LOCUS       pGG-B-nucNLS-C          2743 bp    DNA     circular UNA 09-SEP-2022
DEFINITION  .
ACCESSION   p00950
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     NLS             12..68
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS Nucleoplasmin"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Nucleoplasmin (<a href=""urn:local:.:9z-dgycryz"">NLS
                     Nucleoplasmin</a>)"
                     /label="Nucleoplasmin"
     GG              complement(69..79)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(99..119)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        599..703
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    704..1564
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      1735..2323
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          2687..2708
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatgaagagg cctgcagcca ccaaaaaggc aggccaggca aagaagaaga
       61 aacttgacgg cttgagaccg aattctcgcc ctatagtgag tcgtattacc tggccgtcgt
      121 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      181 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      241 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      301 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      361 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      421 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      481 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      541 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      601 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      661 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      721 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      781 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      841 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      901 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      961 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1021 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1081 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1141 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1201 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1261 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1321 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1381 ctggtttatt gctgataaat ctggagccgg tgagcgtggc tctcgaggta tcattgcagc
     1441 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1501 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1561 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1621 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1681 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1741 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1801 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1861 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     1921 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1981 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2041 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2101 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2161 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2221 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2281 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2341 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2401 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2461 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     2521 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2581 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     2641 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     2701 atttcacacg agctatttag gtgacactat agaagtgaag ctt
//
