LOCUS       pZmUBI-A3A-intron-TaLbCas12a(D832A)-nucNLS-UGI-NLS-G7T       11786 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...a1-en4mbcn
VERSION     urn.local...a1-en4mbcn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..864
                     /regulatory_class="promoter"
                     /gene="CaMV 35S"
                     /note="internal BsaI recognition site removed"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:6v-dgycryx"">pCaMV 35S</a>)"
                     /label="p35S"
     CDS             899..1606
                     /created_by="User"
                     /modified_by="User"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="mCherry"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:3a-c4nnd56"">mCherry</a>"
                     /label="mCherry"
     Linker          1610..1657
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker
                     (<a href=""urn:local:thjac:1g-c4nnd54"">(GS)8</a>)"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             1658..1906
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      1914..2181
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /label="NOST"
     promoter        2210..4195
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /label="ZmUBIL"
     intron          3187..4196
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     CDS             join(4203..4489,4679..4985)
                     /codon_start=1
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="A3Aintron"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0,
                     Adjusted-Interval-1:0/0; APOBEC3A (<a
                     href=""urn:local:thjac:t-ecoeihu"">A3Aintron</a>)"
                     /label="APOBEC3A"
     intron          4490..4678
                     /vntifkey=15
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Gus-intron"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; IV2
                     Intron (<a
                     href=""urn:local:.:8x-dgycryy"">Gus-intron</a>)"
                     /label="IV2 Intron"
     Linker          4986..5033
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="32-aa linker"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; XTEN (<a
                     href=""urn:local:.:a3-dgycryz"">32-aa linker</a>)"
                     /label="XTEN"
     CDS             5040..8723
                     /%_Identity="99.9457111834962"
                     /Motif="ATGAGCAAGCTGGAGAAGTTCACCAACTGCTACTCACTCTCCAAGACCCT
                     CCGCTTCAAGGCCATCCCCGTCGGCAAGACCCAGGAGAACATCGACAACAAGAGGCTG
                     CTGGTGGAGGATGAGAAGAGGGCCGAGGACTACAAGGGCGTCAAGAAGCTCCTCGACC
                     GGTACTACCTCTCATTCATCAATGATGTCCTCCACTCCATCAAGCTGAAGAACCTCAA
                     CAACTACATCTCCCTCTTCCGCAAGAAGACAAGGACCGAGAAGGAGAACAAGGAGCTG
                     GAGAACCTGGAGATCAACCTCCGCAAGGAGATCGCCAAGGCCTTCAAGGGCAACGAGG
                     GCTACAAGAGCCTCTTCAAGAAGGACATCATCGAGACGATCCTACCGGAGTTCCTGGA
                     TGACAAGGACGAGATCGCCCTGGTGAACAGCTTCAACGGCTTCACCACCGCCTTCACC
                     GGCTTCTTCCGCAACCGGGAGAACATGTTCTCCGAGGAGGCCAAGAGCACCTCCATCG
                     CCTTCAGGTGCATCAACGAGAACCTCACCCGCTACATCTCAAACATGGACATCTTCGA
                     GAAGGTGGACGCCATCTTCGACAAGCATGAGGTCCAGGAGATCAAGGAGAAGATCCTG
                     AACTCCGACTACGACGTGGAGGACTTCTTCGAGGGCGAGTTCTTCAACTTCGTCCTGA
                     CCCAAGAAGGCATCGACGTCTACAACGCCATCATCGGCGGCTTCGTCACCGAGTCAGG
                     TGAGAAGATCAAGGGCCTCAACGAGTACATCAACCTCTACAACCAGAAGACCAAGCAG
                     AAGCTCCCCAAGTTCAAGCCCCTCTACAAGCAGGTGCTCTCCGACCGTGAGAGCTTGA
                     GCTTCTACGGCGAGGGCTACACCTCCGACGAGGAGGTGCTGGAGGTCTTCCGCAACAC
                     CCTCAACAAGAACTCAGAGATCTTCTCCTCCATCAAGAAGCTGGAGAAGCTCTTCAAG
                     AACTTCGACGAGTACTCCTCCGCCGGCATCTTCGTGAAGAACGGCCCGGCCATCTCAA
                     CCATCTCCAAGGACATCTTCGGCGAGTGGAACGTCATCCGTGACAAGTGGAACGCCGA
                     GTACGACGACATCCACCTCAAGAAGAAGGCCGTGGTGACCGAGAAGTACGAGGACGAC
                     CGCCGCAAGAGCTTCAAGAAGATCGGCAGCTTCTCACTGGAGCAGCTCCAGGAGTACG
                     CCGACGCCGACCTCTCCGTGGTGGAGAAGCTGAAGGAGATCATCATCCAGAAGGTGGA
                     CGAGATCTACAAGGTGTACGGCAGCTCTGAGAAGCTCTTCGACGCCGACTTCGTCCTG
                     GAGAAGAGCCTCAAGAAGAACGACGCCGTGGTGGCCATCATGAAGGACCTCCTCGACT
                     CAGTCAAGAGCTTCGAGAACTACATCAAGGCCTTCTTCGGCGAGGGCAAGGAGACAAA
                     CCGCGACGAGAGCTTCTACGGCGACTTCGTCCTCGCCTATGACATCCTCCTCAAGGTG
                     GACCACATCTACGACGCCATCAGGAACTACGTGACCCAGAAGCCCTACTCAAAGGACA
                     AGTTCAAGCTCTACTTCCAGAACCCCCAGTTCATGGGCGGCTGGGACAAGGACAAGGA
                     GACTGACTACCGCGCCACCATCCTCCGCTACGGCTCAAAGTACTACCTCGCCATCATG
                     GACAAGAAGTACGCCAAGTGCCTCCAGAAGATCGACAAGGATGATGTGAACGGCAACT
                     ACGAGAAGATCAACTACAAGCTCCTCCCCGGCCCCAACAAGATGCTACCCAAGGTCTT
                     CTTCTCCAAGAAGTGGATGGCCTACTACAACCCCTCCGAGGACATCCAGAAGATCTAC
                     AAGAACGGCACCTTCAAGAAGGGTGACATGTTCAACCTCAACGACTGCCACAAGCTGA
                     TCGACTTCTTCAAGGACAGCATCTCAAGGTACCCCAAGTGGAGCAACGCCTACGACTT
                     CAACTTCTCTGAGACGGAGAAGTACAAGGACATCGCCGGCTTCTACCGGGAGGTGGAG
                     GAGCAAGGCTACAAGGTGAGCTTCGAGTCCGCCTCCAAGAAGGAGGTGGACAAGCTGG
                     TGGAGGAGGGCAAGCTCTACATGTTCCAGATCTACAACAAGGACTTCTCTGACAAGAG
                     CCACGGCACCCCCAACCTACACACCATGTACTTCAAGCTCCTCTTCGACGAGAACAAC
                     CACGGCCAGATCCGCCTCTCCGGCGGCGCCGAGCTCTTCATGAGGAGGGCCTCCCTCA
                     AGAAGGAGGAGCTGGTGGTTCACCCGGCCAACAGCCCCATCGCCAACAAGAACCCTGA
                     CAACCCCAAGAAGACCACCACCCTCTCCTACGACGTCTACAAGGACAAGAGGTTCTCA
                     GAAGACCAGTACGAGCTCCACATCCCCATCGCCATCAACAAGTGCCCCAAGAACATCT
                     TCAAGATCAACACCGAGGTGAGGGTGCTACTCAAGCATGATGATAACCCCTACGTCAT
                     CGGCATCGCCCGCGGCGAGAGGAACCTCCTCTACATCGTGGTGGTGGACGGCAAGGGC
                     AACATCGTGGAGCAGTACTCCTTGAACGAGATCATCAACAACTTCAACGGCATCAGGA
                     TCAAGACCGACTACCACTCACTCCTTGACAAGAAGGAGAAGGAGAGGTTCGAGGCCCG
                     GCAAAATTGGACAAGCATCGAGAACATCAAGGAGCTGAAGGCCGGCTACATCTCCCAG
                     GTGGTGCACAAGATCTGTGAGCTGGTGGAGAAGTACGACGCCGTCATCGCCCTGGAGG
                     ACCTCAACAGCGGCTTCAAGAACTCAAGGGTCAAGGTGGAGAAGCAGGTGTACCAGAA
                     GTTCGAGAAGATGCTCATCGACAAGCTGAACTACATGGTGGACAAGAAGAGCAACCCC
                     TGCGCCACCGGCGGCGCCCTCAAGGGCTACCAGATCACCAACAAGTTTGAGAGCTTCA
                     AGAGCATGTCAACCCAGAACGGCTTCATCTTCTACATCCCGGCCTGGCTCACCTCCAA
                     GATCGACCCCTCCACCGGCTTCGTCAACCTCCTCAAGACCAAGTACACCTCCATAGCC
                     GACAGCAAGAAGTTCATCTCCTCCTTCGACCGCATCATGTACGTCCCTGAGGAGGACC
                     TCTTCGAGTTCGCCCTCGACTACAAGAACTTCTCAAGGACCGACGCCGACTACATCAA
                     GAAGTGGAAGCTCTACAGCTACGGCAACCGCATCAGGATCTTCAGGAACCCCAAGAAG
                     AACAACGTCTTCGACTGGGAGGAGGTGTGCCTGACCTCCGCCTACAAGGAGCTCTTCA
                     ACAAGTACGGCATCAACTACCAGCAAGGTGACATCAGGGCGCTCCTATGCGAGCAGAG
                     CGACAAGGCCTTCTACTCCTCCTTCATGGCCCTCATGAGCTTGATGCTCCAGATGAGG
                     AACAGCATCACCGGCCGCACCGACGTCGACTTCCTCATCTCCCCCGTCAAGAACTCTG
                     ATGGCATCTTCTACGACTCAAGGAACTACGAGGCCCAGGAGAACGCCATCCTCCCCAA
                     GAACGCCGACGCCAACGGCGCCTACAACATCGCCAGGAAGGTGCTCTGGGCCATCGGC
                     CAGTTCAAGAAGGCCGAGGATGAGAAGCTGGACAAGGTGAAGATCGCCATCTCAAACA
                     AGGAGTGGCTGGAGTACGCCCAGACCTCCGTCAAGCAC"
                     /X-Transferred_From="TaLbCas12a"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:oc-ecpnky2"">TaLbCas12a</a>"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:qo-ecpph81"">TaLbCas12a(D832A)</a>"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TaLbCas12a(D832A)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:19-ecu990z"">TaLbCas12a(D832A)</a>"
                     /label="TaLbCas12a(D832A)"
     CDS             8730..9080
                     /Name="Synthetic coding sequence of the cytidine deaminase
                     base editor, 3rd generation gene   (Zong Y. et al., 2017)"
                     /Clone_Manager_'Draw_As'="Gene"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="nucNLS-UGI-SV40"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:6l-ecue642"">nucNLS-UGI-SV40</a>"
                     /modified_by="mamee"
                     /label="nucNLS-UGI-SV40"
     terminator      9091..9362
                     /label="G7T"
     Linker          9367..9415
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(9476..9496)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        9557..9607
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    9608..10417
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      10564..11152
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          11751..11771
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ccgatccatt cggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
       61 tggttagaga ggcttacgca gcacgtctca tcaagacgat ctacccgagc aataatctcc
      121 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
      181 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
      241 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
      301 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
      361 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
      421 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
      481 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
      541 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
      601 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
      661 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      721 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      781 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      841 tggagagaac acgggggacg agctaacagt attcagtcga ctggtaccaa caggctccat
      901 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      961 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1021 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1081 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1141 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1201 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     1261 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1321 gcagaagaag acgatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1381 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1441 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1501 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     1561 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtcag gttctggttc
     1621 aggttcaggt tctggatctg gaagtggatc tggatcagcg gccgcatctg aattcaagcg
     1681 tgaagagcaa gcaaggaaag ctaaggtgaa caatgagaaa aagacggaaa tagtgaaacc
     1741 agagagttgt agcaatgaag gagatgtcaa ggatctgaaa agaaaggact ctgaggatgg
     1801 aaacgagggt gaggaagaag aagcttcttc gaaaccgaaa aagccaaaag ttgctctttc
     1861 tcatcttcag gacattgacg acacagaagc tgatcaagaa gaagagtaac tgcctagagt
     1921 caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     1981 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     2041 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     2101 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     2161 tcatctatgt tactagatcg aactataagc taggtagtaa ctagtacctg tgcagcgtga
     2221 cccggtcgtg cccctctcta gagataatga gcattgcatg tctaagttat aaaaaattac
     2281 cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta tacatatatt
     2341 taaactttac tctacgaata atataatcta tagtactaca ataatatcag tgttttagag
     2401 aatcatataa atgaacagtt agacatggtc taaaggacaa ttgagtattt tgacaacagg
     2461 actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc aaatagcttc
     2521 acctatataa tacttcatcc attttattag tacatccatt tagggtttag ggttaatggt
     2581 ttttatagac taattttttt agtacatcta ttttattcta ttttagcctc taaattaaga
     2641 aaactaaaac tctattttag tttttttatt taataattta gatataaaat agaataaaat
     2701 aaagtgacta aaaattaaac aaataccctt taagaaatta aaaaaactaa ggaaacattt
     2761 ttcttgtttc gagtagataa tgccagcctg ttaaacgccg tcgacgagtc taacggacac
     2821 caaccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg
     2881 tcgctgcctc tggacccctc tcgagagttc cgctccaccg ttggacttgc tccgctgtcg
     2941 gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg cggcctcctc
     3001 ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct
     3061 tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt
     3121 gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc
     3181 ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt
     3241 ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt
     3301 ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt
     3361 tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc
     3421 cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct
     3481 tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt
     3541 tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt
     3601 ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata
     3661 ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt
     3721 tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat
     3781 gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact
     3841 acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg
     3901 agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac
     3961 tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct
     4021 atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat
     4081 ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg
     4141 cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt ctgcaaacaa
     4201 tggaagcctc tcctgcctct ggacccagac acctgatgga ccctcacatc ttcaccagca
     4261 acttcaacaa cggcatcggc cggcacaaga cctacctgtg ctacgaggtg gaacggctgg
     4321 acaatggcac cagcgtgaag atggaccagc accggggctt tctgcacaac caggccaaga
     4381 atctgctgtg cggcttctac ggcagacacg ccgagctgag atttctggac ctggtgccta
     4441 gcctgcagct ggaccctgct cagatctaca gagtgacctg gttcatcagg taagtttctg
     4501 cttctacctt tgatatatat ataataatta tcattaatta gtagtaatat aatatttcaa
     4561 atattttttt caaaataaaa gaatgtagta tatagcaatt gcttttctgt agtttataag
     4621 tgtgtatatt ttaatttata acttttctaa tatatgacca aaatttgttg atgtgcagct
     4681 ggtccccatg cttctcttgg ggctgtgctg gcgaagtgcg cgccttcctg caagagaata
     4741 ctcacgtgcg gctgcggatc ttcgccgcca gaatctacga ctacgaccct ctgtacaaag
     4801 aggccctgca gatgctgaga gatgccggtg ctcaggtgtc catcatgacc tacgacgagt
     4861 tcaagcactg ctgggacacc ttcgtggatc accagggctg ccctttccag ccttgggatg
     4921 gactggatga gcactctcag gccctgagcg gtagactgag agccatcctg cagaaccagg
     4981 gcaactctgg ttctgaaact cctggaactt cagaatctgc tactcctgaa tccggctcca
     5041 tgagcaagct ggagaagttc accaactgct actcactctc caagaccctc cgcttcaagg
     5101 ccatccccgt cggcaagacc caggagaaca tcgacaacaa gaggctgctg gtggaggatg
     5161 agaagagggc cgaggactac aagggcgtca agaagctcct cgaccggtac tacctctcat
     5221 tcatcaatga tgtcctccac tccatcaagc tgaagaacct caacaactac atctccctct
     5281 tccgcaagaa gacaaggacc gagaaggaga acaaggagct ggagaacctg gagatcaacc
     5341 tccgcaagga gatcgccaag gccttcaagg gcaacgaggg ctacaagagc ctcttcaaga
     5401 aggacatcat cgagacgatc ctaccggagt tcctggatga caaggacgag atcgccctgg
     5461 tgaacagctt caacggcttc accaccgcct tcaccggctt cttcgacaac cgggagaaca
     5521 tgttctccga ggaggccaag agcacctcca tcgccttcag gtgcatcaac gagaacctca
     5581 cccgctacat ctcaaacatg gacatcttcg agaaggtgga cgccatcttc gacaagcatg
     5641 aggtccagga gatcaaggag aagatcctga actccgacta cgacgtggag gacttcttcg
     5701 agggcgagtt cttcaacttc gtcctgaccc aagaaggcat cgacgtctac aacgccatca
     5761 tcggcggctt cgtcaccgag tcaggtgaga agatcaaggg cctcaacgag tacatcaacc
     5821 tctacaacca gaagaccaag cagaagctcc ccaagttcaa gcccctctac aagcaggtgc
     5881 tctccgaccg tgagagcttg agcttctacg gcgagggcta cacctccgac gaggaggtgc
     5941 tggaggtctt ccgcaacacc ctcaacaaga actcagagat cttctcctcc atcaagaagc
     6001 tggagaagct cttcaagaac ttcgacgagt actcctccgc cggcatcttc gtgaagaacg
     6061 gcccggccat ctcaaccatc tccaaggaca tcttcggcga gtggaacgtc atccgtgaca
     6121 agtggaacgc cgagtacgac gacatccacc tcaagaagaa ggccgtggtg accgagaagt
     6181 acgaggacga ccgccgcaag agcttcaaga agatcggcag cttctcactg gagcagctcc
     6241 aggagtacgc cgacgccgac ctctccgtgg tggagaagct gaaggagatc atcatccaga
     6301 aggtggacga gatctacaag gtgtacggca gctctgagaa gctcttcgac gccgacttcg
     6361 tcctggagaa gagcctcaag aagaacgacg ccgtggtggc catcatgaag gacctcctcg
     6421 actcagtcaa gagcttcgag aactacatca aggccttctt cggcgagggc aaggagacaa
     6481 accgcgacga gagcttctac ggcgacttcg tcctcgccta tgacatcctc ctcaaggtgg
     6541 accacatcta cgacgccatc aggaactacg tgacccagaa gccctactca aaggacaagt
     6601 tcaagctcta cttccagaac ccccagttca tgggcggctg ggacaaggac aaggagactg
     6661 actaccgcgc caccatcctc cgctacggct caaagtacta cctcgccatc atggacaaga
     6721 agtacgccaa gtgcctccag aagatcgaca aggatgatgt gaacggcaac tacgagaaga
     6781 tcaactacaa gctcctcccc ggccccaaca agatgctacc caaggtcttc ttctccaaga
     6841 agtggatggc ctactacaac ccctccgagg acatccagaa gatctacaag aacggcacct
     6901 tcaagaaggg tgacatgttc aacctcaacg actgccacaa gctgatcgac ttcttcaagg
     6961 acagcatctc aaggtacccc aagtggagca acgcctacga cttcaacttc tctgagacgg
     7021 agaagtacaa ggacatcgcc ggcttctacc gggaggtgga ggagcaaggc tacaaggtga
     7081 gcttcgagtc cgcctccaag aaggaggtgg acaagctggt ggaggagggc aagctctaca
     7141 tgttccagat ctacaacaag gacttctctg acaagagcca cggcaccccc aacctacaca
     7201 ccatgtactt caagctcctc ttcgacgaga acaaccacgg ccagatccgc ctctccggcg
     7261 gcgccgagct cttcatgagg agggcctccc tcaagaagga ggagctggtg gttcacccgg
     7321 ccaacagccc catcgccaac aagaaccctg acaaccccaa gaagaccacc accctctcct
     7381 acgacgtcta caaggacaag aggttctcag aagaccagta cgagctccac atccccatcg
     7441 ccatcaacaa gtgccccaag aacatcttca agatcaacac cgaggtgagg gtgctactca
     7501 agcatgatga taacccctac gtcatcggca tcgcccgcgg cgagaggaac ctcctctaca
     7561 tcgtggtggt ggacggcaag ggcaacatcg tggagcagta ctccttgaac gagatcatca
     7621 acaacttcaa cggcatcagg atcaagaccg actaccactc actccttgac aagaaggaga
     7681 aggagaggtt cgaggcccgg caaaattgga caagcatcga gaacatcaag gagctgaagg
     7741 ccggctacat ctcccaggtg gtgcacaaga tctgtgagct ggtggagaag tacgacgccg
     7801 tcatcgccct ggaggacctc aacagcggct tcaagaactc aagggtcaag gtggagaagc
     7861 aggtgtacca gaagttcgag aagatgctca tcgacaagct gaactacatg gtggacaaga
     7921 agagcaaccc ctgcgccacc ggcggcgccc tcaagggcta ccagatcacc aacaagtttg
     7981 agagcttcaa gagcatgtca acccagaacg gcttcatctt ctacatcccg gcctggctca
     8041 cctccaagat cgacccctcc accggcttcg tcaacctcct caagaccaag tacacctcca
     8101 tagccgacag caagaagttc atctcctcct tcgaccgcat catgtacgtc cctgaggagg
     8161 acctcttcga gttcgccctc gactacaaga acttctcaag gaccgacgcc gactacatca
     8221 agaagtggaa gctctacagc tacggcaacc gcatcaggat cttcaggaac cccaagaaga
     8281 acaacgtctt cgactgggag gaggtgtgcc tgacctccgc ctacaaggag ctcttcaaca
     8341 agtacggcat caactaccag caaggtgaca tcagggcgct cctatgcgag cagagcgaca
     8401 aggccttcta ctcctccttc atggccctca tgagcttgat gctccagatg aggaacagca
     8461 tcaccggccg caccgacgtc gacttcctca tctcccccgt caagaactct gatggcatct
     8521 tctacgactc aaggaactac gaggcccagg agaacgccat cctccccaag aacgccgacg
     8581 ccaacggcgc ctacaacatc gccaggaagg tgctctgggc catcggccag ttcaagaagg
     8641 ccgaggatga gaagctggac aaggtgaaga tcgccatctc aaacaaggag tggctggagt
     8701 acgcccagac ctccgtcaag cactcaggta agaggccggc ggccaccaag aaggccggcc
     8761 aagccaagaa gaagaagaca agagattcag gaggatccac caacctctcc gacatcatcg
     8821 agaaggagac tggcaagcag ctggtgatcc aggagagcat cttgatgctg cctgaggagg
     8881 tggaggaggt catcggcaac aagccggagt ccgacatcct cgtccacacc gcctacgacg
     8941 agtccaccga cgagaacgtc atgctcctca cctcagatgc tccagagtac aagccctggg
     9001 ccttggtgat ccaagactcc aacggcgaga acaagatcaa gatgctctcc ggcggcagcc
     9061 ccaagaagaa gaggaaggtt tagtaactgc ccccgatgag ctaagctagc tatatcatca
     9121 atttatgtat tacacataat atcgcactca gtctttcatc tacggcaatg taccagctga
     9181 tataatcagt tattgaaata tttctgaatt taaacttgca tcaataaatt tatgtttttg
     9241 cttggactat aatacctgac ttgttatttt atcaataaat atttaaacta tatttctttc
     9301 aagatgggaa ttaacatcta caaattgcct tttcttatcg accatgtact cgacggccga
     9361 gtactataag ctaggtagta actagtgcga ctgtctagaa tcgcgtatgc gagtgtatct
     9421 agaatcgcgt atgcgagtgt atgagctcga tatcccctat agtgagtcgt attacatggt
     9481 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
     9541 agataaaata atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag
     9601 gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga
     9661 tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat
     9721 ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag
     9781 cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc
     9841 tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc
     9901 gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat
     9961 tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc
    10021 ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt
    10081 ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa
    10141 agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc
    10201 acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt
    10261 cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc
    10321 tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa
    10381 attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta
    10441 acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct
    10501 taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    10561 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    10621 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    10681 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    10741 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10801 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    10861 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    10921 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10981 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    11041 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    11101 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    11161 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    11221 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    11281 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    11341 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    11401 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca
    11461 ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga
    11521 tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg
    11581 ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
    11641 ataaaacgaa aggcccagac ttccgactga gcctttcgtt ttatttgatg cctggcagtt
    11701 ccctactctc gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac
    11761 ggccagtctt aagctcgggc ccacct
//
