LOCUS       pGG-C-GFP(Q70stop)-D        3405 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...9z-en4mbcn
VERSION     urn.local...9z-en4mbcn
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     GG              1..11
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="C"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:2i-c4nnd55"">C</a>"
                     /label="C"
     CDS             14..730
                     /modified_by="thjac"
                     /label="GFP(Q70stop)"
     crRNA           complement(209..231)
                     /created_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ABE-Cas12a_2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:32q-eczixvr"">ABE-Cas12a_2</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:i2-ecpi3uh"">GFP(Q70stop-R74K)</a>)
                     "
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;  (<a
                     href=""urn:local:thjac:7i-ecuewpy"">GFP(Q70stop)</a>)"
                     /label
     GG              complement(731..741)
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="D"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:81-dgycryx"">D</a>"
                     /label="D"
     primer          complement(761..781)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_rev"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_rev (pGG-B-HA-TurboID-linker-C - GG_SEQ_rev)"
                     /label="GG_SEQ_rev"
     promoter        1261..1365
                     /created_by="mamee"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; amp (<a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>)"
                     /label="amp"
     misc_feature    1366..2226
                     /X-Transferred_From="AmpR"
                     /X-Transferred_Similarity="100.00%"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8i-dgycryy"">AmpR</a>"
                     /label="AmpR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      2397..2985
                     /created_by="thjac"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:thjac:18-c4nnd53"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          3349..3370
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pGG-B-HA-TurboID-linker-C - GG_SEQ_fw"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     GG_SEQ_fw (pGG-B-HA-TurboID-linker-C - GG_SEQ_fw)"
                     /label="GG_SEQ_fw"
ORIGIN      
        1 ggtctcaggc tccatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
       61 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      121 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      181 gccctggccc accctcgtga ccaccctgac ctacggcgtg tagtgcttca gccgctaccc
      241 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      301 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      361 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      421 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
      481 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      541 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
      601 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
      661 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
      721 gctgtacaag tcagtgagac cgaattctcg ccctatagtg agtcgtatta cctggccgtc
      781 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
      841 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
      901 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
      961 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     1021 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     1081 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     1141 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     1201 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     1261 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1321 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     1381 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1441 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1501 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1561 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     1621 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1681 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1741 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1801 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1861 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     1921 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1981 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2041 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gctctcgagg tatcattgca
     2101 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2161 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2221 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2281 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2341 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2401 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2461 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2521 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2581 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2641 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2701 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2761 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2821 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2881 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2941 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3001 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     3061 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     3121 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     3181 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     3241 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     3301 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     3361 caatttcaca cgagctattt aggtgacact atagaagtga agctt
//
