaa ggagctcgag gacatgaagc acatgacaag
     1081 gcaggagttt gtagcgtctc tgagaaggaa gagcagtggt ttctccagag gtgcatccat
     1141 ttacagggga gtgactaggc atcaccaaca tggaagatgg caagcacgga ttggacgagt
     1201 tgcagggaac aaggatcttt acttgggcac cttcagcacc caggaggagg cagcggaggc
     1261 gtacgacatc gcggcgatca agttccgcgg cctcaacgcc gtcaccaact tcgacatgag
     1321 ccgctacgac gtgaagagca tcctggacag cagcgccctc cccatcggca gcgccgccaa
     1381 gcgcctcaag gaggccgagg ccgcagcgtc cgcgcagcac caccacgccg gcgtggtgag
     1441 ctacgacgtc ggccgcatcg cctcgcagct cggcgacggc ggagccctgg cggcggcgta
     1501 cggcgcgcac taccacggcg ccgcctggcc gaccatcgcg ttccagccgg gcgccgccag
     1561 cacaggcctg taccacccgt acgcgcagca gccaatgcgc ggcggcgggt ggtgcaagca
     1621 ggagcaggac cacgcggtga tcgcggccgc gcacagcctg caggacctcc accacctgaa
     1681 cctgggcgcg gccggcgcgc acgacttttt ctcggcaggg cagcaggccg ccgccgctgc
     1741 gatgcacggc ctgggtagca tcgacagtgc gtcgctcgag cacagcaccg gctccaactc
     1801 cgtcgtctac aacggcgggg tcggcgacag caacggcgcc agcgccgtcg gcggcagtgg
     1861 cggtggctac atgatgccga tgagcgctgc cggagcaacc actacatcgg caatggtgag
     1921 ccacgagcag gtgcatgcac gggcctacga cgaagccaag caggctgctc agatggggta
     1981 cgagagctac ctggtgaacg cggagaacaa tggtggcgga aggatgtctg catgggggac
     2041 tgtcgtgtct gcagccgcgg cggcagcagc aagcagcaac gacaacatgg ccgccgacgt
     2101 cggccatggc ggcgcgcagc tcttcagtgt ctggaacgac acttaatcag tgagaccgaa
     2161 ttctcgccct atagtgagtc gtattacctg gccgtcgttt tacaacgtcg tgactgggaa
     2221 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2281 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2341 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     2401 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     2461 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     2521 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     2581 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     2641 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2701 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2761 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2821 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2881 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2941 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3001 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     3061 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3121 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3181 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3241 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3301 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3361 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3421 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3481 ggagccggtg agcgtggctc tcgaggtatc attgcagcac tggggccaga tggtaagccc
     3541 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3601 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3661 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3721 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3781 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3841 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3901 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3961 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4021 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4081 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4141 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4201 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4261 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4321 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4381 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4441 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4501 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4561 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4621 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4681 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     4741 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacgag ctatttaggt
     4801 gacactatag aagtgaagct t
//
