LOCUS       pZmUBI-BP-TadA9-6xGGGGS-TaLbCas12a(D156R-D832A)-BP-P2A-GFP-N7-G7T       10156 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...d1-en4mbdp
VERSION     urn.local...d1-en4mbdp
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1986
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/1;
                     ZmUBIL.2 (<a
                     href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     intron          978..1987
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     NLS             1994..2044
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /modified_by="mamee"
                     /label="BP*"
     CDS             2045..2068
                     /created_by="chgai"
                     /label="2xSGGS"
     CDS             2069..2566
                     /standard_name="TadAmax10 (ABE8)"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TadA9"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:bn-ecuitju"">TadA9</a>"
                     /label="TadA9"
     Linker          2567..2656
                     /modified_by="mamee"
                     /label="linker"
     CDS             2663..6346
                     /%_Identity=100
                     /Motif="ATGAGCAAGCTGGAGAAGTTCACCAACTGCTACTCACTCTCCAAGACCCT
                     CCGCTTCAAGGCCATCCCCGTCGGCAAGACCCAGGAGAACATCGACAACAAGAGGCTG
                     CTGGTGGAGGATGAGAAGAGGGCCGAGGACTACAAGGGCGTCAAGAAGCTCCTCGACC
                     GGTACTACCTCTCATTCATCAATGATGTCCTCCACTCCATCAAGCTGAAGAACCTCAA
                     CAACTACATCTCCCTCTTCCGCAAGAAGACAAGGACCGAGAAGGAGAACAAGGAGCTG
                     GAGAACCTGGAGATCAACCTCCGCAAGGAGATCGCCAAGGCCTTCAAGGGCAACGAGG
                     GCTACAAGAGCCTCTTCAAGAAGGACATCATCGAGACGATCCTACCGGAGTTCCTGGA
                     TGACAAGGACGAGATCGCCCTGGTGAACAGCTTCAACGGCTTCACCACCGCCTTCACC
                     GGCTTCTTCCGCAACCGGGAGAACATGTTCTCCGAGGAGGCCAAGAGCACCTCCATCG
                     CCTTCAGGTGCATCAACGAGAACCTCACCCGCTACATCTCAAACATGGACATCTTCGA
                     GAAGGTGGACGCCATCTTCGACAAGCATGAGGTCCAGGAGATCAAGGAGAAGATCCTG
                     AACTCCGACTACGACGTGGAGGACTTCTTCGAGGGCGAGTTCTTCAACTTCGTCCTGA
                     CCCAAGAAGGCATCGACGTCTACAACGCCATCATCGGCGGCTTCGTCACCGAGTCAGG
                     TGAGAAGATCAAGGGCCTCAACGAGTACATCAACCTCTACAACCAGAAGACCAAGCAG
                     AAGCTCCCCAAGTTCAAGCCCCTCTACAAGCAGGTGCTCTCCGACCGTGAGAGCTTGA
                     GCTTCTACGGCGAGGGCTACACCTCCGACGAGGAGGTGCTGGAGGTCTTCCGCAACAC
                     CCTCAACAAGAACTCAGAGATCTTCTCCTCCATCAAGAAGCTGGAGAAGCTCTTCAAG
                     AACTTCGACGAGTACTCCTCCGCCGGCATCTTCGTGAAGAACGGCCCGGCCATCTCAA
                     CCATCTCCAAGGACATCTTCGGCGAGTGGAACGTCATCCGTGACAAGTGGAACGCCGA
                     GTACGACGACATCCACCTCAAGAAGAAGGCCGTGGTGACCGAGAAGTACGAGGACGAC
                     CGCCGCAAGAGCTTCAAGAAGATCGGCAGCTTCTCACTGGAGCAGCTCCAGGAGTACG
                     CCGACGCCGACCTCTCCGTGGTGGAGAAGCTGAAGGAGATCATCATCCAGAAGGTGGA
                     CGAGATCTACAAGGTGTACGGCAGCTCTGAGAAGCTCTTCGACGCCGACTTCGTCCTG
                     GAGAAGAGCCTCAAGAAGAACGACGCCGTGGTGGCCATCATGAAGGACCTCCTCGACT
                     CAGTCAAGAGCTTCGAGAACTACATCAAGGCCTTCTTCGGCGAGGGCAAGGAGACAAA
                     CCGCGACGAGAGCTTCTACGGCGACTTCGTCCTCGCCTATGACATCCTCCTCAAGGTG
                     GACCACATCTACGACGCCATCAGGAACTACGTGACCCAGAAGCCCTACTCAAAGGACA
                     AGTTCAAGCTCTACTTCCAGAACCCCCAGTTCATGGGCGGCTGGGACAAGGACAAGGA
                     GACTGACTACCGCGCCACCATCCTCCGCTACGGCTCAAAGTACTACCTCGCCATCATG
                     GACAAGAAGTACGCCAAGTGCCTCCAGAAGATCGACAAGGATGATGTGAACGGCAACT
                     ACGAGAAGATCAACTACAAGCTCCTCCCCGGCCCCAACAAGATGCTACCCAAGGTCTT
                     CTTCTCCAAGAAGTGGATGGCCTACTACAACCCCTCCGAGGACATCCAGAAGATCTAC
                     AAGAACGGCACCTTCAAGAAGGGTGACATGTTCAACCTCAACGACTGCCACAAGCTGA
                     TCGACTTCTTCAAGGACAGCATCTCAAGGTACCCCAAGTGGAGCAACGCCTACGACTT
                     CAACTTCTCTGAGACGGAGAAGTACAAGGACATCGCCGGCTTCTACCGGGAGGTGGAG
                     GAGCAAGGCTACAAGGTGAGCTTCGAGTCCGCCTCCAAGAAGGAGGTGGACAAGCTGG
                     TGGAGGAGGGCAAGCTCTACATGTTCCAGATCTACAACAAGGACTTCTCTGACAAGAG
                     CCACGGCACCCCCAACCTACACACCATGTACTTCAAGCTCCTCTTCGACGAGAACAAC
                     CACGGCCAGATCCGCCTCTCCGGCGGCGCCGAGCTCTTCATGAGGAGGGCCTCCCTCA
                     AGAAGGAGGAGCTGGTGGTTCACCCGGCCAACAGCCCCATCGCCAACAAGAACCCTGA
                     CAACCCCAAGAAGACCACCACCCTCTCCTACGACGTCTACAAGGACAAGAGGTTCTCA
                     GAAGACCAGTACGAGCTCCACATCCCCATCGCCATCAACAAGTGCCCCAAGAACATCT
                     TCAAGATCAACACCGAGGTGAGGGTGCTACTCAAGCATGATGATAACCCCTACGTCAT
                     CGGCATCGCCCGCGGCGAGAGGAACCTCCTCTACATCGTGGTGGTGGACGGCAAGGGC
                     AACATCGTGGAGCAGTACTCCTTGAACGAGATCATCAACAACTTCAACGGCATCAGGA
                     TCAAGACCGACTACCACTCACTCCTTGACAAGAAGGAGAAGGAGAGGTTCGAGGCCCG
                     GCAAAATTGGACAAGCATCGAGAACATCAAGGAGCTGAAGGCCGGCTACATCTCCCAG
                     GTGGTGCACAAGATCTGTGAGCTGGTGGAGAAGTACGACGCCGTCATCGCCCTGGAGG
                     ACCTCAACAGCGGCTTCAAGAACTCAAGGGTCAAGGTGGAGAAGCAGGTGTACCAGAA
                     GTTCGAGAAGATGCTCATCGACAAGCTGAACTACATGGTGGACAAGAAGAGCAACCCC
                     TGCGCCACCGGCGGCGCCCTCAAGGGCTACCAGATCACCAACAAGTTTGAGAGCTTCA
                     AGAGCATGTCAACCCAGAACGGCTTCATCTTCTACATCCCGGCCTGGCTCACCTCCAA
                     GATCGACCCCTCCACCGGCTTCGTCAACCTCCTCAAGACCAAGTACACCTCCATAGCC
                     GACAGCAAGAAGTTCATCTCCTCCTTCGACCGCATCATGTACGTCCCTGAGGAGGACC
                     TCTTCGAGTTCGCCCTCGACTACAAGAACTTCTCAAGGACCGACGCCGACTACATCAA
                     GAAGTGGAAGCTCTACAGCTACGGCAACCGCATCAGGATCTTCAGGAACCCCAAGAAG
                     AACAACGTCTTCGACTGGGAGGAGGTGTGCCTGACCTCCGCCTACAAGGAGCTCTTCA
                     ACAAGTACGGCATCAACTACCAGCAAGGTGACATCAGGGCGCTCCTATGCGAGCAGAG
                     CGACAAGGCCTTCTACTCCTCCTTCATGGCCCTCATGAGCTTGATGCTCCAGATGAGG
                     AACAGCATCACCGGCCGCACCGACGTCGACTTCCTCATCTCCCCCGTCAAGAACTCTG
                     ATGGCATCTTCTACGACTCAAGGAACTACGAGGCCCAGGAGAACGCCATCCTCCCCAA
                     GAACGCCGACGCCAACGGCGCCTACAACATCGCCAGGAAGGTGCTCTGGGCCATCGGC
                     CAGTTCAAGAAGGCCGAGGATGAGAAGCTGGACAAGGTGAAGATCGCCATCTCAAACA
                     AGGAGTGGCTGGAGTACGCCCAGACCTCCGTCAAGCAC"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TaLbCas12a(D156R-D832A)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:qc-ecppbux"">TaLbCas12a(D156R-D832A
                     )</a>"
                     /label="TaLbCas12a(D156R-D832A)"
     NLS             6353..6403
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     CDS             6404..6460
                     /created_by="User"
                     /Transferred_From="P2A"
                     /Transferred_Similarity="100.00%"
                     /label="P2A"
     CDS             6461..7177
                     /note="A206K"
                     /codon_start=1
                     /transl_table=11
                     /product="GFP"
                     /protein_id="AHE38511.1"
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="GFP"
                     /Transferred_Similarity="100.00%"
                     /label="GFP"
     Linker          7181..7228
                     /created_by="wacae"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             7229..7477
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      7485..7756
                     /label="G7T"
     Linker          7761..7809
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(7846..7866)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        7927..7977
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    7978..8787
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      8934..9522
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          10121..10141
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta
       61 taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt
      121 atacatatat ttaaacttta ctctacgaat aatataatct atagtactac aataatatca
      181 gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt
      241 ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg
      301 caaatagctt cacctatata atacttcatc cattttatta gtacatccat ttagggttta
      361 gggttaatgg tttttataga ctaatttttt tagtacatct attttattct attttagcct
      421 ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa
      481 tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta
      541 aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt
      601 ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca
      661 cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg
      721 ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag
      781 gcggcctcct cctcctctca cggcacggca gctacggggg attcctttcc caccgctcct
      841 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
      901 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
      961 ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc taccttctct
     1021 agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc atgtttgtgt
     1081 tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac
     1141 gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc
     1201 tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt
     1261 tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt
     1321 tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc
     1381 ggagtagaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg
     1441 tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata
     1501 ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg
     1561 gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac
     1621 tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct
     1681 tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat
     1741 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     1801 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     1861 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     1921 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     1981 tctgcaaaca atgaaaagaa ccgccgacgg cagcgaattc gagcctaaga agaagaggaa
     2041 ggtttccggc ggcagctcag gaggttcctc tgaggtggag ttttcccacg agtactggat
     2101 gagacatgcc ctgaccctgg ccaagagggc acgggatgag agggaggtgc ctgtgggagc
     2161 cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg aacagagcca tcggcctgca
     2221 cgacccaaca gcccatgccg aaattatggc cctgagacag ggcggcctgg tcatgcagaa
     2281 ctacagactg attgacgcca ccctgtactc gacattcgag ccttgcgtga tgtgcgccgg
     2341 cgccatgatc cactctagga tcggccgcgt ggtgtttggc gtgaggaact caaaaagagg
     2401 cgccgcaggc tccctgatga acgtgctgaa ctaccccggc atgaatcacc gcgtcgaaat
     2461 taccgaggga atcctggcag atgaatgtgc cgccctgctg tgcgatttct atcggatgcc
     2521 tagacgggtg ttcaatgctc agaagaaggc ccagagctcc atcaacggtg gcggaggttc
     2581 tggcggtgga ggatccggag gtggcgggtc cggtggtggc ggatcaggtg gaggaggttc
     2641 gggaggcgga ggtagtggct ccatgagcaa gctggagaag ttcaccaact gctactcact
     2701 ctccaagacc ctccgcttca aggccatccc cgtcggcaag acccaggaga acatcgacaa
     2761 caagaggctg ctggtggagg atgagaagag ggccgaggac tacaagggcg tcaagaagct
     2821 cctcgaccgg tactacctct cattcatcaa tgatgtcctc cactccatca agctgaagaa
     2881 cctcaacaac tacatctccc tcttccgcaa gaagacaagg accgagaagg agaacaagga
     2941 gctggagaac ctggagatca acctccgcaa ggagatcgcc aaggccttca agggcaacga
     3001 gggctacaag agcctcttca agaaggacat catcgagacg atcctaccgg agttcctgga
     3061 tgacaaggac gagatcgccc tggtgaacag cttcaacggc ttcaccaccg ccttcaccgg
     3121 cttcttccgc aaccgggaga acatgttctc cgaggaggcc aagagcacct ccatcgcctt
     3181 caggtgcatc aacgagaacc tcacccgcta catctcaaac atggacatct tcgagaaggt
     3241 ggacgccatc ttcgacaagc atgaggtcca ggagatcaag gagaagatcc tgaactccga
     3301 ctacgacgtg gaggacttct tcgagggcga gttcttcaac ttcgtcctga cccaagaagg
     3361 catcgacgtc tacaacgcca tcatcggcgg cttcgtcacc gagtcaggtg agaagatcaa
     3421 gggcctcaac gagtacatca acctctacaa ccagaagacc aagcagaagc tccccaagtt
     3481 caagcccctc tacaagcagg tgctctccga ccgtgagagc ttgagcttct acggcgaggg
     3541 ctacacctcc gacgaggagg tgctggaggt cttccgcaac accctcaaca agaactcaga
     3601 gatcttctcc tccatcaaga agctggagaa gctcttcaag aacttcgacg agtactcctc
     3661 cgccggcatc ttcgtgaaga acggcccggc catctcaacc atctccaagg acatcttcgg
     3721 cgagtggaac gtcatccgtg acaagtggaa cgccgagtac gacgacatcc acctcaagaa
     3781 gaaggccgtg gtgaccgaga agtacgagga cgaccgccgc aagagcttca agaagatcgg
     3841 cagcttctca ctggagcagc tccaggagta cgccgacgcc gacctctccg tggtggagaa
     3901 gctgaaggag atcatcatcc agaaggtgga cgagatctac aaggtgtacg gcagctctga
     3961 gaagctcttc gacgccgact tcgtcctgga gaagagcctc aagaagaacg acgccgtggt
     4021 ggccatcatg aaggacctcc tcgactcagt caagagcttc gagaactaca tcaaggcctt
     4081 cttcggcgag ggcaaggaga caaaccgcga cgagagcttc tacggcgact tcgtcctcgc
     4141 ctatgacatc ctcctcaagg tggaccacat ctacgacgcc atcaggaact acgtgaccca
     4201 gaagccctac tcaaaggaca agttcaagct ctacttccag aacccccagt tcatgggcgg
     4261 ctgggacaag gacaaggaga ctgactaccg cgccaccatc ctccgctacg gctcaaagta
     4321 ctacctcgcc atcatggaca agaagtacgc caagtgcctc cagaagatcg acaaggatga
     4381 tgtgaacggc aactacgaga agatcaacta caagctcctc cccggcccca acaagatgct
     4441 acccaaggtc ttcttctcca agaagtggat ggcctactac aacccctccg aggacatcca
     4501 gaagatctac aagaacggca ccttcaagaa gggtgacatg ttcaacctca acgactgcca
     4561 caagctgatc gacttcttca aggacagcat ctcaaggtac cccaagtgga gcaacgccta
     4621 cgacttcaac ttctctgaga cggagaagta caaggacatc gccggcttct accgggaggt
     4681 ggaggagcaa ggctacaagg tgagcttcga gtccgcctcc aagaaggagg tggacaagct
     4741 ggtggaggag ggcaagctct acatgttcca gatctacaac aaggacttct ctgacaagag
     4801 ccacggcacc cccaacctac acaccatgta cttcaagctc ctcttcgacg agaacaacca
     4861 cggccagatc cgcctctccg gcggcgccga gctcttcatg aggagggcct ccctcaagaa
     4921 ggaggagctg gtggttcacc cggccaacag ccccatcgcc aacaagaacc ctgacaaccc
     4981 caagaagacc accaccctct cctacgacgt ctacaaggac aagaggttct cagaagacca
     5041 gtacgagctc cacatcccca tcgccatcaa caagtgcccc aagaacatct tcaagatcaa
     5101 caccgaggtg agggtgctac tcaagcatga tgataacccc tacgtcatcg gcatcgcccg
     5161 cggcgagagg aacctcctct acatcgtggt ggtggacggc aagggcaaca tcgtggagca
     5221 gtactccttg aacgagatca tcaacaactt caacggcatc aggatcaaga ccgactacca
     5281 ctcactcctt gacaagaagg agaaggagag gttcgaggcc cggcaaaatt ggacaagcat
     5341 cgagaacatc aaggagctga aggccggcta catctcccag gtggtgcaca agatctgtga
     5401 gctggtggag aagtacgacg ccgtcatcgc cctggaggac ctcaacagcg gcttcaagaa
     5461 ctcaagggtc aaggtggaga agcaggtgta ccagaagttc gagaagatgc tcatcgacaa
     5521 gctgaactac atggtggaca agaagagcaa cccctgcgcc accggcggcg ccctcaaggg
     5581 ctaccagatc accaacaagt ttgagagctt caagagcatg tcaacccaga acggcttcat
     5641 cttctacatc ccggcctggc tcacctccaa gatcgacccc tccaccggct tcgtcaacct
     5701 cctcaagacc aagtacacct ccatagccga cagcaagaag ttcatctcct ccttcgaccg
     5761 catcatgtac gtccctgagg aggacctctt cgagttcgcc ctcgactaca agaacttctc
     5821 aaggaccgac gccgactaca tcaagaagtg gaagctctac agctacggca accgcatcag
     5881 gatcttcagg aaccccaaga agaacaacgt cttcgactgg gaggaggtgt gcctgacctc
     5941 cgcctacaag gagctcttca acaagtacgg catcaactac cagcaaggtg acatcagggc
     6001 gctcctatgc gagcagagcg acaaggcctt ctactcctcc ttcatggccc tcatgagctt
     6061 gatgctccag atgaggaaca gcatcaccgg ccgcaccgac gtcgacttcc tcatctcccc
     6121 cgtcaagaac tctgatggca tcttctacga ctcaaggaac tacgaggccc aggagaacgc
     6181 catcctcccc aagaacgccg acgccaacgg cgcctacaac atcgccagga aggtgctctg
     6241 ggccatcggc cagttcaaga aggccgagga tgagaagctg gacaaggtga agatcgccat
     6301 ctcaaacaag gagtggctgg agtacgccca gacctccgtc aagcactcag gtaaaagaac
     6361 cgccgacggc agcgaattcg agcctaagaa gaagaggaag gttgctacta atttttctct
     6421 tcttaagcaa gctggagatg ttgaagaaaa tcctggaccc atggtgagca agggcgagga
     6481 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     6541 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     6601 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta
     6661 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     6721 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     6781 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     6841 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     6901 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     6961 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     7021 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
     7081 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     7141 cgccgggatc actcacggca tggacgagct gtacaagtca ggttctggtt caggttcagg
     7201 ttctggatct ggaagtggat ctggatcagc ggccgcatct gaattcaagc gtgaagagca
     7261 agcaaggaaa gctaaggtga acaatgagaa aaagacggaa atagtgaaac cagagagttg
     7321 tagcaatgaa ggagatgtca aggatctgaa aagaaaggac tctgaggatg gaaacgaggg
     7381 tgaggaagaa gaagcttctt cgaaaccgaa aaagccaaaa gttgctcttt ctcatcttca
     7441 ggacattgac gacacagaag ctgatcaaga agaagagtaa ctgcccccga tgagctaagc
     7501 tagctatatc atcaatttat gtattacaca taatatcgca ctcagtcttt catctacggc
     7561 aatgtaccag ctgatataat cagttattga aatatttctg aatttaaact tgcatcaata
     7621 aatttatgtt tttgcttgga ctataatacc tgacttgtta ttttatcaat aaatatttaa
     7681 actatatttc tttcaagatg ggaattaaca tctacaaatt gccttttctt atcgaccatg
     7741 tactcgacgg ccgagtacta taagctaggt agtaactagt gcgactgtct agaatcgcgt
     7801 atgcgagtgt atgagctcga tatcccctat agtgagtcgt attacatggt catagctgtt
     7861 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaata
     7921 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     7981 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     8041 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     8101 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     8161 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     8221 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     8281 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     8341 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     8401 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     8461 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     8521 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     8581 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     8641 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     8701 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     8761 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     8821 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     8881 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8941 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9001 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9061 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     9121 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9181 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9241 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9301 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9361 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9421 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9481 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9541 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9601 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9661 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9721 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     9781 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     9841 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     9901 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     9961 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
    10021 aggcccagac ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
    10081 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
    10141 aagctcgggc ccacct
//
