LOCUS       pEN-R2-pBdEF1a-GUS-35ST-L3        6745 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...3l8-95xprmj
VERSION     urn.local...3l8-95xprmj
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     attR2           complement(1..124)
                     /modified_by="mamee"
                     /label="attR2"
     promoter        131..2130
                     /modified_by="lapau"
                     /label="pBdEF1a.1"
     regulatory      2131..2133
                     /created_by="lapau"
                     /label="ATG"
     CDS             2173..3982
                     /vntifkey=4
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Gus-intron"
                     /Transferred_Similarity="99.78%"
                     /Primary_Match="99.78%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; <a
                     href=""urn:local:thjac:1l-cj7y2zc"">Gus-intron</a>"
                     /modified_by="lapau"
                     /label="GUS"
     terminator      3986..4225
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="35ST"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1d-c4nnd54"">35ST</a>"
                     /label="35ST"
     attL3           complement(4231..4327)
                     /modified_by="mamee"
                     /label="attL3"
     primer          complement(4384..4404)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        4465..4515
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    4516..5325
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2o-c4nnd56"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      5472..6060
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.49%"
                     /Primary_Match="99.49%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          6659..6679
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggctacct agatttgtga ttgttcagct acatgctcct tgaagcacct ttattctact
      181 ataaattagt tcgttggacg agactattag accacatgct agccatgctt gttcgtagtt
      241 gtcttcaccg ccattgcaaa aattgtcaat aaatatttag agtgggtggc atcagaaaaa
      301 catctctagt ggactctctt cctatcatag ctactcgggc tgtagataga acgagggcac
      361 aagagttggg tggcgtaggt ttactcgtga cctcaactct tttggctgtg tcttacgtct
      421 aagatgggtt tggcatgtga gaaacatagg tctaagcaat tcatgttagg gctgttgcat
      481 tgttgttgca tcaaccaaat gtccagatag cagttcatgc tacatctagt tgaaaaccct
      541 catcattagg cggaacatgt gttctttttt agcatagtca aagtcagatt gcggcactcg
      601 ctcatccacg gaaagaattt tccctgtgca ggcatctcga tcaaaagacg caaattaatt
      661 tttgaatagc gatataacaa tatctaatta acgtttcttg ttttctgcga aatgtctttc
      721 atcataaaat gagtcatctc gatgagccca agtgacatag cccaacaccc caccccacca
      781 ataaaagtga agaaaacatg ttgggaaaac tataccaagt aaaatacgag ttgttctaaa
      841 gaaaaagtaa agtacgagtt agatcgcacc ctgtcctgga gtgtggcttg atgatccaac
      901 tcctagcatt gtatccctgt ttttggatga tgtaactatt atttacaatg aataaagagg
      961 tgttttacta gtaaaaaaat cttgagggga ggagaaaata atggaggtct tttttcaaac
     1021 cgatggacta ttatttttag tgaaagagaa taatattatt ggaaaaatta ttctatccac
     1081 ttattttata ttggcagaat acaaagaatg gtggggtcca cgcggaactt gcggcccccg
     1141 aaacctatcg agggcgcggt acccaagcaa ggaacggagg aaacttgcgg ggcccgaaac
     1201 ctagtgataa aaggcatatc atccacacga tgaagatctg acggaccata tctcccacca
     1261 cggaaagcca tcagacgagg atcagacggc caggaaggaa ccctagcgcc cgccggtgcc
     1321 aatataaagc gccactctct ctcgtcttaa gccccagcct ctccattccc ctctccctct
     1381 cgccgccgcc gtctccttct cctactccct tcgaggtgtg ttgttcatcc gtcccgaatc
     1441 catccatccc ctcttcagat gtgttgttca tggctctaat agctctagat ctgcttgttt
     1501 gtgttgttta gctctagatc tactcgcgcg cgcttctctc tcgatctcct gtagaacaat
     1561 tttggttggt tttttgtgca tatccatggt aattttgtct gcaatatgga ggaggctttc
     1621 taagctccta cgtagcatcg atctttagaa ttccctcggt ttctgtttat ttcttcgcga
     1681 gggctctctg ttatctgtag gagtagctgt aagcgcggtt cgttacggat taatcgtcat
     1741 gcttagttga acctatcggt cgaaggattt gtgtgggttg tcgtgtagaa ttgacaccat
     1801 ctacttactg tactgatatg ccgatctgta ggatactctt cattactttt gtttactgct
     1861 agttgtggtg tagatttagc attctcaaac ccatgctgta gcgtttctaa tattgttaca
     1921 tagatctacc ggtgcctgtt aattgtattc gatcgggcgt ttctacatct gtccgcccac
     1981 ctagttttat atgtggtaat caaaattgcg ttgacttcgt gatgctgtct gtgtactgtt
     2041 tttaatcgct cttacttaga tgatcaacat ggtgatggtt acgatttact gttttctaat
     2101 ccctgttact tcgatgctgc agtttcagcc atgacaacag tattcagtcg actggtacca
     2161 acaggctcaa caatggtccg tcctgtagaa accccaaccc gtgaaatcaa aaaactcgac
     2221 ggcctgtggg cattcagtct ggatcgcgaa aactgtggaa ttgatcagcg ttggtgggaa
     2281 agcgcgttac aagaaagccg ggcaattgct gtgccaggca gttttaacga tcagttcgcc
     2341 gatgcagata ttcgtaatta tgcgggcaac gtctggtatc agcgcgaagt ctttataccg
     2401 aaaggttggg caggccagcg tatcgtgctg cgtttcgatg cggtcactca ttacggcaaa
     2461 gtgtgggtca ataatcagga agtgatggag catcagggcg gctatacgcc atttgaagcc
     2521 gatgtcacgc cgtatgttat tgccgggaaa agtgtacgta tcaccgtttg tgtgaacaac
     2581 gaactgaact ggcagactat cccgccggga atggtgatta ccgacgaaaa cggcaagaaa
     2641 aagcagtctt acttccatga tttctttaac tatgccggaa tccatcgcag cgtaatgctc
     2701 tacaccacgc cgaacacctg ggtggacgat atcaccgtgg tgacgcatgt cgcgcaagac
     2761 tgtaaccacg cgtctgttga ctggcaggtg gtggccaatg gtgatgtcag cgttgaactg
     2821 cgtgatgcgg atcaacaggt ggttgcaact ggacaaggca ctagcgggac tttgcaagtg
     2881 gtgaatccgc acctctggca accgggtgaa ggttatctct atgaactgtg cgtcacagcc
     2941 aaaagccaga cagagtgtga tatctacccg cttcgcgtcg gcatccggtc agtggcagtg
     3001 aagggcgaac agttcctgat taaccacaaa ccgttctact ttactggctt tggtcgtcat
     3061 gaagatgcgg acttgcgtgg caaaggattc gataacgtgc tgatggtgca cgaccacgca
     3121 ttaatggact ggattggggc caactcctac cgtacctcgc attaccctta cgctgaagag
     3181 atgctcgact gggcagatga acatggcatc gtggtgattg atgaaactgc tgctgtcggc
     3241 tttaacctct ctttaggcat tggtttcgaa gcgggcaaca agccgaaaga actgtacagc
     3301 gaagaggcag tcaacgggga aactcagcaa gcgcacttac aggcgattaa agagctgata
     3361 gcgcgtgaca aaaaccaccc aagcgtggtg atgtggagta ttgccaacga accggatacc
     3421 cgtccgcaag gtgcacggga atatttcgcg ccactggcgg aagcaacgcg taaactcgac
     3481 ccgacgcgtc cgatcacctg cgtcaatgta atgttctgcg acgctcacac cgataccatc
     3541 agcgatctct ttgatgtgct gtgcctgaac cgttattacg gatggtatgt ccaaagcggc
     3601 gatttggaaa cggcagagaa ggtactggaa aaagaacttc tggcctggca ggagaaactg
     3661 catcagccga ttatcatcac cgaatacggc gtggatacgt tagccgggct gcactcaatg
     3721 tacaccgaca tgtggagtga agagtatcag tgtgcatggc tggatatgta tcaccgcgtc
     3781 tttgatcgcg tcagcgccgt cgtcggtgaa caggtatgga atttcgccga ttttgcgacc
     3841 tcgcaaggca tattgcgcgt tggcggtaac aagaaaggga tcttcactcg cgaccgcaaa
     3901 ccgaagtcgg cggcttttct gctgcaaaaa cgctggactg gcatgaactt cggtgaaaaa
     3961 ccgcagcagg gaggcaaaca atcaggataa ctagctgagc ggccatgcta gagtccgcaa
     4021 aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga ataatgtgtg
     4081 agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt gttgagcata
     4141 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     4201 tcctaaaacc aaaatccagt gacctgtatg caactttatt atacaaagtt ggcattataa
     4261 aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     4321 ttatttggag ctccatggta gcgttaacgc ggccgcgata tcccctatag tgagtcgtat
     4381 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     4441 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     4501 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     4561 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     4621 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     4681 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     4741 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     4801 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     4861 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     4921 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     4981 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     5041 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     5101 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     5161 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     5221 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     5281 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     5341 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     5401 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     5461 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5521 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5581 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     5641 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5701 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5761 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5821 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
     5881 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5941 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6001 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6061 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6121 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6181 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6241 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6301 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     6361 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     6421 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     6481 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     6541 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     6601 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     6661 aaaacgacgg ccagtcttaa gctcgggccc tgcagctcta gagctcgaat tctacaggtc
     6721 actaatacca tctaagtagt tgatt
//
