LOCUS       pZmUBI-BP-TadA8e-TaLbCas12a(D832A)-BP-P2A-GFP-N7-G7T       10162 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...bt-en4mbd6
VERSION     urn.local...bt-en4mbd6
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1986
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/1;
                     ZmUBIL.2 (<a
                     href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     intron          978..1987
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     NLS             1994..2044
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     CDS             2045..2068
                     /created_by="chgai"
                     /label="2xSGGS"
     CDS             2069..2566
                     /standard_name="TadAmax10 (ABE8)"
                     /label="TadAmax10 (ABE8)"
     Linker          2567..2662
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="32-aa linker"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a3-dgycryz"">32-aa linker</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24;
                     XTEN (<a href=""urn:local:.:a3-dgycryz"">32-aa
                     linker</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24;
                     <a href=""urn:local:.:a5-dgycryz"">XTEN</a>"
                     /label="32-aa linker"
     CDS             2669..6352
                     /%_Identity="99.9457111834962"
                     /Motif="ATGAGCAAGCTGGAGAAGTTCACCAACTGCTACTCACTCTCCAAGACCCT
                     CCGCTTCAAGGCCATCCCCGTCGGCAAGACCCAGGAGAACATCGACAACAAGAGGCTG
                     CTGGTGGAGGATGAGAAGAGGGCCGAGGACTACAAGGGCGTCAAGAAGCTCCTCGACC
                     GGTACTACCTCTCATTCATCAATGATGTCCTCCACTCCATCAAGCTGAAGAACCTCAA
                     CAACTACATCTCCCTCTTCCGCAAGAAGACAAGGACCGAGAAGGAGAACAAGGAGCTG
                     GAGAACCTGGAGATCAACCTCCGCAAGGAGATCGCCAAGGCCTTCAAGGGCAACGAGG
                     GCTACAAGAGCCTCTTCAAGAAGGACATCATCGAGACGATCCTACCGGAGTTCCTGGA
                     TGACAAGGACGAGATCGCCCTGGTGAACAGCTTCAACGGCTTCACCACCGCCTTCACC
                     GGCTTCTTCCGCAACCGGGAGAACATGTTCTCCGAGGAGGCCAAGAGCACCTCCATCG
                     CCTTCAGGTGCATCAACGAGAACCTCACCCGCTACATCTCAAACATGGACATCTTCGA
                     GAAGGTGGACGCCATCTTCGACAAGCATGAGGTCCAGGAGATCAAGGAGAAGATCCTG
                     AACTCCGACTACGACGTGGAGGACTTCTTCGAGGGCGAGTTCTTCAACTTCGTCCTGA
                     CCCAAGAAGGCATCGACGTCTACAACGCCATCATCGGCGGCTTCGTCACCGAGTCAGG
                     TGAGAAGATCAAGGGCCTCAACGAGTACATCAACCTCTACAACCAGAAGACCAAGCAG
                     AAGCTCCCCAAGTTCAAGCCCCTCTACAAGCAGGTGCTCTCCGACCGTGAGAGCTTGA
                     GCTTCTACGGCGAGGGCTACACCTCCGACGAGGAGGTGCTGGAGGTCTTCCGCAACAC
                     CCTCAACAAGAACTCAGAGATCTTCTCCTCCATCAAGAAGCTGGAGAAGCTCTTCAAG
                     AACTTCGACGAGTACTCCTCCGCCGGCATCTTCGTGAAGAACGGCCCGGCCATCTCAA
                     CCATCTCCAAGGACATCTTCGGCGAGTGGAACGTCATCCGTGACAAGTGGAACGCCGA
                     GTACGACGACATCCACCTCAAGAAGAAGGCCGTGGTGACCGAGAAGTACGAGGACGAC
                     CGCCGCAAGAGCTTCAAGAAGATCGGCAGCTTCTCACTGGAGCAGCTCCAGGAGTACG
                     CCGACGCCGACCTCTCCGTGGTGGAGAAGCTGAAGGAGATCATCATCCAGAAGGTGGA
                     CGAGATCTACAAGGTGTACGGCAGCTCTGAGAAGCTCTTCGACGCCGACTTCGTCCTG
                     GAGAAGAGCCTCAAGAAGAACGACGCCGTGGTGGCCATCATGAAGGACCTCCTCGACT
                     CAGTCAAGAGCTTCGAGAACTACATCAAGGCCTTCTTCGGCGAGGGCAAGGAGACAAA
                     CCGCGACGAGAGCTTCTACGGCGACTTCGTCCTCGCCTATGACATCCTCCTCAAGGTG
                     GACCACATCTACGACGCCATCAGGAACTACGTGACCCAGAAGCCCTACTCAAAGGACA
                     AGTTCAAGCTCTACTTCCAGAACCCCCAGTTCATGGGCGGCTGGGACAAGGACAAGGA
                     GACTGACTACCGCGCCACCATCCTCCGCTACGGCTCAAAGTACTACCTCGCCATCATG
                     GACAAGAAGTACGCCAAGTGCCTCCAGAAGATCGACAAGGATGATGTGAACGGCAACT
                     ACGAGAAGATCAACTACAAGCTCCTCCCCGGCCCCAACAAGATGCTACCCAAGGTCTT
                     CTTCTCCAAGAAGTGGATGGCCTACTACAACCCCTCCGAGGACATCCAGAAGATCTAC
                     AAGAACGGCACCTTCAAGAAGGGTGACATGTTCAACCTCAACGACTGCCACAAGCTGA
                     TCGACTTCTTCAAGGACAGCATCTCAAGGTACCCCAAGTGGAGCAACGCCTACGACTT
                     CAACTTCTCTGAGACGGAGAAGTACAAGGACATCGCCGGCTTCTACCGGGAGGTGGAG
                     GAGCAAGGCTACAAGGTGAGCTTCGAGTCCGCCTCCAAGAAGGAGGTGGACAAGCTGG
                     TGGAGGAGGGCAAGCTCTACATGTTCCAGATCTACAACAAGGACTTCTCTGACAAGAG
                     CCACGGCACCCCCAACCTACACACCATGTACTTCAAGCTCCTCTTCGACGAGAACAAC
                     CACGGCCAGATCCGCCTCTCCGGCGGCGCCGAGCTCTTCATGAGGAGGGCCTCCCTCA
                     AGAAGGAGGAGCTGGTGGTTCACCCGGCCAACAGCCCCATCGCCAACAAGAACCCTGA
                     CAACCCCAAGAAGACCACCACCCTCTCCTACGACGTCTACAAGGACAAGAGGTTCTCA
                     GAAGACCAGTACGAGCTCCACATCCCCATCGCCATCAACAAGTGCCCCAAGAACATCT
                     TCAAGATCAACACCGAGGTGAGGGTGCTACTCAAGCATGATGATAACCCCTACGTCAT
                     CGGCATCGCCCGCGGCGAGAGGAACCTCCTCTACATCGTGGTGGTGGACGGCAAGGGC
                     AACATCGTGGAGCAGTACTCCTTGAACGAGATCATCAACAACTTCAACGGCATCAGGA
                     TCAAGACCGACTACCACTCACTCCTTGACAAGAAGGAGAAGGAGAGGTTCGAGGCCCG
                     GCAAAATTGGACAAGCATCGAGAACATCAAGGAGCTGAAGGCCGGCTACATCTCCCAG
                     GTGGTGCACAAGATCTGTGAGCTGGTGGAGAAGTACGACGCCGTCATCGCCCTGGAGG
                     ACCTCAACAGCGGCTTCAAGAACTCAAGGGTCAAGGTGGAGAAGCAGGTGTACCAGAA
                     GTTCGAGAAGATGCTCATCGACAAGCTGAACTACATGGTGGACAAGAAGAGCAACCCC
                     TGCGCCACCGGCGGCGCCCTCAAGGGCTACCAGATCACCAACAAGTTTGAGAGCTTCA
                     AGAGCATGTCAACCCAGAACGGCTTCATCTTCTACATCCCGGCCTGGCTCACCTCCAA
                     GATCGACCCCTCCACCGGCTTCGTCAACCTCCTCAAGACCAAGTACACCTCCATAGCC
                     GACAGCAAGAAGTTCATCTCCTCCTTCGACCGCATCATGTACGTCCCTGAGGAGGACC
                     TCTTCGAGTTCGCCCTCGACTACAAGAACTTCTCAAGGACCGACGCCGACTACATCAA
                     GAAGTGGAAGCTCTACAGCTACGGCAACCGCATCAGGATCTTCAGGAACCCCAAGAAG
                     AACAACGTCTTCGACTGGGAGGAGGTGTGCCTGACCTCCGCCTACAAGGAGCTCTTCA
                     ACAAGTACGGCATCAACTACCAGCAAGGTGACATCAGGGCGCTCCTATGCGAGCAGAG
                     CGACAAGGCCTTCTACTCCTCCTTCATGGCCCTCATGAGCTTGATGCTCCAGATGAGG
                     AACAGCATCACCGGCCGCACCGACGTCGACTTCCTCATCTCCCCCGTCAAGAACTCTG
                     ATGGCATCTTCTACGACTCAAGGAACTACGAGGCCCAGGAGAACGCCATCCTCCCCAA
                     GAACGCCGACGCCAACGGCGCCTACAACATCGCCAGGAAGGTGCTCTGGGCCATCGGC
                     CAGTTCAAGAAGGCCGAGGATGAGAAGCTGGACAAGGTGAAGATCGCCATCTCAAACA
                     AGGAGTGGCTGGAGTACGCCCAGACCTCCGTCAAGCAC"
                     /X-Transferred_From="TaLbCas12a"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:oc-ecpnky2"">TaLbCas12a</a>"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:qo-ecpph81"">TaLbCas12a(D832A)</a>"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TaLbCas12a(D832A)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:19-ecu990z"">TaLbCas12a(D832A)</a>"
                     /label="TaLbCas12a(D832A)"
     NLS             6359..6409
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     CDS             6410..6466
                     /created_by="User"
                     /Transferred_From="P2A"
                     /Transferred_Similarity="100.00%"
                     /label="P2A"
     CDS             6467..7183
                     /note="A206K"
                     /codon_start=1
                     /transl_table=11
                     /product="GFP"
                     /protein_id="AHE38511.1"
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="GFP"
                     /Transferred_Similarity="100.00%"
                     /label="GFP"
     Linker          7187..7234
                     /created_by="wacae"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             7235..7483
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      7491..7762
                     /label="G7T"
     Linker          7767..7815
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(7852..7872)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        7933..7983
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    7984..8793
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      8940..9528
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          10127..10147
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta
       61 taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt
      121 atacatatat ttaaacttta ctctacgaat aatataatct atagtactac aataatatca
      181 gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt
      241 ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg
      301 caaatagctt cacctatata atacttcatc cattttatta gtacatccat ttagggttta
      361 gggttaatgg tttttataga ctaatttttt tagtacatct attttattct attttagcct
      421 ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa
      481 tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta
      541 aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt
      601 ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca
      661 cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg
      721 ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag
      781 gcggcctcct cctcctctca cggcacggca gctacggggg attcctttcc caccgctcct
      841 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
      901 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
      961 ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc taccttctct
     1021 agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc atgtttgtgt
     1081 tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac
     1141 gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc
     1201 tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt
     1261 tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt
     1321 tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc
     1381 ggagtagaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg
     1441 tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata
     1501 ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg
     1561 gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac
     1621 tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct
     1681 tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat
     1741 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     1801 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     1861 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     1921 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     1981 tctgcaaaca atgaaaagaa ccgccgacgg cagcgaattc gagcctaaga agaagaggaa
     2041 ggtttccggc ggcagctcag gaggttcctc tgaggtggag ttttcccacg agtactggat
     2101 gagacatgcc ctgaccctgg ccaagagggc acgggatgag agggaggtgc ctgtgggagc
     2161 cgtgctggtg ctgaacaata gagtgatcgg cgagggctgg aacagagcca tcggcctgca
     2221 cgacccaaca gcccatgccg aaattatggc cctgagacag ggcggcctgg tcatgcagaa
     2281 ctacagactg attgacgcca ccctgtacgt gacattcgag ccttgcgtga tgtgcgccgg
     2341 cgccatgatc cactctagga tcggccgcgt ggtgtttggc gtgaggaact caaaaagagg
     2401 cgccgcaggc tccctgatga acgtgctgaa ctaccccggc atgaatcacc gcgtcgaaat
     2461 taccgaggga atcctggcag atgaatgtgc cgccctgctg tgcgatttct atcggatgcc
     2521 tagacaggtg ttcaatgctc agaagaaggc ccagagctcc atcaactccg gaggatctag
     2581 cggaggctcc tctggctctg agacacctgg cacaagcgag agcgcaacac ctgaaagcag
     2641 cgggggcagc agcggggggt caggctccat gagcaagctg gagaagttca ccaactgcta
     2701 ctcactctcc aagaccctcc gcttcaaggc catccccgtc ggcaagaccc aggagaacat
     2761 cgacaacaag aggctgctgg tggaggatga gaagagggcc gaggactaca agggcgtcaa
     2821 gaagctcctc gaccggtact acctctcatt catcaatgat gtcctccact ccatcaagct
     2881 gaagaacctc aacaactaca tctccctctt ccgcaagaag acaaggaccg agaaggagaa
     2941 caaggagctg gagaacctgg agatcaacct ccgcaaggag atcgccaagg ccttcaaggg
     3001 caacgagggc tacaagagcc tcttcaagaa ggacatcatc gagacgatcc taccggagtt
     3061 cctggatgac aaggacgaga tcgccctggt gaacagcttc aacggcttca ccaccgcctt
     3121 caccggcttc ttcgacaacc gggagaacat gttctccgag gaggccaaga gcacctccat
     3181 cgccttcagg tgcatcaacg agaacctcac ccgctacatc tcaaacatgg acatcttcga
     3241 gaaggtggac gccatcttcg acaagcatga ggtccaggag atcaaggaga agatcctgaa
     3301 ctccgactac gacgtggagg acttcttcga gggcgagttc ttcaacttcg tcctgaccca
     3361 agaaggcatc gacgtctaca acgccatcat cggcggcttc gtcaccgagt caggtgagaa
     3421 gatcaagggc ctcaacgagt acatcaacct ctacaaccag aagaccaagc agaagctccc
     3481 caagttcaag cccctctaca agcaggtgct ctccgaccgt gagagcttga gcttctacgg
     3541 cgagggctac acctccgacg aggaggtgct ggaggtcttc cgcaacaccc tcaacaagaa
     3601 ctcagagatc ttctcctcca tcaagaagct ggagaagctc ttcaagaact tcgacgagta
     3661 ctcctccgcc ggcatcttcg tgaagaacgg cccggccatc tcaaccatct ccaaggacat
     3721 cttcggcgag tggaacgtca tccgtgacaa gtggaacgcc gagtacgacg acatccacct
     3781 caagaagaag gccgtggtga ccgagaagta cgaggacgac cgccgcaaga gcttcaagaa
     3841 gatcggcagc ttctcactgg agcagctcca ggagtacgcc gacgccgacc tctccgtggt
     3901 ggagaagctg aaggagatca tcatccagaa ggtggacgag atctacaagg tgtacggcag
     3961 ctctgagaag ctcttcgacg ccgacttcgt cctggagaag agcctcaaga agaacgacgc
     4021 cgtggtggcc atcatgaagg acctcctcga ctcagtcaag agcttcgaga actacatcaa
     4081 ggccttcttc ggcgagggca aggagacaaa ccgcgacgag agcttctacg gcgacttcgt
     4141 cctcgcctat gacatcctcc tcaaggtgga ccacatctac gacgccatca ggaactacgt
     4201 gacccagaag ccctactcaa aggacaagtt caagctctac ttccagaacc cccagttcat
     4261 gggcggctgg gacaaggaca aggagactga ctaccgcgcc accatcctcc gctacggctc
     4321 aaagtactac ctcgccatca tggacaagaa gtacgccaag tgcctccaga agatcgacaa
     4381 ggatgatgtg aacggcaact acgagaagat caactacaag ctcctccccg gccccaacaa
     4441 gatgctaccc aaggtcttct tctccaagaa gtggatggcc tactacaacc cctccgagga
     4501 catccagaag atctacaaga acggcacctt caagaagggt gacatgttca acctcaacga
     4561 ctgccacaag ctgatcgact tcttcaagga cagcatctca aggtacccca agtggagcaa
     4621 cgcctacgac ttcaacttct ctgagacgga gaagtacaag gacatcgccg gcttctaccg
     4681 ggaggtggag gagcaaggct acaaggtgag cttcgagtcc gcctccaaga aggaggtgga
     4741 caagctggtg gaggagggca agctctacat gttccagatc tacaacaagg acttctctga
     4801 caagagccac ggcaccccca acctacacac catgtacttc aagctcctct tcgacgagaa
     4861 caaccacggc cagatccgcc tctccggcgg cgccgagctc ttcatgagga gggcctccct
     4921 caagaaggag gagctggtgg ttcacccggc caacagcccc atcgccaaca agaaccctga
     4981 caaccccaag aagaccacca ccctctccta cgacgtctac aaggacaaga ggttctcaga
     5041 agaccagtac gagctccaca tccccatcgc catcaacaag tgccccaaga acatcttcaa
     5101 gatcaacacc gaggtgaggg tgctactcaa gcatgatgat aacccctacg tcatcggcat
     5161 cgcccgcggc gagaggaacc tcctctacat cgtggtggtg gacggcaagg gcaacatcgt
     5221 ggagcagtac tccttgaacg agatcatcaa caacttcaac ggcatcagga tcaagaccga
     5281 ctaccactca ctccttgaca agaaggagaa ggagaggttc gaggcccggc aaaattggac
     5341 aagcatcgag aacatcaagg agctgaaggc cggctacatc tcccaggtgg tgcacaagat
     5401 ctgtgagctg gtggagaagt acgacgccgt catcgccctg gaggacctca acagcggctt
     5461 caagaactca agggtcaagg tggagaagca ggtgtaccag aagttcgaga agatgctcat
     5521 cgacaagctg aactacatgg tggacaagaa gagcaacccc tgcgccaccg gcggcgccct
     5581 caagggctac cagatcacca acaagtttga gagcttcaag agcatgtcaa cccagaacgg
     5641 cttcatcttc tacatcccgg cctggctcac ctccaagatc gacccctcca ccggcttcgt
     5701 caacctcctc aagaccaagt acacctccat agccgacagc aagaagttca tctcctcctt
     5761 cgaccgcatc atgtacgtcc ctgaggagga cctcttcgag ttcgccctcg actacaagaa
     5821 cttctcaagg accgacgccg actacatcaa gaagtggaag ctctacagct acggcaaccg
     5881 catcaggatc ttcaggaacc ccaagaagaa caacgtcttc gactgggagg aggtgtgcct
     5941 gacctccgcc tacaaggagc tcttcaacaa gtacggcatc aactaccagc aaggtgacat
     6001 cagggcgctc ctatgcgagc agagcgacaa ggccttctac tcctccttca tggccctcat
     6061 gagcttgatg ctccagatga ggaacagcat caccggccgc accgacgtcg acttcctcat
     6121 ctcccccgtc aagaactctg atggcatctt ctacgactca aggaactacg aggcccagga
     6181 gaacgccatc ctccccaaga acgccgacgc caacggcgcc tacaacatcg ccaggaaggt
     6241 gctctgggcc atcggccagt tcaagaaggc cgaggatgag aagctggaca aggtgaagat
     6301 cgccatctca aacaaggagt ggctggagta cgcccagacc tccgtcaagc actcaggtaa
     6361 aagaaccgcc gacggcagcg aattcgagcc taagaagaag aggaaggttg ctactaattt
     6421 ttctcttctt aagcaagctg gagatgttga agaaaatcct ggacccatgg tgagcaaggg
     6481 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     6541 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     6601 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     6661 cacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     6721 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     6781 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     6841 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     6901 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     6961 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     7021 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     7081 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     7141 gaccgccgcc gggatcactc acggcatgga cgagctgtac aagtcaggtt ctggttcagg
     7201 ttcaggttct ggatctggaa gtggatctgg atcagcggcc gcatctgaat tcaagcgtga
     7261 agagcaagca aggaaagcta aggtgaacaa tgagaaaaag acggaaatag tgaaaccaga
     7321 gagttgtagc aatgaaggag atgtcaagga tctgaaaaga aaggactctg aggatggaaa
     7381 cgagggtgag gaagaagaag cttcttcgaa accgaaaaag ccaaaagttg ctctttctca
     7441 tcttcaggac attgacgaca cagaagctga tcaagaagaa gagtaactgc ccccgatgag
     7501 ctaagctagc tatatcatca atttatgtat tacacataat atcgcactca gtctttcatc
     7561 tacggcaatg taccagctga tataatcagt tattgaaata tttctgaatt taaacttgca
     7621 tcaataaatt tatgtttttg cttggactat aatacctgac ttgttatttt atcaataaat
     7681 atttaaacta tatttctttc aagatgggaa ttaacatcta caaattgcct tttcttatcg
     7741 accatgtact cgacggccga gtactataag ctaggtagta actagtgcga ctgtctagaa
     7801 tcgcgtatgc gagtgtatga gctcgatatc ccctatagtg agtcgtatta catggtcata
     7861 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     7921 aaaataatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     7981 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     8041 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     8101 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     8161 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     8221 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     8281 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     8341 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     8401 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     8461 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     8521 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     8581 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga
     8641 atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct
     8701 tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg
     8761 cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac
     8821 tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac
     8881 gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9121 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9601 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     9661 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     9721 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     9781 cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa
     9841 gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc
     9901 tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca
     9961 aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
    10021 aacgaaaggc ccagacttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct
    10081 actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa aacgacggcc
    10141 agtcttaagc tcgggcccac ct
//
