LOCUS       pGG-B-A3B-intron-XTEN-C        3499 bp    DNA     circular     09-SEP-2022
DEFINITION  .
ACCESSION   urn.local...12j-agn5xh6
VERSION     urn.local...12j-agn5xh6
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     CDS             join(12..296,485..776)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /label="APOBEC3B"
     intron          296..484
                     /vntifkey=15
                     /modified_by="thjac"
                     /label="IV2 intron"
     Linker          777..824
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="32-aa linker"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; XTEN (<a
                     href=""urn:local:.:a3-dgycryz"">32-aa linker</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a5-dgycryz"">XTEN</a>"
                     /label="XTEN"
     GG              complement(825..835)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(855..875)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1355..1459
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1460..2320
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2491..3079
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3443..3464
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatggagatc ctgagatacc tgatggaccc cgacaccttc accttcaatt
       61 tcaacaacga ccctctggtg ctgcggcgga gacagaccta cctgtgttac gaggtggaac
      121 ggctggacaa cggcacctgg gttctgatgg atcagcacat gggctttctg tgcaacgagg
      181 ccaagaacct gctgtgcggc ttctatggca gacacgccga gctgcggttt ctggatctgg
      241 tgccttctct gcagctggac cccgctcaga tctacagagt gacctggttc atcaggtaag
      301 tttctgcttc tacctttgat atatatataa taattatcat taattagtag taatataata
      361 tttcaaatat ttttttcaaa ataaaagaat gtagtatata gcaattgctt ttctgtagtt
      421 tataagtgtg tatattttaa tttataactt ttctaatata tgaccaaaat ttgttgatgt
      481 gcagctggtc cccatgcttc tcttggggct gtgctggcga agtgcgggcc ttcctgcaag
      541 agaatactca cgtgcggctg cggatcttcg ccgccagaat ctacgactac gatcccctgt
      601 acaaagaggc cctgcagatg ctgagagatg ccggtgctca ggtgtccatc atgacctacg
      661 acgagttcga gtactgctgg gacaccttcg tgtacagaca gggctgccct ttccagcctt
      721 gggacggact ggaagaacac tctcaggctc tgagcggcag actgagagcc attctttctg
      781 gttctgaaac tcctggaact tcagaatctg ctactcctga atccggcttg agaccgaatt
      841 ctcgccctat agtgagtcgt attacctggc cgtcgtttta caacgtcgtg actgggaaaa
      901 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      961 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1021 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     1081 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     1141 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     1201 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     1261 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     1321 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1381 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     1441 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     1501 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     1561 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     1621 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     1681 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     1741 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     1801 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1861 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1921 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1981 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     2041 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     2101 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     2161 agccggtgag cgtggctctc gaggtatcat tgcagcactg gggccagatg gtaagccctc
     2221 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     2281 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     2341 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     2401 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2881 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3121 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3181 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3241 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     3301 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     3361 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     3421 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacgagct atttaggtga
     3481 cactatagaa gtgaagctt
//
