LOCUS       pZmUBI-BP-TadA9-6xGGGGS-TaLbCas12a(D156R-D832A)-BP-G7T       11315 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...ct-en4mbdl
VERSION     urn.local...ct-en4mbdl
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..864
                     /regulatory_class="promoter"
                     /gene="CaMV 35S"
                     /note="internal BsaI recognition site removed"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:6v-dgycryx"">pCaMV 35S</a>)"
                     /label="p35S"
     CDS             899..1606
                     /created_by="User"
                     /modified_by="User"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:3a-c4nnd56"">mCherry</a>"
                     /label="mCherry"
     Linker          1610..1657
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker
                     (<a href=""urn:local:thjac:1g-c4nnd54"">(GS)8</a>)"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             1658..1906
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      1914..2181
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /label="NOST"
     promoter        2210..4195
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/1;
                     ZmUBIL.2 (<a
                     href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     intron          3187..4196
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     NLS             4203..4253
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     CDS             4254..4277
                     /created_by="chgai"
                     /label="2xSGGS"
     CDS             4278..4775
                     /standard_name="TadAmax10 (ABE8)"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TadA9"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:bn-ecuitju"">TadA9</a>"
                     /label="TadA9"
     Linker          4776..4865
                     /modified_by="mamee"
                     /label="linker"
     CDS             4872..8555
                     /%_Identity=100
                     /Motif="ATGAGCAAGCTGGAGAAGTTCACCAACTGCTACTCACTCTCCAAGACCCT
                     CCGCTTCAAGGCCATCCCCGTCGGCAAGACCCAGGAGAACATCGACAACAAGAGGCTG
                     CTGGTGGAGGATGAGAAGAGGGCCGAGGACTACAAGGGCGTCAAGAAGCTCCTCGACC
                     GGTACTACCTCTCATTCATCAATGATGTCCTCCACTCCATCAAGCTGAAGAACCTCAA
                     CAACTACATCTCCCTCTTCCGCAAGAAGACAAGGACCGAGAAGGAGAACAAGGAGCTG
                     GAGAACCTGGAGATCAACCTCCGCAAGGAGATCGCCAAGGCCTTCAAGGGCAACGAGG
                     GCTACAAGAGCCTCTTCAAGAAGGACATCATCGAGACGATCCTACCGGAGTTCCTGGA
                     TGACAAGGACGAGATCGCCCTGGTGAACAGCTTCAACGGCTTCACCACCGCCTTCACC
                     GGCTTCTTCCGCAACCGGGAGAACATGTTCTCCGAGGAGGCCAAGAGCACCTCCATCG
                     CCTTCAGGTGCATCAACGAGAACCTCACCCGCTACATCTCAAACATGGACATCTTCGA
                     GAAGGTGGACGCCATCTTCGACAAGCATGAGGTCCAGGAGATCAAGGAGAAGATCCTG
                     AACTCCGACTACGACGTGGAGGACTTCTTCGAGGGCGAGTTCTTCAACTTCGTCCTGA
                     CCCAAGAAGGCATCGACGTCTACAACGCCATCATCGGCGGCTTCGTCACCGAGTCAGG
                     TGAGAAGATCAAGGGCCTCAACGAGTACATCAACCTCTACAACCAGAAGACCAAGCAG
                     AAGCTCCCCAAGTTCAAGCCCCTCTACAAGCAGGTGCTCTCCGACCGTGAGAGCTTGA
                     GCTTCTACGGCGAGGGCTACACCTCCGACGAGGAGGTGCTGGAGGTCTTCCGCAACAC
                     CCTCAACAAGAACTCAGAGATCTTCTCCTCCATCAAGAAGCTGGAGAAGCTCTTCAAG
                     AACTTCGACGAGTACTCCTCCGCCGGCATCTTCGTGAAGAACGGCCCGGCCATCTCAA
                     CCATCTCCAAGGACATCTTCGGCGAGTGGAACGTCATCCGTGACAAGTGGAACGCCGA
                     GTACGACGACATCCACCTCAAGAAGAAGGCCGTGGTGACCGAGAAGTACGAGGACGAC
                     CGCCGCAAGAGCTTCAAGAAGATCGGCAGCTTCTCACTGGAGCAGCTCCAGGAGTACG
                     CCGACGCCGACCTCTCCGTGGTGGAGAAGCTGAAGGAGATCATCATCCAGAAGGTGGA
                     CGAGATCTACAAGGTGTACGGCAGCTCTGAGAAGCTCTTCGACGCCGACTTCGTCCTG
                     GAGAAGAGCCTCAAGAAGAACGACGCCGTGGTGGCCATCATGAAGGACCTCCTCGACT
                     CAGTCAAGAGCTTCGAGAACTACATCAAGGCCTTCTTCGGCGAGGGCAAGGAGACAAA
                     CCGCGACGAGAGCTTCTACGGCGACTTCGTCCTCGCCTATGACATCCTCCTCAAGGTG
                     GACCACATCTACGACGCCATCAGGAACTACGTGACCCAGAAGCCCTACTCAAAGGACA
                     AGTTCAAGCTCTACTTCCAGAACCCCCAGTTCATGGGCGGCTGGGACAAGGACAAGGA
                     GACTGACTACCGCGCCACCATCCTCCGCTACGGCTCAAAGTACTACCTCGCCATCATG
                     GACAAGAAGTACGCCAAGTGCCTCCAGAAGATCGACAAGGATGATGTGAACGGCAACT
                     ACGAGAAGATCAACTACAAGCTCCTCCCCGGCCCCAACAAGATGCTACCCAAGGTCTT
                     CTTCTCCAAGAAGTGGATGGCCTACTACAACCCCTCCGAGGACATCCAGAAGATCTAC
                     AAGAACGGCACCTTCAAGAAGGGTGACATGTTCAACCTCAACGACTGCCACAAGCTGA
                     TCGACTTCTTCAAGGACAGCATCTCAAGGTACCCCAAGTGGAGCAACGCCTACGACTT
                     CAACTTCTCTGAGACGGAGAAGTACAAGGACATCGCCGGCTTCTACCGGGAGGTGGAG
                     GAGCAAGGCTACAAGGTGAGCTTCGAGTCCGCCTCCAAGAAGGAGGTGGACAAGCTGG
                     TGGAGGAGGGCAAGCTCTACATGTTCCAGATCTACAACAAGGACTTCTCTGACAAGAG
                     CCACGGCACCCCCAACCTACACACCATGTACTTCAAGCTCCTCTTCGACGAGAACAAC
                     CACGGCCAGATCCGCCTCTCCGGCGGCGCCGAGCTCTTCATGAGGAGGGCCTCCCTCA
                     AGAAGGAGGAGCTGGTGGTTCACCCGGCCAACAGCCCCATCGCCAACAAGAACCCTGA
                     CAACCCCAAGAAGACCACCACCCTCTCCTACGACGTCTACAAGGACAAGAGGTTCTCA
                     GAAGACCAGTACGAGCTCCACATCCCCATCGCCATCAACAAGTGCCCCAAGAACATCT
                     TCAAGATCAACACCGAGGTGAGGGTGCTACTCAAGCATGATGATAACCCCTACGTCAT
                     CGGCATCGCCCGCGGCGAGAGGAACCTCCTCTACATCGTGGTGGTGGACGGCAAGGGC
                     AACATCGTGGAGCAGTACTCCTTGAACGAGATCATCAACAACTTCAACGGCATCAGGA
                     TCAAGACCGACTACCACTCACTCCTTGACAAGAAGGAGAAGGAGAGGTTCGAGGCCCG
                     GCAAAATTGGACAAGCATCGAGAACATCAAGGAGCTGAAGGCCGGCTACATCTCCCAG
                     GTGGTGCACAAGATCTGTGAGCTGGTGGAGAAGTACGACGCCGTCATCGCCCTGGAGG
                     ACCTCAACAGCGGCTTCAAGAACTCAAGGGTCAAGGTGGAGAAGCAGGTGTACCAGAA
                     GTTCGAGAAGATGCTCATCGACAAGCTGAACTACATGGTGGACAAGAAGAGCAACCCC
                     TGCGCCACCGGCGGCGCCCTCAAGGGCTACCAGATCACCAACAAGTTTGAGAGCTTCA
                     AGAGCATGTCAACCCAGAACGGCTTCATCTTCTACATCCCGGCCTGGCTCACCTCCAA
                     GATCGACCCCTCCACCGGCTTCGTCAACCTCCTCAAGACCAAGTACACCTCCATAGCC
                     GACAGCAAGAAGTTCATCTCCTCCTTCGACCGCATCATGTACGTCCCTGAGGAGGACC
                     TCTTCGAGTTCGCCCTCGACTACAAGAACTTCTCAAGGACCGACGCCGACTACATCAA
                     GAAGTGGAAGCTCTACAGCTACGGCAACCGCATCAGGATCTTCAGGAACCCCAAGAAG
                     AACAACGTCTTCGACTGGGAGGAGGTGTGCCTGACCTCCGCCTACAAGGAGCTCTTCA
                     ACAAGTACGGCATCAACTACCAGCAAGGTGACATCAGGGCGCTCCTATGCGAGCAGAG
                     CGACAAGGCCTTCTACTCCTCCTTCATGGCCCTCATGAGCTTGATGCTCCAGATGAGG
                     AACAGCATCACCGGCCGCACCGACGTCGACTTCCTCATCTCCCCCGTCAAGAACTCTG
                     ATGGCATCTTCTACGACTCAAGGAACTACGAGGCCCAGGAGAACGCCATCCTCCCCAA
                     GAACGCCGACGCCAACGGCGCCTACAACATCGCCAGGAAGGTGCTCTGGGCCATCGGC
                     CAGTTCAAGAAGGCCGAGGATGAGAAGCTGGACAAGGTGAAGATCGCCATCTCAAACA
                     AGGAGTGGCTGGAGTACGCCCAGACCTCCGTCAAGCAC"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TaLbCas12a(D156R-D832A)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:qc-ecppbux"">TaLbCas12a(D156R-D832A
                     )</a>"
                     /label="TaLbCas12a(D156R-D832A)"
     NLS             8562..8612
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     terminator      8620..8891
                     /label="G7T"
     Linker          8896..8944
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(9005..9025)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        9086..9136
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    9137..9946
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      10093..10681
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          11280..11300
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ccgatccatt cggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
       61 tggttagaga ggcttacgca gcacgtctca tcaagacgat ctacccgagc aataatctcc
      121 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
      181 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
      241 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
      301 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
      361 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
      421 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
      481 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
      541 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
      601 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
      661 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      721 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      781 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      841 tggagagaac acgggggacg agctaacagt attcagtcga ctggtaccaa caggctccat
      901 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      961 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1021 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1081 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1141 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1201 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     1261 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1321 gcagaagaag acgatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1381 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1441 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1501 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     1561 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtcag gttctggttc
     1621 aggttcaggt tctggatctg gaagtggatc tggatcagcg gccgcatctg aattcaagcg
     1681 tgaagagcaa gcaaggaaag ctaaggtgaa caatgagaaa aagacggaaa tagtgaaacc
     1741 agagagttgt agcaatgaag gagatgtcaa ggatctgaaa agaaaggact ctgaggatgg
     1801 aaacgagggt gaggaagaag aagcttcttc gaaaccgaaa aagccaaaag ttgctctttc
     1861 tcatcttcag gacattgacg acacagaagc tgatcaagaa gaagagtaac tgcctagagt
     1921 caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     1981 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     2041 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     2101 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     2161 tcatctatgt tactagatcg aactataagc taggtagtaa ctagtacctg tgcagcgtga
     2221 cccggtcgtg cccctctcta gagataatga gcattgcatg tctaagttat aaaaaattac
     2281 cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta tacatatatt
     2341 taaactttac tctacgaata atataatcta tagtactaca ataatatcag tgttttagag
     2401 aatcatataa atgaacagtt agacatggtc taaaggacaa ttgagtattt tgacaacagg
     2461 actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc aaatagcttc
     2521 acctatataa tacttcatcc attttattag tacatccatt tagggtttag ggttaatggt
     2581 ttttatagac taattttttt agtacatcta ttttattcta ttttagcctc taaattaaga
     2641 aaactaaaac tctattttag tttttttatt taataattta gatataaaat agaataaaat
     2701 aaagtgacta aaaattaaac aaataccctt taagaaatta aaaaaactaa ggaaacattt
     2761 ttcttgtttc gagtagataa tgccagcctg ttaaacgccg tcgacgagtc taacggacac
     2821 caaccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg
     2881 tcgctgcctc tggacccctc tcgagagttc cgctccaccg ttggacttgc tccgctgtcg
     2941 gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg cggcctcctc
     3001 ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct
     3061 tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt
     3121 gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc
     3181 ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt
     3241 ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt
     3301 ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt
     3361 tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc
     3421 cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct
     3481 tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt
     3541 tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt
     3601 ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata
     3661 ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt
     3721 tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat
     3781 gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact
     3841 acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg
     3901 agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac
     3961 tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct
     4021 atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat
     4081 ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg
     4141 cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt ctgcaaacaa
     4201 tgaaaagaac cgccgacggc agcgaattcg agcctaagaa gaagaggaag gtttccggcg
     4261 gcagctcagg aggttcctct gaggtggagt tttcccacga gtactggatg agacatgccc
     4321 tgaccctggc caagagggca cgggatgaga gggaggtgcc tgtgggagcc gtgctggtgc
     4381 tgaacaatag agtgatcggc gagggctgga acagagccat cggcctgcac gacccaacag
     4441 cccatgccga aattatggcc ctgagacagg gcggcctggt catgcagaac tacagactga
     4501 ttgacgccac cctgtactcg acattcgagc cttgcgtgat gtgcgccggc gccatgatcc
     4561 actctaggat cggccgcgtg gtgtttggcg tgaggaactc aaaaagaggc gccgcaggct
     4621 ccctgatgaa cgtgctgaac taccccggca tgaatcaccg cgtcgaaatt accgagggaa
     4681 tcctggcaga tgaatgtgcc gccctgctgt gcgatttcta tcggatgcct agacgggtgt
     4741 tcaatgctca gaagaaggcc cagagctcca tcaacggtgg cggaggttct ggcggtggag
     4801 gatccggagg tggcgggtcc ggtggtggcg gatcaggtgg aggaggttcg ggaggcggag
     4861 gtagtggctc catgagcaag ctggagaagt tcaccaactg ctactcactc tccaagaccc
     4921 tccgcttcaa ggccatcccc gtcggcaaga cccaggagaa catcgacaac aagaggctgc
     4981 tggtggagga tgagaagagg gccgaggact acaagggcgt caagaagctc ctcgaccggt
     5041 actacctctc attcatcaat gatgtcctcc actccatcaa gctgaagaac ctcaacaact
     5101 acatctccct cttccgcaag aagacaagga ccgagaagga gaacaaggag ctggagaacc
     5161 tggagatcaa cctccgcaag gagatcgcca aggccttcaa gggcaacgag ggctacaaga
     5221 gcctcttcaa gaaggacatc atcgagacga tcctaccgga gttcctggat gacaaggacg
     5281 agatcgccct ggtgaacagc ttcaacggct tcaccaccgc cttcaccggc ttcttccgca
     5341 accgggagaa catgttctcc gaggaggcca agagcacctc catcgccttc aggtgcatca
     5401 acgagaacct cacccgctac atctcaaaca tggacatctt cgagaaggtg gacgccatct
     5461 tcgacaagca tgaggtccag gagatcaagg agaagatcct gaactccgac tacgacgtgg
     5521 aggacttctt cgagggcgag ttcttcaact tcgtcctgac ccaagaaggc atcgacgtct
     5581 acaacgccat catcggcggc ttcgtcaccg agtcaggtga gaagatcaag ggcctcaacg
     5641 agtacatcaa cctctacaac cagaagacca agcagaagct ccccaagttc aagcccctct
     5701 acaagcaggt gctctccgac cgtgagagct tgagcttcta cggcgagggc tacacctccg
     5761 acgaggaggt gctggaggtc ttccgcaaca ccctcaacaa gaactcagag atcttctcct
     5821 ccatcaagaa gctggagaag ctcttcaaga acttcgacga gtactcctcc gccggcatct
     5881 tcgtgaagaa cggcccggcc atctcaacca tctccaagga catcttcggc gagtggaacg
     5941 tcatccgtga caagtggaac gccgagtacg acgacatcca cctcaagaag aaggccgtgg
     6001 tgaccgagaa gtacgaggac gaccgccgca agagcttcaa gaagatcggc agcttctcac
     6061 tggagcagct ccaggagtac gccgacgccg acctctccgt ggtggagaag ctgaaggaga
     6121 tcatcatcca gaaggtggac gagatctaca aggtgtacgg cagctctgag aagctcttcg
     6181 acgccgactt cgtcctggag aagagcctca agaagaacga cgccgtggtg gccatcatga
     6241 aggacctcct cgactcagtc aagagcttcg agaactacat caaggccttc ttcggcgagg
     6301 gcaaggagac aaaccgcgac gagagcttct acggcgactt cgtcctcgcc tatgacatcc
     6361 tcctcaaggt ggaccacatc tacgacgcca tcaggaacta cgtgacccag aagccctact
     6421 caaaggacaa gttcaagctc tacttccaga acccccagtt catgggcggc tgggacaagg
     6481 acaaggagac tgactaccgc gccaccatcc tccgctacgg ctcaaagtac tacctcgcca
     6541 tcatggacaa gaagtacgcc aagtgcctcc agaagatcga caaggatgat gtgaacggca
     6601 actacgagaa gatcaactac aagctcctcc ccggccccaa caagatgcta cccaaggtct
     6661 tcttctccaa gaagtggatg gcctactaca acccctccga ggacatccag aagatctaca
     6721 agaacggcac cttcaagaag ggtgacatgt tcaacctcaa cgactgccac aagctgatcg
     6781 acttcttcaa ggacagcatc tcaaggtacc ccaagtggag caacgcctac gacttcaact
     6841 tctctgagac ggagaagtac aaggacatcg ccggcttcta ccgggaggtg gaggagcaag
     6901 gctacaaggt gagcttcgag tccgcctcca agaaggaggt ggacaagctg gtggaggagg
     6961 gcaagctcta catgttccag atctacaaca aggacttctc tgacaagagc cacggcaccc
     7021 ccaacctaca caccatgtac ttcaagctcc tcttcgacga gaacaaccac ggccagatcc
     7081 gcctctccgg cggcgccgag ctcttcatga ggagggcctc cctcaagaag gaggagctgg
     7141 tggttcaccc ggccaacagc cccatcgcca acaagaaccc tgacaacccc aagaagacca
     7201 ccaccctctc ctacgacgtc tacaaggaca agaggttctc agaagaccag tacgagctcc
     7261 acatccccat cgccatcaac aagtgcccca agaacatctt caagatcaac accgaggtga
     7321 gggtgctact caagcatgat gataacccct acgtcatcgg catcgcccgc ggcgagagga
     7381 acctcctcta catcgtggtg gtggacggca agggcaacat cgtggagcag tactccttga
     7441 acgagatcat caacaacttc aacggcatca ggatcaagac cgactaccac tcactccttg
     7501 acaagaagga gaaggagagg ttcgaggccc ggcaaaattg gacaagcatc gagaacatca
     7561 aggagctgaa ggccggctac atctcccagg tggtgcacaa gatctgtgag ctggtggaga
     7621 agtacgacgc cgtcatcgcc ctggaggacc tcaacagcgg cttcaagaac tcaagggtca
     7681 aggtggagaa gcaggtgtac cagaagttcg agaagatgct catcgacaag ctgaactaca
     7741 tggtggacaa gaagagcaac ccctgcgcca ccggcggcgc cctcaagggc taccagatca
     7801 ccaacaagtt tgagagcttc aagagcatgt caacccagaa cggcttcatc ttctacatcc
     7861 cggcctggct cacctccaag atcgacccct ccaccggctt cgtcaacctc ctcaagacca
     7921 agtacacctc catagccgac agcaagaagt tcatctcctc cttcgaccgc atcatgtacg
     7981 tccctgagga ggacctcttc gagttcgccc tcgactacaa gaacttctca aggaccgacg
     8041 ccgactacat caagaagtgg aagctctaca gctacggcaa ccgcatcagg atcttcagga
     8101 accccaagaa gaacaacgtc ttcgactggg aggaggtgtg cctgacctcc gcctacaagg
     8161 agctcttcaa caagtacggc atcaactacc agcaaggtga catcagggcg ctcctatgcg
     8221 agcagagcga caaggccttc tactcctcct tcatggccct catgagcttg atgctccaga
     8281 tgaggaacag catcaccggc cgcaccgacg tcgacttcct catctccccc gtcaagaact
     8341 ctgatggcat cttctacgac tcaaggaact acgaggccca ggagaacgcc atcctcccca
     8401 agaacgccga cgccaacggc gcctacaaca tcgccaggaa ggtgctctgg gccatcggcc
     8461 agttcaagaa ggccgaggat gagaagctgg acaaggtgaa gatcgccatc tcaaacaagg
     8521 agtggctgga gtacgcccag acctccgtca agcactcagg taaaagaacc gccgacggca
     8581 gcgaattcga gcctaagaag aagaggaagg tttgactgcc cccgatgagc taagctagct
     8641 atatcatcaa tttatgtatt acacataata tcgcactcag tctttcatct acggcaatgt
     8701 accagctgat ataatcagtt attgaaatat ttctgaattt aaacttgcat caataaattt
     8761 atgtttttgc ttggactata atacctgact tgttatttta tcaataaata tttaaactat
     8821 atttctttca agatgggaat taacatctac aaattgcctt ttcttatcga ccatgtactc
     8881 gacggccgag tactataagc taggtagtaa ctagtgcgac tgtctagaat cgcgtatgcg
     8941 agtgtatcta gaatcgcgta tgcgagtgta tgagctcgat atcccctata gtgagtcgta
     9001 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     9061 cattgcacaa gataaaataa tatcatcatg aacaataaaa ctgtctgctt acataaacag
     9121 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     9181 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     9241 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     9301 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     9361 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     9421 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     9481 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     9541 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     9601 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     9661 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     9721 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     9781 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     9841 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     9901 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     9961 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
    10021 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
    10081 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    10141 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    10201 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    10261 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    10321 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    10381 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    10441 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    10501 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    10561 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10621 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    10681 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    10741 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    10801 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    10861 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
    10921 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac
    10981 cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
    11041 ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
    11101 ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
    11161 aaacaacaga taaaacgaaa ggcccagact tccgactgag cctttcgttt tatttgatgc
    11221 ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg
    11281 taaaacgacg gccagtctta agctcgggcc cacct
//
