LOCUS       pGG-A-pZmAXIG1-C        4002 bp    DNA     circular     23-FEB-2023
DEFINITION  .
ACCESSION   p00012
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8a-dgycryy"">A</a>"
                     /label="A"
     promoter        12..1322
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmAXIG"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:75-c53yydv"">pZmAXIG</a>"
                     /label="pZmAXIG"
     GG              complement(1323..1333)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:80-dgycryx"">C</a>"
                     /label="C"
     misc_feature    1327
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     primer          complement(1358..1378)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1858..1962
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1963..2823
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2994..3582
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3946..3967
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc taggcgaccc atcgctgctt tgtctacatc atgttcttca tcatcctccc
       61 caggcgacgc gtgctgctgt tcttattcag actaccgttc gagtgactgc atggcgtaca
      121 tctttctgca tcgactttgt acggctacat cgaacatata cacgagatgt ctcgtgtgaa
      181 tagagtcact aatgccttaa gcatcggtta ctccgtaggg tacattctgt tcttcttatt
      241 tgtgcatatt tttattgttg tttactgatt atacgagtag ttatacatac atgcacatac
      301 atatcatcac atatatcaca atatttttct aaattaaatt aaaactaaaa atgactaaat
      361 ttctaacacc aacgacattg taatgttttc tccaacaact ttacctattc tacattgttc
      421 tatttcgaat ttcactctat aaacaacata gtctacaatg gaaaacagtg ctttgtacga
      481 ctatatacgc gatgtgtggc tacaacataa gacaatatag tcgtttgaag attgaaccta
      541 tatatcggta cggttaatcc gtctatgtac gtgggcatga cgaacacccg tgataacgaa
      601 ggattaacgt gcacaatcat aaatccaaag taggagcggt gcatgatgag aatcgctctc
      661 agtactcgac ataatgaacc ttacgaggta caacaggcag gcaggcaggg accaggggcc
      721 gcctttattt caggctcgct ggccccacgg gcgtgctgcg tgcacgaagg gcactacccc
      781 aacctctcac cgaaaaccgc gctggatcgg caaatcaaac gaggtggtgc cccgtgccca
      841 ctctccacgt ccacggcacc atccctctgc agccgctcac cagccatgcc gtgtcgcgga
      901 acggcacaac cacccccaac ccactcacga aaccccgtcc cggccgtgcc cgtgtcggtc
      961 cgcgctcggc aacgaggcgg cccgcgctgc tgagtcccct ggacacccga caccctgtcg
     1021 gccctttgtt tattcatccc gaaatctcat ctgcccccac ggccgactgc gctgcgccgc
     1081 ccggatatat atacccatcg ttatcgatcg atcgatcgcg tcactcacgg gtagctcatg
     1141 gtcgagcgta gcatgcagga acttatttgc cgtgcgctcc cagctctccg ctcgcgtgcc
     1201 ttccagtctg tctcacacta gctgctgtgg gacgatcgaa gtgggtgtgt cagctagcta
     1261 gctgcgccgt gaccacgcac atgaccgcag tgcgcgcggg gctgatcaag ggaaagtgat
     1321 ccggcttgag accgatatca attctcgccc tatagtgagt cgtattacct ggccgtcgtt
     1381 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     1441 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     1501 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     1561 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     1621 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     1681 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     1741 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     1801 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     1861 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1921 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1981 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     2041 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     2101 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     2161 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     2221 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     2281 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     2341 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     2401 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     2461 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     2521 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     2581 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     2641 tggtttattg ctgataaatc tggagccggt gagcgtggct ctcgaggtat cattgcagca
     2701 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     2761 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     2821 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     2881 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     2941 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3001 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3061 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3121 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3181 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3241 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3301 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3361 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3421 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3481 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3541 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3601 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3661 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3721 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3781 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3841 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3901 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3961 tttcacacga gctatttagg tgacactata gaagtggata tc
//
