LOCUS       pGG-A-TaU3-gRNA-GFP-ABErep2-B        3314 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...a2-en4mbcn
VERSION     urn.local...a2-en4mbcn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2k-c4nnd55"">A</a>"
                     /label="A"
     promoter        12..536
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TaU3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pTaU3
                     (<a href=""urn:local:.:6x-dgycryx"">TaU3</a>)"
                     /label="pTaU3"
     gRNA            537..556
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ABE_Cas9"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:32m-eczixvc"">ABE_Cas9</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:7i-ecuewpy"">GFP(Q70stop)</a>)"
                     /label
     Scaffold        557..639
                     /%_Identity=100
                     /Motif="GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAAC
                     TTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTT"
                     /annotation_group="Scaffold: 451 -> 533"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SpCas9 Scaffold"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Scaffold
                     (<a href=""urn:local:.:7o-dgycryx"">SpCas9 Scaffold</a>)"
                     /label="Scaffold"
     GG              complement(640..650)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:7z-dgycryx"">B</a>"
                     /label="B"
     primer          complement(670..690)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1170..1274
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1275..2135
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2306..2894
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3258..3279
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaacc tcatgaatcc aaaccacacg gagttcaaat tcccacagat taaggctcgt
       61 ccgtcgcaca aggtaatgtg tgaatattat atctgtcgtg caaaattgcc tggcctgcac
      121 aattgctgtt atagttggcg gcagggagag ttttaacatt gactagcgtg ctgataattt
      181 gtgagaaata ataattgaca agtagatact gacatttgag aagagcttct gaactgttat
      241 tagtaacaaa aatggaaagc tgatgcacgg aaaaaggaaa gaaaaagcca tacttttttt
      301 taggtaggaa aagaaaaagc catacgagac tgatgtctct cagatgggcc gggatctgtc
      361 tatctagcag gcagcagccc accaacctca cgggccagca attacgagtc cttctaaaag
      421 ctcccgccga ggggcgctgg cgctgctgtg cagcagcacg tctaacatta gtcccacctc
      481 gccagtttac agggagcaga accagcttat aagcggaggc gcggcaccaa gaagcagcac
      541 tacacgccgt aggtcagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
      601 atcaacttga aaaagtggca ccgagtcggt gcttttttta acatgagacc gaattctcgc
      661 cctatagtga gtcgtattac ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      721 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      781 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
      841 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
      901 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
      961 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     1021 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     1081 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     1141 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1201 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1261 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1321 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1381 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1441 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1501 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1561 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1621 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1681 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1741 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1801 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1861 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1921 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1981 gtgagcgtgg ctctcgaggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2041 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2101 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2161 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2221 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2281 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2341 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2401 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2461 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2521 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2581 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2641 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2701 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2761 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2821 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2881 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2941 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3001 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3061 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3121 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3181 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     3241 gtatgttgtg tggaattgtg agcggataac aatttcacac gagctattta ggtgacacta
     3301 tagaagtgaa gctt
//
