LOCUS       pEN-L4-pZmPLTP-ZmBBM-G7T-R1        6131 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...lw-eqt4mz7
VERSION     urn.local...lw-eqt4mz7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     attL4           1..97
                     /modified_by="mamee"
                     /label="attL4"
     promoter        104..1165
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmLTP"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:79-c53z84x"">pZmLTP</a>"
                     /label="pZmLTP"
     CDS             1172..3304
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ZmODP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:6p-c53xikd"">ZmODP2</a>"
                     /label="ZmODP2"
     terminator      3309..3580
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G7T"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1a-c4nnd53"">G7T</a>"
                     /label="G7T"
     attR1           3586..3709
                     /modified_by="mamee"
                     /label="attR1"
     primer          complement(3802..3822)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        3883..3933
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    3934..4743
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2o-c4nnd56"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4890..5478
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.49%"
                     /Primary_Match="99.49%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6077..6097
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtataga aaagttgcta cctccctagc tccctgcggc
      121 tgttacgcgg tcccccatca atcttctgtt cttgcggttg tagcctgtgt aacagtgcta
      181 gagtatgtat gataaatagg ttttaagtct gcttacatga cattttttat tgtggaagag
      241 acatataaaa attagagaga gtggttctca tgcaacggcg gacggcccgg tgctaaaaga
      301 gcttcaagac aaaataatga aacaggaaga gagtagattt atctaagagc caactttatt
      361 atatgaatgt gtttattgtt ggctttagat gatatggtaa ggagttagag ctaataatag
      421 ataggctcta ttattattat tattaattaa actcgctcta aggaggaaag tgggaggaag
      481 ggacgaggac gatgactact ggaagcatcg tgcatggatg atggatgtgg tgtctcttaa
      541 tgtaggtggc cggaggatgt acgtgttaat tgcgcgataa gcactcagat ccaaccgcaa
      601 actacctcca cactgacaca ctgatagaga gaaagagaga gctccgacga ctgccgccgc
      661 agatgagcca cgtacgtata cgacgtctgc cggccggctc aggctgccgc catcaccctg
      721 ctcgaaagtc gcgttaggcg gcgccagcta cataggagta tctagtctag ccagttagta
      781 tactactact gcgctgatga tgaattaact ctgcatagat actgtacttg cctccctcca
      841 acacccaacc acctcctgct cggctcttaa taacttggac acggatcgat gccatccaag
      901 gaagaacacg acgacgacga cggaacatcc accatgcaag cttgcatcca tacgccgata
      961 cgcgtgcatc catccatcca ccattatttc cattttccac cgatcacacg tacacaggcc
     1021 tatttaagga gcgacatccc actgcaactc tcctcaccac tcatcaccag ctagctctag
     1081 caaagcactt gccatctacc gaccgccgca ttccaaacag cccgacgagc tagcagagcg
     1141 gcaggcacct ccctcctcaa ggaacggctc catggccact gtgaacaact ggctcgcttt
     1201 ctccctctcc ccgcaggagc tgccgccctc ccagacgacg gactccacac tcatctcggc
     1261 cgccaccgcc gaccatgtct ccggcgatgt ctgcttcaac atcccccaag attggagcat
     1321 gaggggatca gagctttcgg cgctcgtcgc ggagccgaag ctggaggact tcctcggcgg
     1381 catctccttc tccgagcagc atcacaaggc caactgcaac atgataccca gcactagcag
     1441 cacagtttgc tacgcgagct caggtgctag caccggctac catcaccagc tgtaccacca
     1501 gcccaccagc tcagcgctcc acttcgcgga ctccgtaatg gtggcctcct cggccggtgt
     1561 ccacgacggc ggtgccatgc tcagcgcggc cgccgctaac ggtgtcgctg gcgctgccag
     1621 tgccaacggc ggcggcatcg ggctgtccat gattaagaac tggctgcgga gccaaccggc
     1681 gcccatgcag ccgagggtgg cggcggctga gggcgcgcag gggctctctt tgtccatgaa
     1741 catggcgggg acgacccaag gcgctgctgg catgccactt ctcgctggag agcgcgcacg
     1801 ggcgcccgag agtgtatcga cgtcagcaca gggtggagcc gtcgtcgtca cggcgccgaa
     1861 ggaggatagc ggtggcagcg gtgttgccgg cgctctagta gccgtgagca cggacacggg
     1921 tggcagcggc ggcgcgtcgg ctgacaacac ggcaaggaag acggtggaca cgttcgggca
     1981 gcgcacgtcg atttaccgtg gcgtgacaag gcatagatgg actgggagat atgaggcaca
     2041 tctttgggat aacagttgca gaagggaagg gcaaactcgt aagggtcgtc aagtctattt
     2101 aggtggctat gataaagagg agaaagctgc tagggcttat gatcttgctg ctctgaagta
     2161 ctggggtgcc acaacaacaa caaattttcc agtgagtaac tacgaaaagg agctcgagga
     2221 catgaagcac atgacaaggc aggagtttgt agcgtctctg agaaggaaga gcagtggttt
     2281 ctccagaggt gcatccattt acaggggagt gactaggcat caccaacatg gaagatggca
     2341 agcacggatt ggacgagttg cagggaacaa ggatctttac ttgggcacct tcagcaccca
     2401 ggaggaggca gcggaggcgt acgacatcgc ggcgatcaag ttccgcggcc tcaacgccgt
     2461 caccaacttc gacatgagcc gctacgacgt gaagagcatc ctggacagca gcgccctccc
     2521 catcggcagc gccgccaagc gcctcaagga ggccgaggcc gcagcgtccg cgcagcacca
     2581 ccacgccggc gtggtgagct acgacgtcgg ccgcatcgcc tcgcagctcg gcgacggcgg
     2641 agccctggcg gcggcgtacg gcgcgcacta ccacggcgcc gcctggccga ccatcgcgtt
     2701 ccagccgggc gccgccagca caggcctgta ccacccgtac gcgcagcagc caatgcgcgg
     2761 cggcgggtgg tgcaagcagg agcaggacca cgcggtgatc gcggccgcgc acagcctgca
     2821 ggacctccac cacctgaacc tgggcgcggc cggcgcgcac gactttttct cggcagggca
     2881 gcaggccgcc gccgctgcga tgcacggcct gggtagcatc gacagtgcgt cgctcgagca
     2941 cagcaccggc tccaactccg tcgtctacaa cggcggggtc ggcgacagca acggcgccag
     3001 cgccgtcggc ggcagtggcg gtggctacat gatgccgatg agcgctgccg gagcaaccac
     3061 tacatcggca atggtgagcc acgagcaggt gcatgcacgg gcctacgacg aagccaagca
     3121 ggctgctcag atggggtacg agagctacct ggtgaacgcg gagaacaatg gtggcggaag
     3181 gatgtctgca tgggggactg tcgtgtctgc agccgcggcg gcagcagcaa gcagcaacga
     3241 caacatggcc gccgacgtcg gccatggcgg cgcgcagctc ttcagtgtct ggaacgacac
     3301 ttaatcagcc ccgatgagct aagctagcta tatcatcaat ttatgtatta cacataatat
     3361 cgcactcagt ctttcatcta cggcaatgta ccagctgata taatcagtta ttgaaatatt
     3421 tctgaattta aacttgcatc aataaattta tgtttttgct tggactataa tacctgactt
     3481 gttattttat caataaatat ttaaactata tttctttcaa gatgggaatt aacatctaca
     3541 aattgccttt tcttatcgac catgtactcg acggccgagt gtatgcaagt ttgtacaaaa
     3601 aagttgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
     3661 aaaaacagac tacataatac tgtaaaacac aacatatgca gtcactatga atcaactact
     3721 tagatggtat tagtgacctg tagaattcga gctctagagc tgcagggcgg ccgcgatatc
     3781 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     3841 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     3901 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     3961 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     4021 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     4081 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     4141 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     4201 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     4261 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     4321 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     4381 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     4441 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     4501 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     4561 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     4621 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     4681 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     4741 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     4801 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     4861 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4921 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4981 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     5041 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5101 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     5161 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     5221 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     5281 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5341 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5401 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5461 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5521 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5581 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5641 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     5701 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     5761 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     5821 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     5881 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     5941 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     6001 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     6061 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggcccgc gttaacgcta
     6121 ccatggagct c
//
