LOCUS       pZmUBI-A3A-intron-TaLbCas12a(D832A)-nucNLS-UGI-NLS-P2A-GFP-N7-NLS-G7T       10624 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...ao-en4mbct
VERSION     urn.local...ao-en4mbct
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1986
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /Alternative_Match="99.95%; Adjusted-Interval-0:0/1;
                     ZmUBIL.2 (<a
                     href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBIL"
     intron          978..1987
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     CDS             join(1994..2280,2470..2776)
                     /codon_start=1
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A3Aintron"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; APOBEC3A (<a
                     href=""urn:local:thjac:t-ecoeihu"">A3Aintron</a>)"
                     /label="APOBEC3A"
     intron          2281..2469
                     /vntifkey=15
                     /modified_by="thjac"
                     /label="IV2 intron"
     Linker          2777..2824
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="32-aa linker"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; XTEN (<a
                     href=""urn:local:.:a3-dgycryz"">32-aa linker</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a5-dgycryz"">XTEN</a>"
                     /label="XTEN"
     CDS             2831..6514
                     /%_Identity="99.9457111834962"
                     /Motif="ATGAGCAAGCTGGAGAAGTTCACCAACTGCTACTCACTCTCCAAGACCCT
                     CCGCTTCAAGGCCATCCCCGTCGGCAAGACCCAGGAGAACATCGACAACAAGAGGCTG
                     CTGGTGGAGGATGAGAAGAGGGCCGAGGACTACAAGGGCGTCAAGAAGCTCCTCGACC
                     GGTACTACCTCTCATTCATCAATGATGTCCTCCACTCCATCAAGCTGAAGAACCTCAA
                     CAACTACATCTCCCTCTTCCGCAAGAAGACAAGGACCGAGAAGGAGAACAAGGAGCTG
                     GAGAACCTGGAGATCAACCTCCGCAAGGAGATCGCCAAGGCCTTCAAGGGCAACGAGG
                     GCTACAAGAGCCTCTTCAAGAAGGACATCATCGAGACGATCCTACCGGAGTTCCTGGA
                     TGACAAGGACGAGATCGCCCTGGTGAACAGCTTCAACGGCTTCACCACCGCCTTCACC
                     GGCTTCTTCCGCAACCGGGAGAACATGTTCTCCGAGGAGGCCAAGAGCACCTCCATCG
                     CCTTCAGGTGCATCAACGAGAACCTCACCCGCTACATCTCAAACATGGACATCTTCGA
                     GAAGGTGGACGCCATCTTCGACAAGCATGAGGTCCAGGAGATCAAGGAGAAGATCCTG
                     AACTCCGACTACGACGTGGAGGACTTCTTCGAGGGCGAGTTCTTCAACTTCGTCCTGA
                     CCCAAGAAGGCATCGACGTCTACAACGCCATCATCGGCGGCTTCGTCACCGAGTCAGG
                     TGAGAAGATCAAGGGCCTCAACGAGTACATCAACCTCTACAACCAGAAGACCAAGCAG
                     AAGCTCCCCAAGTTCAAGCCCCTCTACAAGCAGGTGCTCTCCGACCGTGAGAGCTTGA
                     GCTTCTACGGCGAGGGCTACACCTCCGACGAGGAGGTGCTGGAGGTCTTCCGCAACAC
                     CCTCAACAAGAACTCAGAGATCTTCTCCTCCATCAAGAAGCTGGAGAAGCTCTTCAAG
                     AACTTCGACGAGTACTCCTCCGCCGGCATCTTCGTGAAGAACGGCCCGGCCATCTCAA
                     CCATCTCCAAGGACATCTTCGGCGAGTGGAACGTCATCCGTGACAAGTGGAACGCCGA
                     GTACGACGACATCCACCTCAAGAAGAAGGCCGTGGTGACCGAGAAGTACGAGGACGAC
                     CGCCGCAAGAGCTTCAAGAAGATCGGCAGCTTCTCACTGGAGCAGCTCCAGGAGTACG
                     CCGACGCCGACCTCTCCGTGGTGGAGAAGCTGAAGGAGATCATCATCCAGAAGGTGGA
                     CGAGATCTACAAGGTGTACGGCAGCTCTGAGAAGCTCTTCGACGCCGACTTCGTCCTG
                     GAGAAGAGCCTCAAGAAGAACGACGCCGTGGTGGCCATCATGAAGGACCTCCTCGACT
                     CAGTCAAGAGCTTCGAGAACTACATCAAGGCCTTCTTCGGCGAGGGCAAGGAGACAAA
                     CCGCGACGAGAGCTTCTACGGCGACTTCGTCCTCGCCTATGACATCCTCCTCAAGGTG
                     GACCACATCTACGACGCCATCAGGAACTACGTGACCCAGAAGCCCTACTCAAAGGACA
                     AGTTCAAGCTCTACTTCCAGAACCCCCAGTTCATGGGCGGCTGGGACAAGGACAAGGA
                     GACTGACTACCGCGCCACCATCCTCCGCTACGGCTCAAAGTACTACCTCGCCATCATG
                     GACAAGAAGTACGCCAAGTGCCTCCAGAAGATCGACAAGGATGATGTGAACGGCAACT
                     ACGAGAAGATCAACTACAAGCTCCTCCCCGGCCCCAACAAGATGCTACCCAAGGTCTT
                     CTTCTCCAAGAAGTGGATGGCCTACTACAACCCCTCCGAGGACATCCAGAAGATCTAC
                     AAGAACGGCACCTTCAAGAAGGGTGACATGTTCAACCTCAACGACTGCCACAAGCTGA
                     TCGACTTCTTCAAGGACAGCATCTCAAGGTACCCCAAGTGGAGCAACGCCTACGACTT
                     CAACTTCTCTGAGACGGAGAAGTACAAGGACATCGCCGGCTTCTACCGGGAGGTGGAG
                     GAGCAAGGCTACAAGGTGAGCTTCGAGTCCGCCTCCAAGAAGGAGGTGGACAAGCTGG
                     TGGAGGAGGGCAAGCTCTACATGTTCCAGATCTACAACAAGGACTTCTCTGACAAGAG
                     CCACGGCACCCCCAACCTACACACCATGTACTTCAAGCTCCTCTTCGACGAGAACAAC
                     CACGGCCAGATCCGCCTCTCCGGCGGCGCCGAGCTCTTCATGAGGAGGGCCTCCCTCA
                     AGAAGGAGGAGCTGGTGGTTCACCCGGCCAACAGCCCCATCGCCAACAAGAACCCTGA
                     CAACCCCAAGAAGACCACCACCCTCTCCTACGACGTCTACAAGGACAAGAGGTTCTCA
                     GAAGACCAGTACGAGCTCCACATCCCCATCGCCATCAACAAGTGCCCCAAGAACATCT
                     TCAAGATCAACACCGAGGTGAGGGTGCTACTCAAGCATGATGATAACCCCTACGTCAT
                     CGGCATCGCCCGCGGCGAGAGGAACCTCCTCTACATCGTGGTGGTGGACGGCAAGGGC
                     AACATCGTGGAGCAGTACTCCTTGAACGAGATCATCAACAACTTCAACGGCATCAGGA
                     TCAAGACCGACTACCACTCACTCCTTGACAAGAAGGAGAAGGAGAGGTTCGAGGCCCG
                     GCAAAATTGGACAAGCATCGAGAACATCAAGGAGCTGAAGGCCGGCTACATCTCCCAG
                     GTGGTGCACAAGATCTGTGAGCTGGTGGAGAAGTACGACGCCGTCATCGCCCTGGAGG
                     ACCTCAACAGCGGCTTCAAGAACTCAAGGGTCAAGGTGGAGAAGCAGGTGTACCAGAA
                     GTTCGAGAAGATGCTCATCGACAAGCTGAACTACATGGTGGACAAGAAGAGCAACCCC
                     TGCGCCACCGGCGGCGCCCTCAAGGGCTACCAGATCACCAACAAGTTTGAGAGCTTCA
                     AGAGCATGTCAACCCAGAACGGCTTCATCTTCTACATCCCGGCCTGGCTCACCTCCAA
                     GATCGACCCCTCCACCGGCTTCGTCAACCTCCTCAAGACCAAGTACACCTCCATAGCC
                     GACAGCAAGAAGTTCATCTCCTCCTTCGACCGCATCATGTACGTCCCTGAGGAGGACC
                     TCTTCGAGTTCGCCCTCGACTACAAGAACTTCTCAAGGACCGACGCCGACTACATCAA
                     GAAGTGGAAGCTCTACAGCTACGGCAACCGCATCAGGATCTTCAGGAACCCCAAGAAG
                     AACAACGTCTTCGACTGGGAGGAGGTGTGCCTGACCTCCGCCTACAAGGAGCTCTTCA
                     ACAAGTACGGCATCAACTACCAGCAAGGTGACATCAGGGCGCTCCTATGCGAGCAGAG
                     CGACAAGGCCTTCTACTCCTCCTTCATGGCCCTCATGAGCTTGATGCTCCAGATGAGG
                     AACAGCATCACCGGCCGCACCGACGTCGACTTCCTCATCTCCCCCGTCAAGAACTCTG
                     ATGGCATCTTCTACGACTCAAGGAACTACGAGGCCCAGGAGAACGCCATCCTCCCCAA
                     GAACGCCGACGCCAACGGCGCCTACAACATCGCCAGGAAGGTGCTCTGGGCCATCGGC
                     CAGTTCAAGAAGGCCGAGGATGAGAAGCTGGACAAGGTGAAGATCGCCATCTCAAACA
                     AGGAGTGGCTGGAGTACGCCCAGACCTCCGTCAAGCAC"
                     /X-Transferred_From="TaLbCas12a"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:oc-ecpnky2"">TaLbCas12a</a>"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:qo-ecpph81"">TaLbCas12a(D832A)</a>"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TaLbCas12a(D832A)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:19-ecu990z"">TaLbCas12a(D832A)</a>"
                     /label="TaLbCas12a(D832A)"
     CDS             6521..6871
                     /Name="Synthetic coding sequence of the cytidine deaminase
                     base editor, 3rd generation gene   (Zong Y. et al., 2017)"
                     /Clone_Manager_'Draw_As'="Gene"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="nucNLS-UGI-SV40"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:6l-ecue642"">nucNLS-UGI-SV40</a>"
                     /label="nucNLS-UGI-SV40"
     CDS             6872..6928
                     /created_by="User"
                     /Transferred_From="P2A"
                     /Transferred_Similarity="100.00%"
                     /label="P2A"
     CDS             6929..7645
                     /note="A206K"
                     /codon_start=1
                     /transl_table=11
                     /product="GFP"
                     /protein_id="AHE38511.1"
                     /modified_by="thjac"
                     /modified_by="wacae"
                     /Transferred_From="GFP"
                     /Transferred_Similarity="100.00%"
                     /label="GFP"
     Linker          7649..7696
                     /created_by="wacae"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             7697..7945
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      7953..8224
                     /label="G7T"
     Linker          8229..8277
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(8314..8334)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        8395..8445
                     /created_by="chgai"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    8446..9255
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      9402..9990
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          10589..10609
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta
       61 taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt
      121 atacatatat ttaaacttta ctctacgaat aatataatct atagtactac aataatatca
      181 gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt
      241 ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg
      301 caaatagctt cacctatata atacttcatc cattttatta gtacatccat ttagggttta
      361 gggttaatgg tttttataga ctaatttttt tagtacatct attttattct attttagcct
      421 ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa
      481 tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta
      541 aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt
      601 ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca
      661 cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg
      721 ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag
      781 gcggcctcct cctcctctca cggcacggca gctacggggg attcctttcc caccgctcct
      841 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
      901 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
      961 ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc taccttctct
     1021 agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc atgtttgtgt
     1081 tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac
     1141 gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc
     1201 tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt
     1261 tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt
     1321 tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc
     1381 ggagtagaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg
     1441 tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata
     1501 ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg
     1561 gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac
     1621 tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct
     1681 tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat
     1741 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     1801 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     1861 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     1921 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     1981 tctgcaaaca atggaagcct ctcctgcctc tggacccaga cacctgatgg accctcacat
     2041 cttcaccagc aacttcaaca acggcatcgg ccggcacaag acctacctgt gctacgaggt
     2101 ggaacggctg gacaatggca ccagcgtgaa gatggaccag caccggggct ttctgcacaa
     2161 ccaggccaag aatctgctgt gcggcttcta cggcagacac gccgagctga gatttctgga
     2221 cctggtgcct agcctgcagc tggaccctgc tcagatctac agagtgacct ggttcatcag
     2281 gtaagtttct gcttctacct ttgatatata tataataatt atcattaatt agtagtaata
     2341 taatatttca aatatttttt tcaaaataaa agaatgtagt atatagcaat tgcttttctg
     2401 tagtttataa gtgtgtatat tttaatttat aacttttcta atatatgacc aaaatttgtt
     2461 gatgtgcagc tggtccccat gcttctcttg gggctgtgct ggcgaagtgc gcgccttcct
     2521 gcaagagaat actcacgtgc ggctgcggat cttcgccgcc agaatctacg actacgaccc
     2581 tctgtacaaa gaggccctgc agatgctgag agatgccggt gctcaggtgt ccatcatgac
     2641 ctacgacgag ttcaagcact gctgggacac cttcgtggat caccagggct gccctttcca
     2701 gccttgggat ggactggatg agcactctca ggccctgagc ggtagactga gagccatcct
     2761 gcagaaccag ggcaactctg gttctgaaac tcctggaact tcagaatctg ctactcctga
     2821 atccggctcc atgagcaagc tggagaagtt caccaactgc tactcactct ccaagaccct
     2881 ccgcttcaag gccatccccg tcggcaagac ccaggagaac atcgacaaca agaggctgct
     2941 ggtggaggat gagaagaggg ccgaggacta caagggcgtc aagaagctcc tcgaccggta
     3001 ctacctctca ttcatcaatg atgtcctcca ctccatcaag ctgaagaacc tcaacaacta
     3061 catctccctc ttccgcaaga agacaaggac cgagaaggag aacaaggagc tggagaacct
     3121 ggagatcaac ctccgcaagg agatcgccaa ggccttcaag ggcaacgagg gctacaagag
     3181 cctcttcaag aaggacatca tcgagacgat cctaccggag ttcctggatg acaaggacga
     3241 gatcgccctg gtgaacagct tcaacggctt caccaccgcc ttcaccggct tcttcgacaa
     3301 ccgggagaac atgttctccg aggaggccaa gagcacctcc atcgccttca ggtgcatcaa
     3361 cgagaacctc acccgctaca tctcaaacat ggacatcttc gagaaggtgg acgccatctt
     3421 cgacaagcat gaggtccagg agatcaagga gaagatcctg aactccgact acgacgtgga
     3481 ggacttcttc gagggcgagt tcttcaactt cgtcctgacc caagaaggca tcgacgtcta
     3541 caacgccatc atcggcggct tcgtcaccga gtcaggtgag aagatcaagg gcctcaacga
     3601 gtacatcaac ctctacaacc agaagaccaa gcagaagctc cccaagttca agcccctcta
     3661 caagcaggtg ctctccgacc gtgagagctt gagcttctac ggcgagggct acacctccga
     3721 cgaggaggtg ctggaggtct tccgcaacac cctcaacaag aactcagaga tcttctcctc
     3781 catcaagaag ctggagaagc tcttcaagaa cttcgacgag tactcctccg ccggcatctt
     3841 cgtgaagaac ggcccggcca tctcaaccat ctccaaggac atcttcggcg agtggaacgt
     3901 catccgtgac aagtggaacg ccgagtacga cgacatccac ctcaagaaga aggccgtggt
     3961 gaccgagaag tacgaggacg accgccgcaa gagcttcaag aagatcggca gcttctcact
     4021 ggagcagctc caggagtacg ccgacgccga cctctccgtg gtggagaagc tgaaggagat
     4081 catcatccag aaggtggacg agatctacaa ggtgtacggc agctctgaga agctcttcga
     4141 cgccgacttc gtcctggaga agagcctcaa gaagaacgac gccgtggtgg ccatcatgaa
     4201 ggacctcctc gactcagtca agagcttcga gaactacatc aaggccttct tcggcgaggg
     4261 caaggagaca aaccgcgacg agagcttcta cggcgacttc gtcctcgcct atgacatcct
     4321 cctcaaggtg gaccacatct acgacgccat caggaactac gtgacccaga agccctactc
     4381 aaaggacaag ttcaagctct acttccagaa cccccagttc atgggcggct gggacaagga
     4441 caaggagact gactaccgcg ccaccatcct ccgctacggc tcaaagtact acctcgccat
     4501 catggacaag aagtacgcca agtgcctcca gaagatcgac aaggatgatg tgaacggcaa
     4561 ctacgagaag atcaactaca agctcctccc cggccccaac aagatgctac ccaaggtctt
     4621 cttctccaag aagtggatgg cctactacaa cccctccgag gacatccaga agatctacaa
     4681 gaacggcacc ttcaagaagg gtgacatgtt caacctcaac gactgccaca agctgatcga
     4741 cttcttcaag gacagcatct caaggtaccc caagtggagc aacgcctacg acttcaactt
     4801 ctctgagacg gagaagtaca aggacatcgc cggcttctac cgggaggtgg aggagcaagg
     4861 ctacaaggtg agcttcgagt ccgcctccaa gaaggaggtg gacaagctgg tggaggaggg
     4921 caagctctac atgttccaga tctacaacaa ggacttctct gacaagagcc acggcacccc
     4981 caacctacac accatgtact tcaagctcct cttcgacgag aacaaccacg gccagatccg
     5041 cctctccggc ggcgccgagc tcttcatgag gagggcctcc ctcaagaagg aggagctggt
     5101 ggttcacccg gccaacagcc ccatcgccaa caagaaccct gacaacccca agaagaccac
     5161 caccctctcc tacgacgtct acaaggacaa gaggttctca gaagaccagt acgagctcca
     5221 catccccatc gccatcaaca agtgccccaa gaacatcttc aagatcaaca ccgaggtgag
     5281 ggtgctactc aagcatgatg ataaccccta cgtcatcggc atcgcccgcg gcgagaggaa
     5341 cctcctctac atcgtggtgg tggacggcaa gggcaacatc gtggagcagt actccttgaa
     5401 cgagatcatc aacaacttca acggcatcag gatcaagacc gactaccact cactccttga
     5461 caagaaggag aaggagaggt tcgaggcccg gcaaaattgg acaagcatcg agaacatcaa
     5521 ggagctgaag gccggctaca tctcccaggt ggtgcacaag atctgtgagc tggtggagaa
     5581 gtacgacgcc gtcatcgccc tggaggacct caacagcggc ttcaagaact caagggtcaa
     5641 ggtggagaag caggtgtacc agaagttcga gaagatgctc atcgacaagc tgaactacat
     5701 ggtggacaag aagagcaacc cctgcgccac cggcggcgcc ctcaagggct accagatcac
     5761 caacaagttt gagagcttca agagcatgtc aacccagaac ggcttcatct tctacatccc
     5821 ggcctggctc acctccaaga tcgacccctc caccggcttc gtcaacctcc tcaagaccaa
     5881 gtacacctcc atagccgaca gcaagaagtt catctcctcc ttcgaccgca tcatgtacgt
     5941 ccctgaggag gacctcttcg agttcgccct cgactacaag aacttctcaa ggaccgacgc
     6001 cgactacatc aagaagtgga agctctacag ctacggcaac cgcatcagga tcttcaggaa
     6061 ccccaagaag aacaacgtct tcgactggga ggaggtgtgc ctgacctccg cctacaagga
     6121 gctcttcaac aagtacggca tcaactacca gcaaggtgac atcagggcgc tcctatgcga
     6181 gcagagcgac aaggccttct actcctcctt catggccctc atgagcttga tgctccagat
     6241 gaggaacagc atcaccggcc gcaccgacgt cgacttcctc atctcccccg tcaagaactc
     6301 tgatggcatc ttctacgact caaggaacta cgaggcccag gagaacgcca tcctccccaa
     6361 gaacgccgac gccaacggcg cctacaacat cgccaggaag gtgctctggg ccatcggcca
     6421 gttcaagaag gccgaggatg agaagctgga caaggtgaag atcgccatct caaacaagga
     6481 gtggctggag tacgcccaga cctccgtcaa gcactcaggt aagaggccgg cggccaccaa
     6541 gaaggccggc caagccaaga agaagaagac aagagattca ggaggatcca ccaacctctc
     6601 cgacatcatc gagaaggaga ctggcaagca gctggtgatc caggagagca tcttgatgct
     6661 gcctgaggag gtggaggagg tcatcggcaa caagccggag tccgacatcc tcgtccacac
     6721 cgcctacgac gagtccaccg acgagaacgt catgctcctc acctcagatg ctccagagta
     6781 caagccctgg gccttggtga tccaagactc caacggcgag aacaagatca agatgctctc
     6841 cggcggcagc cccaagaaga agaggaaggt tgctactaat ttttctcttc ttaagcaagc
     6901 tggagatgtt gaagaaaatc ctggacccat ggtgagcaag ggcgaggagc tgttcaccgg
     6961 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     7021 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     7081 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcacctacg gcgtgcagtg
     7141 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     7201 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     7261 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     7321 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     7381 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     7441 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     7501 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga
     7561 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     7621 tcacggcatg gacgagctgt acaagtcagg ttctggttca ggttcaggtt ctggatctgg
     7681 aagtggatct ggatcagcgg ccgcatctga attcaagcgt gaagagcaag caaggaaagc
     7741 taaggtgaac aatgagaaaa agacggaaat agtgaaacca gagagttgta gcaatgaagg
     7801 agatgtcaag gatctgaaaa gaaaggactc tgaggatgga aacgagggtg aggaagaaga
     7861 agcttcttcg aaaccgaaaa agccaaaagt tgctctttct catcttcagg acattgacga
     7921 cacagaagct gatcaagaag aagagtaact gcccccgatg agctaagcta gctatatcat
     7981 caatttatgt attacacata atatcgcact cagtctttca tctacggcaa tgtaccagct
     8041 gatataatca gttattgaaa tatttctgaa tttaaacttg catcaataaa tttatgtttt
     8101 tgcttggact ataatacctg acttgttatt ttatcaataa atatttaaac tatatttctt
     8161 tcaagatggg aattaacatc tacaaattgc cttttcttat cgaccatgta ctcgacggcc
     8221 gagtactata agctaggtag taactagtgc gactgtctag aatcgcgtat gcgagtgtat
     8281 gagctcgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     8341 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaataat atcatcatga
     8401 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     8461 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     8521 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     8581 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     8641 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     8701 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     8761 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     8821 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     8881 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     8941 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     9001 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     9061 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     9121 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     9181 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     9241 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     9301 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     9361 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9421 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9481 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9541 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9601 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9661 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     9721 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     9781 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     9841 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     9901 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     9961 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10021 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10081 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    10141 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    10201 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
    10261 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
    10321 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
    10381 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
    10441 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagactt
    10501 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
    10561 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
    10621 acct
//
