LOCUS       pZmUBI-tRNA-mature-DR_crRNA-GFP-ABE-matureDR-polyT-NOST        4832 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...cm-en4mbdh
VERSION     urn.local...cm-en4mbdh
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1986
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/1;
                     ZmUBIL.2 (<a
                     href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     intron          978..1987
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     RNA_motif       1991..2054
                     /created_by="chgai"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="truncated tRNA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2l-ecoxit3"">truncated tRNA</a>"
                     /label="truncated tRNA"
     RNA_motif       2058..2078
                     /created_by="chgai"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Mature DR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Mature_DR (<a href=""urn:local:thjac:2c-ecoxhcj"">Mature
                     DR</a>)"
                     /label="Mature_DR"
     crRNA           2079..2101
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ABE-Cas12a_2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:32q-eczixvr"">ABE-Cas12a_2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:i2-ecpi3uh"">GFP(Q70stop-R74K)</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:7i-ecuewpy"">GFP(Q70stop)</a>)"
                     /label
     RNA_motif       2102..2122
                     /created_by="chgai"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Mature DR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Mature_DR (<a href=""urn:local:thjac:2c-ecoxhcj"">Mature
                     DR</a>)"
                     /label="Mature_DR"
     terminator      2165..2432
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /label="NOST"
     Linker          2437..2485
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(2522..2542)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        2603..2653
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    2654..3463
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      3610..4198
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          4797..4817
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta
       61 taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt
      121 atacatatat ttaaacttta ctctacgaat aatataatct atagtactac aataatatca
      181 gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt
      241 ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg
      301 caaatagctt cacctatata atacttcatc cattttatta gtacatccat ttagggttta
      361 gggttaatgg tttttataga ctaatttttt tagtacatct attttattct attttagcct
      421 ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa
      481 tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta
      541 aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt
      601 ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca
      661 cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg
      721 ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag
      781 gcggcctcct cctcctctca cggcacggca gctacggggg attcctttcc caccgctcct
      841 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
      901 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
      961 ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc taccttctct
     1021 agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc atgtttgtgt
     1081 tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac
     1141 gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc
     1201 tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt
     1261 tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt
     1321 tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc
     1381 ggagtagaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg
     1441 tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata
     1501 ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg
     1561 gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac
     1621 tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct
     1681 tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat
     1741 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     1801 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     1861 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     1921 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     1981 tctgcaaaca ccagtggtct agtggtagaa tagtaccctg ccacggtaca gacccgggtt
     2041 cgattcccgg ctggaaataa tttctactaa gtgtagatct gaagcactac acgccgtagg
     2101 ttaatttcta ctaagtgtag atttttttaa caggctgtat tcagtcgact ggtaccaaca
     2161 ctgcctagag tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc
     2221 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     2281 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     2341 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     2401 cgcgcgcggt gtcatctatg ttactagatc gaactataag ctaggtagta actagtgcga
     2461 ctgtctagaa tcgcgtatgc gagtgtatga gctcgatatc ccctatagtg agtcgtatta
     2521 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2581 tgcacaagat aaaataatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     2641 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     2701 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2761 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2821 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2881 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     2941 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3001 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3061 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     3121 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3181 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     3241 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3301 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3361 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3421 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3481 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3541 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3601 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3661 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3721 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3781 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3841 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3901 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3961 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4021 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4081 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4141 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4201 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4261 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4321 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4381 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     4441 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
     4501 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
     4561 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
     4621 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
     4681 caacagataa aacgaaaggc ccagacttcc gactgagcct ttcgttttat ttgatgcctg
     4741 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
     4801 aacgacggcc agtcttaagc tcgggcccac ct
//
