LOCUS       pGG-A-pZmGLB1-C         4122 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   p00012
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..1447
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmGLB1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:72-c53xug4"">pZmGLB1</a>"
                     /label="pZmGLB1"
     GG              complement(1448..1458)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(1478..1498)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1978..2082
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    2083..2943
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3114..3702
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4066..4087
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tgcttgccga gtgccatcct tggacactcg ataaagtata ttttattttt
       61 tttattttgc caaccaaact ttttgtggta tgttcctaca ctatgtagat ctacatgtac
      121 cattttggca caattacata tttacaaaaa tgttttctat aaatattaga tttagttcgt
      181 ttatttgaat ttcttcggaa aattcacatt taaactgcaa gtcactcgaa acatggaaaa
      241 ccgtgcatgc aaaataaatg atatgcatgt tatctagcac aagttacgac cgatttcaga
      301 agcagaccag aatcttcaag caccatgctc actaaacatg accgtgaact tgttatctag
      361 ttgtttaaaa attgtataaa acacaaataa agtcagaaat taatgaaact tgtccacatg
      421 tcatgatatc atatatagag gttgtgataa aaatttgata atgtttcggt aaagttgtga
      481 cgtactatgt gtagaaacct aagtgaccta cacataaaat catagagttt caatgtagtt
      541 cactcgacaa agactttgtc aagtgtccga taaaaagtac tcgacaaaga agccgttgtc
      601 gatgtactgt tcgtcgagat ctctttgtcg agtgtcacac taggcaaagt ctttacggag
      661 tgtttttcag gctttgacac tcggcaaagc gctcgattcc agtagtgaca gtaatttgca
      721 tcaaaaatag ctgagagatt taggccccgt ttcaatctca cgggataaag tttagcttcc
      781 tgctaaactt tagctatatg aattgaagtg ctaaagttta gtttcaatta ccaccattag
      841 ctctcctgtt tagattacaa atggctaaaa gtagctaaaa aatagctgct aaagtttatc
      901 tcgcgagatt gaaacagggc cttaaaatga gtcaactaat agaccaacta attattagct
      961 attagtcgtt agcttcttta atctaagcta aaaccaacta atagcttatt tgttgaatta
     1021 caattagctc aacggaattc tctgtttttt ctataaaaaa agggaaactg cccctcattt
     1081 acagcaaatt gtccgctgcc tgtcgtccag atacaatgaa cgtacctagt aggaactctt
     1141 ttacacgctc ggtcgctcgc cgcggatcgg agtcccagga acacgacacc actgtgtaac
     1201 acgacaaagt ctgctcagag gcggccacac cctggcgtgc accgagccgg agcccggata
     1261 agcacggtaa ggagagtacg gcgggacgtg gcgacccgtg tgtctgctgc cacgcagcct
     1321 tcctccacgt agccgcgcgg ccgcgccacg taccagggcc cggcgctggt ataaatgcgc
     1381 gctacctccg ctttagttct gcatacagtc aacctaacac acccgagcat atcacagtga
     1441 cagacacggc ttgagaccga attctcgccc tatagtgagt cgtattacct ggccgtcgtt
     1501 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     1561 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     1621 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     1681 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     1741 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     1801 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     1861 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     1921 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     1981 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2041 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     2101 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2161 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2221 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2281 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2341 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2401 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2461 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2521 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2581 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2641 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2701 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2761 tggtttattg ctgataaatc tggagccggt gagcgtggct ctcgaggtat cattgcagca
     2821 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2881 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2941 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     3001 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3061 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3121 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3181 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3241 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3301 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3361 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3421 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3481 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3541 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3601 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3661 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3721 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3781 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3841 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3901 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3961 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     4021 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4081 tttcacacga gctatttagg tgacactata gaagtgaagc tt
//
