LOCUS       pZmUBI-nucNLS-nCas9-PmCDA1-SV40-NLS-G7T       11987 bp    DNA     circular UNA 21-APR-2023
DEFINITION  .
ACCESSION   urn.local...9v-en4mbcm
VERSION     urn.local...9v-en4mbcm
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        1..864
                     /regulatory_class="promoter"
                     /gene="CaMV 35S"
                     /note="internal BsaI recognition site removed"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCaMV 35S"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; p35S (<a
                     href=""urn:local:.:6v-dgycryx"">pCaMV 35S</a>)"
                     /label="p35S"
     CDS             899..1606
                     /label="mCherry"
     Linker          1610..1657
                     /created_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="(GS)8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Linker
                     (<a href=""urn:local:.:6j-dgycryx"">(GS)8</a>)"
                     /modified_by="mamee"
                     /label="Linker"
     NLS             1658..1906
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS N7"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; N7 (<a
                     href=""urn:local:.:9y-dgycryz"">NLS N7</a>)"
                     /label="N7"
     terminator      1914..2181
                     /Transferred_From="NOST"
                     /Transferred_Similarity="100.00%"
                     /label="NOST"
     promoter        2210..4195
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ZmUBIL
                     (<a href=""urn:local:.:6u-dgycryx"">pZmUBIL</a>)"
                     /label="ZmUBIL"
     intron          3187..4196
                     /created_by="lapau"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pZmUBIL.2"
                     /Transferred_Similarity="99.90%"
                     /Primary_Match="99.90%; Adjusted-Interval-0:0/0; ZmUBI
                     intron (<a href=""urn:local:.:6s-dgycryx"">pZmUBIL.2</a>)"
                     /label="ZmUBI intron"
     NLS             4203..4250
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS Nucleoplasmin"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Nucleoplasmin (<a href=""urn:local:.:9z-dgycryz"">NLS
                     Nucleoplasmin</a>)"
                     /label="Nucleoplasmin"
     CDS             4263..8366
                     /X-Transferred_From="Cas9PTA"
                     /X-Transferred_Similarity="99.98%"
                     /X-Primary_Match="99.98%; Adjusted-Interval-0:0/0; CAS9
                     (<a href=""urn:local:thjac:3c-c4nnd56"">Cas9PTA</a>)"
                     /modified_by="thjac"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="nCAS9(D10A)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:pe-ecpo7bv"">nCAS9(D10A)</a>"
                     /label="nCAS9(D10A)"
     Linker          8373..8564
                     /created_by="warddecaestecker"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="64-aa linker"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a4-dgycryz"">64-aa linker</a>"
                     /label="64-aa linker"
     tag             8565..8630
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by
                     an enterokinase cleavage site"
                     /Transferred_Translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="3xFLAG"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:a6-dgycryz"">3xFLAG</a>"
                     /label="3xFLAG"
     CDS             8637..9260
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGE
                     RRACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAE
                     KILEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCR
                     KIFIQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
                     /label="PmCDA1"
     NLS             9264..9284
                     /created_by="warddecaestecker"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on protein similarity"
                     /Transferred_From="NLS SV40"
                     /Transferred_Translation_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; SV40 (<a
                     href=""urn:local:.:9x-dgycryz"">NLS SV40</a>)"
                     /label="SV40"
     terminator      9292..9563
                     /label="G7T"
     Linker          9568..9616
                     /created_by="wacae"
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Linker II"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:thjac:1h-c4nnd54"">Linker II</a>"
                     /label="Linker II"
     primer          complement(9677..9697)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13R"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13R
                     (pCDB-Cas9 - GWM13R)"
                     /label="GWM13R"
     promoter        9758..9808
                     /created_by="chgai"
                     /note="Derived using Geneious Prime 2022.1.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="pCG034_pKan_Kan-CBE"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ll-ch8iv1b"">pCG034_pKan_Kan-CBE</a
                     >)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lo-ch8iv1b"">pCG040_ABE-Nuc-NLS_Kan
                     -reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lr-ch8iv1b"">pCG043_Kan-ABE-Dest_co
                     ntrol_ccdB</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lu-ch8iv1c"">pCG046_ABE-LbCas12a-NL
                     S_Kan_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:kx-ch8iv19"">pCG005_pGGD-pKan-KanR-
                     E</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ky-ch8iv19"">pCG006_pGGA-Kan_promot
                     er-B</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lm-ch8iv1b"">pCG035_pKan_Kan</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:ln-ch8iv1b"">pCG039_ABE-N7-NLS_Kan-
                     reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lp-ch8iv1b"">pCG041_ABE-SV40-NLS_Ka
                     n-reporter_control</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Kan_promoter (<a
                     href=""urn:local:chgai:lw-ch8iv1c"">pCG054_Kan_CBE_control
                     </a>)"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2023.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; kan (<a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>)"
                     /label="kan"
     misc_feature    9809..10618
                     /modified_by="wacae"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KanR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:.:8j-dgycryy"">KanR</a>"
                     /label="KanR"
                     /note="Geneious type: Bacterial Selection"
     rep_origin      10765..11353
                     /created_by="thjac"
                     /modified_by="mamee"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="high-copy-number ColE1/pMB1/pBR322/pUC
                     origin of  replication"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     ColE1/pMB1/pBR322/pUC Ori (<a
                     href=""urn:local:.:8d-dk4mzop"">high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  replication</a>)"
                     /label="ColE1/pMB1/pBR322/pUC Ori"
     primer          11952..11972
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCDB-Cas9 - GWM13F"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; GWM13F
                     (pCDB-Cas9 - GWM13F)"
                     /label="GWM13F"
ORIGIN      
        1 ccgatccatt cggtccccag attagccttt tcaatttcag aaagaatgct aacccacaga
       61 tggttagaga ggcttacgca gcacgtctca tcaagacgat ctacccgagc aataatctcc
      121 aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact
      181 gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga
      241 cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg
      301 tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac
      361 tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga
      421 aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata
      481 cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc
      541 tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag
      601 gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg
      661 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      721 ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg
      781 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      841 tggagagaac acgggggacg agctaacagt attcagtcga ctggtaccaa caggctccat
      901 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      961 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1021 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1081 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1141 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1201 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     1261 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     1321 gcagaagaag acgatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     1381 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     1441 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     1501 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     1561 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtcag gttctggttc
     1621 aggttcaggt tctggatctg gaagtggatc tggatcagcg gccgcatctg aattcaagcg
     1681 tgaagagcaa gcaaggaaag ctaaggtgaa caatgagaaa aagacggaaa tagtgaaacc
     1741 agagagttgt agcaatgaag gagatgtcaa ggatctgaaa agaaaggact ctgaggatgg
     1801 aaacgagggt gaggaagaag aagcttcttc gaaaccgaaa aagccaaaag ttgctctttc
     1861 tcatcttcag gacattgacg acacagaagc tgatcaagaa gaagagtaac tgcctagagt
     1921 caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     1981 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     2041 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     2101 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     2161 tcatctatgt tactagatcg aactataagc taggtagtaa ctagtacctg tgcagcgtga
     2221 cccggtcgtg cccctctcta gagataatga gcattgcatg tctaagttat aaaaaattac
     2281 cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta tacatatatt
     2341 taaactttac tctacgaata atataatcta tagtactaca ataatatcag tgttttagag
     2401 aatcatataa atgaacagtt agacatggtc taaaggacaa ttgagtattt tgacaacagg
     2461 actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc aaatagcttc
     2521 acctatataa tacttcatcc attttattag tacatccatt tagggtttag ggttaatggt
     2581 ttttatagac taattttttt agtacatcta ttttattcta ttttagcctc taaattaaga
     2641 aaactaaaac tctattttag tttttttatt taataattta gatataaaat agaataaaat
     2701 aaagtgacta aaaattaaac aaataccctt taagaaatta aaaaaactaa ggaaacattt
     2761 ttcttgtttc gagtagataa tgccagcctg ttaaacgccg tcgacgagtc taacggacac
     2821 caaccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg
     2881 tcgctgcctc tggacccctc tcgagagttc cgctccaccg ttggacttgc tccgctgtcg
     2941 gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg cggcctcctc
     3001 ctcctctcac ggcacggcag ctacggggga ttcctttccc accgctcctt cgctttccct
     3061 tcctcgcccg ccgtaataaa tagacacccc ctccacaccc tctttcccca acctcgtgtt
     3121 gttcggagcg cacacacaca caaccagatc tcccccaaat ccacccgtcg gcacctccgc
     3181 ttcaaggtac gccgctcgtc ctcccccccc ccccctctct accttctcta gatcggcgtt
     3241 ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt
     3301 ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt
     3361 tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc
     3421 cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct
     3481 tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt
     3541 tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt
     3601 ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata
     3661 ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt
     3721 tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat
     3781 gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact
     3841 acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg
     3901 agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac
     3961 tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct
     4021 atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat
     4081 ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg
     4141 cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt ctgcaaacaa
     4201 tgaagaggcc tgcagccacc aaaaaggcag gccaggcaaa gaagaagaaa cttgacggct
     4261 cgatggataa gaagtactct atcggactcg ctatcggaac taactctgtg ggatgggctg
     4321 tgatcaccga tgagtacaag gtgccatcta agaagttcaa ggttctcgga aacaccgata
     4381 ggcactctat caagaaaaac cttatcggtg ctctcctctt cgattctggt gaaactgctg
     4441 aggctaccag actcaagaga accgctagaa gaaggtacac cagaagaaag aacaggatct
     4501 gctacctcca agagatcttc tctaacgaga tggctaaagt ggatgattca ttcttccaca
     4561 ggctcgaaga gtcattcctc gtggaagaag ataagaagca cgagaggcac cctatcttcg
     4621 gaaacatcgt tgatgaggtg gcataccacg agaagtaccc tactatctac cacctcagaa
     4681 agaagctcgt tgattctact gataaggctg atctcaggct catctacctc gctctcgctc
     4741 acatgatcaa gttcagagga cacttcctca tcgagggtga tctcaaccct gataactctg
     4801 atgtggataa gttgttcatc cagctcgtgc agacctacaa ccagcttttc gaagagaacc
     4861 ctatcaacgc ttcaggtgtg gatgctaagg ctatcctctc tgctaggctc tctaagtcaa
     4921 gaaggcttga gaacctcatt gctcagctcc ctggtgagaa gaagaacgga cttttcggaa
     4981 acttgatcgc tctctctctc ggactcaccc ctaacttcaa gtctaacttc gatctcgctg
     5041 aggatgcaaa gctccagctc tcaaaggata cctacgatga tgatctcgat aacctcctcg
     5101 ctcagatcgg agatcagtac gctgatttgt tcctcgctgc taagaacctc tctgatgcta
     5161 tcctcctcag tgatatcctc agagtgaaca ccgagatcac caaggctcca ctctcagctt
     5221 ctatgatcaa gagatacgat gagcaccacc aggatctcac acttctcaag gctcttgtta
     5281 gacagcagct cccagagaag tacaaagaga ttttcttcga tcagtctaag aacggatacg
     5341 ctggttacat cgatggtggt gcatctcaag aagagttcta caagttcatc aagcctatcc
     5401 tcgagaagat ggatggaacc gaggaactcc tcgtgaagct caatagagag gatcttctca
     5461 gaaagcagag gaccttcgat aacggatcta tccctcatca gatccacctc ggagagttgc
     5521 acgctatcct tagaaggcaa gaggatttct acccattcct caaggataac agggaaaaga
     5581 ttgagaagat tctcaccttc agaatccctt actacgtggg acctctcgct agaggaaact
     5641 caagattcgc ttggatgacc agaaagtctg aggaaaccat caccccttgg aacttcgaag
     5701 aggtggtgga taagggtgct agtgctcagt ctttcatcga gaggatgacc aacttcgata
     5761 agaaccttcc aaacgagaag gtgctcccta agcactcttt gctctacgag tacttcaccg
     5821 tgtacaacga gttgaccaag gttaagtacg tgaccgaggg aatgaggaag cctgcttttt
     5881 tgtcaggtga gcaaaagaag gctatcgttg atctcttgtt caagaccaac agaaaggtga
     5941 ccgtgaagca gctcaaagag gattacttca agaaaatcga gtgcttcgat tcagttgaga
     6001 tttctggtgt tgaggatagg ttcaacgcat ctctcggaac ctaccacgat ctcctcaaga
     6061 tcattaagga taaggatttc ttggataacg aggaaaacga ggatatcttg gaggatatcg
     6121 ttcttaccct caccctcttt gaagatagag agatgattga agaaaggctc aagacctacg
     6181 ctcatctctt cgatgataag gtgatgaagc agttgaagag aagaagatac actggttggg
     6241 gaaggctctc aagaaagctc attaacggaa tcagggataa gcagtctgga aagacaatcc
     6301 ttgatttcct caagtctgat ggattcgcta acagaaactt catgcagctc atccacgatg
     6361 attctctcac ctttaaagag gatatccaga aggctcaggt ttcaggacag ggtgatagtc
     6421 tccatgagca tatcgctaac ctcgctggat ctcctgcaat caagaaggga atcctccaga
     6481 ctgtgaaggt tgtggatgag ttggtgaagg tgatgggaag gcataagcct gagaacatcg
     6541 tgatcgaaat ggctagagag aaccagacca ctcagaaggg acagaagaac tctagggaaa
     6601 ggatgaagag gatcgaggaa ggtatcaaag agcttggatc tcagatcctc aaagagcacc
     6661 ctgttgagaa cactcagctc cagaatgaga agctctacct ctactacctc cagaacggaa
     6721 gggatatgta tgtggatcaa gagttggata tcaacaggct ctctgattac gatgttgatc
     6781 atatcgtgcc acagtcattc ttgaaggatg attctatcga taacaaggtg ctcaccaggt
     6841 ctgataagaa caggggtaag agtgataacg tgccaagtga agaggttgtg aagaaaatga
     6901 agaactattg gaggcagctc ctcaacgcta agctcatcac tcagagaaag ttcgataact
     6961 tgactaaggc tgagagggga ggactctctg aattggataa ggcaggattc atcaagaggc
     7021 agcttgtgga aaccaggcag atcactaagc acgttgcaca gatcctcgat tctaggatga
     7081 acaccaagta cgatgagaac gataagttga tcagggaagt gaaggttatc accctcaagt
     7141 caaagctcgt gtctgatttc agaaaggatt tccaattcta caaggtgagg gaaatcaaca
     7201 actaccacca cgctcacgat gcttacctta acgctgttgt tggaaccgct ctcatcaaga
     7261 agtatcctaa gctcgagtca gagttcgtgt acggtgatta caaggtgtac gatgtgagga
     7321 agatgatcgc taagtctgag caagagatcg gaaaggctac cgctaagtat ttcttctact
     7381 ctaacatcat gaatttcttc aagaccgaga ttaccctcgc taacggtgag atcagaaaga
     7441 ggccactcat cgagacaaac ggtgaaacag gtgagatcgt gtgggataag ggaagggatt
     7501 tcgctaccgt tagaaaggtg ctctctatgc cacaggtgaa catcgttaag aaaaccgagg
     7561 tgcagaccgg tggattctct aaagagtcta tcctccctaa gaggaactct gataagctca
     7621 ttgctaggaa gaaggattgg gaccctaaga aatacggtgg tttcgattct cctaccgtgg
     7681 cttactctgt tctcgttgtg gctaaggttg agaagggaaa gagtaagaag ctcaagtctg
     7741 ttaaggaact tctcggaatc actatcatgg aaaggtcatc tttcgagaag aacccaatcg
     7801 atttcctcga ggctaaggga tacaaagagg ttaagaagga tctcatcatc aagctcccaa
     7861 agtactcact cttcgaactc gagaacggta gaaagaggat gctcgcttct gctggtgagc
     7921 ttcaaaaggg aaacgagctt gctctcccat ctaagtacgt taactttctt tacctcgctt
     7981 ctcactacga gaagttgaag ggatctccag aagataacga gcagaagcaa cttttcgttg
     8041 agcagcacaa gcactacttg gatgagatca tcgagcagat ctctgagttc tctaaaaggg
     8101 tgatcctcgc tgatgcaaac ctcgataagg tgttgtctgc ttacaacaag cacagagata
     8161 agcctatcag ggaacaggca gagaacatca tccatctctt cacccttacc aacctcggtg
     8221 ctcctgctgc tttcaagtac ttcgatacaa ccatcgatag gaagagatac acctctacca
     8281 aagaagtgct cgatgctacc ctcatccatc agtctatcac tggactctac gagactagga
     8341 tcgatctctc acagctcggt ggtgattcag ctggaggagg gccaggagca gagtatgtta
     8401 gagcgttgtt tgattttaac ggtaacgatg aagaggattt accctttaag aaaggcgaca
     8461 ttctcaggat tagggataaa cctgaagagc aatggtggaa tgctgaggat agtgaaggca
     8521 aacgaggaat gattttagtg ccgtatgtgg agaaatattc gggtgactac aaagatcatg
     8581 atggtgatta caaagaccat gacatcgact acaaggatga tgatgataag tcagggatga
     8641 cagatgctga atatgtcaga atccacgaaa agttggacat ttacacgttt aagaagcagt
     8701 tcttcaacaa caagaaatct gtttcgcata ggtgctatgt gcttttcgaa ctaaaacgtc
     8761 gtggagaaag acgggcttgc ttttggggtt acgcggttaa caaaccacaa tcaggtactg
     8821 aacgaggaat acacgctgaa atcttttcta tccgaaaggt tgaggaatat ctacgtgaca
     8881 atcctggaca gttcactatc aattggtatt ctagctggtc accatgtgca gattgtgctg
     8941 agaagattct cgaatggtac aatcaagagc ttagaggcaa tggacataca ttgaaaatat
     9001 gggcatgcaa gctctactac gaaaagaatg ccagaaacca aattgggctt tggaacttga
     9061 gggataatgg agttgggctt aatgtcatgg tttctgagca ctatcaatgt tgtcggaaga
     9121 tcttcataca aagttcccat aaccagttga atgagaacag atggttagag aaaaccctta
     9181 aaagagccga gaagagaaga tccgaactga gcattatgat acaggtcaaa attctgcata
     9241 ccactaagag tccagctgta ggtcctaaga agaaacgtaa agtatgactg cccccgatga
     9301 gctaagctag ctatatcatc aatttatgta ttacacataa tatcgcactc agtctttcat
     9361 ctacggcaat gtaccagctg atataatcag ttattgaaat atttctgaat ttaaacttgc
     9421 atcaataaat ttatgttttt gcttggacta taatacctga cttgttattt tatcaataaa
     9481 tatttaaact atatttcttt caagatggga attaacatct acaaattgcc ttttcttatc
     9541 gaccatgtac tcgacggccg agtactataa gctaggtagt aactagtgcg actgtctaga
     9601 atcgcgtatg cgagtgtatc tagaatcgcg tatgcgagtg tatgagctcg atatccccta
     9661 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
     9721 tctctgatgt tacattgcac aagataaaat aatatcatca tgaacaataa aactgtctgc
     9781 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
     9841 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
     9901 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
     9961 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
    10021 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
    10081 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
    10141 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
    10201 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
    10261 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
    10321 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
    10381 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
    10441 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
    10501 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
    10561 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
    10621 agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc
    10681 aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc
    10741 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10801 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10861 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    10921 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10981 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    11041 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    11101 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    11161 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    11221 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    11281 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    11341 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    11401 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    11461 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    11521 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    11581 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    11641 taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg
    11701 atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct
    11761 ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga
    11821 gcgttcaccg acaaacaaca gataaaacga aaggcccaga cttccgactg agcctttcgt
    11881 tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca
    11941 gtcacgacgt tgtaaaacga cggccagtct taagctcggg cccacct
//
