LOCUS       pGG-B-BP-A3Aintron-6xGGGGS-C        3637 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...cq-en4mbdj
VERSION     urn.local...cq-en4mbdj
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     NLS             15..65
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     CDS             join(90..376,566..872)
                     /codon_start=1
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A3Aintron"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; APOBEC3A (<a
                     href=""urn:local:thjac:t-ecoeihu"">A3Aintron</a>)"
                     /label="APOBEC3A"
     intron          377..565
                     /vntifkey=15
                     /modified_by="thjac"
                     /label="IV2 intron"
     CDS             873..962
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="(GGGS)6"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:4k-ecylg1o"">(GGGS)6</a>"
                     /modified_by="mamee"
                     /label="6xGGGS"
     GG              complement(963..973)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(993..1013)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1493..1597
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1598..2458
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2629..3217
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3581..3602
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatgaaaaga accgccgacg gcagcgaatt cgagcctaag aagaagagga
       61 aggtttccgg cggcagctca ggaggttccg aagcctctcc tgcctctgga cccagacacc
      121 tgatggaccc tcacatcttc accagcaact tcaacaacgg catcggccgg cacaagacct
      181 acctgtgcta cgaggtggaa cggctggaca atggcaccag cgtgaagatg gaccagcacc
      241 ggggctttct gcacaaccag gccaagaatc tgctgtgcgg cttctacggc agacacgccg
      301 agctgagatt tctggacctg gtgcctagcc tgcagctgga ccctgctcag atctacagag
      361 tgacctggtt catcaggtaa gtttctgctt ctacctttga tatatatata ataattatca
      421 ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat
      481 agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat
      541 atgaccaaaa tttgttgatg tgcagctggt ccccatgctt ctcttggggc tgtgctggcg
      601 aagtgcgcgc cttcctgcaa gagaatactc acgtgcggct gcggatcttc gccgccagaa
      661 tctacgacta cgaccctctg tacaaagagg ccctgcagat gctgagagat gccggtgctc
      721 aggtgtccat catgacctac gacgagttca agcactgctg ggacaccttc gtggatcacc
      781 agggctgccc tttccagcct tgggatggac tggatgagca ctctcaggcc ctgagcggta
      841 gactgagagc catcctgcag aaccagggca acggtggcgg aggttctggc ggtggaggat
      901 ccggaggtgg cgggtccggt ggtggcggat caggtggagg aggttcggga ggcggaggta
      961 gtggcttgag accgaattct cgccctatag tgagtcgtat tacctggccg tcgttttaca
     1021 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     1081 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     1141 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     1201 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     1261 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     1321 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     1381 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     1441 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     1501 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1561 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1621 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1681 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     1741 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1801 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     1861 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1921 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1981 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     2041 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2101 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     2161 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2221 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2281 tattgctgat aaatctggag ccggtgagcg tggctctcga ggtatcattg cagcactggg
     2341 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2401 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2461 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2521 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2581 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2641 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2701 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2761 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2821 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2881 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2941 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3001 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3061 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3301 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     3421 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     3481 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     3541 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     3601 cacgagctat ttaggtgaca ctatagaagt gaagctt
//
