LOCUS       pZmUBI-BP-A3A-intron-nCas9-nucNLS-UGI-SV40-NLS-P2A-GFP-N7-G7T       11119 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...cv-en4mbdm
VERSION     urn.local...cv-en4mbdm
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1986
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/1;
                     ZmUBIL.2 (<a
                     href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     intron          978..1987
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     NLS             1994..2044
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     CDS             join(2069..2355,2545..2851)
                     /codon_start=1
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A3Aintron"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; APOBEC3A (<a
                     href=""urn:local:thjac:t-ecoeihu"">A3Aintron</a>)"
                     /label="APOBEC3A"
     intron          2356..2544
                     /vntifkey=15
                     /modified_by="thjac"
                     /label="IV2 intron"
     Linker          2852..2899
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="32-aa linker"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; XTEN (<a
                     href=""urn:local:.:a3-dgycryz"">32-aa linker</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a5-dgycryz"">XTEN</a>"
                     /label="XTEN"
     CDS             2906..7009
                     /X-Transferred_From="Cas9PTA"
                     /X-Transferred_Similarity="99.98%"
                     /X-Primary_Match="99.98%; Adjusted-Interval-0:0/0; CAS9
                     (<a href=""urn:local:thjac:3c-c4nnd56"">Cas9PTA</a>)"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="nCAS9(D10A)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:pe-ecpo7bv"">nCAS9(D10A)</a>"
                     /label="nCAS9(D10A)"
     CDS             7016..7366
                     /Name="Synthetic coding sequence of the cytidine deaminase
                     base editor, 3rd generation gene   (Zong Y. et al., 2017)"
                     /Clone_Manager_'Draw_As'="Gene"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="nucNLS-UGI-SV40"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:6l-ecue642"">nucNLS-UGI-SV40</a>"
                     /label="nucNLS-UGI-SV40"
     CDS             7367..7423
                     /created_by="User"
                     /Transferred_From="P2A"
                     /Transferred_Similarity="100.00%"
                     /label="P2A"
     CDS             7424..8140
                     /note="A206K"
                     /codon_start=1
                     /transl_table=11
                     /product="GFP"
                     /protein_id="AHE38511.1"
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="GFP"
                     /Transferred_Similarity="100.00%"
                     /label="GFP"
     Linker          8144..8191
                     /created_by="wacae"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             8192..8440
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      8448..8719
                     /label="G7T"
     Linker          8724..8772
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(8809..8829)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        8890..8940
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    8941..9750
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      9897..10485
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          11084..11104
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta
       61 taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt
      121 atacatatat ttaaacttta ctctacgaat aatataatct atagtactac aataatatca
      181 gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt
      241 ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg
      301 caaatagctt cacctatata atacttcatc cattttatta gtacatccat ttagggttta
      361 gggttaatgg tttttataga ctaatttttt tagtacatct attttattct attttagcct
      421 ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa
      481 tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta
      541 aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt
      601 ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca
      661 cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg
      721 ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag
      781 gcggcctcct cctcctctca cggcacggca gctacggggg attcctttcc caccgctcct
      841 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
      901 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
      961 ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc taccttctct
     1021 agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc atgtttgtgt
     1081 tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac
     1141 gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc
     1201 tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt
     1261 tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt
     1321 tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc
     1381 ggagtagaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg
     1441 tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata
     1501 ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg
     1561 gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac
     1621 tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct
     1681 tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat
     1741 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     1801 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     1861 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     1921 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     1981 tctgcaaaca atgaaaagaa ccgccgacgg cagcgaattc gagcctaaga agaagaggaa
     2041 ggtttccggc ggcagctcag gaggttccga agcctctcct gcctctggac ccagacacct
     2101 gatggaccct cacatcttca ccagcaactt caacaacggc atcggccggc acaagaccta
     2161 cctgtgctac gaggtggaac ggctggacaa tggcaccagc gtgaagatgg accagcaccg
     2221 gggctttctg cacaaccagg ccaagaatct gctgtgcggc ttctacggca gacacgccga
     2281 gctgagattt ctggacctgg tgcctagcct gcagctggac cctgctcaga tctacagagt
     2341 gacctggttc atcaggtaag tttctgcttc tacctttgat atatatataa taattatcat
     2401 taattagtag taatataata tttcaaatat ttttttcaaa ataaaagaat gtagtatata
     2461 gcaattgctt ttctgtagtt tataagtgtg tatattttaa tttataactt ttctaatata
     2521 tgaccaaaat ttgttgatgt gcagctggtc cccatgcttc tcttggggct gtgctggcga
     2581 agtgcgcgcc ttcctgcaag agaatactca cgtgcggctg cggatcttcg ccgccagaat
     2641 ctacgactac gaccctctgt acaaagaggc cctgcagatg ctgagagatg ccggtgctca
     2701 ggtgtccatc atgacctacg acgagttcaa gcactgctgg gacaccttcg tggatcacca
     2761 gggctgccct ttccagcctt gggatggact ggatgagcac tctcaggccc tgagcggtag
     2821 actgagagcc atcctgcaga accagggcaa ctctggttct gaaactcctg gaacttcaga
     2881 atctgctact cctgaatccg gctcgatgga taagaagtac tctatcggac tcgctatcgg
     2941 aactaactct gtgggatggg ctgtgatcac cgatgagtac aaggtgccat ctaagaagtt
     3001 caaggttctc ggaaacaccg ataggcactc tatcaagaaa aaccttatcg gtgctctcct
     3061 cttcgattct ggtgaaactg ctgaggctac cagactcaag agaaccgcta gaagaaggta
     3121 caccagaaga aagaacagga tctgctacct ccaagagatc ttctctaacg agatggctaa
     3181 agtggatgat tcattcttcc acaggctcga agagtcattc ctcgtggaag aagataagaa
     3241 gcacgagagg caccctatct tcggaaacat cgttgatgag gtggcatacc acgagaagta
     3301 ccctactatc taccacctca gaaagaagct cgttgattct actgataagg ctgatctcag
     3361 gctcatctac ctcgctctcg ctcacatgat caagttcaga ggacacttcc tcatcgaggg
     3421 tgatctcaac cctgataact ctgatgtgga taagttgttc atccagctcg tgcagaccta
     3481 caaccagctt ttcgaagaga accctatcaa cgcttcaggt gtggatgcta aggctatcct
     3541 ctctgctagg ctctctaagt caagaaggct tgagaacctc attgctcagc tccctggtga
     3601 gaagaagaac ggacttttcg gaaacttgat cgctctctct ctcggactca cccctaactt
     3661 caagtctaac ttcgatctcg ctgaggatgc aaagctccag ctctcaaagg atacctacga
     3721 tgatgatctc gataacctcc tcgctcagat cggagatcag tacgctgatt tgttcctcgc
     3781 tgctaagaac ctctctgatg ctatcctcct cagtgatatc ctcagagtga acaccgagat
     3841 caccaaggct ccactctcag cttctatgat caagagatac gatgagcacc accaggatct
     3901 cacacttctc aaggctcttg ttagacagca gctcccagag aagtacaaag agattttctt
     3961 cgatcagtct aagaacggat acgctggtta catcgatggt ggtgcatctc aagaagagtt
     4021 ctacaagttc atcaagccta tcctcgagaa gatggatgga accgaggaac tcctcgtgaa
     4081 gctcaataga gaggatcttc tcagaaagca gaggaccttc gataacggat ctatccctca
     4141 tcagatccac ctcggagagt tgcacgctat ccttagaagg caagaggatt tctacccatt
     4201 cctcaaggat aacagggaaa agattgagaa gattctcacc ttcagaatcc cttactacgt
     4261 gggacctctc gctagaggaa actcaagatt cgcttggatg accagaaagt ctgaggaaac
     4321 catcacccct tggaacttcg aagaggtggt ggataagggt gctagtgctc agtctttcat
     4381 cgagaggatg accaacttcg ataagaacct tccaaacgag aaggtgctcc ctaagcactc
     4441 tttgctctac gagtacttca ccgtgtacaa cgagttgacc aaggttaagt acgtgaccga
     4501 gggaatgagg aagcctgctt ttttgtcagg tgagcaaaag aaggctatcg ttgatctctt
     4561 gttcaagacc aacagaaagg tgaccgtgaa gcagctcaaa gaggattact tcaagaaaat
     4621 cgagtgcttc gattcagttg agatttctgg tgttgaggat aggttcaacg catctctcgg
     4681 aacctaccac gatctcctca agatcattaa ggataaggat ttcttggata acgaggaaaa
     4741 cgaggatatc ttggaggata tcgttcttac cctcaccctc tttgaagata gagagatgat
     4801 tgaagaaagg ctcaagacct acgctcatct cttcgatgat aaggtgatga agcagttgaa
     4861 gagaagaaga tacactggtt ggggaaggct ctcaagaaag ctcattaacg gaatcaggga
     4921 taagcagtct ggaaagacaa tccttgattt cctcaagtct gatggattcg ctaacagaaa
     4981 cttcatgcag ctcatccacg atgattctct cacctttaaa gaggatatcc agaaggctca
     5041 ggtttcagga cagggtgata gtctccatga gcatatcgct aacctcgctg gatctcctgc
     5101 aatcaagaag ggaatcctcc agactgtgaa ggttgtggat gagttggtga aggtgatggg
     5161 aaggcataag cctgagaaca tcgtgatcga aatggctaga gagaaccaga ccactcagaa
     5221 gggacagaag aactctaggg aaaggatgaa gaggatcgag gaaggtatca aagagcttgg
     5281 atctcagatc ctcaaagagc accctgttga gaacactcag ctccagaatg agaagctcta
     5341 cctctactac ctccagaacg gaagggatat gtatgtggat caagagttgg atatcaacag
     5401 gctctctgat tacgatgttg atcatatcgt gccacagtca ttcttgaagg atgattctat
     5461 cgataacaag gtgctcacca ggtctgataa gaacaggggt aagagtgata acgtgccaag
     5521 tgaagaggtt gtgaagaaaa tgaagaacta ttggaggcag ctcctcaacg ctaagctcat
     5581 cactcagaga aagttcgata acttgactaa ggctgagagg ggaggactct ctgaattgga
     5641 taaggcagga ttcatcaaga ggcagcttgt ggaaaccagg cagatcacta agcacgttgc
     5701 acagatcctc gattctagga tgaacaccaa gtacgatgag aacgataagt tgatcaggga
     5761 agtgaaggtt atcaccctca agtcaaagct cgtgtctgat ttcagaaagg atttccaatt
     5821 ctacaaggtg agggaaatca acaactacca ccacgctcac gatgcttacc ttaacgctgt
     5881 tgttggaacc gctctcatca agaagtatcc taagctcgag tcagagttcg tgtacggtga
     5941 ttacaaggtg tacgatgtga ggaagatgat cgctaagtct gagcaagaga tcggaaaggc
     6001 taccgctaag tatttcttct actctaacat catgaatttc ttcaagaccg agattaccct
     6061 cgctaacggt gagatcagaa agaggccact catcgagaca aacggtgaaa caggtgagat
     6121 cgtgtgggat aagggaaggg atttcgctac cgttagaaag gtgctctcta tgccacaggt
     6181 gaacatcgtt aagaaaaccg aggtgcagac cggtggattc tctaaagagt ctatcctccc
     6241 taagaggaac tctgataagc tcattgctag gaagaaggat tgggacccta agaaatacgg
     6301 tggtttcgat tctcctaccg tggcttactc tgttctcgtt gtggctaagg ttgagaaggg
     6361 aaagagtaag aagctcaagt ctgttaagga acttctcgga atcactatca tggaaaggtc
     6421 atctttcgag aagaacccaa tcgatttcct cgaggctaag ggatacaaag aggttaagaa
     6481 ggatctcatc atcaagctcc caaagtactc actcttcgaa ctcgagaacg gtagaaagag
     6541 gatgctcgct tctgctggtg agcttcaaaa gggaaacgag cttgctctcc catctaagta
     6601 cgttaacttt ctttacctcg cttctcacta cgagaagttg aagggatctc cagaagataa
     6661 cgagcagaag caacttttcg ttgagcagca caagcactac ttggatgaga tcatcgagca
     6721 gatctctgag ttctctaaaa gggtgatcct cgctgatgca aacctcgata aggtgttgtc
     6781 tgcttacaac aagcacagag ataagcctat cagggaacag gcagagaaca tcatccatct
     6841 cttcaccctt accaacctcg gtgctcctgc tgctttcaag tacttcgata caaccatcga
     6901 taggaagaga tacacctcta ccaaagaagt gctcgatgct accctcatcc atcagtctat
     6961 cactggactc tacgagacta ggatcgatct ctcacagctc ggtggtgatt caggtaagag
     7021 gccggcggcc accaagaagg ccggccaagc caagaagaag aagacaagag attcaggagg
     7081 atccaccaac ctctccgaca tcatcgagaa ggagactggc aagcagctgg tgatccagga
     7141 gagcatcttg atgctgcctg aggaggtgga ggaggtcatc ggcaacaagc cggagtccga
     7201 catcctcgtc cacaccgcct acgacgagtc caccgacgag aacgtcatgc tcctcacctc
     7261 agatgctcca gagtacaagc cctgggcctt ggtgatccaa gactccaacg gcgagaacaa
     7321 gatcaagatg ctctccggcg gcagccccaa gaagaagagg aaggttgcta ctaatttttc
     7381 tcttcttaag caagctggag atgttgaaga aaatcctgga cccatggtga gcaagggcga
     7441 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     7501 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     7561 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcac
     7621 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     7681 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     7741 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     7801 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     7861 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     7921 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     7981 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     8041 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     8101 cgccgccggg atcactcacg gcatggacga gctgtacaag tcaggttctg gttcaggttc
     8161 aggttctgga tctggaagtg gatctggatc agcggccgca tctgaattca agcgtgaaga
     8221 gcaagcaagg aaagctaagg tgaacaatga gaaaaagacg gaaatagtga aaccagagag
     8281 ttgtagcaat gaaggagatg tcaaggatct gaaaagaaag gactctgagg atggaaacga
     8341 gggtgaggaa gaagaagctt cttcgaaacc gaaaaagcca aaagttgctc tttctcatct
     8401 tcaggacatt gacgacacag aagctgatca agaagaagag taactgcccc cgatgagcta
     8461 agctagctat atcatcaatt tatgtattac acataatatc gcactcagtc tttcatctac
     8521 ggcaatgtac cagctgatat aatcagttat tgaaatattt ctgaatttaa acttgcatca
     8581 ataaatttat gtttttgctt ggactataat acctgacttg ttattttatc aataaatatt
     8641 taaactatat ttctttcaag atgggaatta acatctacaa attgcctttt cttatcgacc
     8701 atgtactcga cggccgagta ctataagcta ggtagtaact agtgcgactg tctagaatcg
     8761 cgtatgcgag tgtatgagct cgatatcccc tatagtgagt cgtattacat ggtcatagct
     8821 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     8881 ataatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     8941 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     9001 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     9061 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     9121 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     9181 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     9241 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     9301 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     9361 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     9421 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     9481 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     9541 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     9601 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     9661 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     9721 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     9781 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     9841 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9901 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9961 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10021 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    10081 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    10141 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    10201 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    10261 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10321 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    10381 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    10441 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10501 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    10561 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    10621 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    10681 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    10741 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
    10801 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
    10861 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
    10921 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
    10981 gaaaggccca gacttccgac tgagcctttc gttttatttg atgcctggca gttccctact
    11041 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
    11101 cttaagctcg ggcccacct
//
