LOCUS       pEN-L4-_pZmUBI-mScarlet-NLS-G7T-R1        5710 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...lv-eqt4mz7
VERSION     urn.local...lv-eqt4mz7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     attL4           1..97
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attL4"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:lr-eztz0t4"">attL4</a>"
                     /label="attL4"
     promoter        104..2090
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:thjac:1m-c4nnd54"">pZmUBIL</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/-1008;
                     ZmUBIL.2 (<a
                     href=""urn:local:lapau:v-c52za60"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     misc_feature    910
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     intron          1082..2091
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     motif           2128..2161
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="loxP"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:8a-c541szf"">loxP</a>"
                     /label="LoxP"
     CDS             2164..2859
                     /vntifkey=4
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mScarlet"
                     /Transferred_Similarity="100.00%"
                     /Adjusted_Transferred_Similarity="96.67%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/-24
                     (adjusted to 96.67%; Adjusted-Interval-0:0/0); mScarlet-L
                     (<a href=""urn:local:lapau:r-e4ew2m2"">mScarlet</a>)"
                     /label="mScarlet-L"
     NLS             2860..2880
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     terminator      2888..3159
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G7T"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1a-c4nnd53"">G7T</a>"
                     /label="G7T"
     attR1           3165..3321
                     /created_by="mamee"
                     /Color="#00cccc"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="attR1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:y-f0o11e8"">attR1</a>"
                     /label="attR1"
     primer          complement(3381..3401)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        3462..3512
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    3513..4322
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2o-c4nnd56"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4469..5057
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.49%"
                     /Primary_Match="99.49%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5656..5676
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
       61 tgctttttta taatgccaac tttgtataga aaagttgcta cctgtgcagc gtgacccggt
      121 cgtgcccctc tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata
      181 ttttttttgt cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact
      241 ttactctacg aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat
      301 ataaatgaac agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta
      361 cagttttatc tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat
      421 ataatacttc atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat
      481 agactaattt ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta
      541 aaactctatt ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg
      601 actaaaaatt aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg
      661 tttcgagtag ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca
      721 gcgaaccagc agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg
      781 cctctggacc cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc
      841 agaaattgcg tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc
      901 tcacggcacc ggcagctacg ggggattcct ttcccaccgc tccttcgctt tcccttcctc
      961 gcccgccgta ataaatagac accccctcca caccctcttt ccccaacctc gtgttgttcg
     1021 gagcgcacac acacacaacc agatctcccc caaatccacc cgtcggcacc tccgcttcaa
     1081 ggtacgccgc tcgtcctccc cccccccccc tctctacctt ctctagatcg gcgttccggt
     1141 ccatggttag ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg
     1201 ttagatccgt gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga
     1261 ttgctaactt gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag
     1321 acgggatcga tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc
     1381 tttatttcaa tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt
     1441 cttggttgtg atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattctgtt
     1501 tcaaactacc tggtggattt attaattttg gatctgtatg tgtgtgccat acatattcat
     1561 agttacgaat tgaagatgat ggatggaaat atcgatctag gataggtata catgttgatg
     1621 cgggttttac tgatgcatat acagagatgc tttttgttcg cttggttgtg atgatgtggt
     1681 gtggttgggc ggtcgttcat tcgttctaga tcggagtaga atactgtttc aaactacctg
     1741 gtgtatttat taattttgga actgtatgtg tgtgtcatac atcttcatag ttacgagttt
     1801 aagatggatg gaaatatcga tctaggatag gtatacatgt tgatgtgggt tttactgatg
     1861 catatacatg atggcatatg cagcatctat tcatatgctc taaccttgag tacctatcta
     1921 ttataataaa caagtatgtt ttataattat tttgatcttg atatacttgg atgatggcat
     1981 atgcagcagc tatatgtgga tttttttagc cctgccttca tacgctattt atttgcttgg
     2041 tactgtttct tttgtcgatg ctcaccctgt tgtttggtgt tacttctgca aacagtattc
     2101 agtcgactgg taccaacagg ctccatgata acttcgtata gcatacatta tacgaagtta
     2161 tccatggtga gcaagggcga ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg
     2221 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     2281 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttctcctgg
     2341 gacatcctgt cccctcagtt catgtacggc tccagggcct tcaccaagca ccccgccgac
     2401 atccccgact actataagca gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     2461 ttcgaggacg gcggcgccgt gaccgtgacc caggacacct ccctggagga cggcaccctg
     2521 atctacaagg tgaagctccg cggcaccaac ttccctcctg acggccccgt aatgcagaag
     2581 aagacaatgg gctgggaagc gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag
     2641 ggcgacatta agatggccct gcgcctgaag gacggcggcc gctacctggc ggacttcaag
     2701 accacctaca aggccaagaa gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag
     2761 ttggacatca cctcccacaa cgaggactac accgtggtgg aacagtacga acgctccgag
     2821 ggccgccact ccaccggcgg catggacgag ctgtacaagc ctaagaagaa gaggaaggtt
     2881 tgatcagccc cgatgagcta agctagctat atcatcaatt tatgtattac acataatatc
     2941 gcactcagtc tttcatctac ggcaatgtac cagctgatat aatcagttat tgaaatattt
     3001 ctgaatttaa acttgcatca ataaatttat gtttttgctt ggactataat acctgacttg
     3061 ttattttatc aataaatatt taaactatat ttctttcaag atgggaatta acatctacaa
     3121 attgcctttt cttatcgacc atgtactcga cggccgagtg tatgcaagtt tgtacaaaaa
     3181 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     3241 aaaacagact acataatact gtaaaacaca acatatgcag tcactatgaa tcaactactt
     3301 agatggtatt agtgacctgt agaattcgag ctctagagct gcagggcggc cgcgatatcc
     3361 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     3421 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
     3481 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
     3541 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     3601 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
     3661 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     3721 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     3781 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
     3841 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     3901 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     3961 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     4021 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
     4081 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     4141 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     4201 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     4261 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     4321 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     4381 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     4441 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4501 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4561 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     4621 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4681 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     4741 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     4801 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     4861 attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     4921 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     4981 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5041 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5101 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5161 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5221 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     5281 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     5341 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     5401 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     5461 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     5521 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     5581 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
     5641 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggcccgcg ttaacgctac
     5701 catggagctc
//
