LOCUS       pGG-B-BP-TadA9-32aa-C        3358 bp    DNA     circular UNA 23-FEB-2023
DEFINITION  .
ACCESSION   urn.local...bl-en4mbd4
VERSION     urn.local...bl-en4mbd4
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2h-c4nnd55"">B</a>"
                     /label="B"
     NLS             15..65
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     CDS             66..89
                     /created_by="chgai"
                     /label="2xSGGS"
     CDS             90..587
                     /standard_name="TadAmax10 (ABE8)"
                     /modified_by="thjac"
                     /label="TadA9"
     Linker          588..683
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="32-aa linker"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a3-dgycryz"">32-aa linker</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24;
                     XTEN (<a href=""urn:local:.:a3-dgycryz"">32-aa
                     linker</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24;
                     <a href=""urn:local:.:a5-dgycryz"">XTEN</a>"
                     /label="32-aa linker"
     GG              complement(684..694)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:294-ciz70s8"">C</a>"
                     /label="C"
     primer          complement(714..734)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1214..1318
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1319..2179
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2350..2938
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3302..3323
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaaac aatgaaaaga accgccgacg gcagcgaatt cgagcctaag aagaagagga
       61 aggtttccgg cggcagctca ggaggttcct ctgaggtgga gttttcccac gagtactgga
      121 tgagacatgc cctgaccctg gccaagaggg cacgggatga gagggaggtg cctgtgggag
      181 ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc atcggcctgc
      241 acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg gtcatgcaga
      301 actacagact gattgacgcc accctgtact cgacattcga gccttgcgtg atgtgcgccg
      361 gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac tcaaaaagag
      421 gcgccgcagg ctccctgatg aacgtgctga actaccccgg catgaatcac cgcgtcgaaa
      481 ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgcgatttc tatcggatgc
      541 ctagacgggt gttcaatgct cagaagaagg cccagagctc catcaactcc ggaggatcta
      601 gcggaggctc ctctggctct gagacacctg gcacaagcga gagcgcaaca cctgaaagca
      661 gcgggggcag cagcgggggg tcaggcttga gaccgaattc tcgccctata gtgagtcgta
      721 ttacctggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
      781 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
      841 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
      901 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
      961 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     1021 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     1081 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     1141 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1201 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1261 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1321 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1381 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1441 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1501 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1561 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1621 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1681 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1741 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1801 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1861 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1921 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1981 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg
     2041 aggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2101 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2161 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2221 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2281 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2341 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2401 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2461 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2521 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2581 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2641 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2701 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2761 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2821 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2881 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2941 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3001 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3061 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     3181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     3241 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     3301 tgtgagcgga taacaatttc acacgagcta tttaggtgac actatagaag tgaagctt
//
