LOCUS       pZmUBI_GFP(Q70stop)-N7-NLS-AarI-G7T        5740 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...a3-en4mbco
VERSION     urn.local...a3-en4mbco
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..1986
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /label="ZmUBIL"
     intron          978..1987
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     CDS             2021..2737
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="GFP(Q70stop)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:7i-ecuewpy"">GFP(Q70stop)</a>"
                     /label="GFP(Q70stop)"
     crRNA           complement(2216..2238)
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ABE-Cas12a_2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:32q-eczixvr"">ABE-Cas12a_2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:i2-ecpi3uh"">GFP(Q70stop-R74K)</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:7i-ecuewpy"">GFP(Q70stop)</a>)"
                     /label
     Linker          2741..2788
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker
                     (<a href=""urn:local:.:6j-dgycryx"">(GS)8</a>)"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             2789..3037
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      3045..3316
                     /label="G7T"
     primer          complement(3430..3450)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        3511..3561
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    3562..4371
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      4518..5106
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          5705..5725
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 gtgcagcgtg acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta
       61 taaaaaatta ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt
      121 atacatatat ttaaacttta ctctacgaat aatataatct atagtactac aataatatca
      181 gtgttttaga gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt
      241 ttgacaacag gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg
      301 caaatagctt cacctatata atacttcatc cattttatta gtacatccat ttagggttta
      361 gggttaatgg tttttataga ctaatttttt tagtacatct attttattct attttagcct
      421 ctaaattaag aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa
      481 tagaataaaa taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta
      541 aggaaacatt tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt
      601 ctaacggaca ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca
      661 cggcatctct gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg
      721 ctccgctgtc ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag
      781 gcggcctcct cctcctctca cggcacggca gctacggggg attcctttcc caccgctcct
      841 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
      901 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
      961 ggcacctccg cttcaaggta cgccgctcgt cctccccccc cccccctctc taccttctct
     1021 agatcggcgt tccggtccat ggttagggcc cggtagttct acttctgttc atgtttgtgt
     1081 tagatccgtg tttgtgttag atccgtgctg ctagcgttcg tacacggatg cgacctgtac
     1141 gtcagacacg ttctgattgc taacttgcca gtgtttctct ttggggaatc ctgggatggc
     1201 tctagccgtt ccgcagacgg gatcgatttc atgatttttt ttgtttcgtt gcatagggtt
     1261 tggtttgccc ttttccttta tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt
     1321 tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg ttgggcggtc gttctagatc
     1381 ggagtagaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg
     1441 tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata
     1501 ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg
     1561 gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac
     1621 tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct
     1681 tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat
     1741 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
     1801 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
     1861 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
     1921 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
     1981 tctgcaaaca gtattcagtc gactggtacc aacaggctcc atggtgagca agggcgagga
     2041 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     2101 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     2161 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     2221 cggcgtgtag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2281 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2341 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2401 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     2461 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     2521 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2581 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2641 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2701 cgccgggatc actctcggca tggacgagct gtacaagtca ggttctggtt caggttcagg
     2761 ttctggatct ggaagtggat ctggatcagc ggccgcatct gaattcaagc gtgaagagca
     2821 agcaaggaaa gctaaggtga acaatgagaa aaagacggaa atagtgaaac cagagagttg
     2881 tagcaatgaa ggagatgtca aggatctgaa aagaaaggac tctgaggatg gaaacgaggg
     2941 tgaggaagaa gaagcttctt cgaaaccgaa aaagccaaaa gttgctcttt ctcatcttca
     3001 ggacattgac gacacagaag ctgatcaaga agaagagtaa ctgcccccga tgagctaagc
     3061 tagctatatc atcaatttat gtattacaca taatatcgca ctcagtcttt catctacggc
     3121 aatgtaccag ctgatataat cagttattga aatatttctg aatttaaact tgcatcaata
     3181 aatttatgtt tttgcttgga ctataatacc tgacttgtta ttttatcaat aaatatttaa
     3241 actatatttc tttcaagatg ggaattaaca tctacaaatt gccttttctt atcgaccatg
     3301 tactcgacgg ccgagtacta taagctaggt agtaactagt acctggatgc aggtggacac
     3361 ctgcagctgt atctagaatc gcgtatgcga gtgtatgagc tcgatatccc ctatagtgag
     3421 tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga
     3481 tgttacattg cacaagataa aataatatca tcatgaacaa taaaactgtc tgcttacata
     3541 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     3601 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     3661 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     3721 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     3781 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     3841 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     3901 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     3961 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     4021 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     4081 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     4141 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     4201 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     4261 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     4321 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     4381 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     4441 tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa
     4501 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4561 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4621 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4681 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4741 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4801 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4861 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4921 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4981 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5041 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5101 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5161 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5221 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5281 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5341 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg
     5401 cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct
     5461 tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc
     5521 gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca
     5581 ccgacaaaca acagataaaa cgaaaggccc agacttccga ctgagccttt cgttttattt
     5641 gatgcctggc agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga
     5701 cgttgtaaaa cgacggccag tcttaagctc gggcccacct
//
