LOCUS       pGG-B-SV40-NLS-C        2722 bp    DNA     circular UNA 08-SEP-2022
DEFINITION  .
ACCESSION   p00825
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     NLS             12..47
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     GG              complement(48..58)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(78..98)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        578..682
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    683..1543
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1714..2302
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2666..2687
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatgtcaagg gctgatccta agaagaagag gaaggttggc ttgagaccga
       61 attctcgccc tatagtgagt cgtattacct ggccgtcgtt ttacaacgtc gtgactggga
      121 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
      181 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
      241 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
      301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
      361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
      421 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
      481 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      541 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      661 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      721 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      781 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      841 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      901 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      961 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1021 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1081 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1141 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1201 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1261 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1321 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1381 tggagccggt gagcgtggct ctcgaggtat cattgcagca ctggggccag atggtaagcc
     1441 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1501 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1561 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1621 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1681 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1741 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1801 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1861 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1921 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1981 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2041 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2101 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2161 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2221 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2281 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2341 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2401 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     2461 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     2521 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     2581 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     2641 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacga gctatttagg
     2701 tgacactata gaagtgaagc tt
//
