LOCUS       pZmUBI-BP-TadA8e-TaLbCas12a(D832A)-3xSV40-NLS-CaMVT       11819 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...b5-en4mbcw
VERSION     urn.local...b5-en4mbcw
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..864
                     /regulatory_class="promoter"
                     /gene="CaMV 35S"
                     /note="internal BsaI recognition site removed"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:6v-dgycryx"">pCaMV 35S</a>)"
                     /label="p35S"
     CDS             899..1606
                     /created_by="User"
                     /modified_by="User"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:3a-c4nnd56"">mCherry</a>"
                     /label="mCherry"
     Linker          1610..1657
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker
                     (<a href=""urn:local:thjac:1g-c4nnd54"">(GS)8</a>)"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             1658..1906
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      1914..2181
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /label="NOST"
     promoter        2210..4195
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/1;
                     ZmUBIL.2 (<a
                     href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     intron          3187..4196
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     NLS             4203..4253
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS BP*"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; BP* (<a
                     href=""urn:local:.:a1-dgycryz"">NLS BP*</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:30/0;
                     SV40 (<a href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /Alternative_Match="94.44%; Adjusted-Interval-0:0/0; BP
                     (<a href=""urn:local:.:a0-dgycryz"">NLS BP</a>)"
                     /label="BP*"
     CDS             4254..4277
                     /created_by="chgai"
                     /label="2xSGGS"
     CDS             4278..4775
                     /standard_name="TadAmax10 (ABE8)"
                     /label="TadAmax10 (ABE8)"
     Linker          4776..4871
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="32-aa linker"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a3-dgycryz"">32-aa linker</a>"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24;
                     XTEN (<a href=""urn:local:.:a3-dgycryz"">32-aa
                     linker</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:24/-24;
                     <a href=""urn:local:.:a5-dgycryz"">XTEN</a>"
                     /label="32-aa linker"
     CDS             4878..8561
                     /%_Identity="99.9457111834962"
                     /Motif="ATGAGCAAGCTGGAGAAGTTCACCAACTGCTACTCACTCTCCAAGACCCT
                     CCGCTTCAAGGCCATCCCCGTCGGCAAGACCCAGGAGAACATCGACAACAAGAGGCTG
                     CTGGTGGAGGATGAGAAGAGGGCCGAGGACTACAAGGGCGTCAAGAAGCTCCTCGACC
                     GGTACTACCTCTCATTCATCAATGATGTCCTCCACTCCATCAAGCTGAAGAACCTCAA
                     CAACTACATCTCCCTCTTCCGCAAGAAGACAAGGACCGAGAAGGAGAACAAGGAGCTG
                     GAGAACCTGGAGATCAACCTCCGCAAGGAGATCGCCAAGGCCTTCAAGGGCAACGAGG
                     GCTACAAGAGCCTCTTCAAGAAGGACATCATCGAGACGATCCTACCGGAGTTCCTGGA
                     TGACAAGGACGAGATCGCCCTGGTGAACAGCTTCAACGGCTTCACCACCGCCTTCACC
                     GGCTTCTTCCGCAACCGGGAGAACATGTTCTCCGAGGAGGCCAAGAGCACCTCCATCG
                     CCTTCAGGTGCATCAACGAGAACCTCACCCGCTACATCTCAAACATGGACATCTTCGA
                     GAAGGTGGACGCCATCTTCGACAAGCATGAGGTCCAGGAGATCAAGGAGAAGATCCTG
                     AACTCCGACTACGACGTGGAGGACTTCTTCGAGGGCGAGTTCTTCAACTTCGTCCTGA
                     CCCAAGAAGGCATCGACGTCTACAACGCCATCATCGGCGGCTTCGTCACCGAGTCAGG
                     TGAGAAGATCAAGGGCCTCAACGAGTACATCAACCTCTACAACCAGAAGACCAAGCAG
                     AAGCTCCCCAAGTTCAAGCCCCTCTACAAGCAGGTGCTCTCCGACCGTGAGAGCTTGA
                     GCTTCTACGGCGAGGGCTACACCTCCGACGAGGAGGTGCTGGAGGTCTTCCGCAACAC
                     CCTCAACAAGAACTCAGAGATCTTCTCCTCCATCAAGAAGCTGGAGAAGCTCTTCAAG
                     AACTTCGACGAGTACTCCTCCGCCGGCATCTTCGTGAAGAACGGCCCGGCCATCTCAA
                     CCATCTCCAAGGACATCTTCGGCGAGTGGAACGTCATCCGTGACAAGTGGAACGCCGA
                     GTACGACGACATCCACCTCAAGAAGAAGGCCGTGGTGACCGAGAAGTACGAGGACGAC
                     CGCCGCAAGAGCTTCAAGAAGATCGGCAGCTTCTCACTGGAGCAGCTCCAGGAGTACG
                     CCGACGCCGACCTCTCCGTGGTGGAGAAGCTGAAGGAGATCATCATCCAGAAGGTGGA
                     CGAGATCTACAAGGTGTACGGCAGCTCTGAGAAGCTCTTCGACGCCGACTTCGTCCTG
                     GAGAAGAGCCTCAAGAAGAACGACGCCGTGGTGGCCATCATGAAGGACCTCCTCGACT
                     CAGTCAAGAGCTTCGAGAACTACATCAAGGCCTTCTTCGGCGAGGGCAAGGAGACAAA
                     CCGCGACGAGAGCTTCTACGGCGACTTCGTCCTCGCCTATGACATCCTCCTCAAGGTG
                     GACCACATCTACGACGCCATCAGGAACTACGTGACCCAGAAGCCCTACTCAAAGGACA
                     AGTTCAAGCTCTACTTCCAGAACCCCCAGTTCATGGGCGGCTGGGACAAGGACAAGGA
                     GACTGACTACCGCGCCACCATCCTCCGCTACGGCTCAAAGTACTACCTCGCCATCATG
                     GACAAGAAGTACGCCAAGTGCCTCCAGAAGATCGACAAGGATGATGTGAACGGCAACT
                     ACGAGAAGATCAACTACAAGCTCCTCCCCGGCCCCAACAAGATGCTACCCAAGGTCTT
                     CTTCTCCAAGAAGTGGATGGCCTACTACAACCCCTCCGAGGACATCCAGAAGATCTAC
                     AAGAACGGCACCTTCAAGAAGGGTGACATGTTCAACCTCAACGACTGCCACAAGCTGA
                     TCGACTTCTTCAAGGACAGCATCTCAAGGTACCCCAAGTGGAGCAACGCCTACGACTT
                     CAACTTCTCTGAGACGGAGAAGTACAAGGACATCGCCGGCTTCTACCGGGAGGTGGAG
                     GAGCAAGGCTACAAGGTGAGCTTCGAGTCCGCCTCCAAGAAGGAGGTGGACAAGCTGG
                     TGGAGGAGGGCAAGCTCTACATGTTCCAGATCTACAACAAGGACTTCTCTGACAAGAG
                     CCACGGCACCCCCAACCTACACACCATGTACTTCAAGCTCCTCTTCGACGAGAACAAC
                     CACGGCCAGATCCGCCTCTCCGGCGGCGCCGAGCTCTTCATGAGGAGGGCCTCCCTCA
                     AGAAGGAGGAGCTGGTGGTTCACCCGGCCAACAGCCCCATCGCCAACAAGAACCCTGA
                     CAACCCCAAGAAGACCACCACCCTCTCCTACGACGTCTACAAGGACAAGAGGTTCTCA
                     GAAGACCAGTACGAGCTCCACATCCCCATCGCCATCAACAAGTGCCCCAAGAACATCT
                     TCAAGATCAACACCGAGGTGAGGGTGCTACTCAAGCATGATGATAACCCCTACGTCAT
                     CGGCATCGCCCGCGGCGAGAGGAACCTCCTCTACATCGTGGTGGTGGACGGCAAGGGC
                     AACATCGTGGAGCAGTACTCCTTGAACGAGATCATCAACAACTTCAACGGCATCAGGA
                     TCAAGACCGACTACCACTCACTCCTTGACAAGAAGGAGAAGGAGAGGTTCGAGGCCCG
                     GCAAAATTGGACAAGCATCGAGAACATCAAGGAGCTGAAGGCCGGCTACATCTCCCAG
                     GTGGTGCACAAGATCTGTGAGCTGGTGGAGAAGTACGACGCCGTCATCGCCCTGGAGG
                     ACCTCAACAGCGGCTTCAAGAACTCAAGGGTCAAGGTGGAGAAGCAGGTGTACCAGAA
                     GTTCGAGAAGATGCTCATCGACAAGCTGAACTACATGGTGGACAAGAAGAGCAACCCC
                     TGCGCCACCGGCGGCGCCCTCAAGGGCTACCAGATCACCAACAAGTTTGAGAGCTTCA
                     AGAGCATGTCAACCCAGAACGGCTTCATCTTCTACATCCCGGCCTGGCTCACCTCCAA
                     GATCGACCCCTCCACCGGCTTCGTCAACCTCCTCAAGACCAAGTACACCTCCATAGCC
                     GACAGCAAGAAGTTCATCTCCTCCTTCGACCGCATCATGTACGTCCCTGAGGAGGACC
                     TCTTCGAGTTCGCCCTCGACTACAAGAACTTCTCAAGGACCGACGCCGACTACATCAA
                     GAAGTGGAAGCTCTACAGCTACGGCAACCGCATCAGGATCTTCAGGAACCCCAAGAAG
                     AACAACGTCTTCGACTGGGAGGAGGTGTGCCTGACCTCCGCCTACAAGGAGCTCTTCA
                     ACAAGTACGGCATCAACTACCAGCAAGGTGACATCAGGGCGCTCCTATGCGAGCAGAG
                     CGACAAGGCCTTCTACTCCTCCTTCATGGCCCTCATGAGCTTGATGCTCCAGATGAGG
                     AACAGCATCACCGGCCGCACCGACGTCGACTTCCTCATCTCCCCCGTCAAGAACTCTG
                     ATGGCATCTTCTACGACTCAAGGAACTACGAGGCCCAGGAGAACGCCATCCTCCCCAA
                     GAACGCCGACGCCAACGGCGCCTACAACATCGCCAGGAAGGTGCTCTGGGCCATCGGC
                     CAGTTCAAGAAGGCCGAGGATGAGAAGCTGGACAAGGTGAAGATCGCCATCTCAAACA
                     AGGAGTGGCTGGAGTACGCCCAGACCTCCGTCAAGCAC"
                     /X-Transferred_From="TaLbCas12a"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:oc-ecpnky2"">TaLbCas12a</a>"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:qo-ecpph81"">TaLbCas12a(D832A)</a>"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TaLbCas12a(D832A)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:19-ecu990z"">TaLbCas12a(D832A)</a>"
                     /label="TaLbCas12a(D832A)"
     NLS             8568..8654
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3xSV40"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:5q-ecucp4e"">3xSV40</a>"
                     /modified_by="mamee"
                     /label="3xSV40"
     terminator      8664..9395
                     /created_by="mamee"
                     /label="35ST"
     terminator      8716..8892
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /label="CaMV poly(A) signal"
     Linker          9400..9448
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(9509..9529)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        9590..9640
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    9641..10450
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      10597..11185
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          11784..11804
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ccgatccatt cggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
       61 tggttagaga ggcttacgca gcacgtctca tcaagacgat ctacccgagc aataatctcc
      121 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
      181 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
      241 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
      301 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
      361 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
      421 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
      481 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
      541 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
      601 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
      661 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      721 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      781 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      841 tggagagaac acgggggacg agctaacagt attcagtcga ctggtaccaa caggctccat
      901 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      961 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1021 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1081 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1141 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1201 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     1261 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1321 gcagaagaag acgatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1381 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1441 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1501 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     1561 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtcag gttctggttc
     1621 aggttcaggt tctggatctg gaagtggatc tggatcagcg gccgcatctg aattcaagcg
     1681 tgaagagcaa gcaaggaaag ctaaggtgaa caatgagaaa aagacggaaa tagtgaaacc
     1741 agagagttgt agcaatgaag gagatgtcaa ggatctgaaa agaaaggact ctgaggatgg
     1801 aaacgagggt gaggaagaag aagcttcttc gaaaccgaaa aagccaaaag ttgctctttc
     1861 tcatcttcag gacattgacg acacagaagc tgatcaagaa gaagagtaac tgcctagagt
     1921 caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     1981 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     2041 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     2101 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     2161 tcatctatgt tactagatcg aactataagc taggtagtaa ctagtacctg tgcagcgtga
     2221 cccggtcgtg cccctctcta gagataatga gcattgcatg tctaagttat aaaaaattac
     2281 cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta tacatatatt
     2341 taaactttac tctacgaata atataatcta tagtactaca ataatatcag tgttttagag
     2401 aatcatataa atgaacagtt agacatggtc taaaggacaa ttgagtattt tgacaacagg
     2461 actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc aaatagcttc
     2521 acctatataa tacttcatcc attttattag tacatccatt tagggtttag ggttaatggt
     2581 ttttatagac taattttttt agtacatcta ttttattcta ttttagcctc taaattaaga
     2641 aaactaaaac tctattttag tttttttatt taataattta gatataaaat agaataaaat
     2701 aaagtgacta aaaattaaac aaataccctt taagaaatta aaaaaactaa ggaaacattt
     2761 ttcttgtttc gagtagataa tgccagcctg ttaaacgccg tcgacgagtc taacggacac
     2821 caaccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg
     2881 tcgctgcctc tggacccctc tcgagagttc cgctccaccg ttggacttgc tccgctgtcg
     2941 gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg cggcctcctc
     3001 ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct
     3061 tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt
     3121 gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc
     3181 ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt
     3241 ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt
     3301 ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt
     3361 tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc
     3421 cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct
     3481 tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt
     3541 tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt
     3601 ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata
     3661 ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt
     3721 tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat
     3781 gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact
     3841 acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg
     3901 agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac
     3961 tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct
     4021 atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat
     4081 ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg
     4141 cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt ctgcaaacaa
     4201 tgaaaagaac cgccgacggc agcgaattcg agcctaagaa gaagaggaag gtttccggcg
     4261 gcagctcagg aggttcctct gaggtggagt tttcccacga gtactggatg agacatgccc
     4321 tgaccctggc caagagggca cgggatgaga gggaggtgcc tgtgggagcc gtgctggtgc
     4381 tgaacaatag agtgatcggc gagggctgga acagagccat cggcctgcac gacccaacag
     4441 cccatgccga aattatggcc ctgagacagg gcggcctggt catgcagaac tacagactga
     4501 ttgacgccac cctgtacgtg acattcgagc cttgcgtgat gtgcgccggc gccatgatcc
     4561 actctaggat cggccgcgtg gtgtttggcg tgaggaactc aaaaagaggc gccgcaggct
     4621 ccctgatgaa cgtgctgaac taccccggca tgaatcaccg cgtcgaaatt accgagggaa
     4681 tcctggcaga tgaatgtgcc gccctgctgt gcgatttcta tcggatgcct agacaggtgt
     4741 tcaatgctca gaagaaggcc cagagctcca tcaactccgg aggatctagc ggaggctcct
     4801 ctggctctga gacacctggc acaagcgaga gcgcaacacc tgaaagcagc gggggcagca
     4861 gcggggggtc aggctccatg agcaagctgg agaagttcac caactgctac tcactctcca
     4921 agaccctccg cttcaaggcc atccccgtcg gcaagaccca ggagaacatc gacaacaaga
     4981 ggctgctggt ggaggatgag aagagggccg aggactacaa gggcgtcaag aagctcctcg
     5041 accggtacta cctctcattc atcaatgatg tcctccactc catcaagctg aagaacctca
     5101 acaactacat ctccctcttc cgcaagaaga caaggaccga gaaggagaac aaggagctgg
     5161 agaacctgga gatcaacctc cgcaaggaga tcgccaaggc cttcaagggc aacgagggct
     5221 acaagagcct cttcaagaag gacatcatcg agacgatcct accggagttc ctggatgaca
     5281 aggacgagat cgccctggtg aacagcttca acggcttcac caccgccttc accggcttct
     5341 tcgacaaccg ggagaacatg ttctccgagg aggccaagag cacctccatc gccttcaggt
     5401 gcatcaacga gaacctcacc cgctacatct caaacatgga catcttcgag aaggtggacg
     5461 ccatcttcga caagcatgag gtccaggaga tcaaggagaa gatcctgaac tccgactacg
     5521 acgtggagga cttcttcgag ggcgagttct tcaacttcgt cctgacccaa gaaggcatcg
     5581 acgtctacaa cgccatcatc ggcggcttcg tcaccgagtc aggtgagaag atcaagggcc
     5641 tcaacgagta catcaacctc tacaaccaga agaccaagca gaagctcccc aagttcaagc
     5701 ccctctacaa gcaggtgctc tccgaccgtg agagcttgag cttctacggc gagggctaca
     5761 cctccgacga ggaggtgctg gaggtcttcc gcaacaccct caacaagaac tcagagatct
     5821 tctcctccat caagaagctg gagaagctct tcaagaactt cgacgagtac tcctccgccg
     5881 gcatcttcgt gaagaacggc ccggccatct caaccatctc caaggacatc ttcggcgagt
     5941 ggaacgtcat ccgtgacaag tggaacgccg agtacgacga catccacctc aagaagaagg
     6001 ccgtggtgac cgagaagtac gaggacgacc gccgcaagag cttcaagaag atcggcagct
     6061 tctcactgga gcagctccag gagtacgccg acgccgacct ctccgtggtg gagaagctga
     6121 aggagatcat catccagaag gtggacgaga tctacaaggt gtacggcagc tctgagaagc
     6181 tcttcgacgc cgacttcgtc ctggagaaga gcctcaagaa gaacgacgcc gtggtggcca
     6241 tcatgaagga cctcctcgac tcagtcaaga gcttcgagaa ctacatcaag gccttcttcg
     6301 gcgagggcaa ggagacaaac cgcgacgaga gcttctacgg cgacttcgtc ctcgcctatg
     6361 acatcctcct caaggtggac cacatctacg acgccatcag gaactacgtg acccagaagc
     6421 cctactcaaa ggacaagttc aagctctact tccagaaccc ccagttcatg ggcggctggg
     6481 acaaggacaa ggagactgac taccgcgcca ccatcctccg ctacggctca aagtactacc
     6541 tcgccatcat ggacaagaag tacgccaagt gcctccagaa gatcgacaag gatgatgtga
     6601 acggcaacta cgagaagatc aactacaagc tcctccccgg ccccaacaag atgctaccca
     6661 aggtcttctt ctccaagaag tggatggcct actacaaccc ctccgaggac atccagaaga
     6721 tctacaagaa cggcaccttc aagaagggtg acatgttcaa cctcaacgac tgccacaagc
     6781 tgatcgactt cttcaaggac agcatctcaa ggtaccccaa gtggagcaac gcctacgact
     6841 tcaacttctc tgagacggag aagtacaagg acatcgccgg cttctaccgg gaggtggagg
     6901 agcaaggcta caaggtgagc ttcgagtccg cctccaagaa ggaggtggac aagctggtgg
     6961 aggagggcaa gctctacatg ttccagatct acaacaagga cttctctgac aagagccacg
     7021 gcacccccaa cctacacacc atgtacttca agctcctctt cgacgagaac aaccacggcc
     7081 agatccgcct ctccggcggc gccgagctct tcatgaggag ggcctccctc aagaaggagg
     7141 agctggtggt tcacccggcc aacagcccca tcgccaacaa gaaccctgac aaccccaaga
     7201 agaccaccac cctctcctac gacgtctaca aggacaagag gttctcagaa gaccagtacg
     7261 agctccacat ccccatcgcc atcaacaagt gccccaagaa catcttcaag atcaacaccg
     7321 aggtgagggt gctactcaag catgatgata acccctacgt catcggcatc gcccgcggcg
     7381 agaggaacct cctctacatc gtggtggtgg acggcaaggg caacatcgtg gagcagtact
     7441 ccttgaacga gatcatcaac aacttcaacg gcatcaggat caagaccgac taccactcac
     7501 tccttgacaa gaaggagaag gagaggttcg aggcccggca aaattggaca agcatcgaga
     7561 acatcaagga gctgaaggcc ggctacatct cccaggtggt gcacaagatc tgtgagctgg
     7621 tggagaagta cgacgccgtc atcgccctgg aggacctcaa cagcggcttc aagaactcaa
     7681 gggtcaaggt ggagaagcag gtgtaccaga agttcgagaa gatgctcatc gacaagctga
     7741 actacatggt ggacaagaag agcaacccct gcgccaccgg cggcgccctc aagggctacc
     7801 agatcaccaa caagtttgag agcttcaaga gcatgtcaac ccagaacggc ttcatcttct
     7861 acatcccggc ctggctcacc tccaagatcg acccctccac cggcttcgtc aacctcctca
     7921 agaccaagta cacctccata gccgacagca agaagttcat ctcctccttc gaccgcatca
     7981 tgtacgtccc tgaggaggac ctcttcgagt tcgccctcga ctacaagaac ttctcaagga
     8041 ccgacgccga ctacatcaag aagtggaagc tctacagcta cggcaaccgc atcaggatct
     8101 tcaggaaccc caagaagaac aacgtcttcg actgggagga ggtgtgcctg acctccgcct
     8161 acaaggagct cttcaacaag tacggcatca actaccagca aggtgacatc agggcgctcc
     8221 tatgcgagca gagcgacaag gccttctact cctccttcat ggccctcatg agcttgatgc
     8281 tccagatgag gaacagcatc accggccgca ccgacgtcga cttcctcatc tcccccgtca
     8341 agaactctga tggcatcttc tacgactcaa ggaactacga ggcccaggag aacgccatcc
     8401 tccccaagaa cgccgacgcc aacggcgcct acaacatcgc caggaaggtg ctctgggcca
     8461 tcggccagtt caagaaggcc gaggatgaga agctggacaa ggtgaagatc gccatctcaa
     8521 acaaggagtg gctggagtac gcccagacct ccgtcaagca ctcaggtcca aagaagaagc
     8581 ggaaggtgtc tggaggttct cctaagaaaa agagaaaagt gtccggcggc tccccgaaga
     8641 agaagcgcaa ggtgtaactg cgaattcggt acgctgaaat caccagtctc tctctacaaa
     8701 tctatctctc tctattttct ccataaataa tgtgtgagta gtttcccgat aagggaaatt
     8761 agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt
     8821 gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact
     8881 aaaatccaga tctcctaaag tccctataga tctttgtcgt gaatataaac cagacacgag
     8941 acgactaaac ctggagccca gacgccgttc gaagctagaa gtaccgctta ggcaggaggc
     9001 cgttagggaa aagatgctaa ggcagggttg gttacgttga ctcccccgta ggtttggttt
     9061 aaatatgatg aagtggacgg aaggaaggag gaagacaagg aaggataagg ttgcaggccc
     9121 tgtgcaaggt aagaagatgg aaatttgata gaggtacgct actatactta tactatacgc
     9181 taagggaatg cttgtattta taccctatac cccctaataa ccccttatca atttaagaaa
     9241 taatccgcat aagcccccgc ttaaaaattg gtatcagagc catgaatagg tctatgacca
     9301 aaactcaaga ggataaaacc tcaccaaaat acgaaagagt tcttaactct aaagataaaa
     9361 gatctttcaa gatcaaaact agttccctca caccgactat aagctaggta gtaactagtg
     9421 cgactgtcta gaatcgcgta tgcgagtgta tctagaatcg cgtatgcgag tgtatgagct
     9481 cgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     9541 cgtgtctcaa aatctctgat gttacattgc acaagataaa ataatatcat catgaacaat
     9601 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     9661 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     9721 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     9781 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     9841 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     9901 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     9961 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
    10021 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
    10081 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
    10141 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
    10201 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
    10261 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
    10321 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
    10381 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
    10441 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
    10501 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
    10561 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10621 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10681 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10741 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10801 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10861 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10921 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10981 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    11041 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    11101 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    11161 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    11221 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
    11281 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
    11341 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
    11401 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
    11461 gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg
    11521 ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct
    11581 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
    11641 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gacttccgac
    11701 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg
    11761 gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggcccacct
//
