LOCUS       pRA-U1-AG-U9           11405 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...3cv-ak3co46
VERSION     urn.local...3cv-ak3co46
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     Promoter        8..289
                     /Mismatches=0
                     /%_Identity=100
                     /Motif="CACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAG
                     TAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACG
                     GCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCC
                     TCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGC
                     AGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATC"
                     /X-Transferred_From="pGGZ004"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:maizel:2p5-ccnltde"">pGGZ004</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p3-ccnltde"">pR450</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:thjac:47-ckxljc3"">SpR</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a
                     href=""urn:local:maizel:2oq-ccnltdd"">pR451_pFASTR_A-G</a>
                     )"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:maizel:2p4-ccnltde"">pR449</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3jv-esp0psa"">pGG-A-PAgro1-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pAgro1 (<a
                     href=""urn:local:mamee:3k2-esp0psa"">pEN-L4-PAGRO1-SpCas9-
                     T3T-R1 </a>)"
                     /X-Alternative_Match="92.86%; Adjusted-Interval-0:12/-235;
                     Pc promoter (<a
                     href=""urn:local:mamee:405-esp0pt5"">pDEST32-GG</a>)"
                     /X-Alternative_Match="90.14%; Adjusted-Interval-0:0/0; P1
                     (<a href=""urn:local:mamee:3uc-esp0psx"">pGADT7-GG</a>)"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="96.81%"
                     /Primary_Match="96.81%; Adjusted-Interval-0:0/0; P1 (<a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>)"
                     /label="P1"
     misc_feature    290..1081
                     /note="Derived using Geneious Prime 2021.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="SpR"
                     /X-Transferred_Similarity="98.72%"
                     /X-Primary_Match="98.72%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8l-dgycryy"">SpR</a>"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1p-f0tk4mj"">SpR</a>"
                     /label="SpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1325..1913
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="99.83%"
                     /Primary_Match="99.83%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     regulatory      complement(1949..2209)
                     /%_Identity=100
                     /Motif="CGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAG
                     AGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATC
                     AGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGC
                     GAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAA
                     CGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG"
                     /annotation_group="pBR322: 7,710 -> 7,970"
                     /modified_by="User"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pBR322 bom"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7a-dlucaql"">pBR322 bom</a>"
                     /Alternative_Match="99.29%; Adjusted-Interval-0:-30/-150;
                     <a href=""urn:local:.:79-dlucaql"">bom</a>"
                     /label="pBR322 bom"
     rep_origin      2583..2777
                     /note="origin of replication fromÂ Pseudomonas pVS1
                     plasmid"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 oriV"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7t-dlucpl4"">pVS1 oriV</a>"
                     /label="pVS1 oriV"
     regulatory      complement(2843..3915)
                     /note="Pseudomonas pVS1 plasmid replication protein"
                     /product="replication protein RepA"
                     /gene="repA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 repA"
                     /Transferred_Similarity="99.91%"
                     /Primary_Match="99.91%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:24o-ciyzzla"">pVS1 repA</a>"
                     /label="pVS1 repA"
     regulatory      complement(4344..4973)
                     /note="Pseudomonas pVS1 plasmid stability protein"
                     /product="plasmid stability protein StaA"
                     /gene="staA"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pVS1 staA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:24p-ciyzzla"">pVS1 staA</a>"
                     /label="pVS1 staA"
     T-DNA_border    6129..6358
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RB"
                     /Transferred_Similarity="91.77%"
                     /Primary_Match="91.77%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6f-dgycryw"">RB</a>"
                     /label="RB"
     misc_feature    6366..6405
                     /created_by="lapau"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U1"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:63-cctbnu7"">U1</a>"
                     /label="U1"
                     /note="Geneious type: Golden Gibson Cloning site"
     misc_difference complement(6406..6423)
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7y-dgycryx"">I-SceI site</a>"
                     /label="I-SceI site"
     GG              complement(6424..6434)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2b8-ciz7x4i"">A</a>"
                     /label="A"
     CDS             complement(6441..6746)
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.35%"
                     /Primary_Match="99.35%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:t-cj5879l"">ccdB</a>"
                     /label="ccdB"
     UTR             complement(6747..7065)
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ccdB"
                     /Transferred_Similarity="99.37%"
                     /Primary_Match="99.37%; Adjusted-Interval-0:0/0; ccdB
                     5'UTR (<a href=""urn:local:thjac:t-cj5879l"">ccdB</a>)"
                     /label="ccdB 5'UTR"
     misc_feature    complement(7088..7747)
                     /Transferred_From="cmR"
                     /Transferred_Similarity="99.85%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="99.85%"
                     /Primary_Match="99.85%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:29-f0o4v7s"">CmR</a>"
                     /label="CmR"
                     /note="Geneious type: Bacterial Selection"
     promoter        complement(7801..7831)
                     /note="Lac promoter of Escherichia coli containing 2 base
                     pair mutations"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="lacUV5"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:1g0-c949y1m"">lacUV5</a>"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="CmR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; lacUV5
                     (<a href=""urn:local:.:29-f0o4v7s"">CmR</a>)"
                     /label="lacUV5"
     GG              7848..7858
                     /%_Identity=100
                     /Motif="GGTCTCAGTAT"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="G"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:86-dgycryy"">G</a>"
                     /label="G"
     misc_difference 7859..7876
                     /created_by="laurenspauwels"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="I-SceI site"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7y-dgycryx"">I-SceI site</a>"
                     /label="I-SceI site"
     misc_feature    7877..7916
                     /standard_name="U9"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="U9"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:7w-cctcf3v"">U9</a>"
                     /label="U9"
                     /note="Geneious type: Golden Gibson Cloning site"
     promoter        7958..8823
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pSbAHAS"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:7c-c53zgzm"">pSbAHAS</a>"
                     /label="pSbAHAS"
     CDS             8824..10740
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ZmHRA"
                     /Transferred_Similarity="99.95%"
                     /Primary_Match="99.95%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:lapau:6m-c53xbzf"">ZmHRA</a>"
                     /label="ZmHRA"
     terminator      10757..11067
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SbPinIIT"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; StPinII
                     (<a href=""urn:local:lapau:7f-c54071s"">SbPinIIT</a>)"
                     /label="StPinII"
     T-DNA_border    11076..11405
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="LB"
                     /Transferred_Similarity="98.80%"
                     /Primary_Match="98.80%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:6e-dgycryw"">LB</a>"
                     /label="LB"
ORIGIN      
        1 attcgggcac gaacccagtg gacataagcc tcgttcggtt cgtaagctgt aatgcaagta
       61 gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      121 gcagtggcgg ttttcatggc ttcttgttat gacatgtttt tttggggtac agtctatgcc
      181 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      241 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
      301 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
      361 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
      421 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
      481 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
      541 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
      601 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
      661 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
      721 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
      781 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
      841 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
      901 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
      961 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1021 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     1081 atgtctagct agaaattcgt tcaagccgac gccgcttcgc cggcgttaac tcaagcgatt
     1141 agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta
     1201 tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa
     1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2161 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     2221 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2341 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     2401 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     2461 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     2521 agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     2581 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     2641 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     2701 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     2761 tgtacgtgct atccacagga aagagagctt ttcgaccttt ttcccctgct agggcaattt
     2821 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     2881 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     2941 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     3001 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     3061 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     3121 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     3181 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     3241 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     3301 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     3361 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     3421 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     3481 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     3541 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     3601 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     3661 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     3721 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     3781 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     3841 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     3901 gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     3961 cagacaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     4021 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     4081 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     4141 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     4201 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     4261 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     4321 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     4381 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     4441 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     4501 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     4561 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     4621 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     4681 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     4741 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     4801 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
     4861 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
     4921 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
     4981 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
     5041 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
     5101 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
     5161 gcacggttga gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
     5221 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
     5281 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
     5341 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
     5401 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
     5461 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
     5521 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
     5581 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
     5641 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
     5701 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
     5761 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
     5821 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
     5881 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
     5941 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
     6001 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
     6061 aacttaggac ttgtgcgaca tgtcgttttc agaagagggc tgcactgaac gtcagaagcc
     6121 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
     6181 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     6241 ccgttattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
     6301 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctaagcttg
     6361 agctccatta ctcgcatcca ttctcaggct gtctcgtctc gtctctaggg ataacagggt
     6421 aataccttga gaccgtcgac ttatattccc cagaacatca ggttaatggc gtttttgatg
     6481 tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac tggcacactg
     6541 gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt
     6601 tcacgggaga ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc
     6661 ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt
     6721 ttataggtgt aaaccttaaa ctgcatttca ccagtccctg ttctcgtcag caaaagagcc
     6781 gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg
     6841 ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccagac cggagatatt
     6901 gacatcatat atgccttgag caactgatag ctgtcgctgt caactgtcac tgtaatacgc
     6961 tgcttcatag cacacctctt tttgacatac ttcgggtata catatcagta tatattctta
     7021 taccgcaaaa atcagcgcgc aaatacgcat actgttatct ggcttttagt aagccggatc
     7081 cacgcgttta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     7141 tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca
     7201 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     7261 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
     7321 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
     7381 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
     7441 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
     7501 ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa
     7561 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
     7621 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
     7681 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
     7741 tctccatttt agcttcctta gctcctgaaa atctcgccgg atcctaactc aaaatccaca
     7801 cattatacga gccggaagca taaagtgtaa agcctggggt gcctaatggt ctcagtatat
     7861 taccctgtta tccctagttc cttatcatct ggcgaatcgg acccacaaga gcactggggc
     7921 cctcttagag gatccccggg taccgagctc ggatatctac tttaacgcct ctaaccaaca
     7981 cccctttatc tttataagga acaataaaca gaatttgccc cactgttcta aatcacctaa
     8041 taatatcccc agctaaaaac aataaaggtt tcctagaatt aagacaagca tgactgttcc
     8101 tccaggaggg tttggaacat tgttgcagtc ttgcagatac gggcgaaggg tgagaaacag
     8161 agcggagggc tggaggtgac ctcggtagtc gacgccggag ttgagcttga caacgacggg
     8221 gcggcccctg atggacttga ggaagtccga tggcgtctac accgtcccgc cggcgcccga
     8281 ggcgggcctg tcgctgccgc cgccgccgct gctcatcttg cgcgctgtgc ccccggcggt
     8341 gtccctgtgt tgcggatcgc gggtgggcca ggtggatgcg agggcgaccc gtttggactc
     8401 cggccggagc cgccggatcc ctggtcggtg tcagtgccgt ttactctggg ccgcacgtgt
     8461 cagtaccgtc tgtagatgac aacaacccgt cgtccacagt catgtccaaa atatcctttc
     8521 ttcttttttt tcgattcgga tatgtatctt cctttttttt ttccaaaaat cttcttgacg
     8581 caccagcgcg cacgtttgtg gtaaacgccg acacgtcggt cccacgtcga tagaccccac
     8641 ccaccagtga gtagcgtgta cgtattcggg ggtgacggac gtgtcgccgt cgtcttgcta
     8701 gtcccattcc catctgagcc acacatctct gaacaaaaaa aaggagggag gcctccacgc
     8761 acatccccct ccgtgccacc cgccccaaac cctcgcgccg cctccgagac agccgccgca
     8821 accatggcca ccgccgccgc cgcgtctacc gcgctcactg gcgccactac cgctgcgccc
     8881 aaggcgaggc gccgggcgca cctcctggcc acccgccgcg ccctcgccgc gcccatcagg
     8941 tgctcagcgg cgtcacccgc catgccgatg gctcccccgg ccaccccgct ccggccgtgg
     9001 ggacccaccg agccccgcaa gggtgctgac atcctcgtcg agtccctcga gcgctgcggc
     9061 gtccgcgacg tctttgccta ccccggcggc gcgtccatgg agatccacca ggcactcacc
     9121 cgctcccccg tcatcgccaa ccacctcttc cgccacgagc aaggggaggc ctttgccgcc
     9181 tccggctacg cgcgctcctc gggccgcgtc ggcgtctgca tcgccacctc cggccccggc
     9241 gccaccaacc tagtctccgc gctcgccgac gcgctgctcg attccgtccc catggtcgcc
     9301 atcacgggac aggtggcgcg acgcatgatt ggcaccgacg ccttccagga gactcccatc
     9361 gtcgaggtca cccgctccat caccaagcac aactacctgg tcctcgacgt cgacgacatc
     9421 ccccgcgtcg tgcaggaggc tttcttcctc gcctcctctg gtcgaccagg gccggtgctt
     9481 gtcgacatcc ccaaggacat ccagcagcag atggcggtgc ctgtctggga caagcccatg
     9541 agtctgcctg ggtacattgc gcgccttccc aagccccctg cgactgagtt gcttgagcaa
     9601 gtgctgcgtc ttgttggtga atcgcggcgc cctgttcttt atgtgggcgg tggctgcgca
     9661 gcatctggtg aggagttgcg acgctttgtg gagctgactg gaatcccggt cacaactact
     9721 cttatgggcc tcggcaactt ccccagcgac gacccactgt ctctgcgcat gctaggtatg
     9781 catgggacgg tgtatgcaaa ttatgcagtg gataaggccg atctgttgct tgcacttggt
     9841 gtgcggtttg atgatcgcgt gacagggaag attgaggctt ttgcaagcag ggctaagatt
     9901 gtgcacgttg atattgatcc ggctgagatt ggcaagaaca agcagccaca tgtgtccatc
     9961 tgtgcagatg ttaaacttgc tttgcagggc atgaatgctc ttcttgaagg aagcacatca
    10021 aagaagagct ttgactttgg ctcatggaac gatgagttgg atcagcagaa gagggaattc
    10081 ccccttgggt ataaaacatc taatgaggag atccagccac aatatgctat tcaggttctt
    10141 gatgagctga cgaaaggcga ggccatcatc ggcacaggtg ttgggcagca ccagatgtgg
    10201 gcggcacagt actacactta caagcggcca aggcagtggt tgtcctcagc tggtcttggg
    10261 gctatgggat ttggtttgcc ggctgctgct ggtgcttctg tggcaaaccc aggtgtcact
    10321 gttgttgaca tcgatggaga tggtagcttt ctcatgaacg ttcaggagct agctatgatc
    10381 cgaattgaga acctcccagt gaaggtcttt gtgctaaaca accagcacct ggggatggtg
    10441 gtgcagttgg aggacaggtt ctataaggcc aacagagcgc acacatactt gggaaaccca
    10501 gagaatgaaa gtgagatata tccagatttc gtgacgatcg ccaaagggtt caacattcca
    10561 gcggtccgtg tgacaaagaa gaacgaagtc cgcgcagcga taaagaagat gctcgagact
    10621 ccagggccgt acctcttgga tataatcgtc ccacaccagg agcatgtgtt gcctatgatc
    10681 cctagtggtg gggctttcaa ggatatgatc ctggatggtg atggcaggac tgtgtactga
    10741 ctagctagtc agttaaccta gacttgtcca tcttctggat tggccaactt aattaatgta
    10801 tgaaataaaa ggatgcacac atagtgacat gctaatcact ataatgtggg catcaaagtt
    10861 gtgtgttatg tgtaattact agttatctga ataaaagaga aagagatcat ccatatttct
    10921 tatcctaaat gaatgtcacg tgtctttata attctttgat gaaccagatg catttcatta
    10981 accaaatcca tatacatata aatattaatc atatataatt aatatcaatt gggttagcaa
    11041 aacaaatcta gtctaggtgt gttttgcgaa gatatccaat tcggcgttaa ttcagtacat
    11101 taacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat
    11161 cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga
    11221 tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac
    11281 tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca
    11341 cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt
    11401 gatca
//
