LOCUS       pZmUBI-BP-TadA8e-nCas9-BP-P2A-N7-NLS-G7T       10582 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...co-en4mbdj
VERSION     urn.local...co-en4mbdj
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1986
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/1;
                     ZmUBIL.2 (<a
                     href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     intron          978..1987
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     NLS             1994..2044
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     CDS             2045..2068
                     /created_by="chgai"
                     /label="2xSGGS"
     CDS             2069..2566
                     /standard_name="TadAmax10 (ABE8)"
                     /label="TadAmax10 (ABE8)"
     Linker          2567..2662
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="32-aa linker"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a3-dgycryz"">32-aa linker</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24;
                     XTEN (<a href=""urn:local:.:a3-dgycryz"">32-aa
                     linker</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24;
                     <a href=""urn:local:.:a5-dgycryz"">XTEN</a>"
                     /label="32-aa linker"
     CDS             2669..6772
                     /X-Transferred_From="Cas9PTA"
                     /X-Transferred_Similarity="99.98%"
                     /X-Primary_Match="99.98%; Adjusted-Interval-0:0/0; CAS9
                     (<a href=""urn:local:thjac:3c-c4nnd56"">Cas9PTA</a>)"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="nCAS9(D10A)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:pe-ecpo7bv"">nCAS9(D10A)</a>"
                     /label="nCAS9(D10A)"
     NLS             6779..6829
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     CDS             6830..6886
                     /created_by="User"
                     /Transferred_From="P2A"
                     /Transferred_Similarity="100.00%"
                     /label="P2A"
     CDS             6887..7603
                     /note="A206K"
                     /codon_start=1
                     /transl_table=11
                     /product="GFP"
                     /protein_id="AHE38511.1"
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="GFP"
                     /Transferred_Similarity="100.00%"
                     /label="GFP"
     Linker          7607..7654
                     /created_by="wacae"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             7655..7903
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      7911..8182
                     /label="G7T"
     Linker          8187..8235
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(8272..8292)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        8353..8403
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    8404..9213
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      9360..9948
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          10547..10567
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta
       61 taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt
      121 atacatatat ttaaacttta ctctacgaat aatataatct atagtactac aataatatca
      181 gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt
      241 ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg
      301 caaatagctt cacctatata atacttcatc cattttatta gtacatccat ttagggttta
      361 gggttaatgg tttttataga ctaatttttt tagtacatct attttattct attttagcct
      421 ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa
      481 tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta
      541 aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt
      601 ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca
      661 cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg
      721 ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag
      781 gcggcctcct cctcctctca cggcacggca gctacggggg attcctttcc caccgctcct
      841 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
      901 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
      961 ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc taccttctct
     1021 agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc atgtttgtgt
     1081 tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac
     1141 gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc
     1201 tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt
     1261 tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt
     1321 tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc
     1381 ggagtagaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg
     1441 tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata
     1501 ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg
     1561 gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac
     1621 tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct
     1681 tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat
     1741 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     1801 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     1861 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     1921 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     1981 tctgcaaaca atgaaaagaa ccgccgacgg cagcgaattc gagcctaaga agaagaggaa
     2041 ggtttccggc ggcagctcag gaggttcctc tgaggtggag ttttcccacg agtactggat
     2101 gagacatgcc ctgaccctgg ccaagagggc acgggatgag agggaggtgc ctgtgggagc
     2161 cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg aacagagcca tcggcctgca
     2221 cgacccaaca gcccatgccg aaattatggc cctgagacag ggcggcctgg tcatgcagaa
     2281 ctacagactg attgacgcca ccctgtacgt gacattcgag ccttgcgtga tgtgcgccgg
     2341 cgccatgatc cactctagga tcggccgcgt ggtgtttggc gtgaggaact caaaaagagg
     2401 cgccgcaggc tccctgatga acgtgctgaa ctaccccggc atgaatcacc gcgtcgaaat
     2461 taccgaggga atcctggcag atgaatgtgc cgccctgctg tgcgatttct atcggatgcc
     2521 tagacaggtg ttcaatgctc agaagaaggc ccagagctcc atcaactccg gaggatctag
     2581 cggaggctcc tctggctctg agacacctgg cacaagcgag agcgcaacac ctgaaagcag
     2641 cgggggcagc agcggggggt caggctcgat ggataagaag tactctatcg gactcgctat
     2701 cggaactaac tctgtgggat gggctgtgat caccgatgag tacaaggtgc catctaagaa
     2761 gttcaaggtt ctcggaaaca ccgataggca ctctatcaag aaaaacctta tcggtgctct
     2821 cctcttcgat tctggtgaaa ctgctgaggc taccagactc aagagaaccg ctagaagaag
     2881 gtacaccaga agaaagaaca ggatctgcta cctccaagag atcttctcta acgagatggc
     2941 taaagtggat gattcattct tccacaggct cgaagagtca ttcctcgtgg aagaagataa
     3001 gaagcacgag aggcacccta tcttcggaaa catcgttgat gaggtggcat accacgagaa
     3061 gtaccctact atctaccacc tcagaaagaa gctcgttgat tctactgata aggctgatct
     3121 caggctcatc tacctcgctc tcgctcacat gatcaagttc agaggacact tcctcatcga
     3181 gggtgatctc aaccctgata actctgatgt ggataagttg ttcatccagc tcgtgcagac
     3241 ctacaaccag cttttcgaag agaaccctat caacgcttca ggtgtggatg ctaaggctat
     3301 cctctctgct aggctctcta agtcaagaag gcttgagaac ctcattgctc agctccctgg
     3361 tgagaagaag aacggacttt tcggaaactt gatcgctctc tctctcggac tcacccctaa
     3421 cttcaagtct aacttcgatc tcgctgagga tgcaaagctc cagctctcaa aggataccta
     3481 cgatgatgat ctcgataacc tcctcgctca gatcggagat cagtacgctg atttgttcct
     3541 cgctgctaag aacctctctg atgctatcct cctcagtgat atcctcagag tgaacaccga
     3601 gatcaccaag gctccactct cagcttctat gatcaagaga tacgatgagc accaccagga
     3661 tctcacactt ctcaaggctc ttgttagaca gcagctccca gagaagtaca aagagatttt
     3721 cttcgatcag tctaagaacg gatacgctgg ttacatcgat ggtggtgcat ctcaagaaga
     3781 gttctacaag ttcatcaagc ctatcctcga gaagatggat ggaaccgagg aactcctcgt
     3841 gaagctcaat agagaggatc ttctcagaaa gcagaggacc ttcgataacg gatctatccc
     3901 tcatcagatc cacctcggag agttgcacgc tatccttaga aggcaagagg atttctaccc
     3961 attcctcaag gataacaggg aaaagattga gaagattctc accttcagaa tcccttacta
     4021 cgtgggacct ctcgctagag gaaactcaag attcgcttgg atgaccagaa agtctgagga
     4081 aaccatcacc ccttggaact tcgaagaggt ggtggataag ggtgctagtg ctcagtcttt
     4141 catcgagagg atgaccaact tcgataagaa ccttccaaac gagaaggtgc tccctaagca
     4201 ctctttgctc tacgagtact tcaccgtgta caacgagttg accaaggtta agtacgtgac
     4261 cgagggaatg aggaagcctg cttttttgtc aggtgagcaa aagaaggcta tcgttgatct
     4321 cttgttcaag accaacagaa aggtgaccgt gaagcagctc aaagaggatt acttcaagaa
     4381 aatcgagtgc ttcgattcag ttgagatttc tggtgttgag gataggttca acgcatctct
     4441 cggaacctac cacgatctcc tcaagatcat taaggataag gatttcttgg ataacgagga
     4501 aaacgaggat atcttggagg atatcgttct taccctcacc ctctttgaag atagagagat
     4561 gattgaagaa aggctcaaga cctacgctca tctcttcgat gataaggtga tgaagcagtt
     4621 gaagagaaga agatacactg gttggggaag gctctcaaga aagctcatta acggaatcag
     4681 ggataagcag tctggaaaga caatccttga tttcctcaag tctgatggat tcgctaacag
     4741 aaacttcatg cagctcatcc acgatgattc tctcaccttt aaagaggata tccagaaggc
     4801 tcaggtttca ggacagggtg atagtctcca tgagcatatc gctaacctcg ctggatctcc
     4861 tgcaatcaag aagggaatcc tccagactgt gaaggttgtg gatgagttgg tgaaggtgat
     4921 gggaaggcat aagcctgaga acatcgtgat cgaaatggct agagagaacc agaccactca
     4981 gaagggacag aagaactcta gggaaaggat gaagaggatc gaggaaggta tcaaagagct
     5041 tggatctcag atcctcaaag agcaccctgt tgagaacact cagctccaga atgagaagct
     5101 ctacctctac tacctccaga acggaaggga tatgtatgtg gatcaagagt tggatatcaa
     5161 caggctctct gattacgatg ttgatcatat cgtgccacag tcattcttga aggatgattc
     5221 tatcgataac aaggtgctca ccaggtctga taagaacagg ggtaagagtg ataacgtgcc
     5281 aagtgaagag gttgtgaaga aaatgaagaa ctattggagg cagctcctca acgctaagct
     5341 catcactcag agaaagttcg ataacttgac taaggctgag aggggaggac tctctgaatt
     5401 ggataaggca ggattcatca agaggcagct tgtggaaacc aggcagatca ctaagcacgt
     5461 tgcacagatc ctcgattcta ggatgaacac caagtacgat gagaacgata agttgatcag
     5521 ggaagtgaag gttatcaccc tcaagtcaaa gctcgtgtct gatttcagaa aggatttcca
     5581 attctacaag gtgagggaaa tcaacaacta ccaccacgct cacgatgctt accttaacgc
     5641 tgttgttgga accgctctca tcaagaagta tcctaagctc gagtcagagt tcgtgtacgg
     5701 tgattacaag gtgtacgatg tgaggaagat gatcgctaag tctgagcaag agatcggaaa
     5761 ggctaccgct aagtatttct tctactctaa catcatgaat ttcttcaaga ccgagattac
     5821 cctcgctaac ggtgagatca gaaagaggcc actcatcgag acaaacggtg aaacaggtga
     5881 gatcgtgtgg gataagggaa gggatttcgc taccgttaga aaggtgctct ctatgccaca
     5941 ggtgaacatc gttaagaaaa ccgaggtgca gaccggtgga ttctctaaag agtctatcct
     6001 ccctaagagg aactctgata agctcattgc taggaagaag gattgggacc ctaagaaata
     6061 cggtggtttc gattctccta ccgtggctta ctctgttctc gttgtggcta aggttgagaa
     6121 gggaaagagt aagaagctca agtctgttaa ggaacttctc ggaatcacta tcatggaaag
     6181 gtcatctttc gagaagaacc caatcgattt cctcgaggct aagggataca aagaggttaa
     6241 gaaggatctc atcatcaagc tcccaaagta ctcactcttc gaactcgaga acggtagaaa
     6301 gaggatgctc gcttctgctg gtgagcttca aaagggaaac gagcttgctc tcccatctaa
     6361 gtacgttaac tttctttacc tcgcttctca ctacgagaag ttgaagggat ctccagaaga
     6421 taacgagcag aagcaacttt tcgttgagca gcacaagcac tacttggatg agatcatcga
     6481 gcagatctct gagttctcta aaagggtgat cctcgctgat gcaaacctcg ataaggtgtt
     6541 gtctgcttac aacaagcaca gagataagcc tatcagggaa caggcagaga acatcatcca
     6601 tctcttcacc cttaccaacc tcggtgctcc tgctgctttc aagtacttcg atacaaccat
     6661 cgataggaag agatacacct ctaccaaaga agtgctcgat gctaccctca tccatcagtc
     6721 tatcactgga ctctacgaga ctaggatcga tctctcacag ctcggtggtg attcaggtaa
     6781 aagaaccgcc gacggcagcg aattcgagcc taagaagaag aggaaggttg ctactaattt
     6841 ttctcttctt aagcaagctg gagatgttga agaaaatcct ggacccatgg tgagcaaggg
     6901 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     6961 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     7021 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     7081 cacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     7141 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     7201 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     7261 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     7321 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     7381 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     7441 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     7501 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     7561 gaccgccgcc gggatcactc acggcatgga cgagctgtac aagtcaggtt ctggttcagg
     7621 ttcaggttct ggatctggaa gtggatctgg atcagcggcc gcatctgaat tcaagcgtga
     7681 agagcaagca aggaaagcta aggtgaacaa tgagaaaaag acggaaatag tgaaaccaga
     7741 gagttgtagc aatgaaggag atgtcaagga tctgaaaaga aaggactctg aggatggaaa
     7801 cgagggtgag gaagaagaag cttcttcgaa accgaaaaag ccaaaagttg ctctttctca
     7861 tcttcaggac attgacgaca cagaagctga tcaagaagaa gagtaactgc ccccgatgag
     7921 ctaagctagc tatatcatca atttatgtat tacacataat atcgcactca gtctttcatc
     7981 tacggcaatg taccagctga tataatcagt tattgaaata tttctgaatt taaacttgca
     8041 tcaataaatt tatgtttttg cttggactat aatacctgac ttgttatttt atcaataaat
     8101 atttaaacta tatttctttc aagatgggaa ttaacatcta caaattgcct tttcttatcg
     8161 accatgtact cgacggccga gtactataag ctaggtagta actagtgcga ctgtctagaa
     8221 tcgcgtatgc gagtgtatga gctcgatatc ccctatagtg agtcgtatta catggtcata
     8281 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     8341 aaaataatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     8401 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     8461 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     8521 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     8581 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     8641 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     8701 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     8761 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     8821 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     8881 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     8941 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     9001 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     9061 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     9121 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     9181 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     9241 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     9301 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9361 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9421 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9481 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9541 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9601 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9661 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9721 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9781 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9841 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9901 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9961 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    10021 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    10081 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    10141 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    10201 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
    10261 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
    10321 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
    10381 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
    10441 aacgaaaggc ccagacttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
    10501 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
    10561 agtcttaagc tcgggcccac ct
//
