LOCUS       pGON005_pZmUBI-EvoFerny-nCas9-3xSV40NLS-G7T       11639 bp    DNA     circular UNA 08-SEP-2022
DEFINITION  .
ACCESSION   urn.local...di-en4mbdu
VERSION     urn.local...di-en4mbdu
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..864
                     /regulatory_class="promoter"
                     /gene="CaMV 35S"
                     /note="internal BsaI recognition site removed"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:6v-dgycryx"">pCaMV 35S</a>)"
                     /label="p35S"
     CDS             899..1606
                     /created_by="User"
                     /modified_by="User"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:3a-c4nnd56"">mCherry</a>"
                     /label="mCherry"
     Linker          1610..1657
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker
                     (<a href=""urn:local:thjac:1g-c4nnd54"">(GS)8</a>)"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             1658..1906
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      1914..2181
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /label="NOST"
     promoter        2210..4195
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/1;
                     ZmUBIL.2 (<a
                     href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     intron          3187..4196
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     CDS             4203..4685
                     /created_by="chgai"
                     /label="EvoFERNY"
     Linker          4686..4733
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="32-aa linker"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; XTEN (<a
                     href=""urn:local:.:a3-dgycryz"">32-aa linker</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a5-dgycryz"">XTEN</a>"
                     /label="XTEN"
     CDS             4740..8843
                     /X-Transferred_From="Cas9PTA"
                     /X-Transferred_Similarity="99.98%"
                     /X-Primary_Match="99.98%; Adjusted-Interval-0:0/0; CAS9
                     (<a href=""urn:local:thjac:3c-c4nnd56"">Cas9PTA</a>)"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="nCAS9(D10A)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:pe-ecpo7bv"">nCAS9(D10A)</a>"
                     /label="nCAS9(D10A)"
     NLS             8850..8936
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3xSV40"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:5q-ecucp4e"">3xSV40</a>"
                     /modified_by="mamee"
                     /label="3xSV40"
     terminator      8944..9215
                     /label="G7T"
     Linker          9220..9268
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(9329..9349)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        9410..9460
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    9461..10270
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      10417..11005
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          11604..11624
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ccgatccatt cggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
       61 tggttagaga ggcttacgca gcacgtctca tcaagacgat ctacccgagc aataatctcc
      121 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
      181 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
      241 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
      301 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
      361 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
      421 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
      481 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
      541 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
      601 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
      661 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      721 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      781 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      841 tggagagaac acgggggacg agctaacagt attcagtcga ctggtaccaa caggctccat
      901 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      961 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1021 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1081 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1141 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1201 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     1261 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1321 gcagaagaag acgatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1381 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1441 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1501 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     1561 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtcag gttctggttc
     1621 aggttcaggt tctggatctg gaagtggatc tggatcagcg gccgcatctg aattcaagcg
     1681 tgaagagcaa gcaaggaaag ctaaggtgaa caatgagaaa aagacggaaa tagtgaaacc
     1741 agagagttgt agcaatgaag gagatgtcaa ggatctgaaa agaaaggact ctgaggatgg
     1801 aaacgagggt gaggaagaag aagcttcttc gaaaccgaaa aagccaaaag ttgctctttc
     1861 tcatcttcag gacattgacg acacagaagc tgatcaagaa gaagagtaac tgcctagagt
     1921 caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     1981 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     2041 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     2101 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     2161 tcatctatgt tactagatcg aactataagc taggtagtaa ctagtacctg tgcagcgtga
     2221 cccggtcgtg cccctctcta gagataatga gcattgcatg tctaagttat aaaaaattac
     2281 cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta tacatatatt
     2341 taaactttac tctacgaata atataatcta tagtactaca ataatatcag tgttttagag
     2401 aatcatataa atgaacagtt agacatggtc taaaggacaa ttgagtattt tgacaacagg
     2461 actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc aaatagcttc
     2521 acctatataa tacttcatcc attttattag tacatccatt tagggtttag ggttaatggt
     2581 ttttatagac taattttttt agtacatcta ttttattcta ttttagcctc taaattaaga
     2641 aaactaaaac tctattttag tttttttatt taataattta gatataaaat agaataaaat
     2701 aaagtgacta aaaattaaac aaataccctt taagaaatta aaaaaactaa ggaaacattt
     2761 ttcttgtttc gagtagataa tgccagcctg ttaaacgccg tcgacgagtc taacggacac
     2821 caaccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg
     2881 tcgctgcctc tggacccctc tcgagagttc cgctccaccg ttggacttgc tccgctgtcg
     2941 gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg cggcctcctc
     3001 ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct
     3061 tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt
     3121 gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc
     3181 ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt
     3241 ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt
     3301 ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt
     3361 tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc
     3421 cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct
     3481 tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt
     3541 tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt
     3601 ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata
     3661 ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt
     3721 tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat
     3781 gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact
     3841 acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg
     3901 agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac
     3961 tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct
     4021 atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat
     4081 ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg
     4141 cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt ctgcaaacaa
     4201 tgtttgagag gaactacgac ccccgggagc tgagaaagga gacatacctg ctgtatgaga
     4261 tcaagtgggg caagtccggc aagctgtgga ggcactggtg ccagaacaat cgcacacagc
     4321 acgccgaggt gtacttcctg gagaacatct ttaatgcccg gagattcaat ccatctaccc
     4381 actgtagcat cacatggtat ctgagctggt ccccctgcgc cgagtgttct cagaagatcg
     4441 tggatttcct gaaggagcac cctaacgtga atctggagat ctatgtggcc cggctgtact
     4501 atccagagaa cgagaggaat aggcagggcc tgcgggatct ggtgaattcc ggcgtgacca
     4561 tcagaatcat ggacctgcca gattacaact attgctggaa gaccttcgtg agcgatcagg
     4621 gaggcgacga ggattactgg ccaggacact tcgccccttg gatcaagcag tatagcctga
     4681 agctgtctgg ttctgaaact cctggaactt cagaatctgc tactcctgaa tccggctcga
     4741 tggataagaa gtactctatc ggactcgcta tcggaactaa ctctgtggga tgggctgtga
     4801 tcaccgatga gtacaaggtg ccatctaaga agttcaaggt tctcggaaac accgataggc
     4861 actctatcaa gaaaaacctt atcggtgctc tcctcttcga ttctggtgaa actgctgagg
     4921 ctaccagact caagagaacc gctagaagaa ggtacaccag aagaaagaac aggatctgct
     4981 acctccaaga gatcttctct aacgagatgg ctaaagtgga tgattcattc ttccacaggc
     5041 tcgaagagtc attcctcgtg gaagaagata agaagcacga gaggcaccct atcttcggaa
     5101 acatcgttga tgaggtggca taccacgaga agtaccctac tatctaccac ctcagaaaga
     5161 agctcgttga ttctactgat aaggctgatc tcaggctcat ctacctcgct ctcgctcaca
     5221 tgatcaagtt cagaggacac ttcctcatcg agggtgatct caaccctgat aactctgatg
     5281 tggataagtt gttcatccag ctcgtgcaga cctacaacca gcttttcgaa gagaacccta
     5341 tcaacgcttc aggtgtggat gctaaggcta tcctctctgc taggctctct aagtcaagaa
     5401 ggcttgagaa cctcattgct cagctccctg gtgagaagaa gaacggactt ttcggaaact
     5461 tgatcgctct ctctctcgga ctcaccccta acttcaagtc taacttcgat ctcgctgagg
     5521 atgcaaagct ccagctctca aaggatacct acgatgatga tctcgataac ctcctcgctc
     5581 agatcggaga tcagtacgct gatttgttcc tcgctgctaa gaacctctct gatgctatcc
     5641 tcctcagtga tatcctcaga gtgaacaccg agatcaccaa ggctccactc tcagcttcta
     5701 tgatcaagag atacgatgag caccaccagg atctcacact tctcaaggct cttgttagac
     5761 agcagctccc agagaagtac aaagagattt tcttcgatca gtctaagaac ggatacgctg
     5821 gttacatcga tggtggtgca tctcaagaag agttctacaa gttcatcaag cctatcctcg
     5881 agaagatgga tggaaccgag gaactcctcg tgaagctcaa tagagaggat cttctcagaa
     5941 agcagaggac cttcgataac ggatctatcc ctcatcagat ccacctcgga gagttgcacg
     6001 ctatccttag aaggcaagag gatttctacc cattcctcaa ggataacagg gaaaagattg
     6061 agaagattct caccttcaga atcccttact acgtgggacc tctcgctaga ggaaactcaa
     6121 gattcgcttg gatgaccaga aagtctgagg aaaccatcac cccttggaac ttcgaagagg
     6181 tggtggataa gggtgctagt gctcagtctt tcatcgagag gatgaccaac ttcgataaga
     6241 accttccaaa cgagaaggtg ctccctaagc actctttgct ctacgagtac ttcaccgtgt
     6301 acaacgagtt gaccaaggtt aagtacgtga ccgagggaat gaggaagcct gcttttttgt
     6361 caggtgagca aaagaaggct atcgttgatc tcttgttcaa gaccaacaga aaggtgaccg
     6421 tgaagcagct caaagaggat tacttcaaga aaatcgagtg cttcgattca gttgagattt
     6481 ctggtgttga ggataggttc aacgcatctc tcggaaccta ccacgatctc ctcaagatca
     6541 ttaaggataa ggatttcttg gataacgagg aaaacgagga tatcttggag gatatcgttc
     6601 ttaccctcac cctctttgaa gatagagaga tgattgaaga aaggctcaag acctacgctc
     6661 atctcttcga tgataaggtg atgaagcagt tgaagagaag aagatacact ggttggggaa
     6721 ggctctcaag aaagctcatt aacggaatca gggataagca gtctggaaag acaatccttg
     6781 atttcctcaa gtctgatgga ttcgctaaca gaaacttcat gcagctcatc cacgatgatt
     6841 ctctcacctt taaagaggat atccagaagg ctcaggtttc aggacagggt gatagtctcc
     6901 atgagcatat cgctaacctc gctggatctc ctgcaatcaa gaagggaatc ctccagactg
     6961 tgaaggttgt ggatgagttg gtgaaggtga tgggaaggca taagcctgag aacatcgtga
     7021 tcgaaatggc tagagagaac cagaccactc agaagggaca gaagaactct agggaaagga
     7081 tgaagaggat cgaggaaggt atcaaagagc ttggatctca gatcctcaaa gagcaccctg
     7141 ttgagaacac tcagctccag aatgagaagc tctacctcta ctacctccag aacggaaggg
     7201 atatgtatgt ggatcaagag ttggatatca acaggctctc tgattacgat gttgatcata
     7261 tcgtgccaca gtcattcttg aaggatgatt ctatcgataa caaggtgctc accaggtctg
     7321 ataagaacag gggtaagagt gataacgtgc caagtgaaga ggttgtgaag aaaatgaaga
     7381 actattggag gcagctcctc aacgctaagc tcatcactca gagaaagttc gataacttga
     7441 ctaaggctga gaggggagga ctctctgaat tggataaggc aggattcatc aagaggcagc
     7501 ttgtggaaac caggcagatc actaagcacg ttgcacagat cctcgattct aggatgaaca
     7561 ccaagtacga tgagaacgat aagttgatca gggaagtgaa ggttatcacc ctcaagtcaa
     7621 agctcgtgtc tgatttcaga aaggatttcc aattctacaa ggtgagggaa atcaacaact
     7681 accaccacgc tcacgatgct taccttaacg ctgttgttgg aaccgctctc atcaagaagt
     7741 atcctaagct cgagtcagag ttcgtgtacg gtgattacaa ggtgtacgat gtgaggaaga
     7801 tgatcgctaa gtctgagcaa gagatcggaa aggctaccgc taagtatttc ttctactcta
     7861 acatcatgaa tttcttcaag accgagatta ccctcgctaa cggtgagatc agaaagaggc
     7921 cactcatcga gacaaacggt gaaacaggtg agatcgtgtg ggataaggga agggatttcg
     7981 ctaccgttag aaaggtgctc tctatgccac aggtgaacat cgttaagaaa accgaggtgc
     8041 agaccggtgg attctctaaa gagtctatcc tccctaagag gaactctgat aagctcattg
     8101 ctaggaagaa ggattgggac cctaagaaat acggtggttt cgattctcct accgtggctt
     8161 actctgttct cgttgtggct aaggttgaga agggaaagag taagaagctc aagtctgtta
     8221 aggaacttct cggaatcact atcatggaaa ggtcatcttt cgagaagaac ccaatcgatt
     8281 tcctcgaggc taagggatac aaagaggtta agaaggatct catcatcaag ctcccaaagt
     8341 actcactctt cgaactcgag aacggtagaa agaggatgct cgcttctgct ggtgagcttc
     8401 aaaagggaaa cgagcttgct ctcccatcta agtacgttaa ctttctttac ctcgcttctc
     8461 actacgagaa gttgaaggga tctccagaag ataacgagca gaagcaactt ttcgttgagc
     8521 agcacaagca ctacttggat gagatcatcg agcagatctc tgagttctct aaaagggtga
     8581 tcctcgctga tgcaaacctc gataaggtgt tgtctgctta caacaagcac agagataagc
     8641 ctatcaggga acaggcagag aacatcatcc atctcttcac ccttaccaac ctcggtgctc
     8701 ctgctgcttt caagtacttc gatacaacca tcgataggaa gagatacacc tctaccaaag
     8761 aagtgctcga tgctaccctc atccatcagt ctatcactgg actctacgag actaggatcg
     8821 atctctcaca gctcggtggt gattcaggtc caaagaagaa gcggaaggtg tctggaggtt
     8881 ctcctaagaa aaagagaaaa gtgtccggcg gctccccgaa gaagaagcgc aaggtgtaac
     8941 tgcccccgat gagctaagct agctatatca tcaatttatg tattacacat aatatcgcac
     9001 tcagtctttc atctacggca atgtaccagc tgatataatc agttattgaa atatttctga
     9061 atttaaactt gcatcaataa atttatgttt ttgcttggac tataatacct gacttgttat
     9121 tttatcaata aatatttaaa ctatatttct ttcaagatgg gaattaacat ctacaaattg
     9181 ccttttctta tcgaccatgt actcgacggc cgagtactat aagctaggta gtaactagtg
     9241 cgactgtcta gaatcgcgta tgcgagtgta tctagaatcg cgtatgcgag tgtatgagct
     9301 cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     9361 cgtgtctcaa aatctctgat gttacattgc acaagataaa ataatatcat catgaacaat
     9421 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     9481 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     9541 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     9601 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     9661 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     9721 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     9781 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     9841 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     9901 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     9961 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
    10021 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
    10081 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
    10141 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
    10201 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
    10261 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
    10321 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
    10381 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10441 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10501 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10561 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10621 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10681 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10741 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10801 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    10861 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    10921 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    10981 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    11041 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    11101 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    11161 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
    11221 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    11281 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
    11341 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
    11401 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
    11461 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gacttccgac
    11521 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
    11581 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggcccacct
//
