LOCUS       pGG-B-BP-TadA8e-6xGGGGS-C        3352 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...cp-en4mbdj
VERSION     urn.local...cp-en4mbdj
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     NLS             15..65
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     CDS             66..89
                     /created_by="chgai"
                     /label="2xSGGS"
     CDS             90..587
                     /standard_name="TadAmax10 (ABE8)"
                     /label="TadAmax10 (ABE8)"
     CDS             588..677
                     /modified_by="mamee"
                     /label="6xGGGS"
     GG              complement(678..688)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(708..728)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1208..1312
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1313..2173
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2344..2932
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3296..3317
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatgaaaaga accgccgacg gcagcgaatt cgagcctaag aagaagagga
       61 aggtttccgg cggcagctca ggaggttcct ctgaggtgga gttttcccac gagtactgga
      121 tgagacatgc cctgaccctg gccaagaggg cacgggatga gagggaggtg cctgtgggag
      181 ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc atcggcctgc
      241 acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg gtcatgcaga
      301 actacagact gattgacgcc accctgtacg tgacattcga gccttgcgtg atgtgcgccg
      361 gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac tcaaaaagag
      421 gcgccgcagg ctccctgatg aacgtgctga actaccccgg catgaatcac cgcgtcgaaa
      481 ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgcgatttc tatcggatgc
      541 ctagacaggt gttcaatgct cagaagaagg cccagagctc catcaacggt ggcggaggtt
      601 ctggcggtgg aggatccgga ggtggcgggt ccggtggtgg cggatcaggt ggaggaggtt
      661 cgggaggcgg aggtagtggc ttgagaccga attctcgccc tatagtgagt cgtattacct
      721 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      781 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      841 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
      901 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
      961 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     1021 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     1081 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     1141 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     1201 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1261 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1321 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1381 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1441 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     1501 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     1561 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1621 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     1681 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     1741 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1801 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     1861 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     1921 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     1981 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggct ctcgaggtat
     2041 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2101 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2161 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2221 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2281 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2341 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2401 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     2461 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     2521 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     2581 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     2641 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2701 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2761 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2821 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2881 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2941 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3001 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3061 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3121 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     3181 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     3241 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     3301 cggataacaa tttcacacga gctatttagg tgacactata gaagtgaagc tt
//
