LOCUS       pEN-L1-pZmAXIG1-ZmWUS2-35ST-L2        5058 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...jt-b5ybzix
VERSION     urn.local...jt-b5ybzix
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     attL1           1..100
                     /Color="#00cccc"
                     /created_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL1"
                     /Transferred_Similarity="99.00%"
                     /Primary_Match="99.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:li-eztyqs1"">attL1</a>"
                     /label="attL1"
     promoter        107..1417
                     /created_by="lapau"
                     /modified_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmAXIG"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:75-c53yydv"">pZmAXIG</a>"
                     /label="pZmAXIG"
     CDS             1424..2332
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ZmWUS2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:6u-c53xntm"">ZmWUS2</a>"
                     /label="ZmWUS2"
     terminator      2337..2576
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="35ST"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1d-c4nnd54"">35ST</a>"
                     /label="35ST"
     attL2           complement(2610..2709)
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL2"
                     /Transferred_Similarity="98.00%"
                     /Primary_Match="98.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:ll-eztyzh8"">attL2</a>"
                     /label="attL2"
     primer          complement(2752..2772)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2833..2883
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2884..3693
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2o-c4nnd56"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3840..4428
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5027..5047
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct taacctaggc gacccatcgc
      121 tgctttgtct acatcatgtt cttcatcatc ctccccaggc gacgcgtgct gctgttctta
      181 ttcagactac cgttcgagtg actgcatggc gtacatcttt ctgcatcgac tttgtacggc
      241 tacatcgaac atatacacga gatgtctcgt gtgaatagag tcactaatgc cttaagcatc
      301 ggttactccg tagggtacat tctgttcttc ttatttgtgc atatttttat tgttgtttac
      361 tgattatacg agtagttata catacatgca catacatatc atcacatata tcacaatatt
      421 tttctaaatt aaattaaaac taaaaatgac taaatttcta acaccaacga cattgtaatg
      481 ttttctccaa caactttacc tattctacat tgttctattt cgaatttcac tctataaaca
      541 acatagtcta caatggaaaa cagtgctttg tacgactata tacgcgatgt gtggctacaa
      601 cataagacaa tatagtcgtt tgaagattga acctatatat cggtacggtt aatccgtcta
      661 tgtacgtggg catgacgaac acccgtgata acgaaggatt aacgtgcaca atcataaatc
      721 caaagtagga gcggtgcatg atgagaatcg ctctcagtac tcgacataat gaaccttacg
      781 aggtacaaca ggcaggcagg cagggaccag gggccgcctt tatttcaggc tcgctggccc
      841 cacgggcgtg ctgcgtgcac gaagggcact accccaacct ctcaccgaaa accgcgctgg
      901 atcggcaaat caaacgaggt ggtgccccgt gcccactctc cacgtccacg gcaccatccc
      961 tctgcagccg ctcaccagcc atgccgtgtc gcggaacggc acaaccaccc ccaacccact
     1021 cacgaaaccc cgtcccggcc gtgcccgtgt cggtccgcgc tcggcaacga ggcggcccgc
     1081 gctgctgagt cccctggaca cccgacaccc tgtcggccct ttgtttattc atcccgaaat
     1141 ctcatctgcc cccacggccg actgcgctgc gccgcccgga tatatatacc catcgttatc
     1201 gatcgatcga tcgcgtcact cacgggtagc tcatggtcga gcgtagcatg caggaactta
     1261 tttgccgtgc gctcccagct ctccgctcgc gtgccttcca gtctgtctca cactagctgc
     1321 tgtgggacga tcgaagtggg tgtgtcagct agctagctgc gccgtgacca cgcacatgac
     1381 cgcagtgcgc gcggggctga tcaagggaaa gtgatccggc tccatggcgg ccaatgcggg
     1441 cggcggtgga gcgggaggag gcagcggcag cggcagcgtg gctgcgccgg cggtgtgccg
     1501 ccccagcggc tcgcggtgga cgccgacgcc ggagcagatc aggatgctga aggagctcta
     1561 ctacggctgc ggcatccggt cgcccagctc ggagcagatc cagcgcatca ccgccatgct
     1621 gcggcagcac ggcaagatcg agggcaagaa cgtcttctac tggttccaga accacaaggc
     1681 ccgcgagcgc cagaagcgcc gcctcaccag cctcgacgtc aacgtgcccg ccgccggcgc
     1741 ggccgacgcc accaccagcc aactcggcgt cctctcgctg tcgtcgccgc cgccttcagg
     1801 cgcggcgcct ccctcgccca ccctcggctt ctacgccgcc ggcaatggcg gcggatcggc
     1861 tgtgctgctg gacacgagtt ccgactgggg cagcagcggc gctgccatgg ccaccgagac
     1921 atgcttcctg caggactaca tgggcgtgac ggacacgggc agctcgtcgc agtggccacg
     1981 cttctcgtcg tcggacacga taatggcggc ggccgcggcg cgggcggcga cgacgcgggc
     2041 gcccgagacg ctccctctct tcccgacctg cggcgacgac ggcggcagcg gtagcagcag
     2101 ctacttgccg ttctggggtg ccgcgtccac aactgccggc gccacttctt ccgttgcgat
     2161 ccagcagcaa caccagctgc aggagcagta cagcttttac agcaacagca acagcaccca
     2221 gctggccggc accggcaacc aagacgtatc ggcaacagca gcagcagccg ccgccctgga
     2281 gctgagcctc agctcatggt gctcccctta ccctgctgca gggagtatgt gatcaggata
     2341 actagctgag cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat
     2401 ctctctctat ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt
     2461 cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     2521 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctgtat
     2581 tacctacagg tcactaatac catctaagta gtcagctttc ttgtacaaag ttggcattat
     2641 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2701 cattatttgt gatgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     2761 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2821 aaaataatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2881 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2941 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     3001 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     3061 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     3121 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     3181 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     3241 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     3301 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     3361 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     3421 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     3481 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     3541 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     3601 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     3661 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     3721 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     3781 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3841 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3901 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3961 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4021 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4081 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4141 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4201 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4261 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4321 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4381 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4441 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4501 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     4561 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     4621 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     4681 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     4741 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     4801 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     4861 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
     4921 aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
     4981 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
     5041 agtcttaagc tcgggccc
//
