LOCUS       pZmUBI-BP-A3A-intron-6xGGGGS-TaLbCas12a(D156R-D832A)-nucNLS-UGI-SV40-P2A-GFP-N7-NLS-G7T       10741 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...da-en4mbds
VERSION     urn.local...da-en4mbds
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1986
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/1;
                     ZmUBIL.2 (<a
                     href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     intron          978..1987
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     NLS             1994..2044
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     CDS             join(2069..2355,2545..2851)
                     /codon_start=1
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A3Aintron"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; APOBEC3A (<a
                     href=""urn:local:thjac:t-ecoeihu"">A3Aintron</a>)"
                     /label="APOBEC3A"
     intron          2356..2544
                     /vntifkey=15
                     /modified_by="thjac"
                     /label="IV2 intron"
     CDS             2852..2941
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="(GGGS)6"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:4k-ecylg1o"">(GGGS)6</a>"
                     /modified_by="mamee"
                     /label="6xGGGS"
     CDS             2948..6631
                     /%_Identity=100
                     /Motif="ATGAGCAAGCTGGAGAAGTTCACCAACTGCTACTCACTCTCCAAGACCCT
                     CCGCTTCAAGGCCATCCCCGTCGGCAAGACCCAGGAGAACATCGACAACAAGAGGCTG
                     CTGGTGGAGGATGAGAAGAGGGCCGAGGACTACAAGGGCGTCAAGAAGCTCCTCGACC
                     GGTACTACCTCTCATTCATCAATGATGTCCTCCACTCCATCAAGCTGAAGAACCTCAA
                     CAACTACATCTCCCTCTTCCGCAAGAAGACAAGGACCGAGAAGGAGAACAAGGAGCTG
                     GAGAACCTGGAGATCAACCTCCGCAAGGAGATCGCCAAGGCCTTCAAGGGCAACGAGG
                     GCTACAAGAGCCTCTTCAAGAAGGACATCATCGAGACGATCCTACCGGAGTTCCTGGA
                     TGACAAGGACGAGATCGCCCTGGTGAACAGCTTCAACGGCTTCACCACCGCCTTCACC
                     GGCTTCTTCCGCAACCGGGAGAACATGTTCTCCGAGGAGGCCAAGAGCACCTCCATCG
                     CCTTCAGGTGCATCAACGAGAACCTCACCCGCTACATCTCAAACATGGACATCTTCGA
                     GAAGGTGGACGCCATCTTCGACAAGCATGAGGTCCAGGAGATCAAGGAGAAGATCCTG
                     AACTCCGACTACGACGTGGAGGACTTCTTCGAGGGCGAGTTCTTCAACTTCGTCCTGA
                     CCCAAGAAGGCATCGACGTCTACAACGCCATCATCGGCGGCTTCGTCACCGAGTCAGG
                     TGAGAAGATCAAGGGCCTCAACGAGTACATCAACCTCTACAACCAGAAGACCAAGCAG
                     AAGCTCCCCAAGTTCAAGCCCCTCTACAAGCAGGTGCTCTCCGACCGTGAGAGCTTGA
                     GCTTCTACGGCGAGGGCTACACCTCCGACGAGGAGGTGCTGGAGGTCTTCCGCAACAC
                     CCTCAACAAGAACTCAGAGATCTTCTCCTCCATCAAGAAGCTGGAGAAGCTCTTCAAG
                     AACTTCGACGAGTACTCCTCCGCCGGCATCTTCGTGAAGAACGGCCCGGCCATCTCAA
                     CCATCTCCAAGGACATCTTCGGCGAGTGGAACGTCATCCGTGACAAGTGGAACGCCGA
                     GTACGACGACATCCACCTCAAGAAGAAGGCCGTGGTGACCGAGAAGTACGAGGACGAC
                     CGCCGCAAGAGCTTCAAGAAGATCGGCAGCTTCTCACTGGAGCAGCTCCAGGAGTACG
                     CCGACGCCGACCTCTCCGTGGTGGAGAAGCTGAAGGAGATCATCATCCAGAAGGTGGA
                     CGAGATCTACAAGGTGTACGGCAGCTCTGAGAAGCTCTTCGACGCCGACTTCGTCCTG
                     GAGAAGAGCCTCAAGAAGAACGACGCCGTGGTGGCCATCATGAAGGACCTCCTCGACT
                     CAGTCAAGAGCTTCGAGAACTACATCAAGGCCTTCTTCGGCGAGGGCAAGGAGACAAA
                     CCGCGACGAGAGCTTCTACGGCGACTTCGTCCTCGCCTATGACATCCTCCTCAAGGTG
                     GACCACATCTACGACGCCATCAGGAACTACGTGACCCAGAAGCCCTACTCAAAGGACA
                     AGTTCAAGCTCTACTTCCAGAACCCCCAGTTCATGGGCGGCTGGGACAAGGACAAGGA
                     GACTGACTACCGCGCCACCATCCTCCGCTACGGCTCAAAGTACTACCTCGCCATCATG
                     GACAAGAAGTACGCCAAGTGCCTCCAGAAGATCGACAAGGATGATGTGAACGGCAACT
                     ACGAGAAGATCAACTACAAGCTCCTCCCCGGCCCCAACAAGATGCTACCCAAGGTCTT
                     CTTCTCCAAGAAGTGGATGGCCTACTACAACCCCTCCGAGGACATCCAGAAGATCTAC
                     AAGAACGGCACCTTCAAGAAGGGTGACATGTTCAACCTCAACGACTGCCACAAGCTGA
                     TCGACTTCTTCAAGGACAGCATCTCAAGGTACCCCAAGTGGAGCAACGCCTACGACTT
                     CAACTTCTCTGAGACGGAGAAGTACAAGGACATCGCCGGCTTCTACCGGGAGGTGGAG
                     GAGCAAGGCTACAAGGTGAGCTTCGAGTCCGCCTCCAAGAAGGAGGTGGACAAGCTGG
                     TGGAGGAGGGCAAGCTCTACATGTTCCAGATCTACAACAAGGACTTCTCTGACAAGAG
                     CCACGGCACCCCCAACCTACACACCATGTACTTCAAGCTCCTCTTCGACGAGAACAAC
                     CACGGCCAGATCCGCCTCTCCGGCGGCGCCGAGCTCTTCATGAGGAGGGCCTCCCTCA
                     AGAAGGAGGAGCTGGTGGTTCACCCGGCCAACAGCCCCATCGCCAACAAGAACCCTGA
                     CAACCCCAAGAAGACCACCACCCTCTCCTACGACGTCTACAAGGACAAGAGGTTCTCA
                     GAAGACCAGTACGAGCTCCACATCCCCATCGCCATCAACAAGTGCCCCAAGAACATCT
                     TCAAGATCAACACCGAGGTGAGGGTGCTACTCAAGCATGATGATAACCCCTACGTCAT
                     CGGCATCGCCCGCGGCGAGAGGAACCTCCTCTACATCGTGGTGGTGGACGGCAAGGGC
                     AACATCGTGGAGCAGTACTCCTTGAACGAGATCATCAACAACTTCAACGGCATCAGGA
                     TCAAGACCGACTACCACTCACTCCTTGACAAGAAGGAGAAGGAGAGGTTCGAGGCCCG
                     GCAAAATTGGACAAGCATCGAGAACATCAAGGAGCTGAAGGCCGGCTACATCTCCCAG
                     GTGGTGCACAAGATCTGTGAGCTGGTGGAGAAGTACGACGCCGTCATCGCCCTGGAGG
                     ACCTCAACAGCGGCTTCAAGAACTCAAGGGTCAAGGTGGAGAAGCAGGTGTACCAGAA
                     GTTCGAGAAGATGCTCATCGACAAGCTGAACTACATGGTGGACAAGAAGAGCAACCCC
                     TGCGCCACCGGCGGCGCCCTCAAGGGCTACCAGATCACCAACAAGTTTGAGAGCTTCA
                     AGAGCATGTCAACCCAGAACGGCTTCATCTTCTACATCCCGGCCTGGCTCACCTCCAA
                     GATCGACCCCTCCACCGGCTTCGTCAACCTCCTCAAGACCAAGTACACCTCCATAGCC
                     GACAGCAAGAAGTTCATCTCCTCCTTCGACCGCATCATGTACGTCCCTGAGGAGGACC
                     TCTTCGAGTTCGCCCTCGACTACAAGAACTTCTCAAGGACCGACGCCGACTACATCAA
                     GAAGTGGAAGCTCTACAGCTACGGCAACCGCATCAGGATCTTCAGGAACCCCAAGAAG
                     AACAACGTCTTCGACTGGGAGGAGGTGTGCCTGACCTCCGCCTACAAGGAGCTCTTCA
                     ACAAGTACGGCATCAACTACCAGCAAGGTGACATCAGGGCGCTCCTATGCGAGCAGAG
                     CGACAAGGCCTTCTACTCCTCCTTCATGGCCCTCATGAGCTTGATGCTCCAGATGAGG
                     AACAGCATCACCGGCCGCACCGACGTCGACTTCCTCATCTCCCCCGTCAAGAACTCTG
                     ATGGCATCTTCTACGACTCAAGGAACTACGAGGCCCAGGAGAACGCCATCCTCCCCAA
                     GAACGCCGACGCCAACGGCGCCTACAACATCGCCAGGAAGGTGCTCTGGGCCATCGGC
                     CAGTTCAAGAAGGCCGAGGATGAGAAGCTGGACAAGGTGAAGATCGCCATCTCAAACA
                     AGGAGTGGCTGGAGTACGCCCAGACCTCCGTCAAGCAC"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TaLbCas12a(D156R-D832A)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:qc-ecppbux"">TaLbCas12a(D156R-D832A
                     )</a>"
                     /label="TaLbCas12a(D156R-D832A)"
     CDS             6638..6988
                     /Name="Synthetic coding sequence of the cytidine deaminase
                     base editor, 3rd generation gene   (Zong Y. et al., 2017)"
                     /Clone_Manager_'Draw_As'="Gene"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="nucNLS-UGI-SV40"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:6l-ecue642"">nucNLS-UGI-SV40</a>"
                     /label="nucNLS-UGI-SV40"
     CDS             6989..7045
                     /created_by="User"
                     /Transferred_From="P2A"
                     /Transferred_Similarity="100.00%"
                     /label="P2A"
     CDS             7046..7762
                     /note="A206K"
                     /codon_start=1
                     /transl_table=11
                     /product="GFP"
                     /protein_id="AHE38511.1"
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="GFP"
                     /Transferred_Similarity="100.00%"
                     /label="GFP"
     Linker          7766..7813
                     /created_by="wacae"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             7814..8062
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      8070..8341
                     /label="G7T"
     Linker          8346..8394
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(8431..8451)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        8512..8562
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    8563..9372
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      9519..10107
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          10706..10726
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta
       61 taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt
      121 atacatatat ttaaacttta ctctacgaat aatataatct atagtactac aataatatca
      181 gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt
      241 ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg
      301 caaatagctt cacctatata atacttcatc cattttatta gtacatccat ttagggttta
      361 gggttaatgg tttttataga ctaatttttt tagtacatct attttattct attttagcct
      421 ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa
      481 tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta
      541 aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt
      601 ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca
      661 cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg
      721 ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag
      781 gcggcctcct cctcctctca cggcacggca gctacggggg attcctttcc caccgctcct
      841 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
      901 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
      961 ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc taccttctct
     1021 agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc atgtttgtgt
     1081 tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac
     1141 gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc
     1201 tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt
     1261 tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt
     1321 tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc
     1381 ggagtagaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg
     1441 tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata
     1501 ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg
     1561 gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac
     1621 tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct
     1681 tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat
     1741 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     1801 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     1861 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     1921 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     1981 tctgcaaaca atgaaaagaa ccgccgacgg cagcgaattc gagcctaaga agaagaggaa
     2041 ggtttccggc ggcagctcag gaggttccga agcctctcct gcctctggac ccagacacct
     2101 gatggaccct cacatcttca ccagcaactt caacaacggc atcggccggc acaagaccta
     2161 cctgtgctac gaggtggaac ggctggacaa tggcaccagc gtgaagatgg accagcaccg
     2221 gggctttctg cacaaccagg ccaagaatct gctgtgcggc ttctacggca gacacgccga
     2281 gctgagattt ctggacctgg tgcctagcct gcagctggac cctgctcaga tctacagagt
     2341 gacctggttc atcaggtaag tttctgcttc tacctttgat atatatataa taattatcat
     2401 taattagtag taatataata tttcaaatat ttttttcaaa ataaaagaat gtagtatata
     2461 gcaattgctt ttctgtagtt tataagtgtg tatattttaa tttataactt ttctaatata
     2521 tgaccaaaat ttgttgatgt gcagctggtc cccatgcttc tcttggggct gtgctggcga
     2581 agtgcgcgcc ttcctgcaag agaatactca cgtgcggctg cggatcttcg ccgccagaat
     2641 ctacgactac gaccctctgt acaaagaggc cctgcagatg ctgagagatg ccggtgctca
     2701 ggtgtccatc atgacctacg acgagttcaa gcactgctgg gacaccttcg tggatcacca
     2761 gggctgccct ttccagcctt gggatggact ggatgagcac tctcaggccc tgagcggtag
     2821 actgagagcc atcctgcaga accagggcaa cggtggcgga ggttctggcg gtggaggatc
     2881 cggaggtggc gggtccggtg gtggcggatc aggtggagga ggttcgggag gcggaggtag
     2941 tggctccatg agcaagctgg agaagttcac caactgctac tcactctcca agaccctccg
     3001 cttcaaggcc atccccgtcg gcaagaccca ggagaacatc gacaacaaga ggctgctggt
     3061 ggaggatgag aagagggccg aggactacaa gggcgtcaag aagctcctcg accggtacta
     3121 cctctcattc atcaatgatg tcctccactc catcaagctg aagaacctca acaactacat
     3181 ctccctcttc cgcaagaaga caaggaccga gaaggagaac aaggagctgg agaacctgga
     3241 gatcaacctc cgcaaggaga tcgccaaggc cttcaagggc aacgagggct acaagagcct
     3301 cttcaagaag gacatcatcg agacgatcct accggagttc ctggatgaca aggacgagat
     3361 cgccctggtg aacagcttca acggcttcac caccgccttc accggcttct tccgcaaccg
     3421 ggagaacatg ttctccgagg aggccaagag cacctccatc gccttcaggt gcatcaacga
     3481 gaacctcacc cgctacatct caaacatgga catcttcgag aaggtggacg ccatcttcga
     3541 caagcatgag gtccaggaga tcaaggagaa gatcctgaac tccgactacg acgtggagga
     3601 cttcttcgag ggcgagttct tcaacttcgt cctgacccaa gaaggcatcg acgtctacaa
     3661 cgccatcatc ggcggcttcg tcaccgagtc aggtgagaag atcaagggcc tcaacgagta
     3721 catcaacctc tacaaccaga agaccaagca gaagctcccc aagttcaagc ccctctacaa
     3781 gcaggtgctc tccgaccgtg agagcttgag cttctacggc gagggctaca cctccgacga
     3841 ggaggtgctg gaggtcttcc gcaacaccct caacaagaac tcagagatct tctcctccat
     3901 caagaagctg gagaagctct tcaagaactt cgacgagtac tcctccgccg gcatcttcgt
     3961 gaagaacggc ccggccatct caaccatctc caaggacatc ttcggcgagt ggaacgtcat
     4021 ccgtgacaag tggaacgccg agtacgacga catccacctc aagaagaagg ccgtggtgac
     4081 cgagaagtac gaggacgacc gccgcaagag cttcaagaag atcggcagct tctcactgga
     4141 gcagctccag gagtacgccg acgccgacct ctccgtggtg gagaagctga aggagatcat
     4201 catccagaag gtggacgaga tctacaaggt gtacggcagc tctgagaagc tcttcgacgc
     4261 cgacttcgtc ctggagaaga gcctcaagaa gaacgacgcc gtggtggcca tcatgaagga
     4321 cctcctcgac tcagtcaaga gcttcgagaa ctacatcaag gccttcttcg gcgagggcaa
     4381 ggagacaaac cgcgacgaga gcttctacgg cgacttcgtc ctcgcctatg acatcctcct
     4441 caaggtggac cacatctacg acgccatcag gaactacgtg acccagaagc cctactcaaa
     4501 ggacaagttc aagctctact tccagaaccc ccagttcatg ggcggctggg acaaggacaa
     4561 ggagactgac taccgcgcca ccatcctccg ctacggctca aagtactacc tcgccatcat
     4621 ggacaagaag tacgccaagt gcctccagaa gatcgacaag gatgatgtga acggcaacta
     4681 cgagaagatc aactacaagc tcctccccgg ccccaacaag atgctaccca aggtcttctt
     4741 ctccaagaag tggatggcct actacaaccc ctccgaggac atccagaaga tctacaagaa
     4801 cggcaccttc aagaagggtg acatgttcaa cctcaacgac tgccacaagc tgatcgactt
     4861 cttcaaggac agcatctcaa ggtaccccaa gtggagcaac gcctacgact tcaacttctc
     4921 tgagacggag aagtacaagg acatcgccgg cttctaccgg gaggtggagg agcaaggcta
     4981 caaggtgagc ttcgagtccg cctccaagaa ggaggtggac aagctggtgg aggagggcaa
     5041 gctctacatg ttccagatct acaacaagga cttctctgac aagagccacg gcacccccaa
     5101 cctacacacc atgtacttca agctcctctt cgacgagaac aaccacggcc agatccgcct
     5161 ctccggcggc gccgagctct tcatgaggag ggcctccctc aagaaggagg agctggtggt
     5221 tcacccggcc aacagcccca tcgccaacaa gaaccctgac aaccccaaga agaccaccac
     5281 cctctcctac gacgtctaca aggacaagag gttctcagaa gaccagtacg agctccacat
     5341 ccccatcgcc atcaacaagt gccccaagaa catcttcaag atcaacaccg aggtgagggt
     5401 gctactcaag catgatgata acccctacgt catcggcatc gcccgcggcg agaggaacct
     5461 cctctacatc gtggtggtgg acggcaaggg caacatcgtg gagcagtact ccttgaacga
     5521 gatcatcaac aacttcaacg gcatcaggat caagaccgac taccactcac tccttgacaa
     5581 gaaggagaag gagaggttcg aggcccggca aaattggaca agcatcgaga acatcaagga
     5641 gctgaaggcc ggctacatct cccaggtggt gcacaagatc tgtgagctgg tggagaagta
     5701 cgacgccgtc atcgccctgg aggacctcaa cagcggcttc aagaactcaa gggtcaaggt
     5761 ggagaagcag gtgtaccaga agttcgagaa gatgctcatc gacaagctga actacatggt
     5821 ggacaagaag agcaacccct gcgccaccgg cggcgccctc aagggctacc agatcaccaa
     5881 caagtttgag agcttcaaga gcatgtcaac ccagaacggc ttcatcttct acatcccggc
     5941 ctggctcacc tccaagatcg acccctccac cggcttcgtc aacctcctca agaccaagta
     6001 cacctccata gccgacagca agaagttcat ctcctccttc gaccgcatca tgtacgtccc
     6061 tgaggaggac ctcttcgagt tcgccctcga ctacaagaac ttctcaagga ccgacgccga
     6121 ctacatcaag aagtggaagc tctacagcta cggcaaccgc atcaggatct tcaggaaccc
     6181 caagaagaac aacgtcttcg actgggagga ggtgtgcctg acctccgcct acaaggagct
     6241 cttcaacaag tacggcatca actaccagca aggtgacatc agggcgctcc tatgcgagca
     6301 gagcgacaag gccttctact cctccttcat ggccctcatg agcttgatgc tccagatgag
     6361 gaacagcatc accggccgca ccgacgtcga cttcctcatc tcccccgtca agaactctga
     6421 tggcatcttc tacgactcaa ggaactacga ggcccaggag aacgccatcc tccccaagaa
     6481 cgccgacgcc aacggcgcct acaacatcgc caggaaggtg ctctgggcca tcggccagtt
     6541 caagaaggcc gaggatgaga agctggacaa ggtgaagatc gccatctcaa acaaggagtg
     6601 gctggagtac gcccagacct ccgtcaagca ctcaggtaag aggccggcgg ccaccaagaa
     6661 ggccggccaa gccaagaaga agaagacaag agattcagga ggatccacca acctctccga
     6721 catcatcgag aaggagactg gcaagcagct ggtgatccag gagagcatct tgatgctgcc
     6781 tgaggaggtg gaggaggtca tcggcaacaa gccggagtcc gacatcctcg tccacaccgc
     6841 ctacgacgag tccaccgacg agaacgtcat gctcctcacc tcagatgctc cagagtacaa
     6901 gccctgggcc ttggtgatcc aagactccaa cggcgagaac aagatcaaga tgctctccgg
     6961 cggcagcccc aagaagaaga ggaaggttgc tactaatttt tctcttctta agcaagctgg
     7021 agatgttgaa gaaaatcctg gacccatggt gagcaagggc gaggagctgt tcaccggggt
     7081 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     7141 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     7201 caagctgccc gtgccctggc ccaccctcgt gaccaccttc acctacggcg tgcagtgctt
     7261 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     7321 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     7381 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     7441 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     7501 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     7561 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     7621 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     7681 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactca
     7741 cggcatggac gagctgtaca agtcaggttc tggttcaggt tcaggttctg gatctggaag
     7801 tggatctgga tcagcggccg catctgaatt caagcgtgaa gagcaagcaa ggaaagctaa
     7861 ggtgaacaat gagaaaaaga cggaaatagt gaaaccagag agttgtagca atgaaggaga
     7921 tgtcaaggat ctgaaaagaa aggactctga ggatggaaac gagggtgagg aagaagaagc
     7981 ttcttcgaaa ccgaaaaagc caaaagttgc tctttctcat cttcaggaca ttgacgacac
     8041 agaagctgat caagaagaag agtaactgcc cccgatgagc taagctagct atatcatcaa
     8101 tttatgtatt acacataata tcgcactcag tctttcatct acggcaatgt accagctgat
     8161 ataatcagtt attgaaatat ttctgaattt aaacttgcat caataaattt atgtttttgc
     8221 ttggactata atacctgact tgttatttta tcaataaata tttaaactat atttctttca
     8281 agatgggaat taacatctac aaattgcctt ttcttatcga ccatgtactc gacggccgag
     8341 tactataagc taggtagtaa ctagtgcgac tgtctagaat cgcgtatgcg agtgtatgag
     8401 ctcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg
     8461 cccgtgtctc aaaatctctg atgttacatt gcacaagata aaataatatc atcatgaaca
     8521 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
     8581 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     8641 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     8701 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     8761 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     8821 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag
     8881 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     8941 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt
     9001 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     9061 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     9121 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     9181 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     9241 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     9301 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     9361 gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact
     9421 tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac
     9481 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9541 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9601 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9661 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9721 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9781 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9841 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9901 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9961 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    10021 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10081 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10141 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    10201 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    10261 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    10321 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    10381 gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa
    10441 ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc
    10501 ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg
    10561 tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc cagacttccg
    10621 actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca
    10681 tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggcccacc
    10741 t
//
