LOCUS       pGG-B-tRNA-matDR-BbsI-ccdB-BbsI-C        3072 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...cb-en4mbdd
VERSION     urn.local...cb-en4mbdd
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     RNA_motif       12..75
                     /created_by="chgai"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="truncated tRNA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2l-ecoxit3"">truncated tRNA</a>"
                     /label="truncated tRNA"
     RNA_motif       79..99
                     /created_by="chgai"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Mature DR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Mature_DR (<a href=""urn:local:thjac:2c-ecoxhcj"">Mature
                     DR</a>)"
                     /label="Mature_DR"
     UTR             108..>342
                     /Mismatches=1
                     /%_Identity="99.68652037617555"
                     /Motif="AAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGA
                     ATATATACTGATATGTATACCCGAAGTATGTCAAAAGGAGGTATGCTATGAAGCAGCG
                     TATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCA
                     ATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGA
                     ACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATG
                     AACGGCTCTTTTGCTGACGAGAACAGGGGCTGGTGAA"
                     /annotation_group="ccdB 5'UTR: 441 -> 759"
                     /label="ccdB 5'UTR"
     CDS             <343..385
                     /codon_start=2
                     /label="ccdB"
     GG              complement(398..408)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(428..448)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        928..1032
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1033..1893
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2064..2652
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3016..3037
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac accagtggtc tagtggtaga atagtaccct gccacggtac agacccgggt
       61 tcgattcccg gctggaaata atttctacta agtgtagatg ggtcttcaag ccagataaca
      121 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg
      181 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac
      241 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca
      301 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agtgacatca aaaacgccat
      361 taacctgatg ttctggggaa tataagaaga caaaacaggc ttgagaccga attctcgccc
      421 tatagtgagt cgtattacct ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      481 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      541 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
      601 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
      661 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
      721 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
      781 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
      841 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      901 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      961 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1021 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1081 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1141 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1201 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1261 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1321 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1381 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1441 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1501 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1561 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1621 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1681 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     1741 gagcgtggct ctcgaggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     1801 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1861 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1921 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1981 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2041 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2101 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2161 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     2221 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2281 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2341 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2401 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2461 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2521 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2581 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2641 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2701 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2761 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2821 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2881 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2941 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     3001 atgttgtgtg gaattgtgag cggataacaa tttcacacga gctatttagg tgacactata
     3061 gaagtgaagc tt
//
