LOCUS       pGG-D-PmCDA1-E          3603 bp    DNA     circular     21-APR-2023
DEFINITION  .
ACCESSION   urn.local...6d-a58fqd2
VERSION     urn.local...6d-a58fqd2
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2j-c4nnd55"">D</a>"
                     /label="D"
     Linker          14..205
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="64-aa linker"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a4-dgycryz"">64-aa linker</a>"
                     /label="64-aa linker"
     tag             206..271
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /modified_by="mamee"
                     /label="3xFLAG"
     CDS             278..901
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGE
                     RRACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAE
                     KILEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCR
                     KIFIQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
                     /label="PmCDA1"
     NLS             905..925
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     GG              complement(929..939)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="E"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:29v-ciz7957"">E</a>"
                     /label="E"
     primer          complement(959..979)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1459..1563
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1564..2424
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2595..3183
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3547..3568
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcatca gctggaggag ggccaggagc agagtatgtt agagcgttgt ttgattttaa
       61 cggtaacgat gaagaggatt taccctttaa gaaaggcgac attctcagga ttagggataa
      121 acctgaagag caatggtgga atgctgagga tagtgaaggc aaacgaggaa tgattttagt
      181 gccgtatgtg gagaaatatt cgggtgacta caaagatcat gatggtgatt acaaagacca
      241 tgacatcgac tacaaggatg atgatgataa gtcagggatg acagatgctg aatatgtcag
      301 aatccacgaa aagttggaca tttacacgtt taagaagcag ttcttcaaca acaagaaatc
      361 tgtttcgcat aggtgctatg tgcttttcga actaaaacgt cgtggagaaa gacgggcttg
      421 cttttggggt tacgcggtta acaaaccaca atcaggtact gaacgaggaa tacacgctga
      481 aatcttttct atccgaaagg ttgaggaata tctacgtgac aatcctggac agttcactat
      541 caattggtat tctagctggt caccatgtgc agattgtgct gagaagattc tcgaatggta
      601 caatcaagag cttagaggca atggacatac attgaaaata tgggcatgca agctctacta
      661 cgaaaagaat gccagaaacc aaattgggct ttggaacttg agggataatg gagttgggct
      721 taatgtcatg gtttctgagc actatcaatg ttgtcggaag atcttcatac aaagttccca
      781 taaccagttg aatgagaaca gatggttaga gaaaaccctt aaaagagccg agaagagaag
      841 atccgaactg agcattatga tacaggtcaa aattctgcat accactaaga gtccagctgt
      901 aggtcctaag aagaaacgta aagtatgact gctgagaccg aattctcgcc ctatagtgag
      961 tcgtattacc tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     1021 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     1081 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     1141 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     1201 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     1261 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     1321 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     1381 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     1441 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1501 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1561 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1621 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1681 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1741 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1801 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1861 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1921 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1981 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     2041 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     2101 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     2161 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     2221 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggc
     2281 tctcgaggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     2341 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2401 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     2461 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2521 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2581 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2641 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2701 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2761 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2821 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2881 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2941 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3001 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3061 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3121 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3181 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3241 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3301 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3361 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3421 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     3481 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     3541 ggaattgtga gcggataaca atttcacacg agctatttag gtgacactat agaagtgaag
     3601 ctt
//
